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The map label for this gene is murD

Identifier: 220904382

GI number: 220904382

Start: 1304464

End: 1305768

Strand: Direct

Name: murD

Synonym: Ddes_1111

Alternate gene names: 220904382

Gene position: 1304464-1305768 (Clockwise)

Preceding gene: 220904381

Following gene: 220904383

Centisome position: 45.4

GC content: 56.93

Gene sequence:

>1305_bases
ATGGCATTGGAAAAAACGCGCGGCAGACGCATTAGCGTTGGCGAAACGGCAGTGGTGGTGGGGGCGGGGCGTTCCGGCCT
TGCCGCTGCGCGGCTCTTGTGTCGTGAAGGAGCGCAGGTGCGCCTGCTGGACAGCAACGCTGATGCCTTTTCCGGCAGGG
AAGCACTGGCGGGCGAGCTGCGGCAACTCGGCATCAGTATAGAACTTGGCCCCCACAAGCCTGACCAGTTTGAAAATGCC
GCCTTTGTCGTACCAAGCCCCGGCATGCCTGTGGCGCGCCTTGCGGGCCTTGTGGATGAAGAGCGTGCGGAAATTCTGGC
AGAAATGGAACTGGCATGGCGGTATCTGGAAAACGAGCCTGTGCTGGCCGTTACCGGAACCAGCGGCAAGACCACAACTG
CATCGCTGGCGGCGGCCATGCTGCATGAGCAGGGCTATGCCGTTTTTCTGGGCGGCAACATCGGCACCCCCCTGTCGGAA
TACGTACTTTCCGGACACAAGGCGGATGTGCTCGTGCTGGAGATTTCCAGCTTCCAGCTGCAAACTTGTTCTACGTTTTG
CCCACGGGCCGGCATTTTACTTAACATTACTCCCAATCATCTGGATTATCACAAAGATATGGCCGAATACACGGAGGCAA
AGTTTCGCCTGTTCCGATGCCAGGACGAAGGCGATCTTGCCGTACTCGGCGAAAGCCTGCGCAGTCTTGCGGCCCGGTAC
GGTCTGAAGGCCCGCCAAGTTTATGTAAGCGATGCGGGCCGCTTTTCCGGCAGTTCCCTCATGGGCGCACATAATCGCGT
AAATGAAGAAGCGGCCTGGCAGGCCTGCCGTCTTTTTGGCGTCAGCGAAGAAAATGCAGCCAGAGCCTTGGCGCGTTTTG
CTCCCCTGCCGCACCGTCTTGAACGGGTACGTGAACTTGAAGGTGTTCTTTTTGTCAATGATTCCAAATGTACAACGGTT
TCATCACTTAAAGTGGCCCTTGAAGCTTTTGATCGCCCCGTGCGTCTTGTGTGTGGTGGAAAATTCAAGGGTGGCGATCT
TGCCGGTCTGGCGGATCTCGTTAAAAACCGTGTAAGCGCAGTAGCTCTTTTCGGGGCCGGCAGGGAGCATTTTGAACGGG
CATGGCAGGGGCTTGTTCCCATGACCTGGCATGCCTCTCTTGAACCTGCCGTAAAGCATCTCGCCGCCTCGGCGTGCAGG
GGCGACGTGGTGCTTATGGCCCCGGCCACTTCAAGTTTTGATCTTTACGCCAACTATGAAGAACGTGGAAAGGACTTCAA
GCGTATAGTGGGTAAGCTGTCATGA

Upstream 100 bases:

>100_bases
GCGTGCCAGAGTCCAAAATCATCATCCGCTTCTGGATCACGTCAATTCTGCTTGGCCTCATGGCGCTTTCAGTCCTGAAG
CTGCGCTGAGGAGATATGGT

Downstream 100 bases:

>100_bases
AAAACGTTTTTGCCGCAAAGGCCAAAAACAATAAGGCGCACAGCGCCATGGGCCGGGCTACGGAAAAAGGCCCTTTTGCG
CCTTTTGACTGGTGGCTGTT

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MALEKTRGRRISVGETAVVVGAGRSGLAAARLLCREGAQVRLLDSNADAFSGREALAGELRQLGISIELGPHKPDQFENA
AFVVPSPGMPVARLAGLVDEERAEILAEMELAWRYLENEPVLAVTGTSGKTTTASLAAAMLHEQGYAVFLGGNIGTPLSE
YVLSGHKADVLVLEISSFQLQTCSTFCPRAGILLNITPNHLDYHKDMAEYTEAKFRLFRCQDEGDLAVLGESLRSLAARY
GLKARQVYVSDAGRFSGSSLMGAHNRVNEEAAWQACRLFGVSEENAARALARFAPLPHRLERVRELEGVLFVNDSKCTTV
SSLKVALEAFDRPVRLVCGGKFKGGDLAGLADLVKNRVSAVALFGAGREHFERAWQGLVPMTWHASLEPAVKHLAASACR
GDVVLMAPATSSFDLYANYEERGKDFKRIVGKLS

Sequences:

>Translated_434_residues
MALEKTRGRRISVGETAVVVGAGRSGLAAARLLCREGAQVRLLDSNADAFSGREALAGELRQLGISIELGPHKPDQFENA
AFVVPSPGMPVARLAGLVDEERAEILAEMELAWRYLENEPVLAVTGTSGKTTTASLAAAMLHEQGYAVFLGGNIGTPLSE
YVLSGHKADVLVLEISSFQLQTCSTFCPRAGILLNITPNHLDYHKDMAEYTEAKFRLFRCQDEGDLAVLGESLRSLAARY
GLKARQVYVSDAGRFSGSSLMGAHNRVNEEAAWQACRLFGVSEENAARALARFAPLPHRLERVRELEGVLFVNDSKCTTV
SSLKVALEAFDRPVRLVCGGKFKGGDLAGLADLVKNRVSAVALFGAGREHFERAWQGLVPMTWHASLEPAVKHLAASACR
GDVVLMAPATSSFDLYANYEERGKDFKRIVGKLS
>Mature_433_residues
ALEKTRGRRISVGETAVVVGAGRSGLAAARLLCREGAQVRLLDSNADAFSGREALAGELRQLGISIELGPHKPDQFENAA
FVVPSPGMPVARLAGLVDEERAEILAEMELAWRYLENEPVLAVTGTSGKTTTASLAAAMLHEQGYAVFLGGNIGTPLSEY
VLSGHKADVLVLEISSFQLQTCSTFCPRAGILLNITPNHLDYHKDMAEYTEAKFRLFRCQDEGDLAVLGESLRSLAARYG
LKARQVYVSDAGRFSGSSLMGAHNRVNEEAAWQACRLFGVSEENAARALARFAPLPHRLERVRELEGVLFVNDSKCTTVS
SLKVALEAFDRPVRLVCGGKFKGGDLAGLADLVKNRVSAVALFGAGREHFERAWQGLVPMTWHASLEPAVKHLAASACRG
DVVLMAPATSSFDLYANYEERGKDFKRIVGKLS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=446, Percent_Identity=30.2690582959641, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_DESDA (B8IZT9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479694.1
- GeneID:   7284793
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1111
- HOGENOM:   HBG750024
- ProtClustDB:   CLSK705829
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR002938
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF01494 FAD_binding_3; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46918; Mature: 46787

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEKTRGRRISVGETAVVVGAGRSGLAAARLLCREGAQVRLLDSNADAFSGREALAGEL
CCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHH
RQLGISIELGPHKPDQFENAAFVVPSPGMPVARLAGLVDEERAEILAEMELAWRYLENEP
HHCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VLAVTGTSGKTTTASLAAAMLHEQGYAVFLGGNIGTPLSEYVLSGHKADVLVLEISSFQL
EEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEECCCEE
QTCSTFCPRAGILLNITPNHLDYHKDMAEYTEAKFRLFRCQDEGDLAVLGESLRSLAARY
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
GLKARQVYVSDAGRFSGSSLMGAHNRVNEEAAWQACRLFGVSEENAARALARFAPLPHRL
CCCEEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
ERVRELEGVLFVNDSKCTTVSSLKVALEAFDRPVRLVCGGKFKGGDLAGLADLVKNRVSA
HHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHE
VALFGAGREHFERAWQGLVPMTWHASLEPAVKHLAASACRGDVVLMAPATSSFDLYANYE
EEEECCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECHH
ERGKDFKRIVGKLS
HHCHHHHHHHHCCC
>Mature Secondary Structure 
ALEKTRGRRISVGETAVVVGAGRSGLAAARLLCREGAQVRLLDSNADAFSGREALAGEL
CCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHH
RQLGISIELGPHKPDQFENAAFVVPSPGMPVARLAGLVDEERAEILAEMELAWRYLENEP
HHCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VLAVTGTSGKTTTASLAAAMLHEQGYAVFLGGNIGTPLSEYVLSGHKADVLVLEISSFQL
EEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEECCCEE
QTCSTFCPRAGILLNITPNHLDYHKDMAEYTEAKFRLFRCQDEGDLAVLGESLRSLAARY
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
GLKARQVYVSDAGRFSGSSLMGAHNRVNEEAAWQACRLFGVSEENAARALARFAPLPHRL
CCCEEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
ERVRELEGVLFVNDSKCTTVSSLKVALEAFDRPVRLVCGGKFKGGDLAGLADLVKNRVSA
HHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHE
VALFGAGREHFERAWQGLVPMTWHASLEPAVKHLAASACRGDVVLMAPATSSFDLYANYE
EEEECCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECHH
ERGKDFKRIVGKLS
HHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA