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The map label for this gene is hyaA [H]

Identifier: 220904111

GI number: 220904111

Start: 983574

End: 984677

Strand: Reverse

Name: hyaA [H]

Synonym: Ddes_0837

Alternate gene names: 220904111

Gene position: 984677-983574 (Counterclockwise)

Preceding gene: 220904112

Following gene: 220904110

Centisome position: 34.27

GC content: 61.14

Gene sequence:

>1104_bases
ATGGCACGTCTTGAAACAACCGCCGAGGTTCTGCGCAGCAAAGGCGTCAGCCGCCGGGACTTCATGAAGTTCTGCGCGCT
CACAGCCGTTGCAATGGGGCTTGGCCCCGGCGCGGACCTGGCTATTGCCCAGGCCCTTTCCACCAAACCCCGTCTTCCGG
TACTCTGGATCAATGGTCTTTCCTGCTCCTGCTGTACCGAATCCTTCCTGCGCACGGCCCACCCCCTGGCGGCCGACATT
ATTCTTTCAATGATTGCCCTGGACTATCAGGACACCATCATGGCAGCTGCCGGCGAACAGGCCAATGCCTGCTACGAGCA
GGCCATTGAAAGGTACAAGGGCCAGTACATCCTGGCTGTGGAAGGCAACGTGCCCCTGAATGAACAGGGCATGTACTGCA
TTGACGGCGGCAAACCCTTTTATGAAAAGCTCAAGCGCGGCGTGGAGCATGCCAAGGCCGTGGTCGCCTGGGGCACCTGT
GCCTCCTGGGGCTGCGTGCAGGCGGCTTCGCCCAATCCCACTGCGGCCACCCCCCTGCACAAGCTTTTCCCCAACAAACC
GCAGATCAAGGTTCCCGGCTGTCCGGCCATTCCCGAGGTCATGAGTTCCATCCTGACCTACATCATCACCTATGACCGCC
TGCCGAGCCTTGATGCACAAGGGCGGCCCGAAATGTTCTACGGCGTGCGCGTACACGACCAGTGCTACCGCCGTGCCCAC
TTTGACGCGGGCCAGTTTGTAGAATCGTGGGACGACGAGGGCGCGCGCTCCGGCCTGTGCCTGTACAAGATGGGCTGCAA
AGGCCCCACCACCTACAATGCCTGCCCCTCCACCCGCTGGAACAACGGCGTTTCCTTTCCCATCCAGTCCGGGCATGGCT
GCATTGGCTGCTCGGAACAGAACTTCTGGGATCAGCAGTCCTTTTACGACCGCATCACCACCATCCCGCATATGGGAACC
AACTCCACGGCTGAAAGCGTGGGGGTGGCCGCAGTGGCGGGCGTGGCCGCCGGTGTGGCTATTCACGGCGCGGCCAGCAT
GGTCCGTCATGCCTGCGACAAGAAGAAAGCCCCGGAAGCTAACGAAGACACCAGCAAGAGCTAA

Upstream 100 bases:

>100_bases
ATGATAAAGCGGGCTTGAGTATAGCCCCTTCTCTGCCCGGTGCAGCTTTTGCACCCCCTCCTCACAGCTCACTTCCAACC
TAGCAAGCGTTGAGGTAACT

Downstream 100 bases:

>100_bases
GGAACGTACCCATGTCCTATGAATACAAGACACAAGGATATACTGTTGTTGACGCGGGCCGCCGCCTCGTTGTGGACCCC
GTGACCCGCATTGAAGGTCA

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MARLETTAEVLRSKGVSRRDFMKFCALTAVAMGLGPGADLAIAQALSTKPRLPVLWINGLSCSCCTESFLRTAHPLAADI
ILSMIALDYQDTIMAAAGEQANACYEQAIERYKGQYILAVEGNVPLNEQGMYCIDGGKPFYEKLKRGVEHAKAVVAWGTC
ASWGCVQAASPNPTAATPLHKLFPNKPQIKVPGCPAIPEVMSSILTYIITYDRLPSLDAQGRPEMFYGVRVHDQCYRRAH
FDAGQFVESWDDEGARSGLCLYKMGCKGPTTYNACPSTRWNNGVSFPIQSGHGCIGCSEQNFWDQQSFYDRITTIPHMGT
NSTAESVGVAAVAGVAAGVAIHGAASMVRHACDKKKAPEANEDTSKS

Sequences:

>Translated_367_residues
MARLETTAEVLRSKGVSRRDFMKFCALTAVAMGLGPGADLAIAQALSTKPRLPVLWINGLSCSCCTESFLRTAHPLAADI
ILSMIALDYQDTIMAAAGEQANACYEQAIERYKGQYILAVEGNVPLNEQGMYCIDGGKPFYEKLKRGVEHAKAVVAWGTC
ASWGCVQAASPNPTAATPLHKLFPNKPQIKVPGCPAIPEVMSSILTYIITYDRLPSLDAQGRPEMFYGVRVHDQCYRRAH
FDAGQFVESWDDEGARSGLCLYKMGCKGPTTYNACPSTRWNNGVSFPIQSGHGCIGCSEQNFWDQQSFYDRITTIPHMGT
NSTAESVGVAAVAGVAAGVAIHGAASMVRHACDKKKAPEANEDTSKS
>Mature_366_residues
ARLETTAEVLRSKGVSRRDFMKFCALTAVAMGLGPGADLAIAQALSTKPRLPVLWINGLSCSCCTESFLRTAHPLAADII
LSMIALDYQDTIMAAAGEQANACYEQAIERYKGQYILAVEGNVPLNEQGMYCIDGGKPFYEKLKRGVEHAKAVVAWGTCA
SWGCVQAASPNPTAATPLHKLFPNKPQIKVPGCPAIPEVMSSILTYIITYDRLPSLDAQGRPEMFYGVRVHDQCYRRAHF
DAGQFVESWDDEGARSGLCLYKMGCKGPTTYNACPSTRWNNGVSFPIQSGHGCIGCSEQNFWDQQSFYDRITTIPHMGTN
STAESVGVAAVAGVAAGVAIHGAASMVRHACDKKKAPEANEDTSKS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=333, Percent_Identity=65.4654654654655, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1789371, Length=330, Percent_Identity=39.0909090909091, Blast_Score=267, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39618; Mature: 39486

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLETTAEVLRSKGVSRRDFMKFCALTAVAMGLGPGADLAIAQALSTKPRLPVLWINGL
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCC
SCSCCTESFLRTAHPLAADIILSMIALDYQDTIMAAAGEQANACYEQAIERYKGQYILAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
EGNVPLNEQGMYCIDGGKPFYEKLKRGVEHAKAVVAWGTCASWGCVQAASPNPTAATPLH
ECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCEECCCCCCCCCCCHH
KLFPNKPQIKVPGCPAIPEVMSSILTYIITYDRLPSLDAQGRPEMFYGVRVHDQCYRRAH
HHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHC
FDAGQFVESWDDEGARSGLCLYKMGCKGPTTYNACPSTRWNNGVSFPIQSGHGCIGCSEQ
CCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCC
NFWDQQSFYDRITTIPHMGTNSTAESVGVAAVAGVAAGVAIHGAASMVRHACDKKKAPEA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEDTSKS
CCCCCCC
>Mature Secondary Structure 
ARLETTAEVLRSKGVSRRDFMKFCALTAVAMGLGPGADLAIAQALSTKPRLPVLWINGL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCC
SCSCCTESFLRTAHPLAADIILSMIALDYQDTIMAAAGEQANACYEQAIERYKGQYILAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
EGNVPLNEQGMYCIDGGKPFYEKLKRGVEHAKAVVAWGTCASWGCVQAASPNPTAATPLH
ECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCEECCCCCCCCCCCHH
KLFPNKPQIKVPGCPAIPEVMSSILTYIITYDRLPSLDAQGRPEMFYGVRVHDQCYRRAH
HHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHC
FDAGQFVESWDDEGARSGLCLYKMGCKGPTTYNACPSTRWNNGVSFPIQSGHGCIGCSEQ
CCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCC
NFWDQQSFYDRITTIPHMGTNSTAESVGVAAVAGVAAGVAIHGAASMVRHACDKKKAPEA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEDTSKS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA