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The map label for this gene is hybA [H]

Identifier: 220903821

GI number: 220903821

Start: 655422

End: 656861

Strand: Direct

Name: hybA [H]

Synonym: Ddes_0544

Alternate gene names: 220903821

Gene position: 655422-656861 (Clockwise)

Preceding gene: 220903819

Following gene: 220903829

Centisome position: 22.81

GC content: 62.15

Gene sequence:

>1440_bases
ATGAAACGTTCACGACGTATGTTTCTTATGGGGGCCGGGGCGGCGGGCCTGTCGACTATGGCGGGGCACGGCAGCGGCGA
TGCGCTGGCTGCCGCCGGCGGCACTCAGTACGCCACATTGCTGGAACTTGAAAAATGCATCGGCTGCGGCGCATGTGTGC
AGGGCTGCCGCGAGCGCAACGGCACGCGGTATCCCGTGGTGAGCAGACCCATGCCCGAACTGTTTCCTCCGGGAACCAAG
ACCGAAGACTGGTCACAACGGCAGGGTGTGGACGACAGGCTTACCCCCTACAACTGGCTGTACATTGAAACCGTGACCGT
GCAAAAAAACGGCGCAAACCTTGACCTGCACATACCGCGCCGGTGTATGCACTGCACCAATCCGCCCTGTGCCAATCTCT
GCCCCTGGGGAGCGTGCAGCCGTGATCCGCAGACCGGCACTGTGAATATCAGCCCGTCAACCTGCCTTGGCGGGGCCAAG
TGCCGCACGGTTTGCCCGTGGCATGTGCCACAGCGTCAGTCCGGGGTAGGGCCATACCTGCATCTTATGCCGCGCTTTGC
AGGCAACGGCGTCATGTACAAATGCGACCGCTGCGCGGATAGTTACGCAGGGGGGCAACTGCCCGCATGTATTGAAGTCT
GCCCCGAGCAGGTGCAGACCATCGGTCCCCGCGCAGAACTGCTTGCCCATGCGCAGGCGCTTGCGGCCGAGCGCGGCCAC
TATCTTTACGGTGTGGCGGAAAACGGTGGCACCAACACATTTTACCTGTCGCCCGTACCGTTTGAAGATCTGGCTGTCGC
AAGAGAGGCGGGACCGGGAAGGCCCACTCTGGCGGATGTGCCGGACAGCATGGCACAGGCCGCAAACCTGGGCCGCATGC
TTGTGGCGGCCCCGTTGGCGGGTATAGCGGCCGGCATGGCGCGAAGCGTCAAAAGCGGCAGTGCCGCGCTGGATGAAAAG
CAGGCGGCGGCAAAGCCCGCGTCGCTGGCGCTTCCCGGCTGGATAAAACGTGTCTGGGTTGCTGTGGCCCTTATCCTGGG
CTTTACCGGCATGATGCAGATGCCCATAGCCGCGCGCTATGGTTTAACCCGGTTGCCCGGCATGGGCTGGACCGGAAATT
TTTATACTACCCTGAATATCCACTATGTGGCGGGTGCGGTGCTGATAGCGCTGTGCTGCCTGCTGATAGCGCTCAGACTG
AAAGCGGGCGGCGGTTTTCCCCGGTTTGTGTTCTGGGGTGCGGTGCGCGCCTCACTGGTTGTGGGGCTTGTATTGACGGG
CATTTTTCGCGTGTTGAAAAATCTGCCTGCATTTTCTTTTGCGCCGGAACTGGTCATGGGTATGGATCTTGCGCATCTGG
GACTTGCCGCCGTTCTCGGCGCGCTGTCTCTGGCGCTGTGGGTCAGCGGCCGCAAGGCCTGGACAGGCCCGGCCCGGTAA

Upstream 100 bases:

>100_bases
CCAGCGCCGCCTGTCTTTTACGGGTGCTTTGCTTTTGTGGAAAAAAGCGGGCATAAAAGAGCAGGAATGTATACTGATAA
TACAAGGTGCAGGCTAGGTT

Downstream 100 bases:

>100_bases
CGCATCCTCGGCCCGAGGAGTGCGCTTTTTCGCGGAATGGGCCGTTTACCGTCCTGTGCGCAATGCCGGGCGGCAAACGG
CCCATTTTTGTTTTTGCTGG

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKRSRRMFLMGAGAAGLSTMAGHGSGDALAAAGGTQYATLLELEKCIGCGACVQGCRERNGTRYPVVSRPMPELFPPGTK
TEDWSQRQGVDDRLTPYNWLYIETVTVQKNGANLDLHIPRRCMHCTNPPCANLCPWGACSRDPQTGTVNISPSTCLGGAK
CRTVCPWHVPQRQSGVGPYLHLMPRFAGNGVMYKCDRCADSYAGGQLPACIEVCPEQVQTIGPRAELLAHAQALAAERGH
YLYGVAENGGTNTFYLSPVPFEDLAVAREAGPGRPTLADVPDSMAQAANLGRMLVAAPLAGIAAGMARSVKSGSAALDEK
QAAAKPASLALPGWIKRVWVAVALILGFTGMMQMPIAARYGLTRLPGMGWTGNFYTTLNIHYVAGAVLIALCCLLIALRL
KAGGGFPRFVFWGAVRASLVVGLVLTGIFRVLKNLPAFSFAPELVMGMDLAHLGLAAVLGALSLALWVSGRKAWTGPAR

Sequences:

>Translated_479_residues
MKRSRRMFLMGAGAAGLSTMAGHGSGDALAAAGGTQYATLLELEKCIGCGACVQGCRERNGTRYPVVSRPMPELFPPGTK
TEDWSQRQGVDDRLTPYNWLYIETVTVQKNGANLDLHIPRRCMHCTNPPCANLCPWGACSRDPQTGTVNISPSTCLGGAK
CRTVCPWHVPQRQSGVGPYLHLMPRFAGNGVMYKCDRCADSYAGGQLPACIEVCPEQVQTIGPRAELLAHAQALAAERGH
YLYGVAENGGTNTFYLSPVPFEDLAVAREAGPGRPTLADVPDSMAQAANLGRMLVAAPLAGIAAGMARSVKSGSAALDEK
QAAAKPASLALPGWIKRVWVAVALILGFTGMMQMPIAARYGLTRLPGMGWTGNFYTTLNIHYVAGAVLIALCCLLIALRL
KAGGGFPRFVFWGAVRASLVVGLVLTGIFRVLKNLPAFSFAPELVMGMDLAHLGLAAVLGALSLALWVSGRKAWTGPAR
>Mature_479_residues
MKRSRRMFLMGAGAAGLSTMAGHGSGDALAAAGGTQYATLLELEKCIGCGACVQGCRERNGTRYPVVSRPMPELFPPGTK
TEDWSQRQGVDDRLTPYNWLYIETVTVQKNGANLDLHIPRRCMHCTNPPCANLCPWGACSRDPQTGTVNISPSTCLGGAK
CRTVCPWHVPQRQSGVGPYLHLMPRFAGNGVMYKCDRCADSYAGGQLPACIEVCPEQVQTIGPRAELLAHAQALAAERGH
YLYGVAENGGTNTFYLSPVPFEDLAVAREAGPGRPTLADVPDSMAQAANLGRMLVAAPLAGIAAGMARSVKSGSAALDEK
QAAAKPASLALPGWIKRVWVAVALILGFTGMMQMPIAARYGLTRLPGMGWTGNFYTTLNIHYVAGAVLIALCCLLIALRL
KAGGGFPRFVFWGAVRASLVVGLVLTGIFRVLKNLPAFSFAPELVMGMDLAHLGLAAVLGALSLALWVSGRKAWTGPAR

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=354, Percent_Identity=29.9435028248588, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI2367345, Length=271, Percent_Identity=27.3062730627306, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787749, Length=230, Percent_Identity=26.9565217391304, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI226510944, Length=253, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790326, Length=228, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787122, Length=202, Percent_Identity=28.7128712871287, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787872, Length=202, Percent_Identity=28.7128712871287, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50782; Mature: 50782

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSRRMFLMGAGAAGLSTMAGHGSGDALAAAGGTQYATLLELEKCIGCGACVQGCRERN
CCCCCEEEEEECCCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCC
GTRYPVVSRPMPELFPPGTKTEDWSQRQGVDDRLTPYNWLYIETVTVQKNGANLDLHIPR
CCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCH
RCMHCTNPPCANLCPWGACSRDPQTGTVNISPSTCLGGAKCRTVCPWHVPQRQSGVGPYL
HHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHH
HLMPRFAGNGVMYKCDRCADSYAGGQLPACIEVCPEQVQTIGPRAELLAHAQALAAERGH
HHHHHHCCCCCEEEEHHHHHHCCCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCCC
YLYGVAENGGTNTFYLSPVPFEDLAVAREAGPGRPTLADVPDSMAQAANLGRMLVAAPLA
EEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCHHHHHHHHH
GIAAGMARSVKSGSAALDEKQAAAKPASLALPGWIKRVWVAVALILGFTGMMQMPIAARY
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GLTRLPGMGWTGNFYTTLNIHYVAGAVLIALCCLLIALRLKAGGGFPRFVFWGAVRASLV
CCHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGLVLTGIFRVLKNLPAFSFAPELVMGMDLAHLGLAAVLGALSLALWVSGRKAWTGPAR
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRSRRMFLMGAGAAGLSTMAGHGSGDALAAAGGTQYATLLELEKCIGCGACVQGCRERN
CCCCCEEEEEECCCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCC
GTRYPVVSRPMPELFPPGTKTEDWSQRQGVDDRLTPYNWLYIETVTVQKNGANLDLHIPR
CCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCH
RCMHCTNPPCANLCPWGACSRDPQTGTVNISPSTCLGGAKCRTVCPWHVPQRQSGVGPYL
HHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHH
HLMPRFAGNGVMYKCDRCADSYAGGQLPACIEVCPEQVQTIGPRAELLAHAQALAAERGH
HHHHHHCCCCCEEEEHHHHHHCCCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCCC
YLYGVAENGGTNTFYLSPVPFEDLAVAREAGPGRPTLADVPDSMAQAANLGRMLVAAPLA
EEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCHHHHHHHHH
GIAAGMARSVKSGSAALDEKQAAAKPASLALPGWIKRVWVAVALILGFTGMMQMPIAARY
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GLTRLPGMGWTGNFYTTLNIHYVAGAVLIALCCLLIALRLKAGGGFPRFVFWGAVRASLV
CCHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VGLVLTGIFRVLKNLPAFSFAPELVMGMDLAHLGLAAVLGALSLALWVSGRKAWTGPAR
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]