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The map label for this gene is tuf

Identifier: 220904893

GI number: 220904893

Start: 1955853

End: 1957046

Strand: Direct

Name: tuf

Synonym: Ddes_1628

Alternate gene names: 220904893

Gene position: 1955853-1957046 (Clockwise)

Preceding gene: 220904892

Following gene: 220904894

Centisome position: 68.07

GC content: 59.72

Gene sequence:

>1194_bases
ATGGGCAAGGAAAAATTTGAACGCAAAAAGCCCCATGTAAACATCGGCACCATCGGCCACATTGACCATGGCAAAACCAC
GTTGACCGCGGCCATCACCAAAGTGGCCAACCTGAAAAGCGGCGGCAAGTTCATTTCGTATGACGAAATCGACAAGGCCC
CCGAAGAAAAGGAACGCGGCATCACCATTGCCACCTCGCACGTTGAGTACGAGACCGACAAGCGTCACTACGCTCACGTG
GACTGCCCCGGTCACGCCGACTACATCAAGAACATGATCACCGGCGCTGCCCAGATGGACGGCGGCATCCTGGTGGTGGC
TGCCACTGACGGCCCCATGCCCCAGACCCGTGAGCACATCCTGCTCGCCCGTCAGGTCGGTGTGCCCCAGCTGGTGGTGT
TCCTGAACAAGTGCGACCTCGTGGACGACGAAGAGCTGCTCGAACTGGTGGAGCTGGAAGTGCGCGAACTGCTCTCTTCC
TACGACTTCCCCGGCGACGACGTGCCCGTGATCCGCGGCTCCGCACTCAAGGCCCTGGAATCCGATGATCCCAACTCCGC
TGAAGCCAAGTGCATTCTTGACCTTCTGGATGCTTGCGACTCCTTCATTCCCGAGCCTGTGCGTGACATTGACAAGCCCT
TCCTGATGCCCATTGAAGACGTGTTCTCCATCTCCGGCCGCGGCACCGTTGTGACCGGTCGTGTGGAACGCGGCGTCATC
AAGGTCGGCGAAGAAGTGGAAATCGTGGGCATCAAGCCCACCGTCAAGACCACCTGCACCGGCGTTGAAATGTTCCGCAA
GCTGCTCGACCAGGGCGAAGCCGGCGACAACATCGGCGCCCTGCTGCGCGGCACCAAGCGTGACGATGTGGAACGCGGTC
AGGTTCTTGCCGCGCCCAAGAGCATCACGCCCCACAAGAAGTTCAAGGCTGAAGTGTACGTGCTCTCCAAGGAAGAAGGC
GGCCGCCATACCCCGTTCTTCACCGGATACCGTCCCCAGTTCTACTTCCGTACCACGGACATCACCGGCGTCATCAGCCT
GCCCGAAGGTGTGGAAATGGTTATGCCTGGCGACAACTCGCAGTTTATTGTTGAACTCATCGCTCCCATCGCCATGGAAG
CCGGCCTGCGCTTCGCCATCCGTGAAGGTGGCCGCACCGTTGGCTCGGGCGTGGTGACCGAAATCAACGAGTAG

Upstream 100 bases:

>100_bases
AGCCGTTACGCGAGGAGCAGGCGTTACACACGCAAGGCTTGAAGCACTTTTTTGTAACGCAGGTAAAACGCAAACATTAT
TTTCTCCAGGGAGACGTACA

Downstream 100 bases:

>100_bases
TTTTATGAGAGTCAACATACTTCTGGCTTGCACTGAGTGCAAACGCCGCAACTACAGCACCCGGAAGAACAAGAAAAATA
CTACCGGTCGGCTGGAAATG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MGKEKFERKKPHVNIGTIGHIDHGKTTLTAAITKVANLKSGGKFISYDEIDKAPEEKERGITIATSHVEYETDKRHYAHV
DCPGHADYIKNMITGAAQMDGGILVVAATDGPMPQTREHILLARQVGVPQLVVFLNKCDLVDDEELLELVELEVRELLSS
YDFPGDDVPVIRGSALKALESDDPNSAEAKCILDLLDACDSFIPEPVRDIDKPFLMPIEDVFSISGRGTVVTGRVERGVI
KVGEEVEIVGIKPTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEG
GRHTPFFTGYRPQFYFRTTDITGVISLPEGVEMVMPGDNSQFIVELIAPIAMEAGLRFAIREGGRTVGSGVVTEINE

Sequences:

>Translated_397_residues
MGKEKFERKKPHVNIGTIGHIDHGKTTLTAAITKVANLKSGGKFISYDEIDKAPEEKERGITIATSHVEYETDKRHYAHV
DCPGHADYIKNMITGAAQMDGGILVVAATDGPMPQTREHILLARQVGVPQLVVFLNKCDLVDDEELLELVELEVRELLSS
YDFPGDDVPVIRGSALKALESDDPNSAEAKCILDLLDACDSFIPEPVRDIDKPFLMPIEDVFSISGRGTVVTGRVERGVI
KVGEEVEIVGIKPTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEG
GRHTPFFTGYRPQFYFRTTDITGVISLPEGVEMVMPGDNSQFIVELIAPIAMEAGLRFAIREGGRTVGSGVVTEINE
>Mature_396_residues
GKEKFERKKPHVNIGTIGHIDHGKTTLTAAITKVANLKSGGKFISYDEIDKAPEEKERGITIATSHVEYETDKRHYAHVD
CPGHADYIKNMITGAAQMDGGILVVAATDGPMPQTREHILLARQVGVPQLVVFLNKCDLVDDEELLELVELEVRELLSSY
DFPGDDVPVIRGSALKALESDDPNSAEAKCILDLLDACDSFIPEPVRDIDKPFLMPIEDVFSISGRGTVVTGRVERGVIK
VGEEVEIVGIKPTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEGG
RHTPFFTGYRPQFYFRTTDITGVISLPEGVEMVMPGDNSQFIVELIAPIAMEAGLRFAIREGGRTVGSGVVTEINE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=57.6530612244898, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.5192743764172, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.2925170068027, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI4503471, Length=465, Percent_Identity=31.6129032258064, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=30.9050772626932, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=30.4347826086957, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=25.5760368663594, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=25.5760368663594, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=25.5760368663594, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI46094014, Length=379, Percent_Identity=28.2321899736148, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=285, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=293, Percent_Identity=25.2559726962457, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.7215189873418, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.7215189873418, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=29.2553191489362, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=32.3636363636364, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789108, Length=433, Percent_Identity=27.0207852193995, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.7363184079602, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=47.4358974358974, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI17552884, Length=458, Percent_Identity=31.8777292576419, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=458, Percent_Identity=31.8777292576419, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=30.6306306306306, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=29.7482837528604, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=29.7482837528604, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=343, Percent_Identity=28.8629737609329, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=302, Percent_Identity=30.1324503311258, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=41.5584415584416, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=313, Percent_Identity=26.8370607028754, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=240, Percent_Identity=27.5, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=65.2392947103275, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=453, Percent_Identity=31.1258278145695, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=453, Percent_Identity=31.1258278145695, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=29.9703264094956, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322937, Length=367, Percent_Identity=25.3405994550409, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=291, Percent_Identity=27.1477663230241, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=58.0562659846547, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=58.0562659846547, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=51.421188630491, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=458, Percent_Identity=31.2227074235808, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45553816, Length=458, Percent_Identity=31.2227074235808, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651721, Length=458, Percent_Identity=31.2227074235808, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17864154, Length=458, Percent_Identity=31.2227074235808, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24652838, Length=459, Percent_Identity=31.3725490196078, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17137572, Length=459, Percent_Identity=31.3725490196078, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45550900, Length=439, Percent_Identity=28.9293849658314, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.4093567251462, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=29.8701298701299, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=289, Percent_Identity=27.3356401384083, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647048, Length=289, Percent_Identity=27.3356401384083, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_DESDA (B8J1A0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480205.1
- GeneID:   7285330
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1628
- HOGENOM:   HBG307581
- ProtClustDB:   PRK12735
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43442; Mature: 43311

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEKFERKKPHVNIGTIGHIDHGKTTLTAAITKVANLKSGGKFISYDEIDKAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCHHHCC
ITIATSHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLARQVGVPQLVVFLNKCDLVDDEELLELVELEVRELLSSYDFPGDDVPVIRGSALKALE
HHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHC
SDDPNSAEAKCILDLLDACDSFIPEPVRDIDKPFLMPIEDVFSISGRGTVVTGRVERGVI
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHCCCCCCEEEECCCCCCEE
KVGEEVEIVGIKPTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAAPK
ECCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCC
SITPHKKFKAEVYVLSKEEGGRHTPFFTGYRPQFYFRTTDITGVISLPEGVEMVMPGDNS
CCCCCCCCEEEEEEEECCCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
QFIVELIAPIAMEAGLRFAIREGGRTVGSGVVTEINE
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCC
>Mature Secondary Structure 
GKEKFERKKPHVNIGTIGHIDHGKTTLTAAITKVANLKSGGKFISYDEIDKAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCHHHCC
ITIATSHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLARQVGVPQLVVFLNKCDLVDDEELLELVELEVRELLSSYDFPGDDVPVIRGSALKALE
HHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHC
SDDPNSAEAKCILDLLDACDSFIPEPVRDIDKPFLMPIEDVFSISGRGTVVTGRVERGVI
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHCCCCCCEEEECCCCCCEE
KVGEEVEIVGIKPTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAAPK
ECCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCC
SITPHKKFKAEVYVLSKEEGGRHTPFFTGYRPQFYFRTTDITGVISLPEGVEMVMPGDNS
CCCCCCCCEEEEEEEECCCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
QFIVELIAPIAMEAGLRFAIREGGRTVGSGVVTEINE
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA