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The map label for this gene is selA

Identifier: 220905004

GI number: 220905004

Start: 2105308

End: 2106735

Strand: Direct

Name: selA

Synonym: Ddes_1740

Alternate gene names: 220905004

Gene position: 2105308-2106735 (Clockwise)

Preceding gene: 220905003

Following gene: 220905005

Centisome position: 73.27

GC content: 62.82

Gene sequence:

>1428_bases
ATGAATAATCTCTTCCGCTCCATCCCCGCCGTGGATCTGTGCCTTACGGCCCTCGGCCAGGCCGATCCGTCCCTGGTCGA
AGCTCCGCGCGGCCTGATGCGCGACCTTATAACGGAGTTCTGGGACAAAAAACGCCACGAAATACGCGAAGGCCATTGCA
AAAAAAACGAACAACTGACCCTTGATGCCTGCCTGCCCGGGCTCATGACGCATGTGCGCGCAGGTCTGCGCAGCCGCTTC
CGGGCAGCGCTCAATGCTACCGGCGTTGTGGTGCATACCAACATGGGGCGTTCCGTGCTTGCCAAAGAGGCGCGCGAAGC
GGTCATTACCGCCGCCACCGGCTATTGCAACCTGGAGCTGGATATGCAAAGCGGCGGCCGGGGCAGCCGTCATGCCCTGG
TTGAAGACCTGCTGTGCCGCCTGACCGGGGCAGAAGCCGCCCTTGTGGTCAACAATAACGCCGCCGCCGTGCTTCTGGTG
CTGGACACCTTTTGCAAAGGCGGCGAAGTGGTTGTTTCACGCGGCGAACTGGTGGAAATCGGCGGCAGTTTCCGTATCCC
CGAAGTGATGGAAAAAAGCGGAGCCACACTGCGCGAGGTGGGGGCTACCAACCGCACCCATCTGCACGACTACAGCGCGG
CCATAAATGAAAACACCCGCGCGCTCATGCGGGTGCACACGTCAAACTACCGCATTGTTGGCTTTCATTCCGCCGTGCCG
CTGCCCGAGCTGGCGGCCCTGGCGCGCCGGCATGATCTGCCCCTTATCGAAGACCTCGGCAGCGGCAGCCTGACGGACTT
TTCTTCCTGCGGCCTGCCCGATGAGCCAACAGTCCCCTCAGTGCTGGCGCAAGGGGCGGACATCGCCACATTTTCCGGCG
ACAAGGTGCTCGGTGGCCCGCAAGCGGGCATTATCACAGGGCGTAAGGACATGGTCGACACACTCAAGCGCAATCCGCTC
ACCCGCGCCCTGCGCTGCGATAAACTCTGCCTGGCCGGGCTTGAGGCCACATTGCGCCTCTATCTTGACCCCGAAAAAGC
CCGTCAGAAAATCCCCACCCTACGCATGATCTGCCGCCCGGCCGAGGAGCTGGCGCGCGCTGCGCGCACCTTGGCCGCGG
CGCTGCGCAAGGGGCTTGCCGGTTCGTCGGCGTCCTGCCTTGTAAGCCTGCGGGCCGACGTTTCGCGCGTGGGCGGAGGG
GCATTTCCGCAATACGACCTGCCCACGACGCTTGTGTGCCTCAAGCCTGAAAACTGCAGTGCAACGGCTCTCAAGGCGGC
CCTTCTGCGCACTGATCCGCCCCTTATAGGACGGCTGGAAGACGACAGTTTCTGCCTTGACCCGCGTACCCTGAGCGCCG
AAGACAGGCCCACGCTGCTGCGCGTACTGCGCGAGGCTCTGGCTTTGGCCGCAAAAGAGATTATATAG

Upstream 100 bases:

>100_bases
CCGGTGCTGATCACAGGCTCACTCTACCTGCTGGCCGAATTTTTCAGTCTTTTTCCCCGCCACCTTTCGCCCACAGCCGA
CGCATACGGGAGAAGCACCC

Downstream 100 bases:

>100_bases
GCGGTACTGACCGCAAAAATGACAGGCCTGCCGTTCGGGCAGGCCGTAAAGGAAAACAGCATGGAAAAGACTCTGGCCTA
CAAACCCCAAAACGCCTGGG

Product: selenocysteine synthase

Products: NA

Alternate protein names: Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNNLFRSIPAVDLCLTALGQADPSLVEAPRGLMRDLITEFWDKKRHEIREGHCKKNEQLTLDACLPGLMTHVRAGLRSRF
RAALNATGVVVHTNMGRSVLAKEAREAVITAATGYCNLELDMQSGGRGSRHALVEDLLCRLTGAEAALVVNNNAAAVLLV
LDTFCKGGEVVVSRGELVEIGGSFRIPEVMEKSGATLREVGATNRTHLHDYSAAINENTRALMRVHTSNYRIVGFHSAVP
LPELAALARRHDLPLIEDLGSGSLTDFSSCGLPDEPTVPSVLAQGADIATFSGDKVLGGPQAGIITGRKDMVDTLKRNPL
TRALRCDKLCLAGLEATLRLYLDPEKARQKIPTLRMICRPAEELARAARTLAAALRKGLAGSSASCLVSLRADVSRVGGG
AFPQYDLPTTLVCLKPENCSATALKAALLRTDPPLIGRLEDDSFCLDPRTLSAEDRPTLLRVLREALALAAKEII

Sequences:

