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The map label for this gene is zraS [H]

Identifier: 220904125

GI number: 220904125

Start: 996825

End: 998411

Strand: Direct

Name: zraS [H]

Synonym: Ddes_0852

Alternate gene names: 220904125

Gene position: 996825-998411 (Clockwise)

Preceding gene: 220904124

Following gene: 220904126

Centisome position: 34.69

GC content: 58.98

Gene sequence:

>1587_bases
ATGTGGACGTTCTTTAGAAAAAAACAGGCTCCGCCTCCTGATGCCATTGCTACGGCAAGCGGGCAGGACGGCGCGGCGCA
GGCCGGAAGCGGCCGCGGCATGCCGTCGGGCAGCAGCCAGGATTGCGGCCAGACAGGCTCTACGGCCGGCTCCCGGGGTG
ATGCCGGCGAGGGGTTGCCCCTGAGTTACGCCCGGCCCCTGTCCTGGCTTTCGCTGGTGATTATTCTGCTCACCAGTCTG
GTGCTTTCGTTCTTCATTTCCAATTCAGCCCGCGAAACCCTGCTGACCCGGCAGGAGGGTTTCGCCCGCCTGCTGGTGGA
AAACCTCAACAGCCAGATTTTCCGGCGCTTTGCCTTGCCCACGCTTTTCGGCTACGGGCGCATTTCCTTGCGGCAGCCCG
CCCAGTACGACCGGCTGGATCAGGTGGTGCAGTCCGTGATTCACGGTCTGCCTGTGGAACGTCTGCGCATCTATGATTTT
TCACGCATTGTGGCATATTCCACCCGCAAGGAAGAAATCGGCCGTGCCGGGCTTTCTCCGGCCAATCTGGACGAGGTGTT
GCACGGCGCGGCCCCCAAGTCTGAAATAATTTCGACCATACCGGCATGGCAGGCCCCTTTCAGGCTCCCGCTTGAAGAAG
GAACCTTTGTTCTCCGTGTGCTGTACCCCTTGCGGGGTGAATCGCTCAAGCCCGGTGAGGATGCCCCCATCATGGGCGCG
CTGGAGCTTACACAGGACATTACGGGCGACTATGAGCAGGTGGTGACGTTTCAGGGCATTATTGTAATCATGTGCCTCAT
GTCTTCGGTGGTGCTTTTCGGCCTTTTGATCATGCTTATCCACAGGTCGGAGCGTGTACTGGCCGAGCGGATGAATAAAA
ACCGCCAGCTCGAGAAGCAGCTTCACAGCAACGAACGTTTTGTGAGCATGGGCCGCGTGATCGCCAGTATTGCCCACGAG
ATCAGAAATCCTCTCGGCATCATCCGCTCCAGCGCCGAGCTGCTGCAACGCCGCAGCGATGCCGATGCAGGAACCCGGCG
TATACTGGGAGCCATTTTTGACGAATCTGTGCGCCTTTCACAAACCGTAAACGACTTTCTTGACTATGCCCGCCCCCGGC
AACCCAAGCAGGACCTGGTTGATATAAGCCTTGTGCTGGACCAGGTGCTGGGCTTTCTGGAGGGTGATATGGGCCGCCGC
AACGTGGCGCTGGAACGCAATACTGAAGAGGGCCTTTTCGTTCATGGCGACAAGGATTTGCTGTACCGCGCTTTTTACAA
TATTCTGGTGAACGGACAACAGGCAATGGACGGCCCGGGCGTACTGCACATCAGTGGAGTTCATGACGGCGAGGGGCATG
TGCGGCTGGAGTTTCTCGACTCCGGTCCCGGTTTTGACGCCTCCGTTCTGGCCAACCTGCTGGACCCCTTCTTTACGACC
AAAGACGGGGGAACTGGCCTTGGCCTGCCCATTGTGCAGTCCATTATCACAAGCCACGGCGGCGTGATCCAACTGGAAAA
CGGCCCGGAAGGCGGCGCTCTTGTACGTGTGCTGCTGCCCGAAGCCCATGCCGGGGTACAGGAATAG

Upstream 100 bases:

>100_bases
TCTTGCCAACGCCCGCCTGGGTGCGCCGGGCTATGAGCGTGAACAGACGGATCTGGACAGTTTTATGGCCGTACCGTTGC
AGGAGCAGGTGTTTTAGGGC

Downstream 100 bases:

>100_bases
TCTCATGAGTGAAAAAGCGCATATTCTGATTATTGACGACGAAAAAAACTACCTTCTTGTTCTGCAGACCCTGCTGGAAG
ATGAAGGGTACACCGTTACC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MWTFFRKKQAPPPDAIATASGQDGAAQAGSGRGMPSGSSQDCGQTGSTAGSRGDAGEGLPLSYARPLSWLSLVIILLTSL
VLSFFISNSARETLLTRQEGFARLLVENLNSQIFRRFALPTLFGYGRISLRQPAQYDRLDQVVQSVIHGLPVERLRIYDF
SRIVAYSTRKEEIGRAGLSPANLDEVLHGAAPKSEIISTIPAWQAPFRLPLEEGTFVLRVLYPLRGESLKPGEDAPIMGA
LELTQDITGDYEQVVTFQGIIVIMCLMSSVVLFGLLIMLIHRSERVLAERMNKNRQLEKQLHSNERFVSMGRVIASIAHE
IRNPLGIIRSSAELLQRRSDADAGTRRILGAIFDESVRLSQTVNDFLDYARPRQPKQDLVDISLVLDQVLGFLEGDMGRR
NVALERNTEEGLFVHGDKDLLYRAFYNILVNGQQAMDGPGVLHISGVHDGEGHVRLEFLDSGPGFDASVLANLLDPFFTT
KDGGTGLGLPIVQSIITSHGGVIQLENGPEGGALVRVLLPEAHAGVQE

Sequences:

>Translated_528_residues
MWTFFRKKQAPPPDAIATASGQDGAAQAGSGRGMPSGSSQDCGQTGSTAGSRGDAGEGLPLSYARPLSWLSLVIILLTSL
VLSFFISNSARETLLTRQEGFARLLVENLNSQIFRRFALPTLFGYGRISLRQPAQYDRLDQVVQSVIHGLPVERLRIYDF
SRIVAYSTRKEEIGRAGLSPANLDEVLHGAAPKSEIISTIPAWQAPFRLPLEEGTFVLRVLYPLRGESLKPGEDAPIMGA
LELTQDITGDYEQVVTFQGIIVIMCLMSSVVLFGLLIMLIHRSERVLAERMNKNRQLEKQLHSNERFVSMGRVIASIAHE
IRNPLGIIRSSAELLQRRSDADAGTRRILGAIFDESVRLSQTVNDFLDYARPRQPKQDLVDISLVLDQVLGFLEGDMGRR
NVALERNTEEGLFVHGDKDLLYRAFYNILVNGQQAMDGPGVLHISGVHDGEGHVRLEFLDSGPGFDASVLANLLDPFFTT
KDGGTGLGLPIVQSIITSHGGVIQLENGPEGGALVRVLLPEAHAGVQE
>Mature_528_residues
MWTFFRKKQAPPPDAIATASGQDGAAQAGSGRGMPSGSSQDCGQTGSTAGSRGDAGEGLPLSYARPLSWLSLVIILLTSL
VLSFFISNSARETLLTRQEGFARLLVENLNSQIFRRFALPTLFGYGRISLRQPAQYDRLDQVVQSVIHGLPVERLRIYDF
SRIVAYSTRKEEIGRAGLSPANLDEVLHGAAPKSEIISTIPAWQAPFRLPLEEGTFVLRVLYPLRGESLKPGEDAPIMGA
LELTQDITGDYEQVVTFQGIIVIMCLMSSVVLFGLLIMLIHRSERVLAERMNKNRQLEKQLHSNERFVSMGRVIASIAHE
IRNPLGIIRSSAELLQRRSDADAGTRRILGAIFDESVRLSQTVNDFLDYARPRQPKQDLVDISLVLDQVLGFLEGDMGRR
NVALERNTEEGLFVHGDKDLLYRAFYNILVNGQQAMDGPGVLHISGVHDGEGHVRLEFLDSGPGFDASVLANLLDPFFTT
KDGGTGLGLPIVQSIITSHGGVIQLENGPEGGALVRVLLPEAHAGVQE

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=262, Percent_Identity=29.3893129770992, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=29.1139240506329, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788393, Length=251, Percent_Identity=29.4820717131474, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=28.8617886178862, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=29.3859649122807, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=27.5720164609054, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=28.310502283105, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=29.4642857142857, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57757; Mature: 57757

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTFFRKKQAPPPDAIATASGQDGAAQAGSGRGMPSGSSQDCGQTGSTAGSRGDAGEGLP
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LSYARPLSWLSLVIILLTSLVLSFFISNSARETLLTRQEGFARLLVENLNSQIFRRFALP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFGYGRISLRQPAQYDRLDQVVQSVIHGLPVERLRIYDFSRIVAYSTRKEEIGRAGLSP
HHHCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
ANLDEVLHGAAPKSEIISTIPAWQAPFRLPLEEGTFVLRVLYPLRGESLKPGEDAPIMGA
CCHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCHH
LELTQDITGDYEQVVTFQGIIVIMCLMSSVVLFGLLIMLIHRSERVLAERMNKNRQLEKQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LHSNERFVSMGRVIASIAHEIRNPLGIIRSSAELLQRRSDADAGTRRILGAIFDESVRLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHH
QTVNDFLDYARPRQPKQDLVDISLVLDQVLGFLEGDMGRRNVALERNTEEGLFVHGDKDL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCHHH
LYRAFYNILVNGQQAMDGPGVLHISGVHDGEGHVRLEFLDSGPGFDASVLANLLDPFFTT
HHHHHHHHHHCCCHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KDGGTGLGLPIVQSIITSHGGVIQLENGPEGGALVRVLLPEAHAGVQE
CCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHCCCCCC
>Mature Secondary Structure
MWTFFRKKQAPPPDAIATASGQDGAAQAGSGRGMPSGSSQDCGQTGSTAGSRGDAGEGLP
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LSYARPLSWLSLVIILLTSLVLSFFISNSARETLLTRQEGFARLLVENLNSQIFRRFALP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFGYGRISLRQPAQYDRLDQVVQSVIHGLPVERLRIYDFSRIVAYSTRKEEIGRAGLSP
HHHCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
ANLDEVLHGAAPKSEIISTIPAWQAPFRLPLEEGTFVLRVLYPLRGESLKPGEDAPIMGA
CCHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCHH
LELTQDITGDYEQVVTFQGIIVIMCLMSSVVLFGLLIMLIHRSERVLAERMNKNRQLEKQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LHSNERFVSMGRVIASIAHEIRNPLGIIRSSAELLQRRSDADAGTRRILGAIFDESVRLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHH
QTVNDFLDYARPRQPKQDLVDISLVLDQVLGFLEGDMGRRNVALERNTEEGLFVHGDKDL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCHHH
LYRAFYNILVNGQQAMDGPGVLHISGVHDGEGHVRLEFLDSGPGFDASVLANLLDPFFTT
HHHHHHHHHHCCCHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KDGGTGLGLPIVQSIITSHGGVIQLENGPEGGALVRVLLPEAHAGVQE
CCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]