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The map label for this gene is nusB

Identifier: 220904887

GI number: 220904887

Start: 1946342

End: 1946836

Strand: Direct

Name: nusB

Synonym: Ddes_1622

Alternate gene names: 220904887

Gene position: 1946342-1946836 (Clockwise)

Preceding gene: 220904886

Following gene: 220904888

Centisome position: 67.74

GC content: 57.37

Gene sequence:

>495_bases
ATGGCCAAAGGCAAAAATGCCACCAGGCGCGGTGAGCGTGAACTGGCTTTTCAGGTTCTGTACGGTCTCTCCTTCACGCC
GGCCCGCTCTCTTGAAGAGTTGCGCCGCAGTTTTCGCATGTCGCCTGACAATCTGGCGCGCAGTGAGGAAAGCGGTGTTC
CTGTGGAAGCCTGCGGCTTTTCCTGGGAGCTTGTGGAAGGAGTATGGAGCAACAGCTCTGCTCTGGACGAGACTATCAGC
CGTTTTTCGCACAACTGGCGCGTGGACCGTATGGGCCGGGTTGAACTGACCCTGCTGCGGCTGGCTGCGTATGAGCTTGT
TTTTCGTGCTGATGTACCGCCCAAGGTGGCCATCAATGAAGCTCTGGAGCTGAGTCGCCAGTTTGGTGAAGGCAATGCCA
AGAACTTCATCAACGGCATTCTCGACGCTGTGGCCAAGGCTCTGGAAAACGGCGAGCTGCAACGCTGCGCCGCACCCTCC
AACACATCTATCTGA

Upstream 100 bases:

>100_bases
GCAAGCCATTGAGCGGGCCGGTTCCAAGGCAGGTAACAAGGGTGTGGAAGCTGCGGCTGCCATGCTGGAAACCATCCGCG
TTATGGAGCAGTTGTAACCC

Downstream 100 bases:

>100_bases
ACCGGTATGCCCCATGACGCAAGAACGTTACAATCCGCAAGCAATTGAAGAAAAATGGCAGGCCCGCTGGCAGGCAGAAA
ACGCCTTTGCCTGCAGCCAT

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKGKNATRRGERELAFQVLYGLSFTPARSLEELRRSFRMSPDNLARSEESGVPVEACGFSWELVEGVWSNSSALDETIS
RFSHNWRVDRMGRVELTLLRLAAYELVFRADVPPKVAINEALELSRQFGEGNAKNFINGILDAVAKALENGELQRCAAPS
NTSI

Sequences:

>Translated_164_residues
MAKGKNATRRGERELAFQVLYGLSFTPARSLEELRRSFRMSPDNLARSEESGVPVEACGFSWELVEGVWSNSSALDETIS
RFSHNWRVDRMGRVELTLLRLAAYELVFRADVPPKVAINEALELSRQFGEGNAKNFINGILDAVAKALENGELQRCAAPS
NTSI
>Mature_163_residues
AKGKNATRRGERELAFQVLYGLSFTPARSLEELRRSFRMSPDNLARSEESGVPVEACGFSWELVEGVWSNSSALDETISR
FSHNWRVDRMGRVELTLLRLAAYELVFRADVPPKVAINEALELSRQFGEGNAKNFINGILDAVAKALENGELQRCAAPSN
TSI

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=117, Percent_Identity=36.7521367521368, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_DESDA (B8J194)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480199.1
- GeneID:   7285321
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1622
- HOGENOM:   HBG734409
- ProtClustDB:   CLSK705089
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 18248; Mature: 18117

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKNATRRGERELAFQVLYGLSFTPARSLEELRRSFRMSPDNLARSEESGVPVEACGF
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCC
SWELVEGVWSNSSALDETISRFSHNWRVDRMGRVELTLLRLAAYELVFRADVPPKVAINE
CHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALELSRQFGEGNAKNFINGILDAVAKALENGELQRCAAPSNTSI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
>Mature Secondary Structure 
AKGKNATRRGERELAFQVLYGLSFTPARSLEELRRSFRMSPDNLARSEESGVPVEACGF
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCC
SWELVEGVWSNSSALDETISRFSHNWRVDRMGRVELTLLRLAAYELVFRADVPPKVAINE
CHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALELSRQFGEGNAKNFINGILDAVAKALENGELQRCAAPSNTSI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA