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The map label for this gene is ruvC [H]

Identifier: 220904014

GI number: 220904014

Start: 873100

End: 873621

Strand: Reverse

Name: ruvC [H]

Synonym: Ddes_0740

Alternate gene names: 220904014

Gene position: 873621-873100 (Counterclockwise)

Preceding gene: 220904015

Following gene: 220904012

Centisome position: 30.4

GC content: 60.34

Gene sequence:

>522_bases
ATGCCGACGCTCAACGCCAAAGGGCCTGTCACGGTTATGGGCATAGACCCCGGCTCGCAACGCACGGGCTGGGGCGTGGT
GCGCGAAGTTTCAGGCGTGTTGCAGCTGGTGGACTGCGGCGTGGTGCGTACTGCTTCGGCGGGCAAGGCATTTTCAGACC
GTCTGGCATGCATCTACCATGAGCTTTCCACGGTGCTAAGCCGCCTCAAGCCTGAAGAAGCTGCCATTGAACAGGTATTT
ACAGCCCAGAATGCGGCCAGCGCGCTCAAGCTCGGTCAGGCGCGGGGTGTGGCTGTGGCGGCCTGCGCGGCACATGGCCT
CTCTATCAGCGATTACGAACCCACCCTGGTGAAAAAATCTCTGGTGGGTACGGGCCGTGCCGAAAAAGAGCAGGTAGCCT
TTATGGTGCAGCGTCTGCTTAATGTCAAAAACGCCAGCTGGTCGCTGGACACTTCAGACGCTCTGGCCGTGGCTGTGTGC
CACCTTACCATGCGGCGCTTCGCGGCCTTGACAGGCAAATAG

Upstream 100 bases:

>100_bases
GGGCCGGAAGCCGTGGGCAATGCCACCACTTCGGCCGTGGTCATATCCTGCGTGCTGATTCTGGCCGCAGACTACGTTAT
CACCTCTTTCCTGCTCTGAT

Downstream 100 bases:

>100_bases
CCCATATGGCTGAACACCGCCTGTATTTGGGCCGGGTCCGCCAAGATCTTTTTTGTCTCCGCCACAAAAACAGTTACGAA
TACACAGCCCGACCTTCGTG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MPTLNAKGPVTVMGIDPGSQRTGWGVVREVSGVLQLVDCGVVRTASAGKAFSDRLACIYHELSTVLSRLKPEEAAIEQVF
TAQNAASALKLGQARGVAVAACAAHGLSISDYEPTLVKKSLVGTGRAEKEQVAFMVQRLLNVKNASWSLDTSDALAVAVC
HLTMRRFAALTGK

Sequences:

>Translated_173_residues
MPTLNAKGPVTVMGIDPGSQRTGWGVVREVSGVLQLVDCGVVRTASAGKAFSDRLACIYHELSTVLSRLKPEEAAIEQVF
TAQNAASALKLGQARGVAVAACAAHGLSISDYEPTLVKKSLVGTGRAEKEQVAFMVQRLLNVKNASWSLDTSDALAVAVC
HLTMRRFAALTGK
>Mature_172_residues
PTLNAKGPVTVMGIDPGSQRTGWGVVREVSGVLQLVDCGVVRTASAGKAFSDRLACIYHELSTVLSRLKPEEAAIEQVFT
AQNAASALKLGQARGVAVAACAAHGLSISDYEPTLVKKSLVGTGRAEKEQVAFMVQRLLNVKNASWSLDTSDALAVAVCH
LTMRRFAALTGK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=153, Percent_Identity=41.8300653594771, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18224; Mature: 18093

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLNAKGPVTVMGIDPGSQRTGWGVVREVSGVLQLVDCGVVRTASAGKAFSDRLACIYH
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHH
ELSTVLSRLKPEEAAIEQVFTAQNAASALKLGQARGVAVAACAAHGLSISDYEPTLVKKS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
LVGTGRAEKEQVAFMVQRLLNVKNASWSLDTSDALAVAVCHLTMRRFAALTGK
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTLNAKGPVTVMGIDPGSQRTGWGVVREVSGVLQLVDCGVVRTASAGKAFSDRLACIYH
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHH
ELSTVLSRLKPEEAAIEQVFTAQNAASALKLGQARGVAVAACAAHGLSISDYEPTLVKKS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
LVGTGRAEKEQVAFMVQRLLNVKNASWSLDTSDALAVAVCHLTMRRFAALTGK
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA