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The map label for this gene is thrS

Identifier: 220903768

GI number: 220903768

Start: 598525

End: 600468

Strand: Reverse

Name: thrS

Synonym: Ddes_0490

Alternate gene names: 220903768

Gene position: 600468-598525 (Counterclockwise)

Preceding gene: 220903770

Following gene: 220903767

Centisome position: 20.9

GC content: 58.49

Gene sequence:

>1944_bases
ATGGAAGTCCGTGTGGAAGGGCAGATGGTAGAGGGGCAGGCTGGCGACAGCGTGGCCGCCATACTCCAGAAAGCCTTGAG
CGGCAAGAAGTTCAAGGCCGTTGTGGCCGCCCGCGCCCTTGACAGCGCGGAAGAACTGCTTGACCTTTCCTCTCCGGTAC
CCGCCGGTTGCACCGGCATCGAGCCGGTGTATGCCGACTCGCCCGAGGGCCTGCAGATACTCCGCCATTCCACCGCCCAC
GTCATGGCGGCGGCGGTGAAGCAGCTTTTTCCCAAAACCCGCGTTACCATCGGGCCTTCCATCGAATCCGGCTTTTACTA
CGACTTTGACGTGGAAAAGCCCTTTTCCAGCGAAGATTTTTCCGCCATTGAAGCGGAAATGCAGCGCATTGCCAATGCGC
GCGAACCCTTTACCCGCGAAGTGCTTTCCAGGGCTGAAGCCATAGAGCGCTTCAAGGCTATGGGCGAAGACTACAAGGTT
GAAATCATCGAAGGCATCGATGCAGATACGGTGTCGGTCTATACCTGCAGCGGCTTTGCAGACCTGTGCCGCGGCCCGCA
TGTGCCGCACACGGGCTTTGCCAAAGCCTCCAAGCTCATGAGCGTTGCCGGGGCCTACTGGCGCGGCGACGAAAAAAACC
GTATGCTGTCCCGAATTTACGGCACGGCCTTTGCGGACGAAAAAGCCCTTGCCGCCTACCTGAAGCAGATGGAAGAAGCC
AAACGCCGCGACCACCGCAAGCTGGGGCGCGAGCTTTCGCTCTTCACCTTCAAGGAAGACGTGGCCCCCGGCATGGTGTT
CTGGCTGCCCAAGGGCATGCTTGTGCGCACCATTCTGGAGGACTTCTGGCGCAAGGAGCACCTCAAGCGCGGGTATGATA
TAGTACAGGGGCCGCAGCTTCTGCGTGTTGAAACATGGCAGAAATCCGGCCATTACGACCATTACCGCGAAAACATGTAC
TTTACGCAGATCGAGGAAGACGCCTACGGTGTCAAACCGATGAACTGCATCTCGCACATGCTTATTTACGGGAACGAACT
GCACAGCTACCGCGACCTGCCGCAGCGCTATTTTGAGCTTGGCGTAGTACACCGCCACGAAAAAAGCGGCGTGCTCCACG
GTCTTTTGCGTGTACGCCAGTTCACCCAGGATGATGCCCACATCATCTGCGCGCCTGAACAGCTTGAAGGCGAGATTCTT
GAGGTCATACATCTTATCCGTGACCTTATGAATCTCTTCGGCTTTGAATACAAGGTGGCAGTATCCACCAGGCCCGAAAG
CAGCATCGGCACGGATGAAGCGTGGGAAATGGCCACCAGCGCCCTTGTGCAGGCAGTGGAAAAGGCCGGCCTGCCCTACA
CCATCAATGAGGGCGACGGCGCTTTTTACGGTCCCAAGATCGACGTGCGCCTGCTGGACTGCATCGGCCGCGAATGGCAG
TGCTCCACCATTCAGGTGGACTTTACCCTGCCCGAACGTTTTGATCTCACCTATGTGGGGCAGGACGGCGAACGCCACCG
CCCTGTCATGGTGCACCGCGCCATCATGGGTTCTCTTGAACGCTTCATCGGCATTCTTGTAGAAAACTTCGCCGGGGCGC
TGCCCACCTGGCTTGCGCCCGAGCAGGCGCGCCTGCTTACGGTGACCGAAGCCGGCGACGAAGCCGTCAGCAGCATGCTT
GAAGAGTTCAAGGCTCTGGGCATCCGCGCCCAGGCCGACACGCGCAATGAAAAACTGGGTTTCAAGGTGCGTGAGGCCCA
ACTGGCCAAAGTTCCATACATTCTTGTTGTGGGAGAAAAGGAAGTGCAGGCGGGCGGCGCCAATGTGCGCCTGCGCAATG
GAGACAACCTGGGGCTCAAGTCCGTAGCGGAAATCGCCGCACTCATACGCACGGACGCCGAAGAGCCTTTCAAACAAGGA
GGGATGCGCTATAGCTTCGCCTAA

Upstream 100 bases:

>100_bases
GAATGACCGGTATCGGCGCAGTGGGGAGGTTTACCTCCCCATTTTGTTTTGGCTTTTTTGTCTGGCCGGGCAGCGCCTCC
GGCGATGCAAGGAGATTGTC

Downstream 100 bases:

>100_bases
CATGAGATTCCGCCGCGACATGCCGCAAGACAGCGTACGTCGCAACGAAATGATCCGCGCCCGTGAAGTGCGCGTGATCG
CCGCCGATGGCGAGCAGCTT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MEVRVEGQMVEGQAGDSVAAILQKALSGKKFKAVVAARALDSAEELLDLSSPVPAGCTGIEPVYADSPEGLQILRHSTAH
VMAAAVKQLFPKTRVTIGPSIESGFYYDFDVEKPFSSEDFSAIEAEMQRIANAREPFTREVLSRAEAIERFKAMGEDYKV
EIIEGIDADTVSVYTCSGFADLCRGPHVPHTGFAKASKLMSVAGAYWRGDEKNRMLSRIYGTAFADEKALAAYLKQMEEA
KRRDHRKLGRELSLFTFKEDVAPGMVFWLPKGMLVRTILEDFWRKEHLKRGYDIVQGPQLLRVETWQKSGHYDHYRENMY
FTQIEEDAYGVKPMNCISHMLIYGNELHSYRDLPQRYFELGVVHRHEKSGVLHGLLRVRQFTQDDAHIICAPEQLEGEIL
EVIHLIRDLMNLFGFEYKVAVSTRPESSIGTDEAWEMATSALVQAVEKAGLPYTINEGDGAFYGPKIDVRLLDCIGREWQ
CSTIQVDFTLPERFDLTYVGQDGERHRPVMVHRAIMGSLERFIGILVENFAGALPTWLAPEQARLLTVTEAGDEAVSSML
EEFKALGIRAQADTRNEKLGFKVREAQLAKVPYILVVGEKEVQAGGANVRLRNGDNLGLKSVAEIAALIRTDAEEPFKQG
GMRYSFA

Sequences:

>Translated_647_residues
MEVRVEGQMVEGQAGDSVAAILQKALSGKKFKAVVAARALDSAEELLDLSSPVPAGCTGIEPVYADSPEGLQILRHSTAH
VMAAAVKQLFPKTRVTIGPSIESGFYYDFDVEKPFSSEDFSAIEAEMQRIANAREPFTREVLSRAEAIERFKAMGEDYKV
EIIEGIDADTVSVYTCSGFADLCRGPHVPHTGFAKASKLMSVAGAYWRGDEKNRMLSRIYGTAFADEKALAAYLKQMEEA
KRRDHRKLGRELSLFTFKEDVAPGMVFWLPKGMLVRTILEDFWRKEHLKRGYDIVQGPQLLRVETWQKSGHYDHYRENMY
FTQIEEDAYGVKPMNCISHMLIYGNELHSYRDLPQRYFELGVVHRHEKSGVLHGLLRVRQFTQDDAHIICAPEQLEGEIL
EVIHLIRDLMNLFGFEYKVAVSTRPESSIGTDEAWEMATSALVQAVEKAGLPYTINEGDGAFYGPKIDVRLLDCIGREWQ
CSTIQVDFTLPERFDLTYVGQDGERHRPVMVHRAIMGSLERFIGILVENFAGALPTWLAPEQARLLTVTEAGDEAVSSML
EEFKALGIRAQADTRNEKLGFKVREAQLAKVPYILVVGEKEVQAGGANVRLRNGDNLGLKSVAEIAALIRTDAEEPFKQG
GMRYSFA
>Mature_647_residues
MEVRVEGQMVEGQAGDSVAAILQKALSGKKFKAVVAARALDSAEELLDLSSPVPAGCTGIEPVYADSPEGLQILRHSTAH
VMAAAVKQLFPKTRVTIGPSIESGFYYDFDVEKPFSSEDFSAIEAEMQRIANAREPFTREVLSRAEAIERFKAMGEDYKV
EIIEGIDADTVSVYTCSGFADLCRGPHVPHTGFAKASKLMSVAGAYWRGDEKNRMLSRIYGTAFADEKALAAYLKQMEEA
KRRDHRKLGRELSLFTFKEDVAPGMVFWLPKGMLVRTILEDFWRKEHLKRGYDIVQGPQLLRVETWQKSGHYDHYRENMY
FTQIEEDAYGVKPMNCISHMLIYGNELHSYRDLPQRYFELGVVHRHEKSGVLHGLLRVRQFTQDDAHIICAPEQLEGEIL
EVIHLIRDLMNLFGFEYKVAVSTRPESSIGTDEAWEMATSALVQAVEKAGLPYTINEGDGAFYGPKIDVRLLDCIGREWQ
CSTIQVDFTLPERFDLTYVGQDGERHRPVMVHRAIMGSLERFIGILVENFAGALPTWLAPEQARLLTVTEAGDEAVSSML
EEFKALGIRAQADTRNEKLGFKVREAQLAKVPYILVVGEKEVQAGGANVRLRNGDNLGLKSVAEIAALIRTDAEEPFKQG
GMRYSFA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=649, Percent_Identity=40.2157164869029, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI38202255, Length=583, Percent_Identity=42.3670668953688, Blast_Score=477, Evalue=1e-134,
Organism=Homo sapiens, GI20070344, Length=633, Percent_Identity=37.2827804107425, Blast_Score=408, Evalue=1e-114,
Organism=Escherichia coli, GI1788013, Length=597, Percent_Identity=47.571189279732, Blast_Score=577, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71984192, Length=638, Percent_Identity=37.7742946708464, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984184, Length=638, Percent_Identity=37.7742946708464, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=615, Percent_Identity=38.6991869918699, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322654, Length=385, Percent_Identity=36.8831168831169, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24583842, Length=584, Percent_Identity=41.7808219178082, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI28574141, Length=584, Percent_Identity=41.7808219178082, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24583839, Length=584, Percent_Identity=41.7808219178082, Blast_Score=495, Evalue=1e-140,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_DESDA (B8J4E4)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479080.1
- ProteinModelPortal:   B8J4E4
- GeneID:   7284155
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0490
- HOGENOM:   HBG352811
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72507; Mature: 72507

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRVEGQMVEGQAGDSVAAILQKALSGKKFKAVVAARALDSAEELLDLSSPVPAGCTGI
CEEEECCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPVYADSPEGLQILRHSTAHVMAAAVKQLFPKTRVTIGPSIESGFYYDFDVEKPFSSEDF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCHH
SAIEAEMQRIANAREPFTREVLSRAEAIERFKAMGEDYKVEIIEGIDADTVSVYTCSGFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCHH
DLCRGPHVPHTGFAKASKLMSVAGAYWRGDEKNRMLSRIYGTAFADEKALAAYLKQMEEA
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRRDHRKLGRELSLFTFKEDVAPGMVFWLPKGMLVRTILEDFWRKEHLKRGYDIVQGPQL
HHHHHHHHCCHHEEEEEHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCE
LRVETWQKSGHYDHYRENMYFTQIEEDAYGVKPMNCISHMLIYGNELHSYRDLPQRYFEL
EEEEEHHCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GVVHRHEKSGVLHGLLRVRQFTQDDAHIICAPEQLEGEILEVIHLIRDLMNLFGFEYKVA
HHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VSTRPESSIGTDEAWEMATSALVQAVEKAGLPYTINEGDGAFYGPKIDVRLLDCIGREWQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEHHHHCCCCEE
CSTIQVDFTLPERFDLTYVGQDGERHRPVMVHRAIMGSLERFIGILVENFAGALPTWLAP
CEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EQARLLTVTEAGDEAVSSMLEEFKALGIRAQADTRNEKLGFKVREAQLAKVPYILVVGEK
CCCEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCEEHHHHHCCCCEEEEECCH
EVQAGGANVRLRNGDNLGLKSVAEIAALIRTDAEEPFKQGGMRYSFA
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEECCC
>Mature Secondary Structure
MEVRVEGQMVEGQAGDSVAAILQKALSGKKFKAVVAARALDSAEELLDLSSPVPAGCTGI
CEEEECCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPVYADSPEGLQILRHSTAHVMAAAVKQLFPKTRVTIGPSIESGFYYDFDVEKPFSSEDF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCHH
SAIEAEMQRIANAREPFTREVLSRAEAIERFKAMGEDYKVEIIEGIDADTVSVYTCSGFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCHH
DLCRGPHVPHTGFAKASKLMSVAGAYWRGDEKNRMLSRIYGTAFADEKALAAYLKQMEEA
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRRDHRKLGRELSLFTFKEDVAPGMVFWLPKGMLVRTILEDFWRKEHLKRGYDIVQGPQL
HHHHHHHHCCHHEEEEEHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCE
LRVETWQKSGHYDHYRENMYFTQIEEDAYGVKPMNCISHMLIYGNELHSYRDLPQRYFEL
EEEEEHHCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GVVHRHEKSGVLHGLLRVRQFTQDDAHIICAPEQLEGEILEVIHLIRDLMNLFGFEYKVA
HHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VSTRPESSIGTDEAWEMATSALVQAVEKAGLPYTINEGDGAFYGPKIDVRLLDCIGREWQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEHHHHCCCCEE
CSTIQVDFTLPERFDLTYVGQDGERHRPVMVHRAIMGSLERFIGILVENFAGALPTWLAP
CEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EQARLLTVTEAGDEAVSSMLEEFKALGIRAQADTRNEKLGFKVREAQLAKVPYILVVGEK
CCCEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCEEHHHHHCCCCEEEEECCH
EVQAGGANVRLRNGDNLGLKSVAEIAALIRTDAEEPFKQGGMRYSFA
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA