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The map label for this gene is ribE [H]

Identifier: 220904885

GI number: 220904885

Start: 1945000

End: 1945674

Strand: Direct

Name: ribE [H]

Synonym: Ddes_1620

Alternate gene names: 220904885

Gene position: 1945000-1945674 (Clockwise)

Preceding gene: 220904884

Following gene: 220904886

Centisome position: 67.69

GC content: 58.96

Gene sequence:

>675_bases
ATGTTTACCGGTATCATACAGGGACAGGGCGAGGTGCGCTCCATGCGTAAAAGCGGCACGGAATGCCGTATATGTCTGCG
CCCCCTGTTTACCTTGCCCAACATCATTGATGGCGAATCCATTGCCGTCAACGGAGCCTGCCTGTCAGTGGAAGAACACG
GGGCCGACACCTTCACAGCCTATGCCTCGGCCGAGACGCTCACGCGCACAACTCTGGGCGGCCTGCAAGTCGGGCATCTG
GTCAACCTGGAAAGGGCGCTGGCCATGGGCGACCGTCTGGGAGGGCATCTGGTGAGCGGTCACGTGGACTGCCTTGCCAC
CGTGCGCGAAGTACGCCGGACCGGACAGTCCCTTTGCTGCCGCCTTGTCTTTCCCGCCGAATTCGGGGCGGAGGTTATCC
CCAAGGGGTCCGTGTCTCTTGACGGCATAAGTCTTACGGTCAATGATTGCGGCCCGGATTTTCTTGAGGTCAACATCATC
CCCGATACACAGAAGCGCACCACCATGCTGCACTGGCATCCGGGCGTGACGGTCAATATGGAAACCGACCTCATCGGAAA
ATACGTCCGCCGCCTGCTGGGTCCCTGGGCCGGAAACAGTACAAACAGCGACAAGACAGACCCTGAAGTATCGGGGCTGA
GCAAGGATTTTCTGCTGCGCCAAGGTTTTCTATAG

Upstream 100 bases:

>100_bases
CGAAGCCCTGCGCATGCGCGTGACAGGCAGCCACATCAGCGGGGGCGACATCCATATTGAGCTGCGCCCGCTGCAAGCCG
TGGAAGAAAAGTAGGAAATC

Downstream 100 bases:

>100_bases
ACCTTCCATATGGCAATGACCGTGCGCGGCATCGTGCCGCGCGGAAGCGTCATGCCATACTTTTGCGTCATGCGCAGAAA
ACGCCCCTTGCGGGTGAAGT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MFTGIIQGQGEVRSMRKSGTECRICLRPLFTLPNIIDGESIAVNGACLSVEEHGADTFTAYASAETLTRTTLGGLQVGHL
VNLERALAMGDRLGGHLVSGHVDCLATVREVRRTGQSLCCRLVFPAEFGAEVIPKGSVSLDGISLTVNDCGPDFLEVNII
PDTQKRTTMLHWHPGVTVNMETDLIGKYVRRLLGPWAGNSTNSDKTDPEVSGLSKDFLLRQGFL

Sequences:

>Translated_224_residues
MFTGIIQGQGEVRSMRKSGTECRICLRPLFTLPNIIDGESIAVNGACLSVEEHGADTFTAYASAETLTRTTLGGLQVGHL
VNLERALAMGDRLGGHLVSGHVDCLATVREVRRTGQSLCCRLVFPAEFGAEVIPKGSVSLDGISLTVNDCGPDFLEVNII
PDTQKRTTMLHWHPGVTVNMETDLIGKYVRRLLGPWAGNSTNSDKTDPEVSGLSKDFLLRQGFL
>Mature_224_residues
MFTGIIQGQGEVRSMRKSGTECRICLRPLFTLPNIIDGESIAVNGACLSVEEHGADTFTAYASAETLTRTTLGGLQVGHL
VNLERALAMGDRLGGHLVSGHVDCLATVREVRRTGQSLCCRLVFPAEFGAEVIPKGSVSLDGISLTVNDCGPDFLEVNII
PDTQKRTTMLHWHPGVTVNMETDLIGKYVRRLLGPWAGNSTNSDKTDPEVSGLSKDFLLRQGFL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=36.2162162162162, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319733, Length=161, Percent_Identity=36.0248447204969, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24213; Mature: 24213

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIQGQGEVRSMRKSGTECRICLRPLFTLPNIIDGESIAVNGACLSVEEHGADTFTA
CCCEEECCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEECCEEEEEHHCCCCEEEE
YASAETLTRTTLGGLQVGHLVNLERALAMGDRLGGHLVSGHVDCLATVREVRRTGQSLCC
ECCHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHEEE
RLVFPAEFGAEVIPKGSVSLDGISLTVNDCGPDFLEVNIIPDTQKRTTMLHWHPGVTVNM
EEEECHHCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCCEEEEE
ETDLIGKYVRRLLGPWAGNSTNSDKTDPEVSGLSKDFLLRQGFL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MFTGIIQGQGEVRSMRKSGTECRICLRPLFTLPNIIDGESIAVNGACLSVEEHGADTFTA
CCCEEECCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEECCEEEEEHHCCCCEEEE
YASAETLTRTTLGGLQVGHLVNLERALAMGDRLGGHLVSGHVDCLATVREVRRTGQSLCC
ECCHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHEEE
RLVFPAEFGAEVIPKGSVSLDGISLTVNDCGPDFLEVNIIPDTQKRTTMLHWHPGVTVNM
EEEECHHCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCCEEEEE
ETDLIGKYVRRLLGPWAGNSTNSDKTDPEVSGLSKDFLLRQGFL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]