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The map label for this gene is ytsJ [H]

Identifier: 220904802

GI number: 220904802

Start: 1831393

End: 1832664

Strand: Direct

Name: ytsJ [H]

Synonym: Ddes_1534

Alternate gene names: 220904802

Gene position: 1831393-1832664 (Clockwise)

Preceding gene: 220904801

Following gene: 220904805

Centisome position: 63.74

GC content: 56.05

Gene sequence:

>1272_bases
ATGTCTCGTATCAAGAATCTGCGGGATGAAGCCCTGGCCATGCATAAGGAGTATCAGGGCAAAATCGAGGTGCGCGTGAA
AGCGCCCGTGCGCGATACCGACGACCTCACCCTGGCCTACTCGCCCGGCGTGGCCGAGCCTTGCATGGAAATTTACCGCC
AGCCCGAAACACTCGATGTCTATACCAACCACTCCAACTTTGTCTGTGTGGTATCCAACGGCACAGCCGTTCTGGGGCTG
GGCAACATCGGCCCCGCAGCCGCAATGCCGGTTATGGAAGGCAAATCCCTGCTGTTCAAGACTTTCGGCGATGTGGATGC
CTTTCCCATCTGCGTCGATACCAAAGACACGACCAAAATCGTTGAACTGGTGGAACTTATGGCCCCCACCTTCGGCGGCG
TCAACCTTGAGGACATCAAGGCTCCGGAATGCTTCGTCATTGAAGACAGCCTGAAGAAAAACGGCATTTTCAAGGGCCCC
ATCTTTCATGACGACCAGCACGGAACCGCCGTGGTTACCCTATCCGGGCTTATCAACGCGCTCAAGCTGGTAAACAAAAA
ACTTGATGACATCACTGTGGTTACCAGCGGCGCAGGGGCTGCCGGTATTGCCATCATCAAGCTGCTCATGGCTCTTGGCC
TGAAAAATGTTATCATGTGCGACTCGCGCGGGGCCATATGGGATGGCCGCAGCGAAGGAATGAATGCCTATAAGGAAGAA
ATCGCCCGCCGCACCAATCCGCAGAAAATCAAGGGCGGTCTGGCCGAAGCCATCAAGGGGGCAGATGTCTTTATCGGTGT
TTCCGCTCCGGATACACTTAATGAAGATATGATCCGCAGCATGGCCAAAGATCCCATAATTTTTGCTCAGGCCAATCCCA
TCCCCGAAATATGGCCCATCCAGCGCGCCCTGGACGCCGGAGCCAAAGTTGTGGCCACGGGGCGCTCCGACTGCCCCAAC
CAGATCAACAACGTTCTGGCCTTCCCCGGTATTTTCCGTGGCGCTCTTGATGTTGCGGCCACAGATATCAACGATGCCAT
GAAAATTGCGGCGGCATACGCCATCGCCGAGCTGGTCAAACCCGAAGAACTCAGCCCGGGCTACATTATTCCTTCCACGC
TTAACAGCGAGGTGGCTCCCCGGGTGGCGGCGGCCACGGCCAAAGCTGCCATGGAAAGCGGCATTGCCCGCAAACCGATG
GATCCGGAAATCGTGGCGGCAAACCTGAAAAAAAGGCTGGCCGACCGCCAGATTGCGTGCACGAAAAAATAA

Upstream 100 bases:

>100_bases
CTGTTCGCTTCCGCCTGCCCATGCCTGTCATGGGTGCAGCCCCCCGCGCCGTGCGGAACGCGCCGCCCGGAAGCGCACCA
CATCAGTCAAGGAGTGTACA

Downstream 100 bases:

>100_bases
TGTAACGGCCAAAACCTCTTTCTGAACCGGCAGCAAAACAGATGAATCCGGACGAACAAGGTTCTCTCCCGAAGGCAGGC
AGTCCTGCTTTCGGGAGAGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSRIKNLRDEALAMHKEYQGKIEVRVKAPVRDTDDLTLAYSPGVAEPCMEIYRQPETLDVYTNHSNFVCVVSNGTAVLGL
GNIGPAAAMPVMEGKSLLFKTFGDVDAFPICVDTKDTTKIVELVELMAPTFGGVNLEDIKAPECFVIEDSLKKNGIFKGP
IFHDDQHGTAVVTLSGLINALKLVNKKLDDITVVTSGAGAAGIAIIKLLMALGLKNVIMCDSRGAIWDGRSEGMNAYKEE
IARRTNPQKIKGGLAEAIKGADVFIGVSAPDTLNEDMIRSMAKDPIIFAQANPIPEIWPIQRALDAGAKVVATGRSDCPN
QINNVLAFPGIFRGALDVAATDINDAMKIAAAYAIAELVKPEELSPGYIIPSTLNSEVAPRVAAATAKAAMESGIARKPM
DPEIVAANLKKRLADRQIACTKK

Sequences:

>Translated_423_residues
MSRIKNLRDEALAMHKEYQGKIEVRVKAPVRDTDDLTLAYSPGVAEPCMEIYRQPETLDVYTNHSNFVCVVSNGTAVLGL
GNIGPAAAMPVMEGKSLLFKTFGDVDAFPICVDTKDTTKIVELVELMAPTFGGVNLEDIKAPECFVIEDSLKKNGIFKGP
IFHDDQHGTAVVTLSGLINALKLVNKKLDDITVVTSGAGAAGIAIIKLLMALGLKNVIMCDSRGAIWDGRSEGMNAYKEE
IARRTNPQKIKGGLAEAIKGADVFIGVSAPDTLNEDMIRSMAKDPIIFAQANPIPEIWPIQRALDAGAKVVATGRSDCPN
QINNVLAFPGIFRGALDVAATDINDAMKIAAAYAIAELVKPEELSPGYIIPSTLNSEVAPRVAAATAKAAMESGIARKPM
DPEIVAANLKKRLADRQIACTKK
>Mature_422_residues
SRIKNLRDEALAMHKEYQGKIEVRVKAPVRDTDDLTLAYSPGVAEPCMEIYRQPETLDVYTNHSNFVCVVSNGTAVLGLG
NIGPAAAMPVMEGKSLLFKTFGDVDAFPICVDTKDTTKIVELVELMAPTFGGVNLEDIKAPECFVIEDSLKKNGIFKGPI
FHDDQHGTAVVTLSGLINALKLVNKKLDDITVVTSGAGAAGIAIIKLLMALGLKNVIMCDSRGAIWDGRSEGMNAYKEEI
ARRTNPQKIKGGLAEAIKGADVFIGVSAPDTLNEDMIRSMAKDPIIFAQANPIPEIWPIQRALDAGAKVVATGRSDCPNQ
INNVLAFPGIFRGALDVAATDINDAMKIAAAYAIAELVKPEELSPGYIIPSTLNSEVAPRVAAATAKAAMESGIARKPMD
PEIVAANLKKRLADRQIACTKK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=455, Percent_Identity=24.8351648351648, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI62420882, Length=456, Percent_Identity=25.219298245614, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI62420880, Length=456, Percent_Identity=25.219298245614, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI239049447, Length=456, Percent_Identity=25.219298245614, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4505145, Length=456, Percent_Identity=25.8771929824561, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI270265879, Length=373, Percent_Identity=26.2734584450402, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788806, Length=413, Percent_Identity=48.18401937046, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17537199, Length=439, Percent_Identity=23.0068337129841, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=384, Percent_Identity=27.34375, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78707242, Length=401, Percent_Identity=27.431421446384, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI78707238, Length=401, Percent_Identity=27.431421446384, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI78707240, Length=384, Percent_Identity=27.34375, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281362674, Length=458, Percent_Identity=23.7991266375546, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356279, Length=458, Percent_Identity=23.7991266375546, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281362672, Length=458, Percent_Identity=23.7991266375546, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24646388, Length=408, Percent_Identity=27.2058823529412, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646386, Length=408, Percent_Identity=27.2058823529412, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 45256; Mature: 45125

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKNLRDEALAMHKEYQGKIEVRVKAPVRDTDDLTLAYSPGVAEPCMEIYRQPETLDV
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCEEEE
YTNHSNFVCVVSNGTAVLGLGNIGPAAAMPVMEGKSLLFKTFGDVDAFPICVDTKDTTKI
EECCCCEEEEEECCEEEEEECCCCCHHHCCHHCCCHHHHHHCCCCCCEEEEECCCCHHHH
VELVELMAPTFGGVNLEDIKAPECFVIEDSLKKNGIFKGPIFHDDQHGTAVVTLSGLINA
HHHHHHHCCCCCCCCHHHCCCCCEEEEECCHHHCCEEECCCEECCCCCEEEEEHHHHHHH
LKLVNKKLDDITVVTSGAGAAGIAIIKLLMALGLKNVIMCDSRGAIWDGRSEGMNAYKEE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
IARRTNPQKIKGGLAEAIKGADVFIGVSAPDTLNEDMIRSMAKDPIIFAQANPIPEIWPI
HHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHH
QRALDAGAKVVATGRSDCPNQINNVLAFPGIFRGALDVAATDINDAMKIAAAYAIAELVK
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
PEELSPGYIIPSTLNSEVAPRVAAATAKAAMESGIARKPMDPEIVAANLKKRLADRQIAC
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
TKK
CCC
>Mature Secondary Structure 
SRIKNLRDEALAMHKEYQGKIEVRVKAPVRDTDDLTLAYSPGVAEPCMEIYRQPETLDV
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCEEEE
YTNHSNFVCVVSNGTAVLGLGNIGPAAAMPVMEGKSLLFKTFGDVDAFPICVDTKDTTKI
EECCCCEEEEEECCEEEEEECCCCCHHHCCHHCCCHHHHHHCCCCCCEEEEECCCCHHHH
VELVELMAPTFGGVNLEDIKAPECFVIEDSLKKNGIFKGPIFHDDQHGTAVVTLSGLINA
HHHHHHHCCCCCCCCHHHCCCCCEEEEECCHHHCCEEECCCEECCCCCEEEEEHHHHHHH
LKLVNKKLDDITVVTSGAGAAGIAIIKLLMALGLKNVIMCDSRGAIWDGRSEGMNAYKEE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
IARRTNPQKIKGGLAEAIKGADVFIGVSAPDTLNEDMIRSMAKDPIIFAQANPIPEIWPI
HHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHH
QRALDAGAKVVATGRSDCPNQINNVLAFPGIFRGALDVAATDINDAMKIAAAYAIAELVK
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
PEELSPGYIIPSTLNSEVAPRVAAATAKAAMESGIARKPMDPEIVAANLKKRLADRQIAC
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
TKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]