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The map label for this gene is hyaB [H]

Identifier: 220904110

GI number: 220904110

Start: 981748

End: 983562

Strand: Reverse

Name: hyaB [H]

Synonym: Ddes_0836

Alternate gene names: 220904110

Gene position: 983562-981748 (Counterclockwise)

Preceding gene: 220904111

Following gene: 220904109

Centisome position: 34.23

GC content: 59.89

Gene sequence:

>1815_bases
ATGTCCTATGAATACAAGACACAAGGATATACTGTTGTTGACGCGGGCCGCCGCCTCGTTGTGGACCCCGTGACCCGCAT
TGAAGGTCATCTGCGCTGCGAAGTGAATATCAACGACGACAACGTGATCACCAACGCCGTATCCTGCGGCACCATTTTTC
GCGGCATAGAAATCATCCTCAAAGGACGCGACCCCCGCGATGCCTGGGCCTTTACCGAGCGCATCTGCGGTGTATGCACC
GGTACGCATGCCCTGGCCTCGGTGCATGCCGTGGAAAACGCCCTGGGCATTGATATTCCCGACAATGCCAACATCATCCG
TAACCTCATGCAGCTCTGCCTTATGTACCACGACCACCTCGTGCACCTGTACCACCTCACGGGGCTGGACTGGGTGGACG
TGGTTTCGGCCTCCAAGGCCGACCCCAAGGCAACGTCCGAGCTGGCACAAAAACTGTCGCCCTGGCCCAACTCTTCGCCG
GGCTACTTCAAGTCGGTCAAAGACAAGCTGCTCAAGCTCATCAATTCCAACCAGCTTGGCATATTCACCAACGGCTACTG
GGGACACCCCGCCTACAAGCTCTCGCCCGAAGCCAACCTTATGGTGGTCGCCCACTATATCGAGGCTCTGGATTTCCAGA
AAGACGTGGTGCACATCCACACCATCTTCGGCGGCAAAAACCCCCATCCCAACTGGCTGGTGGGGGGCGTGCCCTGCTCG
CTCAACATTGACGGCGTGGGCGCGGCCGATGTCATCAATCAGGAACGCCTGGACTTCGTGCTTCAGGTTATCGAGCGCTG
CCGTACCTTTGCCCAGCAGGTGGTTATTCCGGACACGCTCGCTCTGGCCGGCTTTTACGGCGACTGGCTCAACATCGGCG
GCGGCCTGTCCAAACTCTCGGTGCTGGCCTATGGCGGCATTCCCGACATTGCCAACGACTATACCGAAAACAGCCTGCTC
ATGCCTCCCGGGGCCATCATCAACGGCAACTTCAACGAAGTGCTGCCCGTGAGTCTGACCAATCCCGAAGAAATCCAGGA
AACCGTCAACCATTCCTGGTACAAGCCCTACCCCGCTGGAAAAACCGGCCTGCACCCCTGGGAGGGCATCACCGAACCGC
ACTACAATCCCGGCCCCAATATCAAGGGTACCCCCACAGACCTCAAGCAGGTGGACGAACGGGACCGTTACTCCTGGCTC
AAGACCCCCCTGTGGCGCGGGCACCAGATGGAAGTCGGCCCCCTGGCCCGCATGCTCATCGGCTACGCCCGTAAAAATGA
CGACATCAAGGGCCTTGTGGACGACTTCCTGGGGCGCGCAGGCGGCGTGCCTGTTACGGTGCTGCAATCCACACTGGGGC
GCATAGCCGCCCGCTCTCTGGAACTGGCATGGGCTGCTGAAAAAATGCGCTATTTTTACGATCGCCTTATCACCAACCTC
AAGAACGGAGTCCGCGCCACAGCCAACACCACCAAGTGGAAGCCCGAATCCTGGCCCACCGGCGAGTTGCGGGGCGTGGG
CTTTACCGAAGCGCCGCGCGGCGCTCTCGGTCACTGGGTTAAAATCAGGGACAGAAAAATCGAAAGCTACCAGTGCGTGG
TCCCCACCACCTGGAACGGCGCACCGCGCAGCCCCGACGGCCAACTGAGCGCTTACGAGGCCTCGCTGATGAATACCCGC
ATGGCCATACCCGAACAGCCCCTGGAAATCCTGCGCACCCTGCACAGCTTTGACCCCTGCCTTGCCTGCTCGACCCACAT
TATTGGCCCCGACGGCAAGGACCTGCTCACTGTGCGCATGGACCGAGGCATGTAG

