Click here to switch to the map view.

The map label for this gene is braC [H]

Identifier: 220903750

GI number: 220903750

Start: 580130

End: 581251

Strand: Direct

Name: braC [H]

Synonym: Ddes_0472

Alternate gene names: 220903750

Gene position: 580130-581251 (Clockwise)

Preceding gene: 220903745

Following gene: 220903751

Centisome position: 20.19

GC content: 59.89

Gene sequence:

>1122_bases
ATGAAAGCACTTGGTCGTTTGCTTGGGGCGCTGGCCCTTACCCTGCTGGTGCCTATGACGGCTCTGGCCGGTGACATCAA
GGTCGGTCTCATGTGCCCGTTGACGGGCAAATGGGCTTCCGAAGGTCAGGACATGAAGAATATCGTCAGCCTGCTGGCTG
ATGAAGTGAACGCCAAAGGCGGCATCAATGGCCGCCAGATCAAGGTTGTGGTGGAAGACGACGCGGGCGACCCGCGCACC
GCTGCCCTTGCCGCGCAAAAGCTCGCCTCAGCGGGTGTGGTGGCTGTTATCGGTACCTACGGCTCGGCCGTAACCGAAGC
CAGCCAGAATATTCTGGACGAAGCCGGACTGGTACAGATCGGCACGGGTTCTACCAGTGTGCGACTGACGGAAAAGGGTC
TGCCGCTCTTCTTCCGTACTTGCCCGCGTGACGACGCCCAGGGCCGCGCGGCGGCCGATGCCGTCATCAAGGGCGATTAC
AAGGCCGTGGCGATTCTGCACGACAATTCCTCTTACGCCAAGGGCCTGGCCGAAGAAACCAGGGCCGCCCTGAAAAAAGA
CGGCGTAAAGGTGGTTTTTTATGACGCCCTCACCCCCGGAGAGCGCGATTACACGGCTATTCTGACCAAGCTCAAGGCTG
CCGGTCCCGATCTTGTGTTCTTTACCGGCTACTACCCTGAAACGGGCATGCTGCTGCGCCAGAAAAAGGAAATGGGCTGG
CCCGTGCCCATGATGGGCGGCGATGCCGCCAACCATCAGGATCTGGTGAAAATAGCGGGCAACGAGGCGGCCGAAGGCTA
CTTCTTTATCAGCCCGCCCCTGCCGCAGGATATGGACACCGCTGAAGCCCGGTCTTTCCTTGAATCCTTCAAGGCCAGGT
ATGATGCTGTGCCTGTTTCCGTGTGGGCCGTGCTGGCGGGCGACGCCTTCAAGGTGATTGAGGCCGCGCTGGCCGCAGGC
AACGACAAGCCCGAAAGCATGGCCGCGTGGCTCAAGAATCTCAAGGATATGCCGGGGCTTTCCGGCAGCCTGGGTTTTGA
CGCCAAGGGCGACCGCGTGGGCGAATTCTATCGTACCTATACGGTCAACGATAAGGGCGTATTTGTCTTGCAGCCCAAGT
AA

Upstream 100 bases:

>100_bases
CCGGATTTGGGGCGGTAAAACTGGGGCTTTTTCTTTACGGGAATTTGAGGTATGCTATTTTGTTACTATCATCACCGAAT
ACCGGACGGGGGAGTTTTTT

Downstream 100 bases:

>100_bases
ACAGGCCGTACAGTGTGCTCCGCGCCCCGGCGCGGTCCGGCGGTTTTTTGCCGCATACCCGCGCGCCGAAAATGGTACGC
GCATACACAGCAACTTTGGC

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKALGRLLGALALTLLVPMTALAGDIKVGLMCPLTGKWASEGQDMKNIVSLLADEVNAKGGINGRQIKVVVEDDAGDPRT
AALAAQKLASAGVVAVIGTYGSAVTEASQNILDEAGLVQIGTGSTSVRLTEKGLPLFFRTCPRDDAQGRAAADAVIKGDY
KAVAILHDNSSYAKGLAEETRAALKKDGVKVVFYDALTPGERDYTAILTKLKAAGPDLVFFTGYYPETGMLLRQKKEMGW
PVPMMGGDAANHQDLVKIAGNEAAEGYFFISPPLPQDMDTAEARSFLESFKARYDAVPVSVWAVLAGDAFKVIEAALAAG
NDKPESMAAWLKNLKDMPGLSGSLGFDAKGDRVGEFYRTYTVNDKGVFVLQPK

Sequences:

>Translated_373_residues
MKALGRLLGALALTLLVPMTALAGDIKVGLMCPLTGKWASEGQDMKNIVSLLADEVNAKGGINGRQIKVVVEDDAGDPRT
AALAAQKLASAGVVAVIGTYGSAVTEASQNILDEAGLVQIGTGSTSVRLTEKGLPLFFRTCPRDDAQGRAAADAVIKGDY
KAVAILHDNSSYAKGLAEETRAALKKDGVKVVFYDALTPGERDYTAILTKLKAAGPDLVFFTGYYPETGMLLRQKKEMGW
PVPMMGGDAANHQDLVKIAGNEAAEGYFFISPPLPQDMDTAEARSFLESFKARYDAVPVSVWAVLAGDAFKVIEAALAAG
NDKPESMAAWLKNLKDMPGLSGSLGFDAKGDRVGEFYRTYTVNDKGVFVLQPK
>Mature_373_residues
MKALGRLLGALALTLLVPMTALAGDIKVGLMCPLTGKWASEGQDMKNIVSLLADEVNAKGGINGRQIKVVVEDDAGDPRT
AALAAQKLASAGVVAVIGTYGSAVTEASQNILDEAGLVQIGTGSTSVRLTEKGLPLFFRTCPRDDAQGRAAADAVIKGDY
KAVAILHDNSSYAKGLAEETRAALKKDGVKVVFYDALTPGERDYTAILTKLKAAGPDLVFFTGYYPETGMLLRQKKEMGW
PVPMMGGDAANHQDLVKIAGNEAAEGYFFISPPLPQDMDTAEARSFLESFKARYDAVPVSVWAVLAGDAFKVIEAALAAG
NDKPESMAAWLKNLKDMPGLSGSLGFDAKGDRVGEFYRTYTVNDKGVFVLQPK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=34.3490304709141, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789867, Length=357, Percent_Identity=33.6134453781513, Blast_Score=174, Evalue=7e-45,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39542; Mature: 39542

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALGRLLGALALTLLVPMTALAGDIKVGLMCPLTGKWASEGQDMKNIVSLLADEVNAKG
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCHHHHHHHHHHHHCCCC
GINGRQIKVVVEDDAGDPRTAALAAQKLASAGVVAVIGTYGSAVTEASQNILDEAGLVQI
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEE
GTGSTSVRLTEKGLPLFFRTCPRDDAQGRAAADAVIKGDYKAVAILHDNSSYAKGLAEET
CCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHEEECCCEEEEEEECCCHHHHHHHHHH
RAALKKDGVKVVFYDALTPGERDYTAILTKLKAAGPDLVFFTGYYPETGMLLRQKKEMGW
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
PVPMMGGDAANHQDLVKIAGNEAAEGYFFISPPLPQDMDTAEARSFLESFKARYDAVPVS
CCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
VWAVLAGDAFKVIEAALAAGNDKPESMAAWLKNLKDMPGLSGSLGFDAKGDRVGEFYRTY
HHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCEEEEE
TVNDKGVFVLQPK
EECCCEEEEECCC
>Mature Secondary Structure
MKALGRLLGALALTLLVPMTALAGDIKVGLMCPLTGKWASEGQDMKNIVSLLADEVNAKG
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCHHHHHHHHHHHHCCCC
GINGRQIKVVVEDDAGDPRTAALAAQKLASAGVVAVIGTYGSAVTEASQNILDEAGLVQI
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEE
GTGSTSVRLTEKGLPLFFRTCPRDDAQGRAAADAVIKGDYKAVAILHDNSSYAKGLAEET
CCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHEEECCCEEEEEEECCCHHHHHHHHHH
RAALKKDGVKVVFYDALTPGERDYTAILTKLKAAGPDLVFFTGYYPETGMLLRQKKEMGW
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
PVPMMGGDAANHQDLVKIAGNEAAEGYFFISPPLPQDMDTAEARSFLESFKARYDAVPVS
CCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
VWAVLAGDAFKVIEAALAAGNDKPESMAAWLKNLKDMPGLSGSLGFDAKGDRVGEFYRTY
HHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCEEEEE
TVNDKGVFVLQPK
EECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]