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The map label for this gene is hyfG [H]

Identifier: 220904048

GI number: 220904048

Start: 906047

End: 907744

Strand: Reverse

Name: hyfG [H]

Synonym: Ddes_0774

Alternate gene names: 220904048

Gene position: 907744-906047 (Counterclockwise)

Preceding gene: 220904049

Following gene: 220904047

Centisome position: 31.59

GC content: 62.78

Gene sequence:

>1698_bases
ATGTCGTTCGACTACCGTGAGGCGGTAACCCTGAACAAACTGGGGCGTCATTCCGTATCTGAACTGCGGGACATTCTCCG
GCACGCCCTGGCCGGTGGATGGCGCGTACTGGCCTTTTTCGGCATTCCTGACATGGATGCCCCCCTTGACGCGCACAACG
ATGACACACCGGAACAGCCCCGTGCCGTAAGCCTTTGCTGCATACTGGCCCATGACGGTACGCGCCGCCTTATGGCCCGA
TGCACGCCGCCGGTGACGGCCTTTGTATCAATGACGCCGGAATTGCCGCAACTGCACTATTTCGAGCGCGAGATTTACGA
ACAGTGGGGCGTGGAGCCGGTGGGGCATCCCTGGCTCAAAAGTGTGCGGCGCATCCCCGACAGCGCCGAAGCCGCAGCGC
GCAATGCCGCGGAAAGCCAAACCAGCAGCGCCGCCCCTGCCCAGGAATCTGCGGAAGTAGTTCCTTCTCCGGCCTTGACG
GCTTCTGCACGGCCCCAGACACTGCCATATCCCTTTTACCGGGTAGAAGGGGCAGATGTACACGAAGTTGCCGTGGGGCC
TGTACATGCGGGCATCATCGAACCCGGGCATTTTCGTTTTCAGTGCTACGGCGAGAATGTGTTGCATCTGGAAATAGCGC
TGGGCTACCAGCATCGGGGCCTTGAGGCCATGATCACTCAAGGGCCTGCCGGCCGTCGCCTGCACCTGCTGGAATGCGCC
GCTGGCGACAGCACCGTTGCCCATGCCACGGCCCACTGTGTGCTGCTTGAGCGCATGACCGGGCAACAGCCTGATGAACG
CGCCCAGCTGTTGCGCCGCATTGGCCTTGAGCTGGAACGCCTCGCCAACCACACGGGCGACCTTGGAGCCATTGCGGGCG
ATACGGGCTTTTTGCCCACTGCCTCATGGAATGGCCGCATCCGCGGCGATTTTCTCAATATAACCGCCAGCCTGTGCGGG
AACCGTTTCGGCAGGGGGCTGCTGCGCCCGGGGGGCGTGGCCTACGACCTTGCCCCATCGGAATGTGCTGATATGCTCGC
CCGCGTTCGTGCGGCCTGGCACGATGCGCGCGGTTCGGTGGACGTGATGCTTGATGCCGTGACCGTGCGTAACCGCCTTG
CGGATACCGGCATGGTGGAACCTGCCACAGCCCGTGAGCTGGGTCTTGTGGGTGTTGCCGCACGTGCCTGCGGGCTGGAC
GTGGATGCGCGTTTTCACATGCCCCTCAGCGACCTGCCTTGCGAAGGATGCGCGCCCCGGCTGGAAATAACGGGCGACGT
CATGGCCCGCACCCTTGTGCGCAGCCGCGAACTGGACGACAGTCTGCGCCTGCTGGAGTTTGACCTTGCCCGGCTGGGGG
CGTTGCCCCACACAAAAAATGCAGCAACGGCGGAAAGTACGGTTTCCGCACCCGCCACTGCCGCAGCAACCAATACTTCC
GGCACAGCGGCTGAGGCCGCCTCTTTTGATGCTCTGCCGCCGGACACCCTGGCGGTGGCACTGGTAGAAGGCTGGCGCGG
CGAAGTATGCCATGTGGGGCTTACTGACTCCCGGGGGCGCCTGCTGGTCTACAAGGTTGTCGACCCTTCTTTCCATAACT
GGGCAGGGCTGGCCATGGCCCTGCGTGGCAACCAGATTTCAGACTTTCCCCTCTGCAACAAGAGCTTCAACCTCTCTTAT
TGCGGGCATGACCTGTGA

Upstream 100 bases:

>100_bases
GTGTAGGTGCAGCGTATTTGCACTGCCAAACACCGCAGCGAGCATTTGAAACCTTTGAGAACATGTATTCGCAAAGATAA
AATGCTCCAGGAGGATCTTG

Downstream 100 bases:

>100_bases
GGTTTTGACATGTTGCGCATCATCAAGGAACGCATTCATCAGAAATACCGCACGCTGGACTATCCACACCAACAGCCCAC
ACTCTCGCCCCGGTACATGG

Product: NAD-dependent dehydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSFDYREAVTLNKLGRHSVSELRDILRHALAGGWRVLAFFGIPDMDAPLDAHNDDTPEQPRAVSLCCILAHDGTRRLMAR
CTPPVTAFVSMTPELPQLHYFEREIYEQWGVEPVGHPWLKSVRRIPDSAEAAARNAAESQTSSAAPAQESAEVVPSPALT
ASARPQTLPYPFYRVEGADVHEVAVGPVHAGIIEPGHFRFQCYGENVLHLEIALGYQHRGLEAMITQGPAGRRLHLLECA
AGDSTVAHATAHCVLLERMTGQQPDERAQLLRRIGLELERLANHTGDLGAIAGDTGFLPTASWNGRIRGDFLNITASLCG
NRFGRGLLRPGGVAYDLAPSECADMLARVRAAWHDARGSVDVMLDAVTVRNRLADTGMVEPATARELGLVGVAARACGLD
VDARFHMPLSDLPCEGCAPRLEITGDVMARTLVRSRELDDSLRLLEFDLARLGALPHTKNAATAESTVSAPATAAATNTS
GTAAEAASFDALPPDTLAVALVEGWRGEVCHVGLTDSRGRLLVYKVVDPSFHNWAGLAMALRGNQISDFPLCNKSFNLSY
CGHDL

Sequences:

>Translated_565_residues
MSFDYREAVTLNKLGRHSVSELRDILRHALAGGWRVLAFFGIPDMDAPLDAHNDDTPEQPRAVSLCCILAHDGTRRLMAR
CTPPVTAFVSMTPELPQLHYFEREIYEQWGVEPVGHPWLKSVRRIPDSAEAAARNAAESQTSSAAPAQESAEVVPSPALT
ASARPQTLPYPFYRVEGADVHEVAVGPVHAGIIEPGHFRFQCYGENVLHLEIALGYQHRGLEAMITQGPAGRRLHLLECA
AGDSTVAHATAHCVLLERMTGQQPDERAQLLRRIGLELERLANHTGDLGAIAGDTGFLPTASWNGRIRGDFLNITASLCG
NRFGRGLLRPGGVAYDLAPSECADMLARVRAAWHDARGSVDVMLDAVTVRNRLADTGMVEPATARELGLVGVAARACGLD
VDARFHMPLSDLPCEGCAPRLEITGDVMARTLVRSRELDDSLRLLEFDLARLGALPHTKNAATAESTVSAPATAAATNTS
GTAAEAASFDALPPDTLAVALVEGWRGEVCHVGLTDSRGRLLVYKVVDPSFHNWAGLAMALRGNQISDFPLCNKSFNLSY
CGHDL
>Mature_564_residues
SFDYREAVTLNKLGRHSVSELRDILRHALAGGWRVLAFFGIPDMDAPLDAHNDDTPEQPRAVSLCCILAHDGTRRLMARC
TPPVTAFVSMTPELPQLHYFEREIYEQWGVEPVGHPWLKSVRRIPDSAEAAARNAAESQTSSAAPAQESAEVVPSPALTA
SARPQTLPYPFYRVEGADVHEVAVGPVHAGIIEPGHFRFQCYGENVLHLEIALGYQHRGLEAMITQGPAGRRLHLLECAA
GDSTVAHATAHCVLLERMTGQQPDERAQLLRRIGLELERLANHTGDLGAIAGDTGFLPTASWNGRIRGDFLNITASLCGN
RFGRGLLRPGGVAYDLAPSECADMLARVRAAWHDARGSVDVMLDAVTVRNRLADTGMVEPATARELGLVGVAARACGLDV
DARFHMPLSDLPCEGCAPRLEITGDVMARTLVRSRELDDSLRLLEFDLARLGALPHTKNAATAESTVSAPATAAATNTSG
TAAEAASFDALPPDTLAVALVEGWRGEVCHVGLTDSRGRLLVYKVVDPSFHNWAGLAMALRGNQISDFPLCNKSFNLSYC
GHDL

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=349, Percent_Identity=23.7822349570201, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI4758786, Length=349, Percent_Identity=23.7822349570201, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788832, Length=491, Percent_Identity=27.9022403258656, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789076, Length=493, Percent_Identity=27.1805273833671, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI145693162, Length=561, Percent_Identity=21.9251336898396, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568379, Length=251, Percent_Identity=24.3027888446215, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221459469, Length=264, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24638644, Length=350, Percent_Identity=23.4285714285714, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 60968; Mature: 60837

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDYREAVTLNKLGRHSVSELRDILRHALAGGWRVLAFFGIPDMDAPLDAHNDDTPEQP
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
RAVSLCCILAHDGTRRLMARCTPPVTAFVSMTPELPQLHYFEREIYEQWGVEPVGHPWLK
CEEEEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
SVRRIPDSAEAAARNAAESQTSSAAPAQESAEVVPSPALTASARPQTLPYPFYRVEGADV
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHCHHCCCCCCCCCCCCCCCCCCCEEEECCCCE
HEVAVGPVHAGIIEPGHFRFQCYGENVLHLEIALGYQHRGLEAMITQGPAGRRLHLLECA
EEEEECCEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCEEEEECCCCCCEEEEEEEC
AGDSTVAHATAHCVLLERMTGQQPDERAQLLRRIGLELERLANHTGDLGAIAGDTGFLPT
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHCCCCCCCEECCCCCCCCC
ASWNGRIRGDFLNITASLCGNRFGRGLLRPGGVAYDLAPSECADMLARVRAAWHDARGSV
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCE
DVMLDAVTVRNRLADTGMVEPATARELGLVGVAARACGLDVDARFHMPLSDLPCEGCAPR
EEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCC
LEITGDVMARTLVRSRELDDSLRLLEFDLARLGALPHTKNAATAESTVSAPATAAATNTS
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHCCCCCC
GTAAEAASFDALPPDTLAVALVEGWRGEVCHVGLTDSRGRLLVYKVVDPSFHNWAGLAMA
CCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHEEEE
LRGNQISDFPLCNKSFNLSYCGHDL
EECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFDYREAVTLNKLGRHSVSELRDILRHALAGGWRVLAFFGIPDMDAPLDAHNDDTPEQP
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
RAVSLCCILAHDGTRRLMARCTPPVTAFVSMTPELPQLHYFEREIYEQWGVEPVGHPWLK
CEEEEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
SVRRIPDSAEAAARNAAESQTSSAAPAQESAEVVPSPALTASARPQTLPYPFYRVEGADV
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHCHHCCCCCCCCCCCCCCCCCCCEEEECCCCE
HEVAVGPVHAGIIEPGHFRFQCYGENVLHLEIALGYQHRGLEAMITQGPAGRRLHLLECA
EEEEECCEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCEEEEECCCCCCEEEEEEEC
AGDSTVAHATAHCVLLERMTGQQPDERAQLLRRIGLELERLANHTGDLGAIAGDTGFLPT
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHCCCCCCCEECCCCCCCCC
ASWNGRIRGDFLNITASLCGNRFGRGLLRPGGVAYDLAPSECADMLARVRAAWHDARGSV
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCE
DVMLDAVTVRNRLADTGMVEPATARELGLVGVAARACGLDVDARFHMPLSDLPCEGCAPR
EEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCC
LEITGDVMARTLVRSRELDDSLRLLEFDLARLGALPHTKNAATAESTVSAPATAAATNTS
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHCCCCCC
GTAAEAASFDALPPDTLAVALVEGWRGEVCHVGLTDSRGRLLVYKVVDPSFHNWAGLAMA
CCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHEEEE
LRGNQISDFPLCNKSFNLSYCGHDL
EECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]