Upstream 100 bases:

>100_bases
TGGCTATTCACGGCGCGGCCAGCATGGTCCGTCATGCCTGCGACAAGAAGAAAGCCCCGGAAGCTAACGAAGACACCAGC
AAGAGCTAAGGAACGTACCC

Downstream 100 bases:

>100_bases
CTCCGGCCCCGCTGACGCCCGGCGCGTAAGCGCCCCAAGCGTAGGAGAACGATATGAGCGATACAAGACAAAGCACGCCC
GAAGTACCCATGGGCAAAAG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MSYEYKTQGYTVVDAGRRLVVDPVTRIEGHLRCEVNINDDNVITNAVSCGTIFRGIEIILKGRDPRDAWAFTERICGVCT
GTHALASVHAVENALGIDIPDNANIIRNLMQLCLMYHDHLVHLYHLTGLDWVDVVSASKADPKATSELAQKLSPWPNSSP
GYFKSVKDKLLKLINSNQLGIFTNGYWGHPAYKLSPEANLMVVAHYIEALDFQKDVVHIHTIFGGKNPHPNWLVGGVPCS
LNIDGVGAADVINQERLDFVLQVIERCRTFAQQVVIPDTLALAGFYGDWLNIGGGLSKLSVLAYGGIPDIANDYTENSLL
MPPGAIINGNFNEVLPVSLTNPEEIQETVNHSWYKPYPAGKTGLHPWEGITEPHYNPGPNIKGTPTDLKQVDERDRYSWL
KTPLWRGHQMEVGPLARMLIGYARKNDDIKGLVDDFLGRAGGVPVTVLQSTLGRIAARSLELAWAAEKMRYFYDRLITNL
KNGVRATANTTKWKPESWPTGELRGVGFTEAPRGALGHWVKIRDRKIESYQCVVPTTWNGAPRSPDGQLSAYEASLMNTR
MAIPEQPLEILRTLHSFDPCLACSTHIIGPDGKDLLTVRMDRGM

Sequences:

>Translated_604_residues
MSYEYKTQGYTVVDAGRRLVVDPVTRIEGHLRCEVNINDDNVITNAVSCGTIFRGIEIILKGRDPRDAWAFTERICGVCT
GTHALASVHAVENALGIDIPDNANIIRNLMQLCLMYHDHLVHLYHLTGLDWVDVVSASKADPKATSELAQKLSPWPNSSP
GYFKSVKDKLLKLINSNQLGIFTNGYWGHPAYKLSPEANLMVVAHYIEALDFQKDVVHIHTIFGGKNPHPNWLVGGVPCS
LNIDGVGAADVINQERLDFVLQVIERCRTFAQQVVIPDTLALAGFYGDWLNIGGGLSKLSVLAYGGIPDIANDYTENSLL
MPPGAIINGNFNEVLPVSLTNPEEIQETVNHSWYKPYPAGKTGLHPWEGITEPHYNPGPNIKGTPTDLKQVDERDRYSWL
KTPLWRGHQMEVGPLARMLIGYARKNDDIKGLVDDFLGRAGGVPVTVLQSTLGRIAARSLELAWAAEKMRYFYDRLITNL
KNGVRATANTTKWKPESWPTGELRGVGFTEAPRGALGHWVKIRDRKIESYQCVVPTTWNGAPRSPDGQLSAYEASLMNTR
MAIPEQPLEILRTLHSFDPCLACSTHIIGPDGKDLLTVRMDRGM
>Mature_603_residues
SYEYKTQGYTVVDAGRRLVVDPVTRIEGHLRCEVNINDDNVITNAVSCGTIFRGIEIILKGRDPRDAWAFTERICGVCTG
THALASVHAVENALGIDIPDNANIIRNLMQLCLMYHDHLVHLYHLTGLDWVDVVSASKADPKATSELAQKLSPWPNSSPG
YFKSVKDKLLKLINSNQLGIFTNGYWGHPAYKLSPEANLMVVAHYIEALDFQKDVVHIHTIFGGKNPHPNWLVGGVPCSL
NIDGVGAADVINQERLDFVLQVIERCRTFAQQVVIPDTLALAGFYGDWLNIGGGLSKLSVLAYGGIPDIANDYTENSLLM
PPGAIINGNFNEVLPVSLTNPEEIQETVNHSWYKPYPAGKTGLHPWEGITEPHYNPGPNIKGTPTDLKQVDERDRYSWLK
TPLWRGHQMEVGPLARMLIGYARKNDDIKGLVDDFLGRAGGVPVTVLQSTLGRIAARSLELAWAAEKMRYFYDRLITNLK
NGVRATANTTKWKPESWPTGELRGVGFTEAPRGALGHWVKIRDRKIESYQCVVPTTWNGAPRSPDGQLSAYEASLMNTRM
AIPEQPLEILRTLHSFDPCLACSTHIIGPDGKDLLTVRMDRGM

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=600, Percent_Identity=64.5, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=594, Percent_Identity=43.0976430976431, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66972; Mature: 66841

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEYKTQGYTVVDAGRRLVVDPVTRIEGHLRCEVNINDDNVITNAVSCGTIFRGIEIIL
CCCEECCCCEEEEECCCEEEECCHHHCCCEEEEEEEECCCCEEEHHHHHHHHHHCEEEEE
KGRDPRDAWAFTERICGVCTGTHALASVHAVENALGIDIPDNANIIRNLMQLCLMYHDHL
ECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VHLYHLTGLDWVDVVSASKADPKATSELAQKLSPWPNSSPGYFKSVKDKLLKLINSNQLG
EEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
IFTNGYWGHPAYKLSPEANLMVVAHYIEALDFQKDVVHIHTIFGGKNPHPNWLVGGVPCS
EEECCCCCCCCEEECCCCCEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECEEEE
LNIDGVGAADVINQERLDFVLQVIERCRTFAQQVVIPDTLALAGFYGDWLNIGGGLSKLS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHE
VLAYGGIPDIANDYTENSLLMPPGAIINGNFNEVLPVSLTNPEEIQETVNHSWYKPYPAG
EEEECCCCHHHHCCCCCCEECCCCCEECCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCC
KTGLHPWEGITEPHYNPGPNIKGTPTDLKQVDERDRYSWLKTPLWRGHQMEVGPLARMLI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GYARKNDDIKGLVDDFLGRAGGVPVTVLQSTLGRIAARSLELAWAAEKMRYFYDRLITNL
HHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNGVRATANTTKWKPESWPTGELRGVGFTEAPRGALGHWVKIRDRKIESYQCVVPTTWNG
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEECCCCCCEEEEEEECCCC
APRSPDGQLSAYEASLMNTRMAIPEQPLEILRTLHSFDPCLACSTHIIGPDGKDLLTVRM
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEEE
DRGM
CCCC
>Mature Secondary Structure 
SYEYKTQGYTVVDAGRRLVVDPVTRIEGHLRCEVNINDDNVITNAVSCGTIFRGIEIIL
CCEECCCCEEEEECCCEEEECCHHHCCCEEEEEEEECCCCEEEHHHHHHHHHHCEEEEE
KGRDPRDAWAFTERICGVCTGTHALASVHAVENALGIDIPDNANIIRNLMQLCLMYHDHL
ECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VHLYHLTGLDWVDVVSASKADPKATSELAQKLSPWPNSSPGYFKSVKDKLLKLINSNQLG
EEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
IFTNGYWGHPAYKLSPEANLMVVAHYIEALDFQKDVVHIHTIFGGKNPHPNWLVGGVPCS
EEECCCCCCCCEEECCCCCEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECEEEE
LNIDGVGAADVINQERLDFVLQVIERCRTFAQQVVIPDTLALAGFYGDWLNIGGGLSKLS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHE
VLAYGGIPDIANDYTENSLLMPPGAIINGNFNEVLPVSLTNPEEIQETVNHSWYKPYPAG
EEEECCCCHHHHCCCCCCEECCCCCEECCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCC
KTGLHPWEGITEPHYNPGPNIKGTPTDLKQVDERDRYSWLKTPLWRGHQMEVGPLARMLI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GYARKNDDIKGLVDDFLGRAGGVPVTVLQSTLGRIAARSLELAWAAEKMRYFYDRLITNL
HHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNGVRATANTTKWKPESWPTGELRGVGFTEAPRGALGHWVKIRDRKIESYQCVVPTTWNG
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEECCCCCCEEEEEEECCCC
APRSPDGQLSAYEASLMNTRMAIPEQPLEILRTLHSFDPCLACSTHIIGPDGKDLLTVRM
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEEE
DRGM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA