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The map label for this gene is ndhI [H]

Identifier: 220904933

GI number: 220904933

Start: 2005802

End: 2006203

Strand: Reverse

Name: ndhI [H]

Synonym: Ddes_1668

Alternate gene names: 220904933

Gene position: 2006203-2005802 (Counterclockwise)

Preceding gene: 220904934

Following gene: 220904932

Centisome position: 69.82

GC content: 53.23

Gene sequence:

>402_bases
ATGTATATGCTCAAGAACGTGCTGCGCAACCTGTCAGGCAAGCCTGCCACAAGGCTGTATCCGCTGGAAGAGCGTGAACC
TTTTCCGGCCTACAGGGGCGTAATCACCAATGAAGTGGAGAAGTGCATATTCTGCAATAGCTGCGCAAGGGTTTGCCCCA
CAGATGCCATTACTGTGGACGCCAAGGCCGGGCATTGGCATTACGACCCCTTCTTGTGTGTGTACTGCTCGGCCTGTGTA
GAAAAATGCCCAACAAAGTGTCTGGTGCAGGTTCCCACGCACCGCAAACCTTCGGTAACCAAATTCCGTGTTCTGCGCAC
GGGTACGCCGCGTGTTAAAAAATCCGCGGCAGCCAAGGGCAAGGAAGAAAGCGGAATAGAAAAGAAGGCAGAGAAAGAGT
AG

Upstream 100 bases:

>100_bases
TCCGCCCCTGCTGCACATGTTGCCGGGCTGCAAGTTGCCCGACGTGCCGGTTATTGTGCTCAGTATCGACCCGTGCATTT
CCTGCACAGAGAGGTAGCCC

Downstream 100 bases:

>100_bases
GCGTGGCGTTGGCCGCGCGAACACATAACAACGTTTTGTTATGATCAGCAAAAAGCCGCCGGAAGGCGGCTTTTTGCTTT
GTGCATGCCATCGGGATGGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MYMLKNVLRNLSGKPATRLYPLEEREPFPAYRGVITNEVEKCIFCNSCARVCPTDAITVDAKAGHWHYDPFLCVYCSACV
EKCPTKCLVQVPTHRKPSVTKFRVLRTGTPRVKKSAAAKGKEESGIEKKAEKE

Sequences:

>Translated_133_residues
MYMLKNVLRNLSGKPATRLYPLEEREPFPAYRGVITNEVEKCIFCNSCARVCPTDAITVDAKAGHWHYDPFLCVYCSACV
EKCPTKCLVQVPTHRKPSVTKFRVLRTGTPRVKKSAAAKGKEESGIEKKAEKE
>Mature_133_residues
MYMLKNVLRNLSGKPATRLYPLEEREPFPAYRGVITNEVEKCIFCNSCARVCPTDAITVDAKAGHWHYDPFLCVYCSACV
EKCPTKCLVQVPTHRKPSVTKFRVLRTGTPRVKKSAAAKGKEESGIEKKAEKE

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 14965; Mature: 14965

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMLKNVLRNLSGKPATRLYPLEEREPFPAYRGVITNEVEKCIFCNSCARVCPTDAITVD
CHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
AKAGHWHYDPFLCVYCSACVEKCPTKCLVQVPTHRKPSVTKFRVLRTGTPRVKKSAAAKG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCC
KEESGIEKKAEKE
CCCCCCCCCCCCC
>Mature Secondary Structure
MYMLKNVLRNLSGKPATRLYPLEEREPFPAYRGVITNEVEKCIFCNSCARVCPTDAITVD
CHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
AKAGHWHYDPFLCVYCSACVEKCPTKCLVQVPTHRKPSVTKFRVLRTGTPRVKKSAAAKG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCC
KEESGIEKKAEKE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA