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The map label for this gene is cooH [H]

Identifier: 220904934

GI number: 220904934

Start: 2006207

End: 2007289

Strand: Reverse

Name: cooH [H]

Synonym: Ddes_1669

Alternate gene names: 220904934

Gene position: 2007289-2006207 (Counterclockwise)

Preceding gene: 220904935

Following gene: 220904933

Centisome position: 69.86

GC content: 59.37

Gene sequence:

>1083_bases
ATGAGCAACCGCACCACAGTCATTCCTTTCGGGCCGCAGCACCCCGTGCTGCCCGAGCCGCTGCACATCAAGTTTGTGGT
GGAGGACGAAACCGTTGTCGGGGCTATCCCGCAGCTTGGTTTTGTGCACCGTGGGCTTGAAAGTCTCGTGCGCACCAAGG
ACTACAACCAGATGGTCTTTGTGGTGGAGCGCATCTGCGGCATCTGCTCCTGCATCCACGCCAACTGCTATTGCAACGCC
CTTGAAAATATGATGGGCATCACGGCTCCGCCGCGTGCCCAGTTTCTGCGGGTTATCTGGTCAGAGCTGCACCGCATCCA
TTCGCATCTTCTCTGGCTCGGCCTTTTTGCCGATGCCTTTGGCTTCGAAAGCGTGTTCCAGCAGTTCTGGCGTATCCGCG
AACATGTTATGGATATCTGCGAAGCCACGGCAGGCAACCGCGTTATCCTGTCGGTCAACGTGGTGGGCGGCGTGCGCCGC
GACCTGGCGCCCGACCAGATACGCTGGATGCTCGGTCGGCTTGATGAACTGCAAAAGGGCATGCGTGAGCTCACAACCAC
CATGCTTGACGACTACACCGTGCAGGAGCGTACGCGCGGCATAGGTTATCTCAGTAAAGACGATGCCCGGCTGCTGGGCG
CCGCCGGACCGACCCTGCGCGGCAGCGGCTGGGAAATCGACGAGCGTATGCACGGCTATGCCGCCTACAACGATCTTAAC
TTCATTCCTGTGGTCGAACACGATGGCGACTGCTATGCCCGCGCCAAGGTACGCTTCTACGAAGTGCTGCACGCCATGGA
CCTTATCCGCGAAGCGCTGAACCGCCTGCCGGAAAGCGAACTTACGGTGAAAGTTCCCGGCAACCCCGATGGCGAAGCTG
TTTTCCGGGTGGAACAGCCTAGGGGCGAACTGTTCTATTACATACGGGCCAACGGGACCAAGTATCTGGAGCGCATGCGT
GTGCGTACGCCCACCTTTGCCAACGTTCCGCCCCTGCTGCACATGTTGCCGGGCTGCAAGTTGCCCGACGTGCCGGTTAT
TGTGCTCAGTATCGACCCGTGCATTTCCTGCACAGAGAGGTAG

Upstream 100 bases:

>100_bases
ATCTGGAAGGCGAAGTGACCCACGCCCCGTACTTCACCATGACGACCGTCCGGCGTCCCGCCGCGTCTGCCAAGGCAGAG
CCCGCCAAAGGAGATCAGGC

Downstream 100 bases:

>100_bases
CCCATGTATATGCTCAAGAACGTGCTGCGCAACCTGTCAGGCAAGCCTGCCACAAGGCTGTATCCGCTGGAAGAGCGTGA
ACCTTTTCCGGCCTACAGGG

Product: NADH-ubiquinone oxidoreductase chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSNRTTVIPFGPQHPVLPEPLHIKFVVEDETVVGAIPQLGFVHRGLESLVRTKDYNQMVFVVERICGICSCIHANCYCNA
LENMMGITAPPRAQFLRVIWSELHRIHSHLLWLGLFADAFGFESVFQQFWRIREHVMDICEATAGNRVILSVNVVGGVRR
DLAPDQIRWMLGRLDELQKGMRELTTTMLDDYTVQERTRGIGYLSKDDARLLGAAGPTLRGSGWEIDERMHGYAAYNDLN
FIPVVEHDGDCYARAKVRFYEVLHAMDLIREALNRLPESELTVKVPGNPDGEAVFRVEQPRGELFYYIRANGTKYLERMR
VRTPTFANVPPLLHMLPGCKLPDVPVIVLSIDPCISCTER

Sequences:

>Translated_360_residues
MSNRTTVIPFGPQHPVLPEPLHIKFVVEDETVVGAIPQLGFVHRGLESLVRTKDYNQMVFVVERICGICSCIHANCYCNA
LENMMGITAPPRAQFLRVIWSELHRIHSHLLWLGLFADAFGFESVFQQFWRIREHVMDICEATAGNRVILSVNVVGGVRR
DLAPDQIRWMLGRLDELQKGMRELTTTMLDDYTVQERTRGIGYLSKDDARLLGAAGPTLRGSGWEIDERMHGYAAYNDLN
FIPVVEHDGDCYARAKVRFYEVLHAMDLIREALNRLPESELTVKVPGNPDGEAVFRVEQPRGELFYYIRANGTKYLERMR
VRTPTFANVPPLLHMLPGCKLPDVPVIVLSIDPCISCTER
>Mature_359_residues
SNRTTVIPFGPQHPVLPEPLHIKFVVEDETVVGAIPQLGFVHRGLESLVRTKDYNQMVFVVERICGICSCIHANCYCNAL
ENMMGITAPPRAQFLRVIWSELHRIHSHLLWLGLFADAFGFESVFQQFWRIREHVMDICEATAGNRVILSVNVVGGVRRD
LAPDQIRWMLGRLDELQKGMRELTTTMLDDYTVQERTRGIGYLSKDDARLLGAAGPTLRGSGWEIDERMHGYAAYNDLNF
IPVVEHDGDCYARAKVRFYEVLHAMDLIREALNRLPESELTVKVPGNPDGEAVFRVEQPRGELFYYIRANGTKYLERMRV
RTPTFANVPPLLHMLPGCKLPDVPVIVLSIDPCISCTER

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=393, Percent_Identity=26.4631043256997, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI260898743, Length=381, Percent_Identity=26.7716535433071, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1789076, Length=362, Percent_Identity=37.5690607734807, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1788832, Length=356, Percent_Identity=37.0786516853933, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI145693162, Length=382, Percent_Identity=27.4869109947644, Blast_Score=151, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17555284, Length=390, Percent_Identity=27.1794871794872, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17568379, Length=390, Percent_Identity=27.1794871794872, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI221459469, Length=380, Percent_Identity=27.1052631578947, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24638644, Length=388, Percent_Identity=27.0618556701031, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 40919; Mature: 40788

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRTTVIPFGPQHPVLPEPLHIKFVVEDETVVGAIPQLGFVHRGLESLVRTKDYNQMVF
CCCCCEEEECCCCCCCCCCCEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
VVERICGICSCIHANCYCNALENMMGITAPPRAQFLRVIWSELHRIHSHLLWLGLFADAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFESVFQQFWRIREHVMDICEATAGNRVILSVNVVGGVRRDLAPDQIRWMLGRLDELQKG
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
MRELTTTMLDDYTVQERTRGIGYLSKDDARLLGAAGPTLRGSGWEIDERMHGYAAYNDLN
HHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCEEEECCCC
FIPVVEHDGDCYARAKVRFYEVLHAMDLIREALNRLPESELTVKVPGNPDGEAVFRVEQP
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECC
RGELFYYIRANGTKYLERMRVRTPTFANVPPLLHMLPGCKLPDVPVIVLSIDPCISCTER
CCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
SNRTTVIPFGPQHPVLPEPLHIKFVVEDETVVGAIPQLGFVHRGLESLVRTKDYNQMVF
CCCCEEEECCCCCCCCCCCEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
VVERICGICSCIHANCYCNALENMMGITAPPRAQFLRVIWSELHRIHSHLLWLGLFADAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFESVFQQFWRIREHVMDICEATAGNRVILSVNVVGGVRRDLAPDQIRWMLGRLDELQKG
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
MRELTTTMLDDYTVQERTRGIGYLSKDDARLLGAAGPTLRGSGWEIDERMHGYAAYNDLN
HHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCEEEECCCC
FIPVVEHDGDCYARAKVRFYEVLHAMDLIREALNRLPESELTVKVPGNPDGEAVFRVEQP
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECC
RGELFYYIRANGTKYLERMRVRTPTFANVPPLLHMLPGCKLPDVPVIVLSIDPCISCTER
CCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]