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The map label for this gene is zraS [H]

Identifier: 220904042

GI number: 220904042

Start: 899903

End: 901924

Strand: Direct

Name: zraS [H]

Synonym: Ddes_0768

Alternate gene names: 220904042

Gene position: 899903-901924 (Clockwise)

Preceding gene: 220904038

Following gene: 220904043

Centisome position: 31.32

GC content: 62.36

Gene sequence:

>2022_bases
ATGAAAAGCAGAGTCTCAAGACTGTTTTCCGCCAGGGGCCTGCGCGCCCTGCATCTAGGCCTTTCACCCTGGATGGTGGT
CGGCATGGCCATCATTCTCGGCCTTGCCATCACCGCGCTTGCGGTGCGCAGCAATCAGCGCGGCAAGACATATATGATAC
AGAATCTCATAGACAGGGCCGAAGCCCTCATCTGGGCCTTGGAAGCCGGCGCGCGCACAGGGCTGTGCACGCCTCTGGGC
ACGTTGGGTACAACTGACGGCCTTCAATCCTTGCTGACGGAAACCGCCAAGCAGCCCGGCATTGTTTTTATGGCTGTAGC
CAGTGATGAAGGTAAAATGCTTGCCTACAGCGTGCCTGTGCGCAACAGGGACAGAAGCCGGCCAGAGCGCGAAAACGCAT
CTCCGCGAGAAATGACGGGCGTGCAGCCCGGTGAGCGGGCACTGGCCCTCATCATGCCCACCGGGGTGCAGGTAACAGAA
AAGCCCGCCTGGCGCATACGGCAGTGGAACGGAAAAAAAGTTTTTGAGGTTTACCGGGCTTTTGTGCCGCTGGCTGATGT
TCGGCCCATGCCCATGCCCATGCCCATGCGCGGGCATGGGCAACATATGGGCCGCGGGCGCAGTATGGGCGGCGCAGGCA
TGCCGCATGGCCCCATGACCCTGAAAGATATGAGCCCGCCCGGTTCCATGCGGCCCATGCCGGAAGGCAGACACGGGGGC
ATGTCTTCCTCCGGACCAGGCGTCATGCCTGGCGGCGCCGAAGGGCAATGGCAGCAGGAGCAGGTCCGGCAGCCCATGGG
CGTGGTGGTGATCGGGCTTGACCTCAAGCCTTTTGAAGCCGCGCTGGCAGCAGATTTACGCCACAATCTCACCTCGGCGG
CCCTTCTTGCGGCCCTGGGGCTTGCCGGTTTTGTTTCCCTGTTCTGGGCGCACAACTACCGCCGTTCACGACGTATGTTG
CGCGATTCCCGGGCCATGGCTTCGGAGGTCGTTGCCAATCTGCCTCTGGGGCTTCTCACCAGCGACCCTTACGGCAGGGT
CGCCATGGTCAACGACACGGCCCTGAGCATGTTTGAACTGGACAGAAGTACAGCTGTGTACGCGCCCCTGACCGGCCTGC
CAGGTCTGGACTGGAGCGCCCTTGTGGCGGAGCTGGCCAGCGGGCGAAAGGTGCTTGAGCGTGAGTGCGCGCTCACCACA
GGCCGGGCGGGAGCCGTCATCAGCCTAAGTGCGGCATCCATGCACAATCAGGATGGCGTGTTTCTCGGCAACGTGTTCAT
CCTCAGGGACATAACCGAGGTCAAACGCCTGCAGGCCGACGCACAGCGCAACGACCGCCTGTCCGCCTTGGGGCATCTGG
CGGCGGGTGTGGCGCACGAGGTGCGCAATCCCCTGAGCACCATCAAGGGGGTGGCCCTGTATATAGCCAAACGCATGTTG
CCCGGCGGCCGGGAAGAAGAGGCCGCGCAACGCATGATTGACGAAGTGGACAGGCTCGACCGCGTGGTGTCTGAATTGCT
GGAATTTGCCCGGCCCGGAGCCATCAATACGGCTGAAGCCGACCTTGCAGAAGTGATCGGCCGTGCGTTGAGGCTGGCAG
AGGCTGATATGCGCTCACGGCATATTCATGTGGTTCTTGATGTGCCGCCGGGCCTGCCGCCAGTGCGCGTCAACACCGAG
CGGCTTACCCAGGCCCTGCTGAATCTTTTTCTCAACGCTGCGCAGGCTATGGGGGAGGGCGGCGAACTGCGCGTGGGCGC
GCGACTCTGTTCCGGCGGGCAGACCTTCTGCATCACGGTGGCCGATAACGGACCAGGCATTGCCAGAGACATTCAGGCGG
CCATTTTTACGCCCTATTTTACGACCAAGCCTTCAGGCACGGGCCTTGGCCTTGCCATTGTACACCAGATTGTGGAAGGC
CACGGCGGCAATGTCAGCGTGACCTCTGCGCCTGGCCGGGGTACGGAATTTACCCTTGAACTGCCGGTGCGGGGCAGGGA
TGCTGCTCCGGCGGGCGGATAA

Upstream 100 bases:

>100_bases
TTTACTCACATCTGGCGGCGACTGTCCAATTTTTACCCCCGGACTTGACCGGGTGGGTAAAAAATACATAATACAGTCAT
CCTGCAACCAAGGAAGACCC

Downstream 100 bases:

>100_bases
ATGTTCAGACAAAAACAACGGAATACTCAAGCTGCCGAGGAAGATGAAATGACTGCAAAAACGGCCGTAAAGCTGCTGGT
GGTAGATGACGACCGCAATC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MKSRVSRLFSARGLRALHLGLSPWMVVGMAIILGLAITALAVRSNQRGKTYMIQNLIDRAEALIWALEAGARTGLCTPLG
TLGTTDGLQSLLTETAKQPGIVFMAVASDEGKMLAYSVPVRNRDRSRPERENASPREMTGVQPGERALALIMPTGVQVTE
KPAWRIRQWNGKKVFEVYRAFVPLADVRPMPMPMPMRGHGQHMGRGRSMGGAGMPHGPMTLKDMSPPGSMRPMPEGRHGG
MSSSGPGVMPGGAEGQWQQEQVRQPMGVVVIGLDLKPFEAALAADLRHNLTSAALLAALGLAGFVSLFWAHNYRRSRRML
RDSRAMASEVVANLPLGLLTSDPYGRVAMVNDTALSMFELDRSTAVYAPLTGLPGLDWSALVAELASGRKVLERECALTT
GRAGAVISLSAASMHNQDGVFLGNVFILRDITEVKRLQADAQRNDRLSALGHLAAGVAHEVRNPLSTIKGVALYIAKRML
PGGREEEAAQRMIDEVDRLDRVVSELLEFARPGAINTAEADLAEVIGRALRLAEADMRSRHIHVVLDVPPGLPPVRVNTE
RLTQALLNLFLNAAQAMGEGGELRVGARLCSGGQTFCITVADNGPGIARDIQAAIFTPYFTTKPSGTGLGLAIVHQIVEG
HGGNVSVTSAPGRGTEFTLELPVRGRDAAPAGG

Sequences:

>Translated_673_residues
MKSRVSRLFSARGLRALHLGLSPWMVVGMAIILGLAITALAVRSNQRGKTYMIQNLIDRAEALIWALEAGARTGLCTPLG
TLGTTDGLQSLLTETAKQPGIVFMAVASDEGKMLAYSVPVRNRDRSRPERENASPREMTGVQPGERALALIMPTGVQVTE
KPAWRIRQWNGKKVFEVYRAFVPLADVRPMPMPMPMRGHGQHMGRGRSMGGAGMPHGPMTLKDMSPPGSMRPMPEGRHGG
MSSSGPGVMPGGAEGQWQQEQVRQPMGVVVIGLDLKPFEAALAADLRHNLTSAALLAALGLAGFVSLFWAHNYRRSRRML
RDSRAMASEVVANLPLGLLTSDPYGRVAMVNDTALSMFELDRSTAVYAPLTGLPGLDWSALVAELASGRKVLERECALTT
GRAGAVISLSAASMHNQDGVFLGNVFILRDITEVKRLQADAQRNDRLSALGHLAAGVAHEVRNPLSTIKGVALYIAKRML
PGGREEEAAQRMIDEVDRLDRVVSELLEFARPGAINTAEADLAEVIGRALRLAEADMRSRHIHVVLDVPPGLPPVRVNTE
RLTQALLNLFLNAAQAMGEGGELRVGARLCSGGQTFCITVADNGPGIARDIQAAIFTPYFTTKPSGTGLGLAIVHQIVEG
HGGNVSVTSAPGRGTEFTLELPVRGRDAAPAGG
>Mature_673_residues
MKSRVSRLFSARGLRALHLGLSPWMVVGMAIILGLAITALAVRSNQRGKTYMIQNLIDRAEALIWALEAGARTGLCTPLG
TLGTTDGLQSLLTETAKQPGIVFMAVASDEGKMLAYSVPVRNRDRSRPERENASPREMTGVQPGERALALIMPTGVQVTE
KPAWRIRQWNGKKVFEVYRAFVPLADVRPMPMPMPMRGHGQHMGRGRSMGGAGMPHGPMTLKDMSPPGSMRPMPEGRHGG
MSSSGPGVMPGGAEGQWQQEQVRQPMGVVVIGLDLKPFEAALAADLRHNLTSAALLAALGLAGFVSLFWAHNYRRSRRML
RDSRAMASEVVANLPLGLLTSDPYGRVAMVNDTALSMFELDRSTAVYAPLTGLPGLDWSALVAELASGRKVLERECALTT
GRAGAVISLSAASMHNQDGVFLGNVFILRDITEVKRLQADAQRNDRLSALGHLAAGVAHEVRNPLSTIKGVALYIAKRML
PGGREEEAAQRMIDEVDRLDRVVSELLEFARPGAINTAEADLAEVIGRALRLAEADMRSRHIHVVLDVPPGLPPVRVNTE
RLTQALLNLFLNAAQAMGEGGELRVGARLCSGGQTFCITVADNGPGIARDIQAAIFTPYFTTKPSGTGLGLAIVHQIVEG
HGGNVSVTSAPGRGTEFTLELPVRGRDAAPAGG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=50.8474576271186, Blast_Score=225, Evalue=9e-60,
Organism=Escherichia coli, GI1788549, Length=348, Percent_Identity=32.183908045977, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1790300, Length=298, Percent_Identity=30.5369127516779, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=27.710843373494, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790346, Length=250, Percent_Identity=30.4, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=31.5315315315315, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=29.9559471365639, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=282, Percent_Identity=25.886524822695, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790567, Length=419, Percent_Identity=26.9689737470167, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081816, Length=241, Percent_Identity=27.8008298755187, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=28.8990825688073, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=255, Percent_Identity=24.7058823529412, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72040; Mature: 72040

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRVSRLFSARGLRALHLGLSPWMVVGMAIILGLAITALAVRSNQRGKTYMIQNLIDRA
CCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EALIWALEAGARTGLCTPLGTLGTTDGLQSLLTETAKQPGIVFMAVASDEGKMLAYSVPV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCC
RNRDRSRPERENASPREMTGVQPGERALALIMPTGVQVTEKPAWRIRQWNGKKVFEVYRA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHEECCCCHHHHHHHHH
FVPLADVRPMPMPMPMRGHGQHMGRGRSMGGAGMPHGPMTLKDMSPPGSMRPMPEGRHGG
HHCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
MSSSGPGVMPGGAEGQWQQEQVRQPMGVVVIGLDLKPFEAALAADLRHNLTSAALLAALG
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGFVSLFWAHNYRRSRRMLRDSRAMASEVVANLPLGLLTSDPYGRVAMVNDTALSMFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEECCHHHHHHHH
DRSTAVYAPLTGLPGLDWSALVAELASGRKVLERECALTTGRAGAVISLSAASMHNQDGV
CCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCE
FLGNVFILRDITEVKRLQADAQRNDRLSALGHLAAGVAHEVRNPLSTIKGVALYIAKRML
EEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGGREEEAAQRMIDEVDRLDRVVSELLEFARPGAINTAEADLAEVIGRALRLAEADMRSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
HIHVVLDVPPGLPPVRVNTERLTQALLNLFLNAAQAMGEGGELRVGARLCSGGQTFCITV
EEEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEHEECCCCCEEEEEE
ADNGPGIARDIQAAIFTPYFTTKPSGTGLGLAIVHQIVEGHGGNVSVTSAPGRGTEFTLE
ECCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
LPVRGRDAAPAGG
CCCCCCCCCCCCC
>Mature Secondary Structure
MKSRVSRLFSARGLRALHLGLSPWMVVGMAIILGLAITALAVRSNQRGKTYMIQNLIDRA
CCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EALIWALEAGARTGLCTPLGTLGTTDGLQSLLTETAKQPGIVFMAVASDEGKMLAYSVPV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCC
RNRDRSRPERENASPREMTGVQPGERALALIMPTGVQVTEKPAWRIRQWNGKKVFEVYRA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHEECCCCHHHHHHHHH
FVPLADVRPMPMPMPMRGHGQHMGRGRSMGGAGMPHGPMTLKDMSPPGSMRPMPEGRHGG
HHCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
MSSSGPGVMPGGAEGQWQQEQVRQPMGVVVIGLDLKPFEAALAADLRHNLTSAALLAALG
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGFVSLFWAHNYRRSRRMLRDSRAMASEVVANLPLGLLTSDPYGRVAMVNDTALSMFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEECCHHHHHHHH
DRSTAVYAPLTGLPGLDWSALVAELASGRKVLERECALTTGRAGAVISLSAASMHNQDGV
CCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCE
FLGNVFILRDITEVKRLQADAQRNDRLSALGHLAAGVAHEVRNPLSTIKGVALYIAKRML
EEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGGREEEAAQRMIDEVDRLDRVVSELLEFARPGAINTAEADLAEVIGRALRLAEADMRSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
HIHVVLDVPPGLPPVRVNTERLTQALLNLFLNAAQAMGEGGELRVGARLCSGGQTFCITV
EEEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEHEECCCCCEEEEEE
ADNGPGIARDIQAAIFTPYFTTKPSGTGLGLAIVHQIVEGHGGNVSVTSAPGRGTEFTLE
ECCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
LPVRGRDAAPAGG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]