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The map label for this gene is lplA [H]

Identifier: 220904059

GI number: 220904059

Start: 919155

End: 920138

Strand: Direct

Name: lplA [H]

Synonym: Ddes_0785

Alternate gene names: 220904059

Gene position: 919155-920138 (Clockwise)

Preceding gene: 220904058

Following gene: 220904060

Centisome position: 31.99

GC content: 59.04

Gene sequence:

>984_bases
ATGGAACACCTTACTTTTTCTACCCTTGATCCCGCCTTTAACCTCGCGCTTGAAGAGGCGCTGTTTGAGACGCTTCCAGC
CGGTCATCCAGGGTATTTTCTGCTTTGGCAGAACGGCCCTTCGGTTATTGTGGGGCGCCACCAGTGTACGGTGGACGAAG
TCAATGCCGATTTCATCCGGCGTGAAAACCTGCCAGTGGTGCGTCGTATCACCGGCGGCGGGGCTGTGTATCATGATACG
GGCAATCTGAATTTTTCCTTTATTGAGCATGCCGGCGGCCGAGAAAAGGTGGATTTCCGGCGCTATCTTGAGCCGGTGTG
CGCGGCTCTGGCCGATCTCGGCGTGCGTGCCGGAATTTCCGGTCGCAATGACGTGGAGACCGAGGGACGCAAGATTTCCG
GCAGCGGGCAGATGCTGCGCCGGGGCAAGGTGCTGCACCACGGCACGCTGCTTATTGATGTGAACTTTGACCGCCTTGTG
GAAGCCCTGAATGTGGACCCGGAAAAGATGCGCTCCAAAGGGGTGGCGTCGGTGCGGTCACGGGTAGGCAATATCTCGGA
ATACTGGCAGCCGGGCAGCGATATGGACGCGCTTGTGGCTGCGCTGGTCAGGCGGTGCGCATCGCGACCCGGCAGTGTGG
GTGAAAGTATCATGGGCGAGGCGCACAGGCTTGCTGACGAAAAATACCGGCAGTGGAACTGGAACTACGGGGCCATGCCC
AACTTTACCGAGCGCAAGCGGCAGCGCTTCGACTGGGGCACGGTGGAACTGCGCCTTGATGTGAAGCAGGGGCGTATCGC
CTCCTGCGCCATTCTGGGAGATTTTTTCTGCAATGCCGGAGAAGGCATACAGGAACTGGAGCAAAGCCTCGCAGGGGCTT
CCCGGGAGCCTGAAGAGCTGGAAGCCATTTTGCGGCAACTGGACATGGGCCGCTACTTCAGCGGTTGCGAGGCCGAAACC
ATGACGGCATTTTTCACGAGCTGA

Upstream 100 bases:

>100_bases
CCAGGCGCACGGCCCGGAAATTTTGCCGATAATTTTGAAATTGCCCGCTGTTCCCTTGACCTTGTCACCTGAAGCGCGGC
ATTGTCCTGATGGAGGCCGC

Downstream 100 bases:

>100_bases
GGCGTAGTTGAGGGCGCGTCCGCCGCAGCCCTGCGACTGGTCCGGCCCATGCCGCCGAACCCCGTTTTTCCGTGCGGGCA
GGCCCGCGCGGCACGGCAGT

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MEHLTFSTLDPAFNLALEEALFETLPAGHPGYFLLWQNGPSVIVGRHQCTVDEVNADFIRRENLPVVRRITGGGAVYHDT
GNLNFSFIEHAGGREKVDFRRYLEPVCAALADLGVRAGISGRNDVETEGRKISGSGQMLRRGKVLHHGTLLIDVNFDRLV
EALNVDPEKMRSKGVASVRSRVGNISEYWQPGSDMDALVAALVRRCASRPGSVGESIMGEAHRLADEKYRQWNWNYGAMP
NFTERKRQRFDWGTVELRLDVKQGRIASCAILGDFFCNAGEGIQELEQSLAGASREPEELEAILRQLDMGRYFSGCEAET
MTAFFTS

Sequences:

>Translated_327_residues
MEHLTFSTLDPAFNLALEEALFETLPAGHPGYFLLWQNGPSVIVGRHQCTVDEVNADFIRRENLPVVRRITGGGAVYHDT
GNLNFSFIEHAGGREKVDFRRYLEPVCAALADLGVRAGISGRNDVETEGRKISGSGQMLRRGKVLHHGTLLIDVNFDRLV
EALNVDPEKMRSKGVASVRSRVGNISEYWQPGSDMDALVAALVRRCASRPGSVGESIMGEAHRLADEKYRQWNWNYGAMP
NFTERKRQRFDWGTVELRLDVKQGRIASCAILGDFFCNAGEGIQELEQSLAGASREPEELEAILRQLDMGRYFSGCEAET
MTAFFTS
>Mature_327_residues
MEHLTFSTLDPAFNLALEEALFETLPAGHPGYFLLWQNGPSVIVGRHQCTVDEVNADFIRRENLPVVRRITGGGAVYHDT
GNLNFSFIEHAGGREKVDFRRYLEPVCAALADLGVRAGISGRNDVETEGRKISGSGQMLRRGKVLHHGTLLIDVNFDRLV
EALNVDPEKMRSKGVASVRSRVGNISEYWQPGSDMDALVAALVRRCASRPGSVGESIMGEAHRLADEKYRQWNWNYGAMP
NFTERKRQRFDWGTVELRLDVKQGRIASCAILGDFFCNAGEGIQELEQSLAGASREPEELEAILRQLDMGRYFSGCEAET
MTAFFTS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=301, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI21729884, Length=301, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI21729882, Length=301, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI21729880, Length=301, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI21729878, Length=301, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1790846, Length=283, Percent_Identity=36.3957597173145, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71981909, Length=243, Percent_Identity=31.2757201646091, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322415, Length=280, Percent_Identity=27.1428571428571, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573464, Length=271, Percent_Identity=30.6273062730627, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 36430; Mature: 36430

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLTFSTLDPAFNLALEEALFETLPAGHPGYFLLWQNGPSVIVGRHQCTVDEVNADFIR
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCHHHCCHHHHH
RENLPVVRRITGGGAVYHDTGNLNFSFIEHAGGREKVDFRRYLEPVCAALADLGVRAGIS
HCCCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GRNDVETEGRKISGSGQMLRRGKVLHHGTLLIDVNFDRLVEALNVDPEKMRSKGVASVRS
CCCCCCCCCCEECCCCHHHHCCCEEECCEEEEEECHHHHHHHHCCCHHHHHHCCHHHHHH
RVGNISEYWQPGSDMDALVAALVRRCASRPGSVGESIMGEAHRLADEKYRQWNWNYGAMP
HHCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NFTERKRQRFDWGTVELRLDVKQGRIASCAILGDFFCNAGEGIQELEQSLAGASREPEEL
CHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
EAILRQLDMGRYFSGCEAETMTAFFTS
HHHHHHHHCCHHCCCCCHHHHHHEECC
>Mature Secondary Structure
MEHLTFSTLDPAFNLALEEALFETLPAGHPGYFLLWQNGPSVIVGRHQCTVDEVNADFIR
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCHHHCCHHHHH
RENLPVVRRITGGGAVYHDTGNLNFSFIEHAGGREKVDFRRYLEPVCAALADLGVRAGIS
HCCCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GRNDVETEGRKISGSGQMLRRGKVLHHGTLLIDVNFDRLVEALNVDPEKMRSKGVASVRS
CCCCCCCCCCEECCCCHHHHCCCEEECCEEEEEECHHHHHHHHCCCHHHHHHCCHHHHHH
RVGNISEYWQPGSDMDALVAALVRRCASRPGSVGESIMGEAHRLADEKYRQWNWNYGAMP
HHCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NFTERKRQRFDWGTVELRLDVKQGRIASCAILGDFFCNAGEGIQELEQSLAGASREPEEL
CHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
EAILRQLDMGRYFSGCEAETMTAFFTS
HHHHHHHHCCHHCCCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]