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The map label for this gene is 220904250

Identifier: 220904250

GI number: 220904250

Start: 1138901

End: 1140043

Strand: Reverse

Name: 220904250

Synonym: Ddes_0977

Alternate gene names: NA

Gene position: 1140043-1138901 (Counterclockwise)

Preceding gene: 220904251

Following gene: 220904248

Centisome position: 39.68

GC content: 55.47

Gene sequence:

>1143_bases
ATGTCTTTTCCCGCACTTAAAATAGGTAATCTCACCGCCAAGATCCCTGTAGTCCAGGGTGGCATGGGTGTAGGCATATC
ACTTTCAGGCCTGGCTTCGGCCGTGGCCAATCAGGGCGGCATCGGCGTTATCGCCGGAGCCATGATCGGCATGAAAGAAC
CGGACGTGGCCAAGGATCCCTTGACCGCCAACCTGCGGGCGCTGCGCAACGAAATCGAAAAAGCCCGCCAGATGACCAGC
GGCATCGTGGGCGTCAACCTTATGGTGGCGCTGACCACCTTCAGCCAGATGGTGCGCACCACCATTGAAAGCAAGGCAGA
CATCATCTTTTCCGGTGCAGGGCTGCCCCTGGATATGCCCAAGCACCTTTTTCAGGCCTGCGAAGAAAAGAAAGAAGAAT
TTAAAACCAAGCTGGTACCCATAGTGTCTTCGGCCCGGGCAGCGTCGGTTATTGCCAAAAAATGGATATCCCGCTTCAAC
TATGTGCCCGATGCCTTTGTGGTTGAAGGCCCCAAGGCAGGCGGGCACCTTGGCTTCAAGGCCGAAGAGCTGCACGATCC
GGCTCACTCCCTTGAGGTGCTGGTTCCTGAGGTGGTAGAAGCCGTGAAGTCTTTTGAAGACAAATACGGCAAGGCCGTTC
CCGTTATCGCTGCCGGCGGCGTGTACTCCGGCGCGGACATCAAAAAATTTCTTGACCTCGGAGCCTCGGGCGTACAGATG
GGAACCCGCTTTGTGGCTACCCACGAATGCGATGCCGACGAACGCTTCAAACAGTCCTACCTGGCAGCCAGGGCTGAAGA
CATCACCATTATCAAAAGCCCGGTGGGCATGCCCGGCCGCGCCCTGGACAATGGGTTCATCCATGCCGCCCGCGAAGGCA
ACAAAAAGCCCTTCAAATGCATCTTCCACTGCGTACACACCTGCGAACAGGAAAAAACCCCCTACTGCATTGCCCAGGCG
CTCATTAATGCCATGAAGGGCAACCTTGAGCGCGGCTTTGCCTTTTGTGGCGCCAACGTGTCGCGTGTAAACAAGATCAC
GTCTGTACGCGAACTTATGGACAGCCTACAGCATGAATTTGACGAAGCCATGTCTTCGCTGAGCAAGAGTGTGCAAAGCA
TGTTCAACAACGGCAAAAGTTAA

Upstream 100 bases:

>100_bases
TACGGCTATTGTTGCGCCTAATTTCCATACGGGGCATGCCTGAGAACCACCAACGGGGGCGGGTGGGCTTGTCCTTACAA
GTCAGGACGGAGGTCTTGCA

Downstream 100 bases:

>100_bases
AAAAACAAATCCTGCGTGGTCGCAAGCAAAGGCGCCACAGCCATACAAACAAAATCCCCTGGCAGAGCCAGGGGATTTTG
TTTGTATGATGTCTGCAACA

Product: 2-nitropropane dioxygenase NPD

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSFPALKIGNLTAKIPVVQGGMGVGISLSGLASAVANQGGIGVIAGAMIGMKEPDVAKDPLTANLRALRNEIEKARQMTS
GIVGVNLMVALTTFSQMVRTTIESKADIIFSGAGLPLDMPKHLFQACEEKKEEFKTKLVPIVSSARAASVIAKKWISRFN
YVPDAFVVEGPKAGGHLGFKAEELHDPAHSLEVLVPEVVEAVKSFEDKYGKAVPVIAAGGVYSGADIKKFLDLGASGVQM
GTRFVATHECDADERFKQSYLAARAEDITIIKSPVGMPGRALDNGFIHAAREGNKKPFKCIFHCVHTCEQEKTPYCIAQA
LINAMKGNLERGFAFCGANVSRVNKITSVRELMDSLQHEFDEAMSSLSKSVQSMFNNGKS

Sequences:

>Translated_380_residues
MSFPALKIGNLTAKIPVVQGGMGVGISLSGLASAVANQGGIGVIAGAMIGMKEPDVAKDPLTANLRALRNEIEKARQMTS
GIVGVNLMVALTTFSQMVRTTIESKADIIFSGAGLPLDMPKHLFQACEEKKEEFKTKLVPIVSSARAASVIAKKWISRFN
YVPDAFVVEGPKAGGHLGFKAEELHDPAHSLEVLVPEVVEAVKSFEDKYGKAVPVIAAGGVYSGADIKKFLDLGASGVQM
GTRFVATHECDADERFKQSYLAARAEDITIIKSPVGMPGRALDNGFIHAAREGNKKPFKCIFHCVHTCEQEKTPYCIAQA
LINAMKGNLERGFAFCGANVSRVNKITSVRELMDSLQHEFDEAMSSLSKSVQSMFNNGKS
>Mature_379_residues
SFPALKIGNLTAKIPVVQGGMGVGISLSGLASAVANQGGIGVIAGAMIGMKEPDVAKDPLTANLRALRNEIEKARQMTSG
IVGVNLMVALTTFSQMVRTTIESKADIIFSGAGLPLDMPKHLFQACEEKKEEFKTKLVPIVSSARAASVIAKKWISRFNY
VPDAFVVEGPKAGGHLGFKAEELHDPAHSLEVLVPEVVEAVKSFEDKYGKAVPVIAAGGVYSGADIKKFLDLGASGVQMG
TRFVATHECDADERFKQSYLAARAEDITIIKSPVGMPGRALDNGFIHAAREGNKKPFKCIFHCVHTCEQEKTPYCIAQAL
INAMKGNLERGFAFCGANVSRVNKITSVRELMDSLQHEFDEAMSSLSKSVQSMFNNGKS

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 40750; Mature: 40618

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPALKIGNLTAKIPVVQGGMGVGISLSGLASAVANQGGIGVIAGAMIGMKEPDVAKDP
CCCCCEEECCEEEECEEEECCCCCCEEHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCC
LTANLRALRNEIEKARQMTSGIVGVNLMVALTTFSQMVRTTIESKADIIFSGAGLPLDMP
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCH
KHLFQACEEKKEEFKTKLVPIVSSARAASVIAKKWISRFNYVPDAFVVEGPKAGGHLGFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
AEELHDPAHSLEVLVPEVVEAVKSFEDKYGKAVPVIAAGGVYSGADIKKFLDLGASGVQM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHH
GTRFVATHECDADERFKQSYLAARAEDITIIKSPVGMPGRALDNGFIHAAREGNKKPFKC
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCEECCCCCCCCHHHH
IFHCVHTCEQEKTPYCIAQALINAMKGNLERGFAFCGANVSRVNKITSVRELMDSLQHEF
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DEAMSSLSKSVQSMFNNGKS
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SFPALKIGNLTAKIPVVQGGMGVGISLSGLASAVANQGGIGVIAGAMIGMKEPDVAKDP
CCCCEEECCEEEECEEEECCCCCCEEHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCC
LTANLRALRNEIEKARQMTSGIVGVNLMVALTTFSQMVRTTIESKADIIFSGAGLPLDMP
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCH
KHLFQACEEKKEEFKTKLVPIVSSARAASVIAKKWISRFNYVPDAFVVEGPKAGGHLGFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
AEELHDPAHSLEVLVPEVVEAVKSFEDKYGKAVPVIAAGGVYSGADIKKFLDLGASGVQM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHH
GTRFVATHECDADERFKQSYLAARAEDITIIKSPVGMPGRALDNGFIHAAREGNKKPFKC
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCEECCCCCCCCHHHH
IFHCVHTCEQEKTPYCIAQALINAMKGNLERGFAFCGANVSRVNKITSVRELMDSLQHEF
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DEAMSSLSKSVQSMFNNGKS
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA