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The map label for this gene is gacA [H]

Identifier: 220904084

GI number: 220904084

Start: 949635

End: 950306

Strand: Direct

Name: gacA [H]

Synonym: Ddes_0810

Alternate gene names: 220904084

Gene position: 949635-950306 (Clockwise)

Preceding gene: 220904083

Following gene: 220904085

Centisome position: 33.05

GC content: 61.46

Gene sequence:

>672_bases
ATGGAAATCTGCCGCATCCTGCTTGTTGACGACCACAAGCTGCTTATGGAAGGCGTGCGCAGCCTGCTGGCCCCCTATGC
GCACTTGCGGGTAGTGGGCATGGCCCTTGCCGGTGGCGAGGCAGTGGCCCTGGCCGCCTCGCTGACGCCGCACCTGATGG
TGCTGGACCTCGGCATGCCCGGCATGAATGGGGTGGAAACCGCGCGCGCCGTGCTTGAAGTACGCCCGGCCACACGCATT
CTTGTCTATACCGGGCATGAGGATCAGCGCTGGCTGCCCGAGCTTATCGATCTTGGCATCATGGGGCATGTACGCAAATC
CGAGCCGCCGGGCGTGTTGTTGCGTGCCATCGAAAACGTGCGCCGGGGCGAGATATTTTTAAGTTTTTCTGATCCTGGCG
GGCGGCTGGCGGCCCTTTTGCGCGAACGCCGTCAGGCGGTGGACGAGTCCGGCGGCGATGGCTGCGGCGACCTGTCCGCT
CTGTCGCCCCGGGAAAAAGAGATTTTTCGCCTGCTGGCAGACGGGCACAGCGTCAAGGCCATCGCGGGCGACCTGCATAT
CAGCCCCAAAACCGTAGAAACCCACAAATATAATCTGCTCACCAAGTTACAGGCCGGGTCTGTGGGCGATCTGGTTAAAA
TTGCCATCCGTCACGGTCTGGTCAAAGTCTGA

Upstream 100 bases:

>100_bases
TTTTTCTGCTGTAGCCGCGCAGCCTGTCCGCACTGTTGAGGAGCGGCCGCCCCAGGGTCGCCCGGTCACCGCTTTGAACA
AAGGATGAGTGGACAGGCGT

Downstream 100 bases:

>100_bases
TCACACTCTGATCAAAGCCTGAATTTCCGTTTCACGCATTCGCGTTGATATTCCATCTTTCACAGCGCGCGGTACAGCCG
CGTTTTTTTGTGCCTTTTTT

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MEICRILLVDDHKLLMEGVRSLLAPYAHLRVVGMALAGGEAVALAASLTPHLMVLDLGMPGMNGVETARAVLEVRPATRI
LVYTGHEDQRWLPELIDLGIMGHVRKSEPPGVLLRAIENVRRGEIFLSFSDPGGRLAALLRERRQAVDESGGDGCGDLSA
LSPREKEIFRLLADGHSVKAIAGDLHISPKTVETHKYNLLTKLQAGSVGDLVKIAIRHGLVKV

Sequences:

>Translated_223_residues
MEICRILLVDDHKLLMEGVRSLLAPYAHLRVVGMALAGGEAVALAASLTPHLMVLDLGMPGMNGVETARAVLEVRPATRI
LVYTGHEDQRWLPELIDLGIMGHVRKSEPPGVLLRAIENVRRGEIFLSFSDPGGRLAALLRERRQAVDESGGDGCGDLSA
LSPREKEIFRLLADGHSVKAIAGDLHISPKTVETHKYNLLTKLQAGSVGDLVKIAIRHGLVKV
>Mature_223_residues
MEICRILLVDDHKLLMEGVRSLLAPYAHLRVVGMALAGGEAVALAASLTPHLMVLDLGMPGMNGVETARAVLEVRPATRI
LVYTGHEDQRWLPELIDLGIMGHVRKSEPPGVLLRAIENVRRGEIFLSFSDPGGRLAALLRERRQAVDESGGDGCGDLSA
LSPREKEIFRLLADGHSVKAIAGDLHISPKTVETHKYNLLTKLQAGSVGDLVKIAIRHGLVKV

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=218, Percent_Identity=29.8165137614679, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1788712, Length=214, Percent_Identity=27.5700934579439, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788521, Length=203, Percent_Identity=35.9605911330049, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787473, Length=195, Percent_Identity=36.9230769230769, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788546, Length=206, Percent_Identity=29.126213592233, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786747, Length=221, Percent_Identity=22.6244343891403, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24134; Mature: 24134

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEICRILLVDDHKLLMEGVRSLLAPYAHLRVVGMALAGGEAVALAASLTPHLMVLDLGMP
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCC
GMNGVETARAVLEVRPATRILVYTGHEDQRWLPELIDLGIMGHVRKSEPPGVLLRAIENV
CCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHC
RRGEIFLSFSDPGGRLAALLRERRQAVDESGGDGCGDLSALSPREKEIFRLLADGHSVKA
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
IAGDLHISPKTVETHKYNLLTKLQAGSVGDLVKIAIRHGLVKV
EECCEEECCCEECHHHHHHEEEECCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEICRILLVDDHKLLMEGVRSLLAPYAHLRVVGMALAGGEAVALAASLTPHLMVLDLGMP
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCC
GMNGVETARAVLEVRPATRILVYTGHEDQRWLPELIDLGIMGHVRKSEPPGVLLRAIENV
CCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHC
RRGEIFLSFSDPGGRLAALLRERRQAVDESGGDGCGDLSALSPREKEIFRLLADGHSVKA
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
IAGDLHISPKTVETHKYNLLTKLQAGSVGDLVKIAIRHGLVKV
EECCEEECCCEECHHHHHHEEEECCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]