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The map label for this gene is rlmH

Identifier: 220904021

GI number: 220904021

Start: 882028

End: 882501

Strand: Reverse

Name: rlmH

Synonym: Ddes_0747

Alternate gene names: 220904021

Gene position: 882501-882028 (Counterclockwise)

Preceding gene: 220904023

Following gene: 220904018

Centisome position: 30.71

GC content: 63.29

Gene sequence:

>474_bases
ATGGCAGGCAAACCTTTACGGTGCATCAGTGTGGGCAAGTTGAAAACACCTTTCTGGAAGGACGCCGCAGCCCACTACCT
TACGCGCATCAAACGCTGGCGGCATCTGGAATATACTGAAGTGCGCGACGGCGATGCCGCCCTGCCTCCTGACCAGCGTA
ACGCCCTTGAAGGACGCCGCATCGTTGAAGCTCTTGCGCCGCAGGACGTGGCCCTTGTGCTGGACGAACACGGCCAGAAG
CTCTCGTCGCCCCAACTGGCCGCCCTGCTGCGCCGCCTGGATCAGGAGGCACGCGGAAGGGCCTGCTTTGTCGTGGGCGG
AGCCTGGGGCCTGGACGACAGCGTGCGGCAGCGGGCCTTCAAGGTCCTGAGCCTCTCGGACATGACCCTGCCCCACGAAC
TGGCCCGCGTGGTCCTGCTTGAGCAGATATACCGCGCCGAATGCATCCTGCGCAAAGTGCCCTACCACCATTGA

Upstream 100 bases:

>100_bases
GCCGAGACCATAAAATGTTTCGGTCGGAAAAATCAGTGCATGACCGCCGCGCAGGCAGGCAACGGCCTCTTGCAGATCGC
GGCAGACTATTTGGAGAGAC

Downstream 100 bases:

>100_bases
CGTTTCCGGATACCGCAAGCTGCTTTTTTACAATACCTCGTAAATCCTGGCGAAAACATTATCGTACACCAGCTTGAAGT
ATGGTGAAAAACGCGGGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAGKPLRCISVGKLKTPFWKDAAAHYLTRIKRWRHLEYTEVRDGDAALPPDQRNALEGRRIVEALAPQDVALVLDEHGQK
LSSPQLAALLRRLDQEARGRACFVVGGAWGLDDSVRQRAFKVLSLSDMTLPHELARVVLLEQIYRAECILRKVPYHH

Sequences:

>Translated_157_residues
MAGKPLRCISVGKLKTPFWKDAAAHYLTRIKRWRHLEYTEVRDGDAALPPDQRNALEGRRIVEALAPQDVALVLDEHGQK
LSSPQLAALLRRLDQEARGRACFVVGGAWGLDDSVRQRAFKVLSLSDMTLPHELARVVLLEQIYRAECILRKVPYHH
>Mature_156_residues
AGKPLRCISVGKLKTPFWKDAAAHYLTRIKRWRHLEYTEVRDGDAALPPDQRNALEGRRIVEALAPQDVALVLDEHGQKL
SSPQLAALLRRLDQEARGRACFVVGGAWGLDDSVRQRAFKVLSLSDMTLPHELARVVLLEQIYRAECILRKVPYHH

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=154, Percent_Identity=33.7662337662338, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_DESDA (B8IYS8)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479333.1
- ProteinModelPortal:   B8IYS8
- GeneID:   7284421
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0747
- HOGENOM:   HBG622807
- ProtClustDB:   CLSK705132
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17812; Mature: 17681

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKPLRCISVGKLKTPFWKDAAAHYLTRIKRWRHLEYTEVRDGDAALPPDQRNALEGRR
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
IVEALAPQDVALVLDEHGQKLSSPQLAALLRRLDQEARGRACFVVGGAWGLDDSVRQRAF
HHHHHCCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
KVLSLSDMTLPHELARVVLLEQIYRAECILRKVPYHH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGKPLRCISVGKLKTPFWKDAAAHYLTRIKRWRHLEYTEVRDGDAALPPDQRNALEGRR
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
IVEALAPQDVALVLDEHGQKLSSPQLAALLRRLDQEARGRACFVVGGAWGLDDSVRQRAF
HHHHHCCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
KVLSLSDMTLPHELARVVLLEQIYRAECILRKVPYHH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA