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The map label for this gene is pstS [H]

Identifier: 220904055

GI number: 220904055

Start: 915507

End: 916343

Strand: Direct

Name: pstS [H]

Synonym: Ddes_0781

Alternate gene names: 220904055

Gene position: 915507-916343 (Clockwise)

Preceding gene: 220904054

Following gene: 220904056

Centisome position: 31.86

GC content: 57.35

Gene sequence:

>837_bases
ATGAAACGTATAATTCTTGCGGCTGTTCTGCTTATGTGCGGCCTCGGCACGGCCTTTGCTGCCTCGCCTTCCGCCCCTCT
GGATGCATTCAGGGGGCAGAAGGGAGTTGTGGATATTGCGGGAGGTACGGCCCACATTCCTGTTATGAAAGAGGCCGCCA
GGCAGATCATGGCCGTTAACCCGGACGTACGCATTACTGTGGCAGGGGGCGGCTCCGGCGTGGGTGTGCAGAAAGTGGGC
GAGGGCATTGTGCAGATCGGCAATACCGGCCGCGCCCTCAAACCTGCGGAAGTGGAAAAATACGGTCTTGTGACCTTTCC
ATTTGCCATTGACGGCGTGGCTGTGGCTGTCAATCCGGCCAACAGGGTCACGGGGCTGACCAAAGCCCAGATAAAGGATG
TTTTTTCCGGCAAAATAGCCAACTGGAAGGAAGTGGGCGGTGCTGACGCTCCCATATCTCTCTATGTGCGCGAAGACGGC
AGCGGCACGCGCGAAACGTTTGAAGAACGCGCCCTGGACAAAGGCGTCGCAGCGTCCAAAGCCAACGTGGTCAGTTCCAA
CGGGGCCATGAAAACAGCTGTCAGCCAGGATCCCAACGCCATCGGCTATGTGGGCATCGGCCATCTGGACAACAGCATCA
AGGGCATAAGCATTGACGGTGCCACTCCCAGTCAGGAAAACGCCGCTTCAGGCGAGTACAAGGTTACGCGCCTTCTGTAC
ATGAACACCAAGGGCGAACCGCAAGGCCTGACCAAGGCCTTTGTGGAATATATCTTCACTCCCGCCGGGCAGGATATCGT
ATCCGGTGCCGGCTACATCCCCTATGTCAAGAAATAG

Upstream 100 bases:

>100_bases
TCCATCTTCTACCTGACTCCCGGCGATGTAATCGCGTCTGTTGCTGTTCACTGCGTGGCGCGTCCGCGCTTTCTGTTCAC
GCTAACCGTAGGAGACTGTC

Downstream 100 bases:

>100_bases
GAATCGCTCCGCCCGCCAGGCCGGGGGCGCTGCGGTGCTTCCGGCTGCGGGCCGCCATATGCGGGATAATACCGACAGCC
CCGGCCGCCTTCTGCTGCTG

Product: phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKRIILAAVLLMCGLGTAFAASPSAPLDAFRGQKGVVDIAGGTAHIPVMKEAARQIMAVNPDVRITVAGGGSGVGVQKVG
EGIVQIGNTGRALKPAEVEKYGLVTFPFAIDGVAVAVNPANRVTGLTKAQIKDVFSGKIANWKEVGGADAPISLYVREDG
SGTRETFEERALDKGVAASKANVVSSNGAMKTAVSQDPNAIGYVGIGHLDNSIKGISIDGATPSQENAASGEYKVTRLLY
MNTKGEPQGLTKAFVEYIFTPAGQDIVSGAGYIPYVKK

Sequences:

>Translated_278_residues
MKRIILAAVLLMCGLGTAFAASPSAPLDAFRGQKGVVDIAGGTAHIPVMKEAARQIMAVNPDVRITVAGGGSGVGVQKVG
EGIVQIGNTGRALKPAEVEKYGLVTFPFAIDGVAVAVNPANRVTGLTKAQIKDVFSGKIANWKEVGGADAPISLYVREDG
SGTRETFEERALDKGVAASKANVVSSNGAMKTAVSQDPNAIGYVGIGHLDNSIKGISIDGATPSQENAASGEYKVTRLLY
MNTKGEPQGLTKAFVEYIFTPAGQDIVSGAGYIPYVKK
>Mature_278_residues
MKRIILAAVLLMCGLGTAFAASPSAPLDAFRGQKGVVDIAGGTAHIPVMKEAARQIMAVNPDVRITVAGGGSGVGVQKVG
EGIVQIGNTGRALKPAEVEKYGLVTFPFAIDGVAVAVNPANRVTGLTKAQIKDVFSGKIANWKEVGGADAPISLYVREDG
SGTRETFEERALDKGVAASKANVVSSNGAMKTAVSQDPNAIGYVGIGHLDNSIKGISIDGATPSQENAASGEYKVTRLLY
MNTKGEPQGLTKAFVEYIFTPAGQDIVSGAGYIPYVKK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 28737; Mature: 28737

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIILAAVLLMCGLGTAFAASPSAPLDAFRGQKGVVDIAGGTAHIPVMKEAARQIMAVN
CHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCCEEEECCCCEECHHHHHHHHHHEEEC
PDVRITVAGGGSGVGVQKVGEGIVQIGNTGRALKPAEVEKYGLVTFPFAIDGVAVAVNPA
CCEEEEEECCCCCCCHHHHCCHHEEECCCCCCCCCCCHHHCCEEEECEEECCEEEEECCH
NRVTGLTKAQIKDVFSGKIANWKEVGGADAPISLYVREDGSGTRETFEERALDKGVAASK
HHHCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
ANVVSSNGAMKTAVSQDPNAIGYVGIGHLDNSIKGISIDGATPSQENAASGEYKVTRLLY
CEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEE
MNTKGEPQGLTKAFVEYIFTPAGQDIVSGAGYIPYVKK
ECCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRIILAAVLLMCGLGTAFAASPSAPLDAFRGQKGVVDIAGGTAHIPVMKEAARQIMAVN
CHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCCEEEECCCCEECHHHHHHHHHHEEEC
PDVRITVAGGGSGVGVQKVGEGIVQIGNTGRALKPAEVEKYGLVTFPFAIDGVAVAVNPA
CCEEEEEECCCCCCCHHHHCCHHEEECCCCCCCCCCCHHHCCEEEECEEECCEEEEECCH
NRVTGLTKAQIKDVFSGKIANWKEVGGADAPISLYVREDGSGTRETFEERALDKGVAASK
HHHCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
ANVVSSNGAMKTAVSQDPNAIGYVGIGHLDNSIKGISIDGATPSQENAASGEYKVTRLLY
CEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEE
MNTKGEPQGLTKAFVEYIFTPAGQDIVSGAGYIPYVKK
ECCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]