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The map label for this gene is ptsI [H]

Identifier: 220905059

GI number: 220905059

Start: 2168337

End: 2170109

Strand: Direct

Name: ptsI [H]

Synonym: Ddes_1796

Alternate gene names: 220905059

Gene position: 2168337-2170109 (Clockwise)

Preceding gene: 220905058

Following gene: 220905060

Centisome position: 75.46

GC content: 61.87

Gene sequence:

>1773_bases
ATGGCCCGCGCGGTACTTTTCGGTACTCCCGTTTCTCCCGGCATTGCCATTGGCCGGGCGCGCTTCATGCACAAGCTGCG
GCAGGATGAAGAACGGCATATCATGCCCGCCGAAATTGCCGCCGAGCAGGAGGCCCTGCGCGCCGCGGCTGAAAGCGTAC
GCGCTTCGCTGCAGACCACCATGGACAATGTGCCGGAAGACCTGGCCGAATACCGTGAAGTGATTGCCGCACAAATGGAA
ATGGCCCGCGATGCCAAACTCCTCAATGCCGCCCTGTCACGCATAGAGCACAAACTTATTGCGGCCCCCTGGGCCTTGCG
CCTCACCGTGGAAGAACTGTGCGCCCTCTTCAGAGGCATGGATGATCCTTACCTGCGTGACCGCGCCCAGGATATCCGCG
CCGTGGGCCTGCGCCTGCGCAAACACCTGGCCATGGGACCGGTGGATGCCCCGGAAACTGCCGCCCCCGGCGTTCTGGTG
GCTGAAGACCTTTCCCCCGCTGACGTTATGGAGCTTAACCTGGACTGCGTGCTGGGCATCCTGACCACAGAGGGCGGCCC
CACTTCACATACGGCAATTCTTTCACGCAGCCTGCATATCCCTTGCCTGGCGGGCGTTACCGGCCTTGTGGCCGTAGCCA
GGGAAGACGAGCAGGTCATCGTGGACGGCCTTGGCGGCTGCGTGCTGCTGGACCCGGATGAAGCCGATCTGGCCCGCTAT
GAAAGCCGCCGCCGTGAATACATGGCGTGGGAAGAACTTACCTTGCAGACAGCCCACTGGCCTGCCGAAATGTGCGACGG
TGTGCGCGTGGTGGTGCAGGCCAACCTGGAAAGCAGCGACGAACTTGCCTCGGCACCGCGCAGCGGCGCGGACGGCGTAG
GCCTGTATCGCACAGAGTTTGCCTACCTCAAAGGGCGTTTGCCCGATGAGCAAGACCTGCTGACAGAATATGCCACGGTG
GCGCAAAAAATCGCTCCGGAGCGCGTGGTATTCCGCACGCTGGACGTGGGCGCGGACAAGATGCTGCACGCCCAGGCTGC
ACTTAAAGAGCCAAACCCGGCCCTGGGCCTGCGCGGCATACGCTTCTGCCTGCGCCATCAGGGGCTTTTCCGCACACAGC
TGCGGGCACTCATGCGGGCAGGTGTGCAGGGCAACGTGGCCATCATGCTGCCGATGATTTCAGGTCTGGACGAAGTACAG
CAGGTGCGCGGTATCATGCAGGAGCTGCATCAGGAACTGGCGGCACGGGGCCTGCCGCACGCTCCCACCCTTCCACTGGG
CGTCATGATAGAAACCCCGGCGGCGGCCATCATCTGCGATGCGCTTGCCAGGGAGTGCGATTTTTTCAGCATAGGAACCA
ATGACCTTATTCATTATATCATGGGCATAGACCGCAACAACCATCATGTGGCCTATCTTAACGAGCCGCTGCACCCCGCC
GTGGTGCGCTCGCTCAAACATATTATTGATGCCGCCCACCGTGAGGGCATTGGTGTTTCGGTCTGTGGCGAGCTTGCCTC
CGACCCGCTGGGCATGGCCCTGCTGCTCGGCATGGGGGTGGATACCATCTCTGCCGCGCCGCGCTTTGTGCCGGGCCTGA
AACACCTTATCCGCCAGCTCAAGTCCGAAACCTGCATGGAACTGGCCCACAGTGTGCTCATGAGCACAGATGTGGCCGCT
TCCCGCCGTATGGTGCGGGAAACCCTGCACCAGACCCTGGGACCGGAACTGGCTTTTCATACCACAAGCCTTTTGACCCA
CAGCCAGCCATGA

Upstream 100 bases:

>100_bases
CAGCCCGCCAATGCGGAACTGACCCTTCTGGCGCAGGGGCAGGATGCCCGCGAGGCCCTTTACGGCTTGGCCCGCCTTCT
TAACACCATGCAGGACTGAC

Downstream 100 bases:

>100_bases
GCCAGAAAACATCTCCTTCCACCATTGCCGTCAATAAGAAGGCCCGCCACCTTTATGAACTTTCAGAGTTCACCGAGGCG
GGCGTTGTGCTTACCGGACC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MARAVLFGTPVSPGIAIGRARFMHKLRQDEERHIMPAEIAAEQEALRAAAESVRASLQTTMDNVPEDLAEYREVIAAQME
MARDAKLLNAALSRIEHKLIAAPWALRLTVEELCALFRGMDDPYLRDRAQDIRAVGLRLRKHLAMGPVDAPETAAPGVLV
AEDLSPADVMELNLDCVLGILTTEGGPTSHTAILSRSLHIPCLAGVTGLVAVAREDEQVIVDGLGGCVLLDPDEADLARY
ESRRREYMAWEELTLQTAHWPAEMCDGVRVVVQANLESSDELASAPRSGADGVGLYRTEFAYLKGRLPDEQDLLTEYATV
AQKIAPERVVFRTLDVGADKMLHAQAALKEPNPALGLRGIRFCLRHQGLFRTQLRALMRAGVQGNVAIMLPMISGLDEVQ
QVRGIMQELHQELAARGLPHAPTLPLGVMIETPAAAIICDALARECDFFSIGTNDLIHYIMGIDRNNHHVAYLNEPLHPA
VVRSLKHIIDAAHREGIGVSVCGELASDPLGMALLLGMGVDTISAAPRFVPGLKHLIRQLKSETCMELAHSVLMSTDVAA
SRRMVRETLHQTLGPELAFHTTSLLTHSQP

Sequences:

>Translated_590_residues
MARAVLFGTPVSPGIAIGRARFMHKLRQDEERHIMPAEIAAEQEALRAAAESVRASLQTTMDNVPEDLAEYREVIAAQME
MARDAKLLNAALSRIEHKLIAAPWALRLTVEELCALFRGMDDPYLRDRAQDIRAVGLRLRKHLAMGPVDAPETAAPGVLV
AEDLSPADVMELNLDCVLGILTTEGGPTSHTAILSRSLHIPCLAGVTGLVAVAREDEQVIVDGLGGCVLLDPDEADLARY
ESRRREYMAWEELTLQTAHWPAEMCDGVRVVVQANLESSDELASAPRSGADGVGLYRTEFAYLKGRLPDEQDLLTEYATV
AQKIAPERVVFRTLDVGADKMLHAQAALKEPNPALGLRGIRFCLRHQGLFRTQLRALMRAGVQGNVAIMLPMISGLDEVQ
QVRGIMQELHQELAARGLPHAPTLPLGVMIETPAAAIICDALARECDFFSIGTNDLIHYIMGIDRNNHHVAYLNEPLHPA
VVRSLKHIIDAAHREGIGVSVCGELASDPLGMALLLGMGVDTISAAPRFVPGLKHLIRQLKSETCMELAHSVLMSTDVAA
SRRMVRETLHQTLGPELAFHTTSLLTHSQP
>Mature_589_residues
ARAVLFGTPVSPGIAIGRARFMHKLRQDEERHIMPAEIAAEQEALRAAAESVRASLQTTMDNVPEDLAEYREVIAAQMEM
ARDAKLLNAALSRIEHKLIAAPWALRLTVEELCALFRGMDDPYLRDRAQDIRAVGLRLRKHLAMGPVDAPETAAPGVLVA
EDLSPADVMELNLDCVLGILTTEGGPTSHTAILSRSLHIPCLAGVTGLVAVAREDEQVIVDGLGGCVLLDPDEADLARYE
SRRREYMAWEELTLQTAHWPAEMCDGVRVVVQANLESSDELASAPRSGADGVGLYRTEFAYLKGRLPDEQDLLTEYATVA
QKIAPERVVFRTLDVGADKMLHAQAALKEPNPALGLRGIRFCLRHQGLFRTQLRALMRAGVQGNVAIMLPMISGLDEVQQ
VRGIMQELHQELAARGLPHAPTLPLGVMIETPAAAIICDALARECDFFSIGTNDLIHYIMGIDRNNHHVAYLNEPLHPAV
VRSLKHIIDAAHREGIGVSVCGELASDPLGMALLLGMGVDTISAAPRFVPGLKHLIRQLKSETCMELAHSVLMSTDVAAS
RRMVRETLHQTLGPELAFHTTSLLTHSQP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=553, Percent_Identity=35.4430379746835, Blast_Score=332, Evalue=6e-92,
Organism=Escherichia coli, GI1788726, Length=556, Percent_Identity=33.8129496402878, Blast_Score=280, Evalue=3e-76,
Organism=Escherichia coli, GI1789193, Length=560, Percent_Identity=34.4642857142857, Blast_Score=276, Evalue=4e-75,
Organism=Escherichia coli, GI48994992, Length=487, Percent_Identity=32.8542094455852, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1787994, Length=450, Percent_Identity=27.1111111111111, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI226510935, Length=163, Percent_Identity=28.8343558282209, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64384; Mature: 64253

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAVLFGTPVSPGIAIGRARFMHKLRQDEERHIMPAEIAAEQEALRAAAESVRASLQTT
CCCEEEECCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MDNVPEDLAEYREVIAAQMEMARDAKLLNAALSRIEHKLIAAPWALRLTVEELCALFRGM
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
DDPYLRDRAQDIRAVGLRLRKHLAMGPVDAPETAAPGVLVAEDLSPADVMELNLDCVLGI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHCCHHHEEEE
LTTEGGPTSHTAILSRSLHIPCLAGVTGLVAVAREDEQVIVDGLGGCVLLDPDEADLARY
EECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHH
ESRRREYMAWEELTLQTAHWPAEMCDGVRVVVQANLESSDELASAPRSGADGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHH
AYLKGRLPDEQDLLTEYATVAQKIAPERVVFRTLDVGADKMLHAQAALKEPNPALGLRGI
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHH
RFCLRHQGLFRTQLRALMRAGVQGNVAIMLPMISGLDEVQQVRGIMQELHQELAARGLPH
HHHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
APTLPLGVMIETPAAAIICDALARECDFFSIGTNDLIHYIMGIDRNNHHVAYLNEPLHPA
CCCCCCCEEEECCHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCHH
VVRSLKHIIDAAHREGIGVSVCGELASDPLGMALLLGMGVDTISAAPRFVPGLKHLIRQL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
KSETCMELAHSVLMSTDVAASRRMVRETLHQTLGPELAFHTTSLLTHSQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARAVLFGTPVSPGIAIGRARFMHKLRQDEERHIMPAEIAAEQEALRAAAESVRASLQTT
CCEEEECCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MDNVPEDLAEYREVIAAQMEMARDAKLLNAALSRIEHKLIAAPWALRLTVEELCALFRGM
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
DDPYLRDRAQDIRAVGLRLRKHLAMGPVDAPETAAPGVLVAEDLSPADVMELNLDCVLGI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHCCHHHEEEE
LTTEGGPTSHTAILSRSLHIPCLAGVTGLVAVAREDEQVIVDGLGGCVLLDPDEADLARY
EECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHH
ESRRREYMAWEELTLQTAHWPAEMCDGVRVVVQANLESSDELASAPRSGADGVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHH
AYLKGRLPDEQDLLTEYATVAQKIAPERVVFRTLDVGADKMLHAQAALKEPNPALGLRGI
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHH
RFCLRHQGLFRTQLRALMRAGVQGNVAIMLPMISGLDEVQQVRGIMQELHQELAARGLPH
HHHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
APTLPLGVMIETPAAAIICDALARECDFFSIGTNDLIHYIMGIDRNNHHVAYLNEPLHPA
CCCCCCCEEEECCHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCHH
VVRSLKHIIDAAHREGIGVSVCGELASDPLGMALLLGMGVDTISAAPRFVPGLKHLIRQL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
KSETCMELAHSVLMSTDVAASRRMVRETLHQTLGPELAFHTTSLLTHSQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA