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The map label for this gene is folC [H]

Identifier: 220905003

GI number: 220905003

Start: 2103981

End: 2105315

Strand: Direct

Name: folC [H]

Synonym: Ddes_1739

Alternate gene names: 220905003

Gene position: 2103981-2105315 (Clockwise)

Preceding gene: 220905002

Following gene: 220905004

Centisome position: 73.22

GC content: 62.7

Gene sequence:

>1335_bases
ATGAAGCACTTTTTCAGCGATTTTTCCCAGATTCAACGCCACCTTGACGGCCTGGGACTTTTCCATATGGACATGGGCCT
TGACCGCATGCGGCGCGCCCTGTCCATTCTCGGCCTTGCCCGGCCCCCTTTTGTGACGGTGCAGGTTCTGGGCACCAACG
GCAAGGGATCCACTGCGGCCTTTCTTTCCTCCCTTTGCCAATCCCACGGGCTGCGCACGGGCCTGTACACCTCGCCGCAT
TTTGTCAGCCCCACGGAACGCATCCGCATTGATGGACACCCCTGGCCGCCAGACCTGTGGCCCCCCCAGGCAGATAGCAT
CATGAATGCCGTGCCGGAGCTGACCTATTTTGAATTTCTTACGGTGCTGGCTCTACTGGCTTTCAGGGAAGAACAGGTTG
ATGTGGCCGTGCTGGAAGCCGGACTCGGCGGCAGCCACGATGCCACTACGGCCGTCAGCGCGGATCTGACCTGCTTTACG
CCTATAGCGATGGATCACAAGGATGTGCTCGGCGCAGACCTTGCCGCCATCGCCAGGGACAAGGCAGGAGCCATCCGCCC
GAATGTACCGGTCTGTGCAGCCAGGCAGTTTCCACAGGCCACACGTGTGCTTGAAAATGCCGCTGCCGCCTGCAAGGCTC
CGCTCGTCTGGGCAGATACTCTTGAGGCTCCTTTTGTGCCGGGCCTTGCAGGACCTCACCAGCGTGACAATGCCGCTCTG
GCTCTCACTGCATGGCGGCGGCTGGCCCCCATGCTTGGTAAAAATCCCGCTGATGCCGCGCTGCAACAGCAGGGCCTGGC
CCGCGCTTTTGTACCCGGCCGCCTGCAGGCAATTCCCGCAGCAGACGGCCTGCCCCCCCTGCTGCTGGACGGCGCGCACA
ATCCGCACGGCATGGCCGCTCTGGTCAACGCCCTGAACCAGACAGGCGTGCGGCCCGCTGCCGCTGTTTTTTCCTGCCTG
GGGGATAAGGACTGGCATTCCGTTGCGGGCATGCTCAAAAAAATCCTGGGGCATACGCCCGTCTTTATACCAGCCCTGCA
CAATCCCCGGGCCGCCGATGTGCATGATGTTGCCGCATTTTTCAATGCCAGGCCCCCGGCCACGGCTCTGGCCGTACCGG
CCCCGACGCAATCCACGCAGGATGCGCACGGCAGCAGGCCGCACGGCTGTCTGGCTCAGGCACTGGAACAGGCCGCCGCC
TGCGCCCGGGCCGACGGCAACACCGGCCCGGTGCTGATCACAGGCTCACTCTACCTGCTGGCCGAATTTTTCAGTCTTTT
TCCCCGCCACCTTTCGCCCACAGCCGACGCATACGGGAGAAGCACCCATGAATAA

Upstream 100 bases:

>100_bases
GCTTTGGTGAGACGGCCGGCGCAGACGCTCCGCCGCCGTTTCAGGATTGAACCGCTCTAAAACGCTGACAGTTGCGCGCC
TTTGCGTTACCGTGCTGTGC

Downstream 100 bases:

>100_bases
TCTCTTCCGCTCCATCCCCGCCGTGGATCTGTGCCTTACGGCCCTCGGCCAGGCCGATCCGTCCCTGGTCGAAGCTCCGC
GCGGCCTGATGCGCGACCTT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKHFFSDFSQIQRHLDGLGLFHMDMGLDRMRRALSILGLARPPFVTVQVLGTNGKGSTAAFLSSLCQSHGLRTGLYTSPH
FVSPTERIRIDGHPWPPDLWPPQADSIMNAVPELTYFEFLTVLALLAFREEQVDVAVLEAGLGGSHDATTAVSADLTCFT
PIAMDHKDVLGADLAAIARDKAGAIRPNVPVCAARQFPQATRVLENAAAACKAPLVWADTLEAPFVPGLAGPHQRDNAAL
ALTAWRRLAPMLGKNPADAALQQQGLARAFVPGRLQAIPAADGLPPLLLDGAHNPHGMAALVNALNQTGVRPAAAVFSCL
GDKDWHSVAGMLKKILGHTPVFIPALHNPRAADVHDVAAFFNARPPATALAVPAPTQSTQDAHGSRPHGCLAQALEQAAA
CARADGNTGPVLITGSLYLLAEFFSLFPRHLSPTADAYGRSTHE

Sequences:

>Translated_444_residues
MKHFFSDFSQIQRHLDGLGLFHMDMGLDRMRRALSILGLARPPFVTVQVLGTNGKGSTAAFLSSLCQSHGLRTGLYTSPH
FVSPTERIRIDGHPWPPDLWPPQADSIMNAVPELTYFEFLTVLALLAFREEQVDVAVLEAGLGGSHDATTAVSADLTCFT
PIAMDHKDVLGADLAAIARDKAGAIRPNVPVCAARQFPQATRVLENAAAACKAPLVWADTLEAPFVPGLAGPHQRDNAAL
ALTAWRRLAPMLGKNPADAALQQQGLARAFVPGRLQAIPAADGLPPLLLDGAHNPHGMAALVNALNQTGVRPAAAVFSCL
GDKDWHSVAGMLKKILGHTPVFIPALHNPRAADVHDVAAFFNARPPATALAVPAPTQSTQDAHGSRPHGCLAQALEQAAA
CARADGNTGPVLITGSLYLLAEFFSLFPRHLSPTADAYGRSTHE
>Mature_444_residues
MKHFFSDFSQIQRHLDGLGLFHMDMGLDRMRRALSILGLARPPFVTVQVLGTNGKGSTAAFLSSLCQSHGLRTGLYTSPH
FVSPTERIRIDGHPWPPDLWPPQADSIMNAVPELTYFEFLTVLALLAFREEQVDVAVLEAGLGGSHDATTAVSADLTCFT
PIAMDHKDVLGADLAAIARDKAGAIRPNVPVCAARQFPQATRVLENAAAACKAPLVWADTLEAPFVPGLAGPHQRDNAAL
ALTAWRRLAPMLGKNPADAALQQQGLARAFVPGRLQAIPAADGLPPLLLDGAHNPHGMAALVNALNQTGVRPAAAVFSCL
GDKDWHSVAGMLKKILGHTPVFIPALHNPRAADVHDVAAFFNARPPATALAVPAPTQSTQDAHGSRPHGCLAQALEQAAA
CARADGNTGPVLITGSLYLLAEFFSLFPRHLSPTADAYGRSTHE

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=368, Percent_Identity=32.0652173913043, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI66932984, Length=368, Percent_Identity=32.0652173913043, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1788654, Length=437, Percent_Identity=30.4347826086957, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71984923, Length=459, Percent_Identity=28.9760348583878, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=459, Percent_Identity=28.9760348583878, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=459, Percent_Identity=28.9760348583878, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=443, Percent_Identity=26.1851015801354, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=290, Percent_Identity=31.3793103448276, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24641571, Length=414, Percent_Identity=29.951690821256, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581568, Length=175, Percent_Identity=34.2857142857143, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47113; Mature: 47113

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFFSDFSQIQRHLDGLGLFHMDMGLDRMRRALSILGLARPPFVTVQVLGTNGKGSTAA
CCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHH
FLSSLCQSHGLRTGLYTSPHFVSPTERIRIDGHPWPPDLWPPQADSIMNAVPELTYFEFL
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TVLALLAFREEQVDVAVLEAGLGGSHDATTAVSADLTCFTPIAMDHKDVLGADLAAIARD
HHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHC
KAGAIRPNVPVCAARQFPQATRVLENAAAACKAPLVWADTLEAPFVPGLAGPHQRDNAAL
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHH
ALTAWRRLAPMLGKNPADAALQQQGLARAFVPGRLQAIPAADGLPPLLLDGAHNPHGMAA
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCEECCCCCCHHHHH
LVNALNQTGVRPAAAVFSCLGDKDWHSVAGMLKKILGHTPVFIPALHNPRAADVHDVAAF
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHH
FNARPPATALAVPAPTQSTQDAHGSRPHGCLAQALEQAAACARADGNTGPVLITGSLYLL
HCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHH
AEFFSLFPRHLSPTADAYGRSTHE
HHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MKHFFSDFSQIQRHLDGLGLFHMDMGLDRMRRALSILGLARPPFVTVQVLGTNGKGSTAA
CCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHH
FLSSLCQSHGLRTGLYTSPHFVSPTERIRIDGHPWPPDLWPPQADSIMNAVPELTYFEFL
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TVLALLAFREEQVDVAVLEAGLGGSHDATTAVSADLTCFTPIAMDHKDVLGADLAAIARD
HHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHC
KAGAIRPNVPVCAARQFPQATRVLENAAAACKAPLVWADTLEAPFVPGLAGPHQRDNAAL
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHH
ALTAWRRLAPMLGKNPADAALQQQGLARAFVPGRLQAIPAADGLPPLLLDGAHNPHGMAA
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCEECCCCCCHHHHH
LVNALNQTGVRPAAAVFSCLGDKDWHSVAGMLKKILGHTPVFIPALHNPRAADVHDVAAF
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHH
FNARPPATALAVPAPTQSTQDAHGSRPHGCLAQALEQAAACARADGNTGPVLITGSLYLL
HCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHH
AEFFSLFPRHLSPTADAYGRSTHE
HHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]