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The map label for this gene is rfbB [H]

Identifier: 220903817

GI number: 220903817

Start: 651533

End: 652624

Strand: Direct

Name: rfbB [H]

Synonym: Ddes_0540

Alternate gene names: 220903817

Gene position: 651533-652624 (Clockwise)

Preceding gene: 220903816

Following gene: 220903818

Centisome position: 22.67

GC content: 60.53

Gene sequence:

>1092_bases
ATGACCTGCCAACTTGTCACAGGGGGCGCGGGCTTCATCGGCTCGGCCTATGTGCTTCAGGCGCGCCGCGCCGGCATCCG
CGTCATCAATCTCGACAAGCTGACCTATGCGGGCAATCCGGCCAATCTGGCTTCACTGACCGGCGACCCGGAGCATGTTT
TCGTGCGCGGCGATATCGGCAATGAGGAGCTGGTAGCCTTTCTGCTCGAGACGCATCAGCCCGATGCCGTGGTCAATTTT
GCCGCAGAAAGCCATGTGGACCGCTCTATTGTGGCCCCGGACGCTTTTGCCCGTACCAACGTGCTCGGCACGGCAACCCT
GCTGCGTGTGGTCAAGGACTGGTGGAGCGGGCTTTCTTTTAAGCAGGCTGCGGATTTTCGTTTTCTGCATGTGTCCACAG
ATGAGGTGTACGGCGCTCTACAGCCCGGCGACCCGGCTTTTTCAGAGAGCACGCCATACAGTCCCAACAGCCCGTATTCG
GCGTCCAAGGCGGCCAGTGACCATATGGTGCGCGCCTTTCACGAAACCTACGGCCTACCCATCCTGCTGACCAACTGTTC
CAATAATTACGGGCCGCGTCAGTTTCCGGAAAAACTCATTCCTCTCATGATTCTTACCGCACTTGAGCGCAAACCCTTGC
CCGTGTACGGGCAGGGGGCCAATATCCGCGACTGGCTGCATGTGGACGACCACTGCACAGCCATCGCCCGGGTGCTTGAG
GCCGGGCAGGTAGGGCGCACCTACAATGTCGGCGGTCATGCGGAGCGGACCAACCTTGAGGTGGTGCATGCCGTGTGCGC
CATTCTTGATGACGTGCGCCCTTCCGCCCGGGGGCCGTATGCCGATCTCATCACCTTTGTTTCCGACAGGCCCGGGCATG
ACTTCCGTTATGCCATCGACTGCTCGCGCATTGAAAGCGAACTGGGCTGGCGGCCTTCGCGCCGGTTTGACACGGGGTTG
CGCGATACCGTGCGCTGGTATCTGGAAAACAGCGCCTGGGTAGATCTTGTGCGCAGCGGGGCCTATCTTGAGTGGATGGA
TATCAACTACGGGCGGCGTACCATTGCAGGCGGCGGGGGGGAAAGCCTATGA

Upstream 100 bases:

>100_bases
TTTTGATACCTTGCTGGTCACGTTGACGTGCTTACTTCTGCCCGGGTTTTGCAACACGCAGGGCTGCCATGAAAAAATTT
CAGATTAAAAGGATTCACCC

Downstream 100 bases:

>100_bases
GCGCATGGAAAGGCATTGTTCTTGCCGGGGGATCAGGTTCGCGCCTTTATCCCCTGACCCTGAGCGTGAGCAAGCAGCTC
ATGCCCATCTACGACAAGCC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTCQLVTGGAGFIGSAYVLQARRAGIRVINLDKLTYAGNPANLASLTGDPEHVFVRGDIGNEELVAFLLETHQPDAVVNF
AAESHVDRSIVAPDAFARTNVLGTATLLRVVKDWWSGLSFKQAADFRFLHVSTDEVYGALQPGDPAFSESTPYSPNSPYS
ASKAASDHMVRAFHETYGLPILLTNCSNNYGPRQFPEKLIPLMILTALERKPLPVYGQGANIRDWLHVDDHCTAIARVLE
AGQVGRTYNVGGHAERTNLEVVHAVCAILDDVRPSARGPYADLITFVSDRPGHDFRYAIDCSRIESELGWRPSRRFDTGL
RDTVRWYLENSAWVDLVRSGAYLEWMDINYGRRTIAGGGGESL

Sequences:

>Translated_363_residues
MTCQLVTGGAGFIGSAYVLQARRAGIRVINLDKLTYAGNPANLASLTGDPEHVFVRGDIGNEELVAFLLETHQPDAVVNF
AAESHVDRSIVAPDAFARTNVLGTATLLRVVKDWWSGLSFKQAADFRFLHVSTDEVYGALQPGDPAFSESTPYSPNSPYS
ASKAASDHMVRAFHETYGLPILLTNCSNNYGPRQFPEKLIPLMILTALERKPLPVYGQGANIRDWLHVDDHCTAIARVLE
AGQVGRTYNVGGHAERTNLEVVHAVCAILDDVRPSARGPYADLITFVSDRPGHDFRYAIDCSRIESELGWRPSRRFDTGL
RDTVRWYLENSAWVDLVRSGAYLEWMDINYGRRTIAGGGGESL
>Mature_362_residues
TCQLVTGGAGFIGSAYVLQARRAGIRVINLDKLTYAGNPANLASLTGDPEHVFVRGDIGNEELVAFLLETHQPDAVVNFA
AESHVDRSIVAPDAFARTNVLGTATLLRVVKDWWSGLSFKQAADFRFLHVSTDEVYGALQPGDPAFSESTPYSPNSPYSA
SKAASDHMVRAFHETYGLPILLTNCSNNYGPRQFPEKLIPLMILTALERKPLPVYGQGANIRDWLHVDDHCTAIARVLEA
GQVGRTYNVGGHAERTNLEVVHAVCAILDDVRPSARGPYADLITFVSDRPGHDFRYAIDCSRIESELGWRPSRRFDTGLR
DTVRWYLENSAWVDLVRSGAYLEWMDINYGRRTIAGGGGESL

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=35.3658536585366, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=23.780487804878, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI56237023, Length=358, Percent_Identity=27.6536312849162, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI56118217, Length=358, Percent_Identity=27.6536312849162, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI189083684, Length=358, Percent_Identity=27.6536312849162, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4504031, Length=360, Percent_Identity=25.2777777777778, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=53.3519553072626, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=54.6511627906977, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=29.6829971181556, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788366, Length=370, Percent_Identity=24.5945945945946, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=34.2342342342342, Blast_Score=203, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=31.7507418397626, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=328, Percent_Identity=24.6951219512195, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=247, Percent_Identity=25.9109311740891, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=247, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=247, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=247, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=247, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=247, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=392, Percent_Identity=26.530612244898, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=28.1767955801105, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=24.9258160237389, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39982; Mature: 39851

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCQLVTGGAGFIGSAYVLQARRAGIRVINLDKLTYAGNPANLASLTGDPEHVFVRGDIG
CEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCEEECCCCCCHHCCCCCCCEEEEECCCC
NEELVAFLLETHQPDAVVNFAAESHVDRSIVAPDAFARTNVLGTATLLRVVKDWWSGLSF
CHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KQAADFRFLHVSTDEVYGALQPGDPAFSESTPYSPNSPYSASKAASDHMVRAFHETYGLP
CCCCCEEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
ILLTNCSNNYGPRQFPEKLIPLMILTALERKPLPVYGQGANIRDWLHVDDHCTAIARVLE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHHHH
AGQVGRTYNVGGHAERTNLEVVHAVCAILDDVRPSARGPYADLITFVSDRPGHDFRYAID
HCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEE
CSRIESELGWRPSRRFDTGLRDTVRWYLENSAWVDLVRSGAYLEWMDINYGRRTIAGGGG
HHHHHHHHCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCEEEECCCC
ESL
CCC
>Mature Secondary Structure 
TCQLVTGGAGFIGSAYVLQARRAGIRVINLDKLTYAGNPANLASLTGDPEHVFVRGDIG
EEEEEECCCCHHHHHHHHHHHHCCEEEEEECCEEECCCCCCHHCCCCCCCEEEEECCCC
NEELVAFLLETHQPDAVVNFAAESHVDRSIVAPDAFARTNVLGTATLLRVVKDWWSGLSF
CHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KQAADFRFLHVSTDEVYGALQPGDPAFSESTPYSPNSPYSASKAASDHMVRAFHETYGLP
CCCCCEEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
ILLTNCSNNYGPRQFPEKLIPLMILTALERKPLPVYGQGANIRDWLHVDDHCTAIARVLE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHHHH
AGQVGRTYNVGGHAERTNLEVVHAVCAILDDVRPSARGPYADLITFVSDRPGHDFRYAID
HCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEE
CSRIESELGWRPSRRFDTGLRDTVRWYLENSAWVDLVRSGAYLEWMDINYGRRTIAGGGG
HHHHHHHHCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCEEEECCCC
ESL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]