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The map label for this gene is fumA [H]

Identifier: 220904797

GI number: 220904797

Start: 1826479

End: 1827039

Strand: Direct

Name: fumA [H]

Synonym: Ddes_1529

Alternate gene names: 220904797

Gene position: 1826479-1827039 (Clockwise)

Preceding gene: 220904796

Following gene: 220904798

Centisome position: 63.56

GC content: 59.54

Gene sequence:

>561_bases
ATGTCTGACGAAATCAAAAAAATCCGCGCTCCCTTTGATGAAGCCACTGCCCGCTCACTGCGCGCTGGAGACCGTGTTCT
TATTTCCGGCACCATACTGGCAGCGCGTGACGCGGCCCACAAGCGCCTTGTGGAAACCCTGGACAGGGGCGAAGCCCTGC
CTGTGAGTCTTGAAGGCGCGGTGGTCTATTATGTGGGGCCAAGCCCGGCACGCCCCGGACAGCCCATAGGGGCCGCCGGT
CCCACTACCTCGGGCCGCATGGACGCCTATACCCCGCGCCTGCTGGATCAGGGACTTAAAGGCATGATCGGCAAGGGGTA
CCGCAAGCCGGAAGTGGTGGAAGCCATGAAAAAACATGGCGTACCCTATCTTGCGGCCGTGGGAGGGGCTGGAGCGCTCA
TTGCCCGCGCCGTCAAGAAGTATACGGTACTGGCCTATGAAGATCTGGGGCCGGAAGCTGTTGCCGCGATGGAAGTGGAA
GATTTTCCGGCCATTGTGGTGATAGACAGTACCGGTGGCAATTTTTACGAAACGGGGCAGGCTCCGTACAAACGCATATG
A

Upstream 100 bases:

>100_bases
TGTGGAATGGGCGCCGACCCATATCGCCTCTTTGCCGGTCGCCGTAAATATCAACTGCCACGCGGCGCGCCACGCCGAAG
CCGTGCTGTAAGGGGGAGCC

Downstream 100 bases:

>100_bases
AGGAATCACAGTTTTTTCATAATGGCATAGGTACAGAATACTTGTATAAGGAGTTGTCATGGCTTTAAGCAGAACCCCTG
GGGAGGCAAAAACCCGTCTG

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSDEIKKIRAPFDEATARSLRAGDRVLISGTILAARDAAHKRLVETLDRGEALPVSLEGAVVYYVGPSPARPGQPIGAAG
PTTSGRMDAYTPRLLDQGLKGMIGKGYRKPEVVEAMKKHGVPYLAAVGGAGALIARAVKKYTVLAYEDLGPEAVAAMEVE
DFPAIVVIDSTGGNFYETGQAPYKRI

Sequences:

>Translated_186_residues
MSDEIKKIRAPFDEATARSLRAGDRVLISGTILAARDAAHKRLVETLDRGEALPVSLEGAVVYYVGPSPARPGQPIGAAG
PTTSGRMDAYTPRLLDQGLKGMIGKGYRKPEVVEAMKKHGVPYLAAVGGAGALIARAVKKYTVLAYEDLGPEAVAAMEVE
DFPAIVVIDSTGGNFYETGQAPYKRI
>Mature_185_residues
SDEIKKIRAPFDEATARSLRAGDRVLISGTILAARDAAHKRLVETLDRGEALPVSLEGAVVYYVGPSPARPGQPIGAAGP
TTSGRMDAYTPRLLDQGLKGMIGKGYRKPEVVEAMKKHGVPYLAAVGGAGALIARAVKKYTVLAYEDLGPEAVAAMEVED
FPAIVVIDSTGGNFYETGQAPYKRI

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=158, Percent_Identity=41.7721518987342, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1790564, Length=155, Percent_Identity=41.2903225806452, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1789443, Length=175, Percent_Identity=30.8571428571429, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19699; Mature: 19568

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEIKKIRAPFDEATARSLRAGDRVLISGTILAARDAAHKRLVETLDRGEALPVSLEGA
CCCHHHHHHCCHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCEEEECCE
VVYYVGPSPARPGQPIGAAGPTTSGRMDAYTPRLLDQGLKGMIGKGYRKPEVVEAMKKHG
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
VPYLAAVGGAGALIARAVKKYTVLAYEDLGPEAVAAMEVEDFPAIVVIDSTGGNFYETGQ
CCEEEECCCHHHHHHHHHHHHEEEEECCCCCHHEEEEEECCCCEEEEEECCCCCEEECCC
APYKRI
CCCCCC
>Mature Secondary Structure 
SDEIKKIRAPFDEATARSLRAGDRVLISGTILAARDAAHKRLVETLDRGEALPVSLEGA
CCHHHHHHCCHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCEEEECCE
VVYYVGPSPARPGQPIGAAGPTTSGRMDAYTPRLLDQGLKGMIGKGYRKPEVVEAMKKHG
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
VPYLAAVGGAGALIARAVKKYTVLAYEDLGPEAVAAMEVEDFPAIVVIDSTGGNFYETGQ
CCEEEECCCHHHHHHHHHHHHEEEEECCCCCHHEEEEEECCCCEEEEEECCCCCEEECCC
APYKRI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]