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The map label for this gene is cysG2 [H]

Identifier: 220904234

GI number: 220904234

Start: 1120525

End: 1121229

Strand: Reverse

Name: cysG2 [H]

Synonym: Ddes_0961

Alternate gene names: 220904234

Gene position: 1121229-1120525 (Counterclockwise)

Preceding gene: 220904237

Following gene: 220904233

Centisome position: 39.02

GC content: 61.7

Gene sequence:

>705_bases
ATGGAAACACCCGCAGCCAGGCCGGCTTTTTATCCACTCTTTCTTTCCCTTGAAGGCATGCGCTGCCTTGTGGCGGGCCT
TGGTCAGGTAGGTCGCCGCAAGCTGGAAGGACTGCTGGCGTGCGGCCCGGCTTTTGTGCTTGTACTGGATATTGACGAAC
CTTCGCCCGGCGATACAGCCCTGCAAGACTTGCTGCGCCAGGGCAATGTGCGCTTTGAACGCCGCGCCTGCGTGGAAAAC
GACATGCAGGGCATGGCCCTTGTGTTCGCCACAACAGGCAACGATGCGGAAAACAGGCGTATCGCCGCCATCTGCCGCCG
TAACGGTGTATTGTGCAACTGCGCAAGCGCCCCGGAAGAAGGCAGTTTTCAGGTTCCTGCGGTGGCCCGCAAAACGCCCC
TGGCGGCGGCGCTTTCAACAGGTGGCGCAAGCCCGGCCCTGGCCCGGCGCTGGAAAGGCGAACTGGTCTGCTGGCTGGCT
CCGCGGGCGCGCATGGCCTGCCTGATGGGCCGCCTGCGGCCGCTTGTCCTTGCTTTGGGCGGCGAGACAGGGCAGAATAC
TGAGCTGTTCCGGAAACTGGCGGCCTCTCCCCTGCAGCAATGGCTGGAGGAAAAGGAAATGGAAAACTGCCGCCAGTGGC
TGTTGGCGGAATTGCCAACGGAACTGCACGCTCATATAGCGGAGTTACTTTATGATCTCCCCTGA

Upstream 100 bases:

>100_bases
AGCAAGGGCGGGGCCTTTGCAAAACGTGAAGGCTGCTGTATCATAGAATATATCGAAAAGACATGCGGTTTTTCGCATCA
TGCTACACAACGGATCCCAC

Downstream 100 bases:

>100_bases
ATTTTCCACCGGCGTCACGCTGCTGCTCTACGGCCTTGCCAGCGTGTCCGGCATAGTCGGCATGGTAGCGCGCAGCCCTT
TCTGGCGAAAACTTGGCTGC

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
METPAARPAFYPLFLSLEGMRCLVAGLGQVGRRKLEGLLACGPAFVLVLDIDEPSPGDTALQDLLRQGNVRFERRACVEN
DMQGMALVFATTGNDAENRRIAAICRRNGVLCNCASAPEEGSFQVPAVARKTPLAAALSTGGASPALARRWKGELVCWLA
PRARMACLMGRLRPLVLALGGETGQNTELFRKLAASPLQQWLEEKEMENCRQWLLAELPTELHAHIAELLYDLP

Sequences:

>Translated_234_residues
METPAARPAFYPLFLSLEGMRCLVAGLGQVGRRKLEGLLACGPAFVLVLDIDEPSPGDTALQDLLRQGNVRFERRACVEN
DMQGMALVFATTGNDAENRRIAAICRRNGVLCNCASAPEEGSFQVPAVARKTPLAAALSTGGASPALARRWKGELVCWLA
PRARMACLMGRLRPLVLALGGETGQNTELFRKLAASPLQQWLEEKEMENCRQWLLAELPTELHAHIAELLYDLP
>Mature_234_residues
METPAARPAFYPLFLSLEGMRCLVAGLGQVGRRKLEGLLACGPAFVLVLDIDEPSPGDTALQDLLRQGNVRFERRACVEN
DMQGMALVFATTGNDAENRRIAAICRRNGVLCNCASAPEEGSFQVPAVARKTPLAAALSTGGASPALARRWKGELVCWLA
PRARMACLMGRLRPLVLALGGETGQNTELFRKLAASPLQQWLEEKEMENCRQWLLAELPTELHAHIAELLYDLP

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=183, Percent_Identity=30.0546448087432, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 25537; Mature: 25537

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPAARPAFYPLFLSLEGMRCLVAGLGQVGRRKLEGLLACGPAFVLVLDIDEPSPGDTA
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
LQDLLRQGNVRFERRACVENDMQGMALVFATTGNDAENRRIAAICRRNGVLCNCASAPEE
HHHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCC
GSFQVPAVARKTPLAAALSTGGASPALARRWKGELVCWLAPRARMACLMGRLRPLVLALG
CCEECCHHHHHCCHHHHHHCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHEEEEC
GETGQNTELFRKLAASPLQQWLEEKEMENCRQWLLAELPTELHAHIAELLYDLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
METPAARPAFYPLFLSLEGMRCLVAGLGQVGRRKLEGLLACGPAFVLVLDIDEPSPGDTA
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
LQDLLRQGNVRFERRACVENDMQGMALVFATTGNDAENRRIAAICRRNGVLCNCASAPEE
HHHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCC
GSFQVPAVARKTPLAAALSTGGASPALARRWKGELVCWLAPRARMACLMGRLRPLVLALG
CCEECCHHHHHCCHHHHHHCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHEEEEC
GETGQNTELFRKLAASPLQQWLEEKEMENCRQWLLAELPTELHAHIAELLYDLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA