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The map label for this gene is hemK [H]

Identifier: 220904892

GI number: 220904892

Start: 1954148

End: 1955065

Strand: Direct

Name: hemK [H]

Synonym: Ddes_1627

Alternate gene names: 220904892

Gene position: 1954148-1955065 (Clockwise)

Preceding gene: 220904891

Following gene: 220904893

Centisome position: 68.01

GC content: 62.96

Gene sequence:

>918_bases
GTGCGTCTGCGTCAATATCTGATTGAAGCTGCGCAAACATTGCAAAAGGCGGGGGTAGACAGCCCCCGCCTTTGCGCGCA
GGTACTGGTGGAAAAAGTGCTGCAGCTTGACCGCCTTGGCTGCGTCATGTACGCCGGGCGACAGTTGAGCGCCCACGAAA
TCCAGACACTGAACACCCTGCTGGCCCGCCGCGCCGCCGGAGAACCGCTGGCCCATATCACGGGCAGCAAAGAATTTTAT
GGGCGCGATTTTGCCGTCACGCCCCACACACTCATCCCGCGGCCTGAAACCGAACTGCTTGTGGATAAAGCCATTGAGGC
GGCGCGAAAAATCATTGACGCTGCGGACAGGCAAGGTTTGCGTCACGCCAAGACAGAAGGCACGCCTGCCGGTTGTGGTC
CCTGTTTTGCTGACCTGGGCTGCGGTTCGGGCTGCATCGGCATTACATTGGCCCTGGAGCTGCCGCATTGGCAGGGCGTA
CTGGTGGACATCAGCCCGGAGGCCGTGCAGACAGCCCGGCACAATGCCGCGAGTCTCGGCGCACAAAACCGCGTATGGTG
CCTGCCCGGGGATATGACCCGGCCGCCTCTGGCGCGGGGCGCATACACCATGCTGGTAAGCAATCCGCCCTATATTGCCG
AATCCGAGCGAAGCATGGTAATGGGAGAAGTGCTGGAGCACGAGCCGCACAGCGCTCTTTTCTCACCACGACAGGGACTC
GCCCACCTCGCAGCCGCCATACAGGCCGCTGCATGGGCGCTTGTCCCCGGTGGCGTGCTGCTGCTGGAGCACGGGGCGGC
ACAGGGCGCGGCAACACGCCGCCTGTTACGTGAGCATGACCTGTTCGAGGCCCCGGTCACCCACAGGGATATGGCCGGGC
TTGAACGCTGTACCGTGGCGTTTCGGGCGCAGGGCTAA

Upstream 100 bases:

>100_bases
TGGTGGCTGCCCGCCCGGCCAAGCCTGCGGCCCCGGCACGACGCCGGAAGGACAAAACCGAGGACGAACTGCCCACCGCA
GAAGCCGGAGCCTGAAAACG

Downstream 100 bases:

>100_bases
CGGCGGTTTCCGGCGGCCAAAAAAACGGCTTCCGCCCCTGATCCTTAAGAATCCTGTTTTTCTTCGAGGCAGAGGTTGTC
CGCAAGCCTGAAAAAACGGC

Product: modification methylase, HemK family

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRLRQYLIEAAQTLQKAGVDSPRLCAQVLVEKVLQLDRLGCVMYAGRQLSAHEIQTLNTLLARRAAGEPLAHITGSKEFY
GRDFAVTPHTLIPRPETELLVDKAIEAARKIIDAADRQGLRHAKTEGTPAGCGPCFADLGCGSGCIGITLALELPHWQGV
LVDISPEAVQTARHNAASLGAQNRVWCLPGDMTRPPLARGAYTMLVSNPPYIAESERSMVMGEVLEHEPHSALFSPRQGL
AHLAAAIQAAAWALVPGGVLLLEHGAAQGAATRRLLREHDLFEAPVTHRDMAGLERCTVAFRAQG

Sequences:

>Translated_305_residues
MRLRQYLIEAAQTLQKAGVDSPRLCAQVLVEKVLQLDRLGCVMYAGRQLSAHEIQTLNTLLARRAAGEPLAHITGSKEFY
GRDFAVTPHTLIPRPETELLVDKAIEAARKIIDAADRQGLRHAKTEGTPAGCGPCFADLGCGSGCIGITLALELPHWQGV
LVDISPEAVQTARHNAASLGAQNRVWCLPGDMTRPPLARGAYTMLVSNPPYIAESERSMVMGEVLEHEPHSALFSPRQGL
AHLAAAIQAAAWALVPGGVLLLEHGAAQGAATRRLLREHDLFEAPVTHRDMAGLERCTVAFRAQG
>Mature_305_residues
MRLRQYLIEAAQTLQKAGVDSPRLCAQVLVEKVLQLDRLGCVMYAGRQLSAHEIQTLNTLLARRAAGEPLAHITGSKEFY
GRDFAVTPHTLIPRPETELLVDKAIEAARKIIDAADRQGLRHAKTEGTPAGCGPCFADLGCGSGCIGITLALELPHWQGV
LVDISPEAVQTARHNAASLGAQNRVWCLPGDMTRPPLARGAYTMLVSNPPYIAESERSMVMGEVLEHEPHSALFSPRQGL
AHLAAAIQAAAWALVPGGVLLLEHGAAQGAATRRLLREHDLFEAPVTHRDMAGLERCTVAFRAQG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=257, Percent_Identity=27.2373540856031, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787463, Length=303, Percent_Identity=36.963696369637, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87082085, Length=260, Percent_Identity=28.4615384615385, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582226, Length=261, Percent_Identity=28.735632183908, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32857; Mature: 32857

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRQYLIEAAQTLQKAGVDSPRLCAQVLVEKVLQLDRLGCVMYAGRQLSAHEIQTLNTL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LARRAAGEPLAHITGSKEFYGRDFAVTPHTLIPRPETELLVDKAIEAARKIIDAADRQGL
HHHHHCCCCHHHHCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
RHAKTEGTPAGCGPCFADLGCGSGCIGITLALELPHWQGVLVDISPEAVQTARHNAASLG
HHHCCCCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHCC
AQNRVWCLPGDMTRPPLARGAYTMLVSNPPYIAESERSMVMGEVLEHEPHSALFSPRQGL
CCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHH
AHLAAAIQAAAWALVPGGVLLLEHGAAQGAATRRLLREHDLFEAPVTHRDMAGLERCTVA
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHHEEE
FRAQG
EEECC
>Mature Secondary Structure
MRLRQYLIEAAQTLQKAGVDSPRLCAQVLVEKVLQLDRLGCVMYAGRQLSAHEIQTLNTL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LARRAAGEPLAHITGSKEFYGRDFAVTPHTLIPRPETELLVDKAIEAARKIIDAADRQGL
HHHHHCCCCHHHHCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
RHAKTEGTPAGCGPCFADLGCGSGCIGITLALELPHWQGVLVDISPEAVQTARHNAASLG
HHHCCCCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHCC
AQNRVWCLPGDMTRPPLARGAYTMLVSNPPYIAESERSMVMGEVLEHEPHSALFSPRQGL
CCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHH
AHLAAAIQAAAWALVPGGVLLLEHGAAQGAATRRLLREHDLFEAPVTHRDMAGLERCTVA
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHHEEE
FRAQG
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]