Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 220904247

GI number: 220904247

Start: 1134210

End: 1135634

Strand: Reverse

Name: rpoN [H]

Synonym: Ddes_0974

Alternate gene names: 220904247

Gene position: 1135634-1134210 (Counterclockwise)

Preceding gene: 220904248

Following gene: 220904246

Centisome position: 39.52

GC content: 55.02

Gene sequence:

>1425_bases
ATGGCACTGGAACTTCGACAGCAATTAAAACTGGCTCAGCAGTTGGTGATGACCCCGCAATTACAGCAGGCCATCAAACT
GCTTCAGCTCTCGCGTCTGGAGCTTCTGGAAACCGTGCAGCAGGAACTGCTGGAAAACCCCTTTCTTGAGGAATCCCACG
GAGACGACGCCGCACAGGAACACGTGGAAGACCGGCGGGAAAGCGGTGCTGAGGAAGCATACGACAAGGATCTTGCCAAG
GATGCAGACTGGGAGGACTACCTTGGTGAATTCGCCAGTACTCCTCGCCTTGTGCAGCCGCGCGAGTATGAAGCCGCGGA
AGACATCTCCCCCCTCGAAGCCCGCTATTCCGCCAAACCCACCCTTGAAGGGCATCTGCTCTGGCAACTCCGCCTCTCTT
CCCTTACCGAAGCCCAGAAAGATATAGGCGAAATCATTATCGGCAATCTTTCATCTGCGGGCTACCTGCAGGCCAGTATT
GAAGAGGTTGCCCAGATGGCCCGGGCGGAACCCGCCGAGGCGCTGGCCGTGCTCAAACGTGTACAGATGTTTGATCCCAT
AGGCGTAGCTGCCCGCGATGCCCGCGAATGCCTGCTTGTGCAGATACGAAGCCTCAAGTATGACCGCGACCCCATTTTGC
TCGAGCTTGTCAACGCGCACCTTGAAGACCTTGAAGCCAAACGCTACAAGCCCCTGCTGCGCAAGTTCAAGCTGGACATG
GATGAACTGAAGGAATATCTCGACATCATCCAGAGCCTTGACCCCCTGCCCGGAGCGAGCTTCGGCGGCGGTGAACCGGC
CTACGTAAGTCCAGACGTGTTCGTGTATAAAATGGGCGATGAATTTATCATCCTGCTGAACGAAGACGGCCTGCCGCAGC
TCCAGCTTTCCAACCTGACACAGATGAACATGGGCACCGCTTCGGACAAGGAAAAAGAATACTGTACGGAAAAAATGCGC
TCGGCCACATGGCTGATCAAGAGCCTGTACCAGCGTCAGCGCACACTGTACAAGGTGATGGAAAGCATCGTGCGGCATCA
GCAGCCTTTTTTTGAAGAAGGCGTCACACGGCTGGCCCCCCTTATTCTCAAGGATATTGCCGACGACATCAGCATGCATG
AATCTACCGTGAGCCGCATTACCACCAATAAATACGTGGCCACGCCGCACGGTATTTTTGAACTCAAATTTTTCTTCAAC
AGCGGGCTTGAGCTGGACGACGGCAGCCAGGTCGGCTCCGAAAGCGTCAAGGCGCTCATTAAAAAATTCATTGCCGAAGA
AGACGCCAAATCGCCGCTCAGTGACGAGCGCATAGGCGAAATGCTCAAGGAACGCCTCAAGGTCAATATTGCCCGGCGAA
CTGTGGCTAAATACCGTACCGCCCTTGACATCCCCTCATCCTCCAGGCGCAAGGAGCATTTCTGA

Upstream 100 bases:

>100_bases
ATGGTGCAACCTCTGGCTATTGCGTAATTTTTGCCACTATGGCACACTACTTGTAACCACAAATATAAGGCTTCTGGATT
TTTCAAGGCGGAAAACGCAC

Downstream 100 bases:

>100_bases
TCTTTCCGCCGCGTCCCGGAACCCCGGTCCCGGCCGCCCCAACGGCGAATGACGCGCAAGATTGCGAAAAAGGGCCGTAA
ACCGCCACTGCTTCGCTGCC

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRLELLETVQQELLENPFLEESHGDDAAQEHVEDRRESGAEEAYDKDLAK
DADWEDYLGEFASTPRLVQPREYEAAEDISPLEARYSAKPTLEGHLLWQLRLSSLTEAQKDIGEIIIGNLSSAGYLQASI
EEVAQMARAEPAEALAVLKRVQMFDPIGVAARDARECLLVQIRSLKYDRDPILLELVNAHLEDLEAKRYKPLLRKFKLDM
DELKEYLDIIQSLDPLPGASFGGGEPAYVSPDVFVYKMGDEFIILLNEDGLPQLQLSNLTQMNMGTASDKEKEYCTEKMR
SATWLIKSLYQRQRTLYKVMESIVRHQQPFFEEGVTRLAPLILKDIADDISMHESTVSRITTNKYVATPHGIFELKFFFN
SGLELDDGSQVGSESVKALIKKFIAEEDAKSPLSDERIGEMLKERLKVNIARRTVAKYRTALDIPSSSRRKEHF

Sequences:

>Translated_474_residues
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRLELLETVQQELLENPFLEESHGDDAAQEHVEDRRESGAEEAYDKDLAK
DADWEDYLGEFASTPRLVQPREYEAAEDISPLEARYSAKPTLEGHLLWQLRLSSLTEAQKDIGEIIIGNLSSAGYLQASI
EEVAQMARAEPAEALAVLKRVQMFDPIGVAARDARECLLVQIRSLKYDRDPILLELVNAHLEDLEAKRYKPLLRKFKLDM
DELKEYLDIIQSLDPLPGASFGGGEPAYVSPDVFVYKMGDEFIILLNEDGLPQLQLSNLTQMNMGTASDKEKEYCTEKMR
SATWLIKSLYQRQRTLYKVMESIVRHQQPFFEEGVTRLAPLILKDIADDISMHESTVSRITTNKYVATPHGIFELKFFFN
SGLELDDGSQVGSESVKALIKKFIAEEDAKSPLSDERIGEMLKERLKVNIARRTVAKYRTALDIPSSSRRKEHF
>Mature_473_residues
ALELRQQLKLAQQLVMTPQLQQAIKLLQLSRLELLETVQQELLENPFLEESHGDDAAQEHVEDRRESGAEEAYDKDLAKD
ADWEDYLGEFASTPRLVQPREYEAAEDISPLEARYSAKPTLEGHLLWQLRLSSLTEAQKDIGEIIIGNLSSAGYLQASIE
EVAQMARAEPAEALAVLKRVQMFDPIGVAARDARECLLVQIRSLKYDRDPILLELVNAHLEDLEAKRYKPLLRKFKLDMD
ELKEYLDIIQSLDPLPGASFGGGEPAYVSPDVFVYKMGDEFIILLNEDGLPQLQLSNLTQMNMGTASDKEKEYCTEKMRS
ATWLIKSLYQRQRTLYKVMESIVRHQQPFFEEGVTRLAPLILKDIADDISMHESTVSRITTNKYVATPHGIFELKFFFNS
GLELDDGSQVGSESVKALIKKFIAEEDAKSPLSDERIGEMLKERLKVNIARRTVAKYRTALDIPSSSRRKEHF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=485, Percent_Identity=40, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53920; Mature: 53789

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRLELLETVQQELLENPFLEESHGDDAAQE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
HVEDRRESGAEEAYDKDLAKDADWEDYLGEFASTPRLVQPREYEAAEDISPLEARYSAKP
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHCCCC
TLEGHLLWQLRLSSLTEAQKDIGEIIIGNLSSAGYLQASIEEVAQMARAEPAEALAVLKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VQMFDPIGVAARDARECLLVQIRSLKYDRDPILLELVNAHLEDLEAKRYKPLLRKFKLDM
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELKEYLDIIQSLDPLPGASFGGGEPAYVSPDVFVYKMGDEFIILLNEDGLPQLQLSNLT
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCEEEEEECCCEEEEECCCCCCCHHHHCCH
QMNMGTASDKEKEYCTEKMRSATWLIKSLYQRQRTLYKVMESIVRHQQPFFEEGVTRLAP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILKDIADDISMHESTVSRITTNKYVATPHGIFELKFFFNSGLELDDGSQVGSESVKALI
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHH
KKFIAEEDAKSPLSDERIGEMLKERLKVNIARRTVAKYRTALDIPSSSRRKEHF
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ALELRQQLKLAQQLVMTPQLQQAIKLLQLSRLELLETVQQELLENPFLEESHGDDAAQE
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
HVEDRRESGAEEAYDKDLAKDADWEDYLGEFASTPRLVQPREYEAAEDISPLEARYSAKP
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHCCCC
TLEGHLLWQLRLSSLTEAQKDIGEIIIGNLSSAGYLQASIEEVAQMARAEPAEALAVLKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VQMFDPIGVAARDARECLLVQIRSLKYDRDPILLELVNAHLEDLEAKRYKPLLRKFKLDM
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELKEYLDIIQSLDPLPGASFGGGEPAYVSPDVFVYKMGDEFIILLNEDGLPQLQLSNLT
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCEEEEEECCCEEEEECCCCCCCHHHHCCH
QMNMGTASDKEKEYCTEKMRSATWLIKSLYQRQRTLYKVMESIVRHQQPFFEEGVTRLAP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILKDIADDISMHESTVSRITTNKYVATPHGIFELKFFFNSGLELDDGSQVGSESVKALI
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHH
KKFIAEEDAKSPLSDERIGEMLKERLKVNIARRTVAKYRTALDIPSSSRRKEHF
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]