>Translated_475_residues
MNNLFRSIPAVDLCLTALGQADPSLVEAPRGLMRDLITEFWDKKRHEIREGHCKKNEQLTLDACLPGLMTHVRAGLRSRF
RAALNATGVVVHTNMGRSVLAKEAREAVITAATGYCNLELDMQSGGRGSRHALVEDLLCRLTGAEAALVVNNNAAAVLLV
LDTFCKGGEVVVSRGELVEIGGSFRIPEVMEKSGATLREVGATNRTHLHDYSAAINENTRALMRVHTSNYRIVGFHSAVP
LPELAALARRHDLPLIEDLGSGSLTDFSSCGLPDEPTVPSVLAQGADIATFSGDKVLGGPQAGIITGRKDMVDTLKRNPL
TRALRCDKLCLAGLEATLRLYLDPEKARQKIPTLRMICRPAEELARAARTLAAALRKGLAGSSASCLVSLRADVSRVGGG
AFPQYDLPTTLVCLKPENCSATALKAALLRTDPPLIGRLEDDSFCLDPRTLSAEDRPTLLRVLREALALAAKEII
>Mature_475_residues
MNNLFRSIPAVDLCLTALGQADPSLVEAPRGLMRDLITEFWDKKRHEIREGHCKKNEQLTLDACLPGLMTHVRAGLRSRF
RAALNATGVVVHTNMGRSVLAKEAREAVITAATGYCNLELDMQSGGRGSRHALVEDLLCRLTGAEAALVVNNNAAAVLLV
LDTFCKGGEVVVSRGELVEIGGSFRIPEVMEKSGATLREVGATNRTHLHDYSAAINENTRALMRVHTSNYRIVGFHSAVP
LPELAALARRHDLPLIEDLGSGSLTDFSSCGLPDEPTVPSVLAQGADIATFSGDKVLGGPQAGIITGRKDMVDTLKRNPL
TRALRCDKLCLAGLEATLRLYLDPEKARQKIPTLRMICRPAEELARAARTLAAALRKGLAGSSASCLVSLRADVSRVGGG
AFPQYDLPTTLVCLKPENCSATALKAALLRTDPPLIGRLEDDSFCLDPRTLSAEDRPTLLRVLREALALAAKEII

Specific function: Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis

COG id: COG1921

COG function: function code E; Selenocysteine synthase [seryl-tRNASer selenium transferase]

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the selA family

Homologues:

Organism=Escherichia coli, GI2367248, Length=465, Percent_Identity=46.0215053763441, Blast_Score=331, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELA_DESDA (B8J1N0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480316.1
- GeneID:   7285452
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1740
- HOGENOM:   HBG564950
- ProtClustDB:   PRK04311
- GO:   GO:0005737
- HAMAP:   MF_00423
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR018319
- InterPro:   IPR004534
- Gene3D:   G3DSA:3.40.640.10
- TIGRFAMs:   TIGR00474

Pfam domain/function: PF03841 SelA; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.9.1.1

Molecular weight: Translated: 51085; Mature: 51085

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLFRSIPAVDLCLTALGQADPSLVEAPRGLMRDLITEFWDKKRHEIREGHCKKNEQLT
CCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LDACLPGLMTHVRAGLRSRFRAALNATGVVVHTNMGRSVLAKEAREAVITAATGYCNLEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEEECCEEEEEE
DMQSGGRGSRHALVEDLLCRLTGAEAALVVNNNAAAVLLVLDTFCKGGEVVVSRGELVEI
ECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHCCCCCEEEECCCEEEE
GGSFRIPEVMEKSGATLREVGATNRTHLHDYSAAINENTRALMRVHTSNYRIVGFHSAVP
CCCCCCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCC
LPELAALARRHDLPLIEDLGSGSLTDFSSCGLPDEPTVPSVLAQGADIATFSGDKVLGGP
CHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCC
QAGIITGRKDMVDTLKRNPLTRALRCDKLCLAGLEATLRLYLDPEKARQKIPTLRMICRP
CCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHCCHHHHHHCC
AEELARAARTLAAALRKGLAGSSASCLVSLRADVSRVGGGAFPQYDLPTTLVCLKPENCS
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
ATALKAALLRTDPPLIGRLEDDSFCLDPRTLSAEDRPTLLRVLREALALAAKEII
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLFRSIPAVDLCLTALGQADPSLVEAPRGLMRDLITEFWDKKRHEIREGHCKKNEQLT
CCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LDACLPGLMTHVRAGLRSRFRAALNATGVVVHTNMGRSVLAKEAREAVITAATGYCNLEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEEECCEEEEEE
DMQSGGRGSRHALVEDLLCRLTGAEAALVVNNNAAAVLLVLDTFCKGGEVVVSRGELVEI
ECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHCCCCCEEEECCCEEEE
GGSFRIPEVMEKSGATLREVGATNRTHLHDYSAAINENTRALMRVHTSNYRIVGFHSAVP
CCCCCCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCC
LPELAALARRHDLPLIEDLGSGSLTDFSSCGLPDEPTVPSVLAQGADIATFSGDKVLGGP
CHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCC
QAGIITGRKDMVDTLKRNPLTRALRCDKLCLAGLEATLRLYLDPEKARQKIPTLRMICRP
CCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHCCHHHHHHCC
AEELARAARTLAAALRKGLAGSSASCLVSLRADVSRVGGGAFPQYDLPTTLVCLKPENCS
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
ATALKAALLRTDPPLIGRLEDDSFCLDPRTLSAEDRPTLLRVLREALALAAKEII
HHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA