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The map label for this gene is pth

Identifier: 220904722

GI number: 220904722

Start: 1742154

End: 1742843

Strand: Direct

Name: pth

Synonym: Ddes_1454

Alternate gene names: 220904722

Gene position: 1742154-1742843 (Clockwise)

Preceding gene: 220904721

Following gene: 220904723

Centisome position: 60.63

GC content: 58.41

Gene sequence:

>690_bases
ATGGACTATAATGGCGTTCTGGTGGGTCTGGGCAATCCGGGCGCACGCTATGAAGGCACACGGCACAACTGCGGCTTCGC
CCTGATAGACGCTTTTGTGGACTTTGGCTACCGTCACGGCACTGTAGACGAAATGAACGGCGGCAAGTTTTCCTGCCAGT
TGTGGCGTGTGCGACTGCCCCGCCTTGACGGCTGCTGGCTGGCCGCCAAGCCGCAGACATTCATGAACCTCAGCGGGCAA
TGCGTACAGCCGCTACTGTCCTGGCATAAGCTCAAAGCAGCAGACCTTGTGGTAGCCCACGATGAACTGGACATACCCCC
CGGTGAACTGCGCTTCAAGTTCGGCGGAGGCAATGCCGGGCACAACGGCTTGAAATCCATTACCGAACTTCTCGGCACAC
CGGATTTTTACCGACTGCGCATGGGCATAGGCCGCCCCCCCCACAAAGGCGATGTGACCAACTGGGTTCTGGGCCGTCCC
CAGGGTGAAGACGCCGAAAACCTGGATCACATCCTGCCACTGGCGCTTGATGTCCTGTTTGCTTTTGCCGACAAAGGCCT
GGACAGCGCCGTGCGCCTGGCTGGAAAAACGACCCGGCCACGCAAACCCGTAAGGCAGACTGCAAACGCTGAAGCCAGCA
ACAACAGCCCGGAAGCTTCCGCAACCCCGCAAAACAAAGATAATACTTAA

Upstream 100 bases:

>100_bases
CTGTGCCGCTACATGGCACGGCACGCAGCGGCAGCCTGCGCAGGACTGGGGCTTCCCTTGCACCTGGAAAAGACATGTCG
CGCTCAGGGAGGCGCAGCGT

Downstream 100 bases:

>100_bases
CCCGGCGTGACGGCGCCAGTGTTTTGTGGTATGTATCCAGTAATACGAAGCGCAGAGGTCTGGTGAATGCCATGTGCACC
CCCCCGTACCTTTGCTCTTG

Product: Aminoacyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MDYNGVLVGLGNPGARYEGTRHNCGFALIDAFVDFGYRHGTVDEMNGGKFSCQLWRVRLPRLDGCWLAAKPQTFMNLSGQ
CVQPLLSWHKLKAADLVVAHDELDIPPGELRFKFGGGNAGHNGLKSITELLGTPDFYRLRMGIGRPPHKGDVTNWVLGRP
QGEDAENLDHILPLALDVLFAFADKGLDSAVRLAGKTTRPRKPVRQTANAEASNNSPEASATPQNKDNT

Sequences:

>Translated_229_residues
MDYNGVLVGLGNPGARYEGTRHNCGFALIDAFVDFGYRHGTVDEMNGGKFSCQLWRVRLPRLDGCWLAAKPQTFMNLSGQ
CVQPLLSWHKLKAADLVVAHDELDIPPGELRFKFGGGNAGHNGLKSITELLGTPDFYRLRMGIGRPPHKGDVTNWVLGRP
QGEDAENLDHILPLALDVLFAFADKGLDSAVRLAGKTTRPRKPVRQTANAEASNNSPEASATPQNKDNT
>Mature_229_residues
MDYNGVLVGLGNPGARYEGTRHNCGFALIDAFVDFGYRHGTVDEMNGGKFSCQLWRVRLPRLDGCWLAAKPQTFMNLSGQ
CVQPLLSWHKLKAADLVVAHDELDIPPGELRFKFGGGNAGHNGLKSITELLGTPDFYRLRMGIGRPPHKGDVTNWVLGRP
QGEDAENLDHILPLALDVLFAFADKGLDSAVRLAGKTTRPRKPVRQTANAEASNNSPEASATPQNKDNT

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=175, Percent_Identity=33.7142857142857, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1787455, Length=168, Percent_Identity=43.452380952381, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321983, Length=173, Percent_Identity=31.2138728323699, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_DESDA (B8J0S9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480034.1
- GeneID:   7285151
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1454
- HOGENOM:   HBG610927
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 25003; Mature: 25003

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYNGVLVGLGNPGARYEGTRHNCGFALIDAFVDFGYRHGTVDEMNGGKFSCQLWRVRLP
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECC
RLDGCWLAAKPQTFMNLSGQCVQPLLSWHKLKAADLVVAHDELDIPPGELRFKFGGGNAG
CCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCC
HNGLKSITELLGTPDFYRLRMGIGRPPHKGDVTNWVLGRPQGEDAENLDHILPLALDVLF
CHHHHHHHHHHCCCCCEEHEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
AFADKGLDSAVRLAGKTTRPRKPVRQTANAEASNNSPEASATPQNKDNT
HHHHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDYNGVLVGLGNPGARYEGTRHNCGFALIDAFVDFGYRHGTVDEMNGGKFSCQLWRVRLP
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECC
RLDGCWLAAKPQTFMNLSGQCVQPLLSWHKLKAADLVVAHDELDIPPGELRFKFGGGNAG
CCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCC
HNGLKSITELLGTPDFYRLRMGIGRPPHKGDVTNWVLGRPQGEDAENLDHILPLALDVLF
CHHHHHHHHHHCCCCCEEHEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
AFADKGLDSAVRLAGKTTRPRKPVRQTANAEASNNSPEASATPQNKDNT
HHHHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA