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The map label for this gene is cobI [H]

Identifier: 220904073

GI number: 220904073

Start: 935807

End: 936517

Strand: Direct

Name: cobI [H]

Synonym: Ddes_0799

Alternate gene names: 220904073

Gene position: 935807-936517 (Clockwise)

Preceding gene: 220904072

Following gene: 220904083

Centisome position: 32.57

GC content: 61.04

Gene sequence:

>711_bases
ATGACAGGAGTTTTGTACGGCGTGGGCGTAGGGCCTGGCGCACCGGACCTGCTGACGCTGCGGGCTGTGAACGCCCTTGG
CAAGGTGGACGTGATTCTGGCGGCGGCTTCGCCCCGCAACGACTATTCCACCGCGTTGGAGACGGCCAGGCCGCACCTGC
GCCCGGATGTGCGCCTGCTGCGCCTGGAATTTCCCATGACGCGCGACCGCACCGTACTGCACGAGGCATGGCGCGTGGCG
GCCGAAGCCACGCAGCAAGTTCTGGAGGCCGGAGAAAACGCGGCCTTTCTGACCATTGGCGATCCGCTCGTATACAGCAC
TTTCGGCTATCTTATGCGCACGCTCAAAAAAAGCGCGCCGCACCTGCCGATAGAGATTATCCCCGGCATCACGTCCATTC
AGGCCGCCGCCGCATGTACGGGAACCATCCTGTGTGAAAACAGCGAAACCCTGAGCATCCTGCCGGGCATCAACAGCAGG
GAAGAACTTGAGCTGGCGCTGCACGGTGCTGATACGGCGGTTATTCTCAAGGCATACCGCAACCTTCCAGCCATTGTGGA
GGCGTTGCGCTCTACCGGACGGCTGGATTCATGCATTCTGGCAAGCCATGTGGAACAGCCTGCCCAGAATCTGCGCTTGG
GTGTCCAGGCCCAGGAAGGTACGCCCCCCTATATGTCATTGATTCTGAGCAGAAAGCCCAAGGCCGAATAG

Upstream 100 bases:

>100_bases
GCTGTGGACGCTGCCGAAGAACTGGGGCGGTTTTTGCATTTCCGGTCAGCCCCCTGAATTTTTCATAAACAATGCTGATG
ACACGACAAGAGAGCAGACC

Downstream 100 bases:

>100_bases
CACGGAGTAGGAAGGGGAAGGAAAAGGAAAGAGAAGGCTTTGGTGGGGGAAGGGGAATTTTTTTCAAAAAAATTCCCCTT
CCCCCACCAAAGCCTTCTCT

Product: precorrin-2 C20-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--precorrin-2 methyltransferase; SP2MT [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTGVLYGVGVGPGAPDLLTLRAVNALGKVDVILAAASPRNDYSTALETARPHLRPDVRLLRLEFPMTRDRTVLHEAWRVA
AEATQQVLEAGENAAFLTIGDPLVYSTFGYLMRTLKKSAPHLPIEIIPGITSIQAAAACTGTILCENSETLSILPGINSR
EELELALHGADTAVILKAYRNLPAIVEALRSTGRLDSCILASHVEQPAQNLRLGVQAQEGTPPYMSLILSRKPKAE

Sequences:

>Translated_236_residues
MTGVLYGVGVGPGAPDLLTLRAVNALGKVDVILAAASPRNDYSTALETARPHLRPDVRLLRLEFPMTRDRTVLHEAWRVA
AEATQQVLEAGENAAFLTIGDPLVYSTFGYLMRTLKKSAPHLPIEIIPGITSIQAAAACTGTILCENSETLSILPGINSR
EELELALHGADTAVILKAYRNLPAIVEALRSTGRLDSCILASHVEQPAQNLRLGVQAQEGTPPYMSLILSRKPKAE
>Mature_235_residues
TGVLYGVGVGPGAPDLLTLRAVNALGKVDVILAAASPRNDYSTALETARPHLRPDVRLLRLEFPMTRDRTVLHEAWRVAA
EATQQVLEAGENAAFLTIGDPLVYSTFGYLMRTLKKSAPHLPIEIIPGITSIQAAAACTGTILCENSETLSILPGINSRE
ELELALHGADTAVILKAYRNLPAIVEALRSTGRLDSCILASHVEQPAQNLRLGVQAQEGTPPYMSLILSRKPKAE

Specific function: Methylates precorrin-2 at the C-20 position to produce precorrin-3A [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.130 [H]

Molecular weight: Translated: 25351; Mature: 25219

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVLYGVGVGPGAPDLLTLRAVNALGKVDVILAAASPRNDYSTALETARPHLRPDVRLL
CCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
RLEFPMTRDRTVLHEAWRVAAEATQQVLEAGENAAFLTIGDPLVYSTFGYLMRTLKKSAP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCC
HLPIEIIPGITSIQAAAACTGTILCENSETLSILPGINSREELELALHGADTAVILKAYR
CCCEEECCCCHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHEEEEEECCCHHHHHHHHH
NLPAIVEALRSTGRLDSCILASHVEQPAQNLRLGVQAQEGTPPYMSLILSRKPKAE
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TGVLYGVGVGPGAPDLLTLRAVNALGKVDVILAAASPRNDYSTALETARPHLRPDVRLL
CCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
RLEFPMTRDRTVLHEAWRVAAEATQQVLEAGENAAFLTIGDPLVYSTFGYLMRTLKKSAP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCC
HLPIEIIPGITSIQAAAACTGTILCENSETLSILPGINSREELELALHGADTAVILKAYR
CCCEEECCCCHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHEEEEEECCCHHHHHHHHH
NLPAIVEALRSTGRLDSCILASHVEQPAQNLRLGVQAQEGTPPYMSLILSRKPKAE
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521 [H]