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The map label for this gene is lysS [H]

Identifier: 220903895

GI number: 220903895

Start: 749116

End: 750627

Strand: Reverse

Name: lysS [H]

Synonym: Ddes_0620

Alternate gene names: 220903895

Gene position: 750627-749116 (Counterclockwise)

Preceding gene: 220903899

Following gene: 220903888

Centisome position: 26.12

GC content: 57.54

Gene sequence:

>1512_bases
ATGCCCATGTTGGAAAGCTTCGCCGCCCGTGAAGGGTTAAACGAGGTCGTAAAAAACCGCGTGGTCAAAGCCTGTGACCT
CCTGGACGCGGGCGTACCGCTCTTTCCTAACGATTTCCGCAAGGAACATTCCATTGCCTGGGTGCTGGACAAATTCGGCG
ACCTGGAGGGCGATGCCCTGGAAAAACAGGAAGATGTCGTCGCCATTGCCGGGCGCATCGTTTCGCTCCGTTCTTTCGGC
AAGGTGGTCTTTTTCCATATCATGGACCAGACGGGCCGCGTGCAGTGCTACGCCGCGCGCGAACATCTGGACGACGCCAC
GCACAGCCTGGTGAAAAAGCTTGATATCGGCGACATTGTCGGCGTTTCCGGGCATCTTTTCCGCACCAAGACGGGCGAGT
TGACCATCGCCTGCCGCACCATAAAGCTCATCACGCGTTCCATGCGCCCCCTGCCCGAAAAGTATCACGGGCTCAAGGAT
ATGGAAACGCGCTACCGCCAGCGCTATGTGGACCTTATCGTCACACCCCGCGCCCGCGAAATATTTTTCAAACGCAGCCA
GATCGTGCGCGAATTCCGTCATTTTATGGAAGACCACGGCTTCATGGAAGTAGAAACGCCCATGATGCAGCCCCTGGCCG
GGGGCGCGGCGGCAAAGCCGTTCAAAACCCATCACAATGCTCTGGACATGGGCCTGTACATGCGCATTGCGCCCGAGCTG
TACCTCAAGCGCCTGCTTGTGGGCGGTTTTGAAAAAGTGTTTGAGCTGAACCGCAACTTCCGCAATGAAGGGATCGACAC
GCGGCACAATCCCGAATTCACCATGTGCGAATTCTACTGGGCCTACGCCACGTTTGAAGATCTCATGGACCTTACGGAAA
AACTCTTTGCGCATCTGGCGCAGACGGTTTGCGGCACAACCGTGGTTCCCTACCAGGGCGAAATGATCGACCTCACGCCC
GGCAAGTGGACACGCATGAGTTTTTACGACTCGCTCACCGTCATTGGCGGCCACCAGCCGGAATTTTACAATGACTACAG
CAGGGTGCAAAACTACATCCGCCAGCGCGGTGAAAAGGCCGCTGACAGCGAAAGCCTGCAAAAGCTGCACGCCAAGCTTT
TTGACCTGGACGTGGAAGGCAAGCTCGTCCAGCCGCACTTCATCTACCATTACCCCACGGAAATTTCGCCCCTGTCGCGT
CGTAACGACAACGACCCCGCGCTGACCGACCGCTTTGAGCTTTTCATTACCGGCCGCGAGATATCCAACGCCTTTTCCGA
ACTTAACGACCCCGTGGACCAGCGCCTGCGTTTTGAAGACCAGGTGCGCGAAAAAGAAGCCGGAGACGATGAGGCGCACA
GTATGGACGAAGACTACCTGCGCGCCCTTGAATACGGCATGCCACCTGCTGCCGGCCAGGGTGTGGGCATTGACCGGCTG
GTCATGCTGCTGACGGACTCCGCCTCCATACGTGAGGTCATCCTCTTCCCCCTGCTGCGGCCGGAATCCTAG

Upstream 100 bases:

>100_bases
ACCGGGCATCTCAGGAGTTTTTTCAGTGGAAAATCGGGAAAAGAAGAATAAAAAGCCTGCCGTCAAGCTGGGCACCAAGT
CTAAGTACGCGGGCTATTTC

Downstream 100 bases:

>100_bases
AATATTTTACTTTTGAAACAGTACTGAAAACGGCCCCCAAAACGGCCGGTGCCTGCTGACAAAAAGGCCCGTTCCCCACA
GAAAGCGTACCTGCCGGTAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MPMLESFAAREGLNEVVKNRVVKACDLLDAGVPLFPNDFRKEHSIAWVLDKFGDLEGDALEKQEDVVAIAGRIVSLRSFG
KVVFFHIMDQTGRVQCYAAREHLDDATHSLVKKLDIGDIVGVSGHLFRTKTGELTIACRTIKLITRSMRPLPEKYHGLKD
METRYRQRYVDLIVTPRAREIFFKRSQIVREFRHFMEDHGFMEVETPMMQPLAGGAAAKPFKTHHNALDMGLYMRIAPEL
YLKRLLVGGFEKVFELNRNFRNEGIDTRHNPEFTMCEFYWAYATFEDLMDLTEKLFAHLAQTVCGTTVVPYQGEMIDLTP
GKWTRMSFYDSLTVIGGHQPEFYNDYSRVQNYIRQRGEKAADSESLQKLHAKLFDLDVEGKLVQPHFIYHYPTEISPLSR
RNDNDPALTDRFELFITGREISNAFSELNDPVDQRLRFEDQVREKEAGDDEAHSMDEDYLRALEYGMPPAAGQGVGIDRL
VMLLTDSASIREVILFPLLRPES

Sequences:

>Translated_503_residues
MPMLESFAAREGLNEVVKNRVVKACDLLDAGVPLFPNDFRKEHSIAWVLDKFGDLEGDALEKQEDVVAIAGRIVSLRSFG
KVVFFHIMDQTGRVQCYAAREHLDDATHSLVKKLDIGDIVGVSGHLFRTKTGELTIACRTIKLITRSMRPLPEKYHGLKD
METRYRQRYVDLIVTPRAREIFFKRSQIVREFRHFMEDHGFMEVETPMMQPLAGGAAAKPFKTHHNALDMGLYMRIAPEL
YLKRLLVGGFEKVFELNRNFRNEGIDTRHNPEFTMCEFYWAYATFEDLMDLTEKLFAHLAQTVCGTTVVPYQGEMIDLTP
GKWTRMSFYDSLTVIGGHQPEFYNDYSRVQNYIRQRGEKAADSESLQKLHAKLFDLDVEGKLVQPHFIYHYPTEISPLSR
RNDNDPALTDRFELFITGREISNAFSELNDPVDQRLRFEDQVREKEAGDDEAHSMDEDYLRALEYGMPPAAGQGVGIDRL
VMLLTDSASIREVILFPLLRPES
>Mature_502_residues
PMLESFAAREGLNEVVKNRVVKACDLLDAGVPLFPNDFRKEHSIAWVLDKFGDLEGDALEKQEDVVAIAGRIVSLRSFGK
VVFFHIMDQTGRVQCYAAREHLDDATHSLVKKLDIGDIVGVSGHLFRTKTGELTIACRTIKLITRSMRPLPEKYHGLKDM
ETRYRQRYVDLIVTPRAREIFFKRSQIVREFRHFMEDHGFMEVETPMMQPLAGGAAAKPFKTHHNALDMGLYMRIAPELY
LKRLLVGGFEKVFELNRNFRNEGIDTRHNPEFTMCEFYWAYATFEDLMDLTEKLFAHLAQTVCGTTVVPYQGEMIDLTPG
KWTRMSFYDSLTVIGGHQPEFYNDYSRVQNYIRQRGEKAADSESLQKLHAKLFDLDVEGKLVQPHFIYHYPTEISPLSRR
NDNDPALTDRFELFITGREISNAFSELNDPVDQRLRFEDQVREKEAGDDEAHSMDEDYLRALEYGMPPAAGQGVGIDRLV
MLLTDSASIREVILFPLLRPES

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=485, Percent_Identity=42.4742268041237, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI194272210, Length=485, Percent_Identity=42.4742268041237, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI45439306, Length=486, Percent_Identity=24.0740740740741, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI40789249, Length=273, Percent_Identity=23.4432234432234, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790571, Length=514, Percent_Identity=50.3891050583658, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789256, Length=497, Percent_Identity=51.5090543259557, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=31.4024390243902, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788173, Length=291, Percent_Identity=24.7422680412371, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=490, Percent_Identity=43.0612244897959, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535927, Length=490, Percent_Identity=43.0612244897959, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71994340, Length=490, Percent_Identity=43.0612244897959, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17551876, Length=351, Percent_Identity=24.2165242165242, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=224, Percent_Identity=26.3392857142857, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=499, Percent_Identity=42.4849699398798, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=31.3025210084034, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=366, Percent_Identity=26.775956284153, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=186, Percent_Identity=26.3440860215054, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=43.3264887063655, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=487, Percent_Identity=43.3264887063655, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57820; Mature: 57689

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMLESFAAREGLNEVVKNRVVKACDLLDAGVPLFPNDFRKEHSIAWVLDKFGDLEGDAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHCCCCCCHH
EKQEDVVAIAGRIVSLRSFGKVVFFHIMDQTGRVQCYAAREHLDDATHSLVKKLDIGDIV
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEE
GVSGHLFRTKTGELTIACRTIKLITRSMRPLPEKYHGLKDMETRYRQRYVDLIVTPRARE
CCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHEECCCHHH
IFFKRSQIVREFRHFMEDHGFMEVETPMMQPLAGGAAAKPFKTHHNALDMGLYMRIAPEL
HHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHH
YLKRLLVGGFEKVFELNRNFRNEGIDTRHNPEFTMCEFYWAYATFEDLMDLTEKLFAHLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVCGTTVVPYQGEMIDLTPGKWTRMSFYDSLTVIGGHQPEFYNDYSRVQNYIRQRGEKA
HHHHCCEECCCCCCEEECCCCCEEEEEHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
ADSESLQKLHAKLFDLDVEGKLVQPHFIYHYPTEISPLSRRNDNDPALTDRFELFITGRE
CCHHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCCCHHCCCCCCCCCCCCEEEEEECHH
ISNAFSELNDPVDQRLRFEDQVREKEAGDDEAHSMDEDYLRALEYGMPPAAGQGVGIDRL
HHHHHHHHCCCHHHHHCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
VMLLTDSASIREVILFPLLRPES
HHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PMLESFAAREGLNEVVKNRVVKACDLLDAGVPLFPNDFRKEHSIAWVLDKFGDLEGDAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHCCCCCCHH
EKQEDVVAIAGRIVSLRSFGKVVFFHIMDQTGRVQCYAAREHLDDATHSLVKKLDIGDIV
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEE
GVSGHLFRTKTGELTIACRTIKLITRSMRPLPEKYHGLKDMETRYRQRYVDLIVTPRARE
CCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHEECCCHHH
IFFKRSQIVREFRHFMEDHGFMEVETPMMQPLAGGAAAKPFKTHHNALDMGLYMRIAPEL
HHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHH
YLKRLLVGGFEKVFELNRNFRNEGIDTRHNPEFTMCEFYWAYATFEDLMDLTEKLFAHLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVCGTTVVPYQGEMIDLTPGKWTRMSFYDSLTVIGGHQPEFYNDYSRVQNYIRQRGEKA
HHHHCCEECCCCCCEEECCCCCEEEEEHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
ADSESLQKLHAKLFDLDVEGKLVQPHFIYHYPTEISPLSRRNDNDPALTDRFELFITGRE
CCHHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCCCHHCCCCCCCCCCCCEEEEEECHH
ISNAFSELNDPVDQRLRFEDQVREKEAGDDEAHSMDEDYLRALEYGMPPAAGQGVGIDRL
HHHHHHHHCCCHHHHHCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
VMLLTDSASIREVILFPLLRPES
HHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA