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The map label for this gene is pyrG

Identifier: 220904248

GI number: 220904248

Start: 1135941

End: 1137584

Strand: Reverse

Name: pyrG

Synonym: Ddes_0975

Alternate gene names: 220904248

Gene position: 1137584-1135941 (Counterclockwise)

Preceding gene: 220904250

Following gene: 220904247

Centisome position: 39.59

GC content: 56.45

Gene sequence:

>1644_bases
ATGAAAACTAAATTTATCTTTGTGACGGGCGGAGTACTGTCATCCTTGGGCAAGGGCCTGGCGGCCGCCTCGCTGGGCGC
GCTGCTGCAAACCCGTGGCCTTTCGGTAACAATACAGAAACTCGACCCCTATATTAACGTTGACCCCGGCACTATGAATC
CTTTCCAGCATGGTGAGGTTTTCGTCACTGATGACGGGGCTGAAACCGATCTGGACCTGGGCCATTACGAGCGCTATCTC
AATGTGCCCATGTCGCGGAAAAACAATACCACGTCAGGGGCCATCTATAACCAGGTTATCGCCAAGGAACGTCACGGTGA
CTACCTGGGAGCCACGGTACAGGTCATCCCCCATATTACGGATGAAATCAAAAGCGTGGTTCTCTCCCTGGCCGAAGGCG
AAGACGCCCCGGACGTCGCCATCATTGAAATAGGCGGCACCGTGGGCGATATTGAAGGCCTGCCCTTTCTTGAGGCCATA
CGCCAGCTGCGCTCCGAACTTGGGCGCGACAACTGCCTGAACATCCATCTTACCCTGGTTCCCTACCTGCGCAGCGCCGG
CGAGCACAAGACCAAGCCCACCCAGCACAGCGTAAAGGAACTGCTCTCCATCGGCATTCAGCCCGATATCATCCTCTGCC
GCTGTGAACAGAGCATTCCTGAAGAACTGCGCCGCAAGATCGCCCTGTTCTGCAATGTGGATCAGGATGCCGTGTTCTCT
TCGGTAGACGTAAACAATATCTATGAAGTGCCGCTCAAGTTTTATGCGGAAGGCTTTGATCAGAAAGTGGCCATCATGCT
GCGCCTGCCCGCACGCAATGCCCAGCTTGATGCGTGGGAAAAACTCGTCAGCGACAGCGACAATCCTCACGGCAAGGTCA
CGGTCGCCATCGTGGGCAAGTATGTAGACCTGAAAGAGGCCTACAAAAGCCTGCACGAAGCCCTCATCCACGGCGGCGTG
GCCAACCGGGTTCAGGTGGACCTGCGCTACGTCAACTCTGAGAATGTGGACGACAGCAACGCTGCCGAACACTTCAAAGG
CTGTGACGGCATTCTGGTGCCCGGCGGTTTCGGCTATCGCGGCGTGGAAGGCAAGATCGCGGCCATCCGCTACGCCCGTG
AAAACAAGGTACCGTTCTTCGGCATCTGCCTCGGCATGCAGTGCGCAGTTATCGAATTTGCCCGCCACATGGCGGACATG
GCCGATGCCAACTCCGAAGAGTTTGACCACCGCTCCAAGCACAAGGTCATCTATCTCATGACTGAATGGTATGACTTCCG
CACCAGAAACGTCGAAAAACGCGATGCCGGCAGCGACAAGGGCGGCACCATGCGCCTTGGCTCCTATCCCTGCAAGGTCA
TGCCCGAATCGCGCGCCTTTGAAGCGTACAAGACCGACATGGTTGAAGAACGCCATCGCCACCGTTACGAATTCAACAAT
GAATTCAAGGAAGCCCTGGCGGAAAAAGGCATGATATTCAGCGGCACTTCGCCCGATGGCTCCCTGATGGAGATCATCGA
ACTTCCGGAACATCCCTGGTTCCTGGGCTGCCAGTTCCACCCCGAGTTCAAGTCCCGCCCCATGAATGCCCATCCGCTGT
TCCGGGAATTCATCGGCGCGGCTAAAAAGCACGCCAAGGTCTGA

Upstream 100 bases:

>100_bases
ACGTACCTTGTTTTCGATAGTCAATGCTAGCCAAAAAAATCATTTTGGTCTATGAACTTGGGGTTGTGCTAAAAAGCGAT
CAAGAATCAAGGTGGAGGCC

Downstream 100 bases:

>100_bases
TCCCCGGCGTGAGGGGCTTTCCTCTTTGCACACCTTCGCGCGGGCCTCTGGCCCGCGCTTTTATTTGCAGGCTTGTCTGA
ACGCACAGCAACATGTTTGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MKTKFIFVTGGVLSSLGKGLAAASLGALLQTRGLSVTIQKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERYL
NVPMSRKNNTTSGAIYNQVIAKERHGDYLGATVQVIPHITDEIKSVVLSLAEGEDAPDVAIIEIGGTVGDIEGLPFLEAI
RQLRSELGRDNCLNIHLTLVPYLRSAGEHKTKPTQHSVKELLSIGIQPDIILCRCEQSIPEELRRKIALFCNVDQDAVFS
SVDVNNIYEVPLKFYAEGFDQKVAIMLRLPARNAQLDAWEKLVSDSDNPHGKVTVAIVGKYVDLKEAYKSLHEALIHGGV
ANRVQVDLRYVNSENVDDSNAAEHFKGCDGILVPGGFGYRGVEGKIAAIRYARENKVPFFGICLGMQCAVIEFARHMADM
ADANSEEFDHRSKHKVIYLMTEWYDFRTRNVEKRDAGSDKGGTMRLGSYPCKVMPESRAFEAYKTDMVEERHRHRYEFNN
EFKEALAEKGMIFSGTSPDGSLMEIIELPEHPWFLGCQFHPEFKSRPMNAHPLFREFIGAAKKHAKV

Sequences:

>Translated_547_residues
MKTKFIFVTGGVLSSLGKGLAAASLGALLQTRGLSVTIQKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERYL
NVPMSRKNNTTSGAIYNQVIAKERHGDYLGATVQVIPHITDEIKSVVLSLAEGEDAPDVAIIEIGGTVGDIEGLPFLEAI
RQLRSELGRDNCLNIHLTLVPYLRSAGEHKTKPTQHSVKELLSIGIQPDIILCRCEQSIPEELRRKIALFCNVDQDAVFS
SVDVNNIYEVPLKFYAEGFDQKVAIMLRLPARNAQLDAWEKLVSDSDNPHGKVTVAIVGKYVDLKEAYKSLHEALIHGGV
ANRVQVDLRYVNSENVDDSNAAEHFKGCDGILVPGGFGYRGVEGKIAAIRYARENKVPFFGICLGMQCAVIEFARHMADM
ADANSEEFDHRSKHKVIYLMTEWYDFRTRNVEKRDAGSDKGGTMRLGSYPCKVMPESRAFEAYKTDMVEERHRHRYEFNN
EFKEALAEKGMIFSGTSPDGSLMEIIELPEHPWFLGCQFHPEFKSRPMNAHPLFREFIGAAKKHAKV
>Mature_547_residues
MKTKFIFVTGGVLSSLGKGLAAASLGALLQTRGLSVTIQKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERYL
NVPMSRKNNTTSGAIYNQVIAKERHGDYLGATVQVIPHITDEIKSVVLSLAEGEDAPDVAIIEIGGTVGDIEGLPFLEAI
RQLRSELGRDNCLNIHLTLVPYLRSAGEHKTKPTQHSVKELLSIGIQPDIILCRCEQSIPEELRRKIALFCNVDQDAVFS
SVDVNNIYEVPLKFYAEGFDQKVAIMLRLPARNAQLDAWEKLVSDSDNPHGKVTVAIVGKYVDLKEAYKSLHEALIHGGV
ANRVQVDLRYVNSENVDDSNAAEHFKGCDGILVPGGFGYRGVEGKIAAIRYARENKVPFFGICLGMQCAVIEFARHMADM
ADANSEEFDHRSKHKVIYLMTEWYDFRTRNVEKRDAGSDKGGTMRLGSYPCKVMPESRAFEAYKTDMVEERHRHRYEFNN
EFKEALAEKGMIFSGTSPDGSLMEIIELPEHPWFLGCQFHPEFKSRPMNAHPLFREFIGAAKKHAKV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=44.2831215970962, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=558, Percent_Identity=43.010752688172, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=558, Percent_Identity=43.010752688172, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=558, Percent_Identity=43.010752688172, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=546, Percent_Identity=57.3260073260073, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=616, Percent_Identity=38.3116883116883, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=574, Percent_Identity=42.3344947735192, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=41.4762741652021, Blast_Score=438, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=44.5438282647585, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=43.0555555555556, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_DESDA (B8IZF5)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479560.1
- ProteinModelPortal:   B8IZF5
- GeneID:   7284655
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0975
- HOGENOM:   HBG597806
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61063; Mature: 61063

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 383-383 ACT_SITE 520-520 ACT_SITE 522-522

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKFIFVTGGVLSSLGKGLAAASLGALLQTRGLSVTIQKLDPYINVDPGTMNPFQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEE
FVTDDGAETDLDLGHYERYLNVPMSRKNNTTSGAIYNQVIAKERHGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHH
DEIKSVVLSLAEGEDAPDVAIIEIGGTVGDIEGLPFLEAIRQLRSELGRDNCLNIHLTLV
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PYLRSAGEHKTKPTQHSVKELLSIGIQPDIILCRCEQSIPEELRRKIALFCNVDQDAVFS
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEEECCCHHHHCC
SVDVNNIYEVPLKFYAEGFDQKVAIMLRLPARNAQLDAWEKLVSDSDNPHGKVTVAIVGK
CCCCCCHHHCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEEEC
YVDLKEAYKSLHEALIHGGVANRVQVDLRYVNSENVDDSNAAEHFKGCDGILVPGGFGYR
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCCEEECCCCCCC
GVEGKIAAIRYARENKVPFFGICLGMQCAVIEFARHMADMADANSEEFDHRSKHKVIYLM
CCCCCEEEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEE
TEWYDFRTRNVEKRDAGSDKGGTMRLGSYPCKVMPESRAFEAYKTDMVEERHRHRYEFNN
ECCCCHHCCCCCHHCCCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCH
EFKEALAEKGMIFSGTSPDGSLMEIIELPEHPWFLGCQFHPEFKSRPMNAHPLFREFIGA
HHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHH
AKKHAKV
HHHHCCC
>Mature Secondary Structure
MKTKFIFVTGGVLSSLGKGLAAASLGALLQTRGLSVTIQKLDPYINVDPGTMNPFQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEE
FVTDDGAETDLDLGHYERYLNVPMSRKNNTTSGAIYNQVIAKERHGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHH
DEIKSVVLSLAEGEDAPDVAIIEIGGTVGDIEGLPFLEAIRQLRSELGRDNCLNIHLTLV
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PYLRSAGEHKTKPTQHSVKELLSIGIQPDIILCRCEQSIPEELRRKIALFCNVDQDAVFS
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEEECCCHHHHCC
SVDVNNIYEVPLKFYAEGFDQKVAIMLRLPARNAQLDAWEKLVSDSDNPHGKVTVAIVGK
CCCCCCHHHCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEEEC
YVDLKEAYKSLHEALIHGGVANRVQVDLRYVNSENVDDSNAAEHFKGCDGILVPGGFGYR
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCCEEECCCCCCC
GVEGKIAAIRYARENKVPFFGICLGMQCAVIEFARHMADMADANSEEFDHRSKHKVIYLM
CCCCCEEEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEE
TEWYDFRTRNVEKRDAGSDKGGTMRLGSYPCKVMPESRAFEAYKTDMVEERHRHRYEFNN
ECCCCHHCCCCCHHCCCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCH
EFKEALAEKGMIFSGTSPDGSLMEIIELPEHPWFLGCQFHPEFKSRPMNAHPLFREFIGA
HHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHH
AKKHAKV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA