Click here to switch to the map view.

The map label for this gene is hyfI [H]

Identifier: 220904047

GI number: 220904047

Start: 905273

End: 906037

Strand: Reverse

Name: hyfI [H]

Synonym: Ddes_0773

Alternate gene names: 220904047

Gene position: 906037-905273 (Counterclockwise)

Preceding gene: 220904048

Following gene: 220904046

Centisome position: 31.53

GC content: 59.61

Gene sequence:

>765_bases
ATGTTGCGCATCATCAAGGAACGCATTCATCAGAAATACCGCACGCTGGACTATCCACACCAACAGCCCACACTCTCGCC
CCGGTACATGGGCCGTCCAAGCCTGATCCCTGTGGATTGCGGTGACTGCCGCGCCTGTTATGCGGCCTGCCCCACTGCCG
CCCTGTTGCCCACAGAGGGCGTACCCGGCAGTACGCCAACACTGGATATGGGCCGCTGCACCTTCTGCGGAGCCTGCCGC
GCTGCCTGCCCCAAGCAGGCCATCACGTTTACGGGTGAACACCGCATGGCGGCCTTCAGGCGCGAAGATCTGCTGATAGT
ACCCGGAAAACCTGTGACGCAGCCCGCAACGCCCCCTGTGCCGGGTAAATTTGCCATATTCAGGCGTTCCCTCAAGCTGC
GTCAGGTGAGCGCTGCGGGCTGTAACGCCTGCGAAGCCGATTGTAACGTGCTCGGAACCCTTGTGTTTGACCTGCCGCGC
TTCGGTATTGATTTTGTCGCTTCGCCACGACACGCCGACGGCATCGTGCTTACCGGTCCTGTTTCTGAAAACATGCGGCT
GGCAGCTATTGATACATATAACGCCACGCCGGAACCACGCCTCGTGGTGGCTGTGGGGGCCTGCGCCATATCGGGCGGGC
TTTTCCGCGATGCCCCACAGTGTAATAACGGCATTGCAGATATTTTGCCCGTGGACCTTTTTGTTCCGGGCTGCCCACCC
AACCCCTGGACTATTCTGGACGGTTTGCTAATGTTGCGGGAATAA

Upstream 100 bases:

>100_bases
GCTGGCCATGGCCCTGCGTGGCAACCAGATTTCAGACTTTCCCCTCTGCAACAAGAGCTTCAACCTCTCTTATTGCGGGC
ATGACCTGTGAGGTTTTGAC

Downstream 100 bases:

>100_bases
AATTTTTTCGCCATAGGCGAGCAACTTTTGAGCAAACCGGAACGGTATATGACAGATGCAACGCGCGCATGGTTATACTT
GTTTCTTCTGGTTCTGGCGA

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLRIIKERIHQKYRTLDYPHQQPTLSPRYMGRPSLIPVDCGDCRACYAACPTAALLPTEGVPGSTPTLDMGRCTFCGACR
AACPKQAITFTGEHRMAAFRREDLLIVPGKPVTQPATPPVPGKFAIFRRSLKLRQVSAAGCNACEADCNVLGTLVFDLPR
FGIDFVASPRHADGIVLTGPVSENMRLAAIDTYNATPEPRLVVAVGACAISGGLFRDAPQCNNGIADILPVDLFVPGCPP
NPWTILDGLLMLRE

Sequences:

>Translated_254_residues
MLRIIKERIHQKYRTLDYPHQQPTLSPRYMGRPSLIPVDCGDCRACYAACPTAALLPTEGVPGSTPTLDMGRCTFCGACR
AACPKQAITFTGEHRMAAFRREDLLIVPGKPVTQPATPPVPGKFAIFRRSLKLRQVSAAGCNACEADCNVLGTLVFDLPR
FGIDFVASPRHADGIVLTGPVSENMRLAAIDTYNATPEPRLVVAVGACAISGGLFRDAPQCNNGIADILPVDLFVPGCPP
NPWTILDGLLMLRE
>Mature_254_residues
MLRIIKERIHQKYRTLDYPHQQPTLSPRYMGRPSLIPVDCGDCRACYAACPTAALLPTEGVPGSTPTLDMGRCTFCGACR
AACPKQAITFTGEHRMAAFRREDLLIVPGKPVTQPATPPVPGKFAIFRRSLKLRQVSAAGCNACEADCNVLGTLVFDLPR
FGIDFVASPRHADGIVLTGPVSENMRLAAIDTYNATPEPRLVVAVGACAISGGLFRDAPQCNNGIADILPVDLFVPGCPP
NPWTILDGLLMLRE

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=115, Percent_Identity=35.6521739130435, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=42.2764227642276, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789074, Length=125, Percent_Identity=40.8, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788624, Length=116, Percent_Identity=36.2068965517241, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789075, Length=107, Percent_Identity=33.6448598130841, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17509685, Length=99, Percent_Identity=39.3939393939394, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI18859983, Length=99, Percent_Identity=39.3939393939394, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24642371, Length=99, Percent_Identity=39.3939393939394, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651058, Length=115, Percent_Identity=34.7826086956522, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27387; Mature: 27387

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIIKERIHQKYRTLDYPHQQPTLSPRYMGRPSLIPVDCGDCRACYAACPTAALLPTEG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCEECCCCC
VPGSTPTLDMGRCTFCGACRAACPKQAITFTGEHRMAAFRREDLLIVPGKPVTQPATPPV
CCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHCCCCEEEECCCCCCCCCCCCC
PGKFAIFRRSLKLRQVSAAGCNACEADCNVLGTLVFDLPRFGIDFVASPRHADGIVLTGP
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECC
VSENMRLAAIDTYNATPEPRLVVAVGACAISGGLFRDAPQCNNGIADILPVDLFVPGCPP
CCCCEEEEEEECCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCHHEEEEEEEECCCCC
NPWTILDGLLMLRE
CCHHHHHHHHHHCC
>Mature Secondary Structure
MLRIIKERIHQKYRTLDYPHQQPTLSPRYMGRPSLIPVDCGDCRACYAACPTAALLPTEG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCEECCCCC
VPGSTPTLDMGRCTFCGACRAACPKQAITFTGEHRMAAFRREDLLIVPGKPVTQPATPPV
CCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHCCCCEEEECCCCCCCCCCCCC
PGKFAIFRRSLKLRQVSAAGCNACEADCNVLGTLVFDLPRFGIDFVASPRHADGIVLTGP
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECC
VSENMRLAAIDTYNATPEPRLVVAVGACAISGGLFRDAPQCNNGIADILPVDLFVPGCPP
CCCCEEEEEEECCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCHHEEEEEEEECCCCC
NPWTILDGLLMLRE
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]