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The map label for this gene is ribH

Identifier: 220904886

GI number: 220904886

Start: 1945868

End: 1946338

Strand: Direct

Name: ribH

Synonym: Ddes_1621

Alternate gene names: 220904886

Gene position: 1945868-1946338 (Clockwise)

Preceding gene: 220904885

Following gene: 220904887

Centisome position: 67.72

GC content: 59.45

Gene sequence:

>471_bases
ATGAGCACTGTGAAAACCATTGCGGGCCAGTTGGACGCCAAGGGCCTCAAGGTGGCCGTTGTGGCCACCCGCTTTAACGA
CTTTATTGTAGACCGCCTGGTGGGCGGCGCGCTGGACTATCTGGAGCGTCACGGTCTGGACATGGAAAACGTGACCATGG
TGCGCATTCCCGGCGCTTTCGAGATGCCCCTGGTCTGCCAGAAGCTGGCCGCGTCCGGCAAGTATGACGGCATCCTGGCT
CTGGGCGCTGTCATCCGTGGTGGAACTCCCCATTTTGATTACGTATGCGCCGAGGCCAGCAAGGGCATTGCCCAGGCCAT
GCTGCAACACAGCATGCCTGTTGGCTTTGGCCTGCTGACCTGCGACAATATCGAGCAAGCCATTGAGCGGGCCGGTTCCA
AGGCAGGTAACAAGGGTGTGGAAGCTGCGGCTGCCATGCTGGAAACCATCCGCGTTATGGAGCAGTTGTAA

Upstream 100 bases:

>100_bases
GTGAAGTTCGCCAGGCGCAGACACAGCCGCGTTTGCATGACATTTTTATAACCCATATATCACGAAGGAACAGGGCGCAT
CGTTGCGCCGAGGAGTCGCC

Downstream 100 bases:

>100_bases
CCCATGGCCAAAGGCAAAAATGCCACCAGGCGCGGTGAGCGTGAACTGGCTTTTCAGGTTCTGTACGGTCTCTCCTTCAC
GCCGGCCCGCTCTCTTGAAG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSTVKTIAGQLDAKGLKVAVVATRFNDFIVDRLVGGALDYLERHGLDMENVTMVRIPGAFEMPLVCQKLAASGKYDGILA
LGAVIRGGTPHFDYVCAEASKGIAQAMLQHSMPVGFGLLTCDNIEQAIERAGSKAGNKGVEAAAAMLETIRVMEQL

Sequences:

>Translated_156_residues
MSTVKTIAGQLDAKGLKVAVVATRFNDFIVDRLVGGALDYLERHGLDMENVTMVRIPGAFEMPLVCQKLAASGKYDGILA
LGAVIRGGTPHFDYVCAEASKGIAQAMLQHSMPVGFGLLTCDNIEQAIERAGSKAGNKGVEAAAAMLETIRVMEQL
>Mature_155_residues
STVKTIAGQLDAKGLKVAVVATRFNDFIVDRLVGGALDYLERHGLDMENVTMVRIPGAFEMPLVCQKLAASGKYDGILAL
GAVIRGGTPHFDYVCAEASKGIAQAMLQHSMPVGFGLLTCDNIEQAIERAGSKAGNKGVEAAAAMLETIRVMEQL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=53.9007092198582, Blast_Score=157, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324429, Length=146, Percent_Identity=32.1917808219178, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_DESDA (B8J193)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480198.1
- GeneID:   7285320
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1621
- HOGENOM:   HBG311126
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16539; Mature: 16407

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVKTIAGQLDAKGLKVAVVATRFNDFIVDRLVGGALDYLERHGLDMENVTMVRIPGAF
CCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
EMPLVCQKLAASGKYDGILALGAVIRGGTPHFDYVCAEASKGIAQAMLQHSMPVGFGLLT
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CDNIEQAIERAGSKAGNKGVEAAAAMLETIRVMEQL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
STVKTIAGQLDAKGLKVAVVATRFNDFIVDRLVGGALDYLERHGLDMENVTMVRIPGAF
CHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
EMPLVCQKLAASGKYDGILALGAVIRGGTPHFDYVCAEASKGIAQAMLQHSMPVGFGLLT
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
CDNIEQAIERAGSKAGNKGVEAAAAMLETIRVMEQL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA