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The map label for this gene is truA [H]

Identifier: 220903970

GI number: 220903970

Start: 826866

End: 827645

Strand: Reverse

Name: truA [H]

Synonym: Ddes_0696

Alternate gene names: 220903970

Gene position: 827645-826866 (Counterclockwise)

Preceding gene: 220903971

Following gene: 220903962

Centisome position: 28.8

GC content: 63.33

Gene sequence:

>780_bases
ATGCGCCTGAAACTTCTTATTTCCTACGTGGGCACTGGCTACAGCGGCTGGCAGATTCAGGAAAAACCCAAACCGCCGCC
TACGGTGCAGGGCGCTCTTGAAGCCGCACTGCGTACCATTGCCGGTAAAGCCGTGCGCGTTCACGGTTCGGGCCGCACTG
ATTCCGGTGTACATGCCCACGGCCAGGTGGCCCATTGCGACATCCCCGATACCCGGGCCGGGCTTGACTGGCGGCACAGC
CTCAATGCCGTGCTGCCGCGCGATATCCGCGTACTGGACGCCTCCCCCGCTGCCCCGGACTTTCATGCCCGCAAAGACGC
CCTGCGCAAAACCTATGCCTACCAGTTCTGGCAGGACCGCCGCTTTCTGCCGCCGCACGTGCGCCCGTATGTATGGAACT
GCGGCATGCTGGACCTGGACGCCATGCGCGATGCCCTGCCCTTTCTTCTGGGCAGGCACGACTTCGCCGGCCTGCGTAAT
GCCGGTACGGATGTGGAGAGTACCGAACGTACCATTATGGACGCCCGCCTGGACGCACTGGCCCCCTGCGAATACTACCC
CCCCCATGCCCCCATGCTGCGCCTTACCATTACGGCCGATGGCTTTCTGAAACAGATGGTACGCAATGTGGCCGGTCTGC
TTGCCGCGTGCGGTCAAGGCAAAATACAGCCCGCGCAGATACCTGCGCTGCTTGCAGCCCGCGACCGCCAGGCAGTGCCT
TCGGTAACGGCCCCACCCGAGGGGCTGGCGCTGGTGAGTGTGAAATATCCAGAGCCATAG

Upstream 100 bases:

>100_bases
GCATGTCCCCCAAGCAGTCCGGCGACATCCTCACCTACGTCAACCCGGCAAAAACCGCCAAACTGACGGAAATCATCACC
CGCATGAAGCTGCCAGAATA

Downstream 100 bases:

>100_bases
CTTTATATGCTGCCCGCAGCACACATTGGCAACAAACCATTCTTTCGCTCACTAACGCAAAAGTCCCCACCGCAAGATGG
GGACTTTTATATATTTCACG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRLKLLISYVGTGYSGWQIQEKPKPPPTVQGALEAALRTIAGKAVRVHGSGRTDSGVHAHGQVAHCDIPDTRAGLDWRHS
LNAVLPRDIRVLDASPAAPDFHARKDALRKTYAYQFWQDRRFLPPHVRPYVWNCGMLDLDAMRDALPFLLGRHDFAGLRN
AGTDVESTERTIMDARLDALAPCEYYPPHAPMLRLTITADGFLKQMVRNVAGLLAACGQGKIQPAQIPALLAARDRQAVP
SVTAPPEGLALVSVKYPEP

Sequences:

>Translated_259_residues
MRLKLLISYVGTGYSGWQIQEKPKPPPTVQGALEAALRTIAGKAVRVHGSGRTDSGVHAHGQVAHCDIPDTRAGLDWRHS
LNAVLPRDIRVLDASPAAPDFHARKDALRKTYAYQFWQDRRFLPPHVRPYVWNCGMLDLDAMRDALPFLLGRHDFAGLRN
AGTDVESTERTIMDARLDALAPCEYYPPHAPMLRLTITADGFLKQMVRNVAGLLAACGQGKIQPAQIPALLAARDRQAVP
SVTAPPEGLALVSVKYPEP
>Mature_259_residues
MRLKLLISYVGTGYSGWQIQEKPKPPPTVQGALEAALRTIAGKAVRVHGSGRTDSGVHAHGQVAHCDIPDTRAGLDWRHS
LNAVLPRDIRVLDASPAAPDFHARKDALRKTYAYQFWQDRRFLPPHVRPYVWNCGMLDLDAMRDALPFLLGRHDFAGLRN
AGTDVESTERTIMDARLDALAPCEYYPPHAPMLRLTITADGFLKQMVRNVAGLLAACGQGKIQPAQIPALLAARDRQAVP
SVTAPPEGLALVSVKYPEP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=33.695652173913, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI199559785, Length=281, Percent_Identity=29.8932384341637, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788657, Length=263, Percent_Identity=34.6007604562738, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71985046, Length=279, Percent_Identity=26.1648745519713, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922728, Length=266, Percent_Identity=30.0751879699248, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28420; Mature: 28420

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKLLISYVGTGYSGWQIQEKPKPPPTVQGALEAALRTIAGKAVRVHGSGRTDSGVHAH
CCCEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
GQVAHCDIPDTRAGLDWRHSLNAVLPRDIRVLDASPAAPDFHARKDALRKTYAYQFWQDR
CCEEECCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RFLPPHVRPYVWNCGMLDLDAMRDALPFLLGRHDFAGLRNAGTDVESTERTIMDARLDAL
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
APCEYYPPHAPMLRLTITADGFLKQMVRNVAGLLAACGQGKIQPAQIPALLAARDRQAVP
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
SVTAPPEGLALVSVKYPEP
CCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRLKLLISYVGTGYSGWQIQEKPKPPPTVQGALEAALRTIAGKAVRVHGSGRTDSGVHAH
CCCEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
GQVAHCDIPDTRAGLDWRHSLNAVLPRDIRVLDASPAAPDFHARKDALRKTYAYQFWQDR
CCEEECCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RFLPPHVRPYVWNCGMLDLDAMRDALPFLLGRHDFAGLRNAGTDVESTERTIMDARLDAL
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
APCEYYPPHAPMLRLTITADGFLKQMVRNVAGLLAACGQGKIQPAQIPALLAARDRQAVP
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
SVTAPPEGLALVSVKYPEP
CCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA