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The map label for this gene is hyfF [H]

Identifier: 220904049

GI number: 220904049

Start: 907902

End: 909395

Strand: Reverse

Name: hyfF [H]

Synonym: Ddes_0775

Alternate gene names: 220904049

Gene position: 909395-907902 (Counterclockwise)

Preceding gene: 220904050

Following gene: 220904048

Centisome position: 31.65

GC content: 61.18

Gene sequence:

>1494_bases
ATGCTGGAAGCCTTGCTCTTTCTCCCGCTGGCGGCGGGCTGTATCATGCTGCTTGGCACAAGTGCCCTGTGCCGCATTCT
GCTGCCCCTGACAGGACTGACCCACGCCGGGCTGGCCGCCTGGACCACCTGGAACGTGGCCAGCGGGGCCGAACCGGAAG
CCTTGGGCGGCCTGCTGGCGCCGGACCCGCTGGGCGCGCTTTTTCTCATGCTGGCCAGCCTGCTCTTTCTGGCGGCCTCC
GTGTACGCTGTGTATTATCTGCGTGAAGAAGAAAGCCTCGGTGTGCATACTGCCTTTCAGGACGGGCGCAGCTTCACCAA
TGCGCCGCAGCGCCGTCTGGCCGCCTGCCTGTGCTTTTTTCTTGCATCAATGACGCTGGTAACCACCACTCCGCACCTTG
GGGCATTATGGGTGGGCATTGAAGCCACCACGCTTTCCAGCGCGCCGCTGATCTATTTTCACCGCCACCAACGCTCCCTT
GAGGCCACCTGGAAATACCTGATCATATGCTCCGTGGGCATTGCCCTGGCCATGGTGGGCAATATTCTGCTTTCTGTGGC
TTTTTACACGCCTGAAGGCATGGAAGCTATGGTGGAAGGCATGAACCAGGTGGGCTGGTATGTCAGCCACGCCCGCGCCG
GAGAAGAAACCTGGCTCAAGGCGGCGTTTATTTTCCTGCTGGTGGGGTACGGCACAAAGATGGGTCTGGCCCCCCTGCAC
AACTGGCTGCCCGATGCCCACAGTCAGGCCCCTTCGCTGGTCTCGGCCCTGCTGTCCGGCGCACTGCTCAACTGTGCCAT
GCTCGGCATACTGCGCGGCCACCAGATCATGCTGGCCGCCGGGCTTGGCGGCTTCAGCAGCGGCCTGCTCAGCTTTTTCG
GTCTGCTGTCGCTGATTACGGCGGCCATCTTCATTGTGGGCCAGGGGCATTACAAGCGCATGCTGGCCTATTCCAGCGTG
GAGCATATGGGCATACTGGCTCTTGGAGTGGGCGTTGGTGGCCTGGCAGTGTCGGGAGCCATGCTGCACGCGGTCTGCCA
TTCGCTGACCAAGTGCATGCTCTTTTTACTTTCCGGCAATATCCTGGCCCGTTATCACACATTTTCGAGCTACGACATTC
GCGGCATGCGCTGGACCATGCCAGTTACAGGCGCCCTGTGGATGGCCGGATTTCTGGCAGTGGCCGGTTCGCCGCCTTTC
GGCATTTTCATCAGCGAATTCATTATATTCAAAGGAATGCTTGCTGCCGGATCCCCTTTTCTGGCCGCCGCCTATCTGCT
GGCTCTGGCCGTAATATTTGTGGGCCTTTCGGTAGCGGTGCTGCGCATGGTGCAAGGCAGCCGCCCCACAGACATGCCCC
AGAATCCGCGCGAGCCGCTGCTGAGCGTGCTTCCGCCCCTGGCGCTCGGCATGGGCGTACTGACACTGGGCCTGTGGATA
CCCGACTGGCTGTGGAATTTTTTGCGGCAGGGCGCGGCCCTTATCGGCGGCTAG

Upstream 100 bases:

>100_bases
TTGTGGCTGTCTTTGTTATGGGCATTGCCATCAACCACATCAGCAACACCTTTGAATCCATTGATGTAGGCCGCTTTCGC
AGCCTCAGGGATTGACGGCC

Downstream 100 bases:

>100_bases
AGCAGTGTTGTTTTAACATTGTTCCGGCGGCTGCATGAGCAGGCGTCCGCTACGAAGGTGTAGGTGCAGCGTATTTGCAC
TGCCAAACACCGCAGCGAGC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLEALLFLPLAAGCIMLLGTSALCRILLPLTGLTHAGLAAWTTWNVASGAEPEALGGLLAPDPLGALFLMLASLLFLAAS
VYAVYYLREEESLGVHTAFQDGRSFTNAPQRRLAACLCFFLASMTLVTTTPHLGALWVGIEATTLSSAPLIYFHRHQRSL
EATWKYLIICSVGIALAMVGNILLSVAFYTPEGMEAMVEGMNQVGWYVSHARAGEETWLKAAFIFLLVGYGTKMGLAPLH
NWLPDAHSQAPSLVSALLSGALLNCAMLGILRGHQIMLAAGLGGFSSGLLSFFGLLSLITAAIFIVGQGHYKRMLAYSSV
EHMGILALGVGVGGLAVSGAMLHAVCHSLTKCMLFLLSGNILARYHTFSSYDIRGMRWTMPVTGALWMAGFLAVAGSPPF
GIFISEFIIFKGMLAAGSPFLAAAYLLALAVIFVGLSVAVLRMVQGSRPTDMPQNPREPLLSVLPPLALGMGVLTLGLWI
PDWLWNFLRQGAALIGG

Sequences:

>Translated_497_residues
MLEALLFLPLAAGCIMLLGTSALCRILLPLTGLTHAGLAAWTTWNVASGAEPEALGGLLAPDPLGALFLMLASLLFLAAS
VYAVYYLREEESLGVHTAFQDGRSFTNAPQRRLAACLCFFLASMTLVTTTPHLGALWVGIEATTLSSAPLIYFHRHQRSL
EATWKYLIICSVGIALAMVGNILLSVAFYTPEGMEAMVEGMNQVGWYVSHARAGEETWLKAAFIFLLVGYGTKMGLAPLH
NWLPDAHSQAPSLVSALLSGALLNCAMLGILRGHQIMLAAGLGGFSSGLLSFFGLLSLITAAIFIVGQGHYKRMLAYSSV
EHMGILALGVGVGGLAVSGAMLHAVCHSLTKCMLFLLSGNILARYHTFSSYDIRGMRWTMPVTGALWMAGFLAVAGSPPF
GIFISEFIIFKGMLAAGSPFLAAAYLLALAVIFVGLSVAVLRMVQGSRPTDMPQNPREPLLSVLPPLALGMGVLTLGLWI
PDWLWNFLRQGAALIGG
>Mature_497_residues
MLEALLFLPLAAGCIMLLGTSALCRILLPLTGLTHAGLAAWTTWNVASGAEPEALGGLLAPDPLGALFLMLASLLFLAAS
VYAVYYLREEESLGVHTAFQDGRSFTNAPQRRLAACLCFFLASMTLVTTTPHLGALWVGIEATTLSSAPLIYFHRHQRSL
EATWKYLIICSVGIALAMVGNILLSVAFYTPEGMEAMVEGMNQVGWYVSHARAGEETWLKAAFIFLLVGYGTKMGLAPLH
NWLPDAHSQAPSLVSALLSGALLNCAMLGILRGHQIMLAAGLGGFSSGLLSFFGLLSLITAAIFIVGQGHYKRMLAYSSV
EHMGILALGVGVGGLAVSGAMLHAVCHSLTKCMLFLLSGNILARYHTFSSYDIRGMRWTMPVTGALWMAGFLAVAGSPPF
GIFISEFIIFKGMLAAGSPFLAAAYLLALAVIFVGLSVAVLRMVQGSRPTDMPQNPREPLLSVLPPLALGMGVLTLGLWI
PDWLWNFLRQGAALIGG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=383, Percent_Identity=41.5143603133159, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788827, Length=388, Percent_Identity=29.8969072164948, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788613, Length=198, Percent_Identity=34.8484848484849, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=293, Percent_Identity=28.3276450511945, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788829, Length=357, Percent_Identity=25.2100840336134, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788614, Length=341, Percent_Identity=26.6862170087977, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI2367154, Length=210, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52917; Mature: 52917

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEALLFLPLAAGCIMLLGTSALCRILLPLTGLTHAGLAAWTTWNVASGAEPEALGGLLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCHHHHCCCCC
PDPLGALFLMLASLLFLAASVYAVYYLREEESLGVHTAFQDGRSFTNAPQRRLAACLCFF
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
LASMTLVTTTPHLGALWVGIEATTLSSAPLIYFHRHQRSLEATWKYLIICSVGIALAMVG
HHHHHHHCCCCCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
NILLSVAFYTPEGMEAMVEGMNQVGWYVSHARAGEETWLKAAFIFLLVGYGTKMGLAPLH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
NWLPDAHSQAPSLVSALLSGALLNCAMLGILRGHQIMLAAGLGGFSSGLLSFFGLLSLIT
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AAIFIVGQGHYKRMLAYSSVEHMGILALGVGVGGLAVSGAMLHAVCHSLTKCMLFLLSGN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILARYHTFSSYDIRGMRWTMPVTGALWMAGFLAVAGSPPFGIFISEFIIFKGMLAAGSPF
HHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
LAAAYLLALAVIFVGLSVAVLRMVQGSRPTDMPQNPREPLLSVLPPLALGMGVLTLGLWI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PDWLWNFLRQGAALIGG
HHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MLEALLFLPLAAGCIMLLGTSALCRILLPLTGLTHAGLAAWTTWNVASGAEPEALGGLLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCHHHHCCCCC
PDPLGALFLMLASLLFLAASVYAVYYLREEESLGVHTAFQDGRSFTNAPQRRLAACLCFF
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
LASMTLVTTTPHLGALWVGIEATTLSSAPLIYFHRHQRSLEATWKYLIICSVGIALAMVG
HHHHHHHCCCCCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
NILLSVAFYTPEGMEAMVEGMNQVGWYVSHARAGEETWLKAAFIFLLVGYGTKMGLAPLH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
NWLPDAHSQAPSLVSALLSGALLNCAMLGILRGHQIMLAAGLGGFSSGLLSFFGLLSLIT
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AAIFIVGQGHYKRMLAYSSVEHMGILALGVGVGGLAVSGAMLHAVCHSLTKCMLFLLSGN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILARYHTFSSYDIRGMRWTMPVTGALWMAGFLAVAGSPPFGIFISEFIIFKGMLAAGSPF
HHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
LAAAYLLALAVIFVGLSVAVLRMVQGSRPTDMPQNPREPLLSVLPPLALGMGVLTLGLWI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PDWLWNFLRQGAALIGG
HHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]