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The map label for this gene is thiM

Identifier: 220903745

GI number: 220903745

Start: 568689

End: 569546

Strand: Direct

Name: thiM

Synonym: Ddes_0467

Alternate gene names: 220903745

Gene position: 568689-569546 (Clockwise)

Preceding gene: 220903744

Following gene: 220903750

Centisome position: 19.79

GC content: 61.89

Gene sequence:

>858_bases
ATGACAGTTTTTGCGGATATTCTTGATACAGTGCGGCGTCATGAGCCGGTGGTGCACTGCATAACGAACTACGTCACGGT
TAACGACTGCGCCAATATGGTTCTGGCGGCGGGCGGATCGCCCATTATGGCCGACGATGCGGCGGAAGTGGAAGATATTG
TGGCCCTGTCGCAGGCGCTTGTGATCAATATAGGCACGCTCAATAGCCGCACGGTGGAATCAATGCTGGTCGCGGGCAGG
CGGGCCAACAGTATGGGCAAGCCCGTGGTGCTTGATCCTGTGGGTGCGGGGGCCTCGGCCCTGCGCAACAGCACCTTGCA
ACGGTTGCTGGGCGAAGTCCGTTTTGCGGCCATCAAGGGCAATGCCTCGGAAATAGGTTTTCTGGCCGGCAAAGATGCAA
AGGCCAGGGGTGTAGATGCCCAGGACTCTTGCCTTGTGACCGAAGACAGGCTTGAAAGCGCGGCAGCTACGGCCCGCCAC
TTGAGCGAAAGCACGGGAGCAGTGGTGGTTGTCAGCGGAGCCATTGATATCGTGGCGTATTCCGGTGGCGCTTGGGCCGT
GCGCAACGGGCATCCGCTCATGGCGCGTATCACGGGGTCCGGCTGCATGTCGGCTGCGGTTATGGGGTGCTGCCTTGGCG
TAGCGCCGTATGAAATGCCCCAGGCGTGCCTGTGCGCTGTCAGCGCTCTGGGGGTTGCCGGAGAGATCGCCGCTGAAACT
ATGGTAGGTGGCGGCGGGGAGATGGGCGGCCTGGGCGGCGGCACTGGCAGTTACCGGGCACTGCTGCTGGACGCCATGAG
TCTGCTTGACGGGCTGGCGCTTGGCAGCCGGGCTGATGTGACACGCATCCAGATGTAG

Upstream 100 bases:

>100_bases
GGGGCTGTGTGCCGTGCCGGTACAGGGCGCATGTTTGCCGTTTGTGTTGCGGGCGGCTCGAGACTTCCGGCGATGCATTG
TTTTGCCGGAAGGAGGTAGG

Downstream 100 bases:

>100_bases
AGAATTTTTACGTTGAAAAAGGTTGAATGCTTAGAGGGTCTGACGTTTGAGGTGATTTTAATAAAAGCCCTTTCCTTGCA
GTCAGGCTGCCGCCCTGCGG

Product: Hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVFADILDTVRRHEPVVHCITNYVTVNDCANMVLAAGGSPIMADDAAEVEDIVALSQALVINIGTLNSRTVESMLVAGR
RANSMGKPVVLDPVGAGASALRNSTLQRLLGEVRFAAIKGNASEIGFLAGKDAKARGVDAQDSCLVTEDRLESAAATARH
LSESTGAVVVVSGAIDIVAYSGGAWAVRNGHPLMARITGSGCMSAAVMGCCLGVAPYEMPQACLCAVSALGVAGEIAAET
MVGGGGEMGGLGGGTGSYRALLLDAMSLLDGLALGSRADVTRIQM

Sequences:

>Translated_285_residues
MTVFADILDTVRRHEPVVHCITNYVTVNDCANMVLAAGGSPIMADDAAEVEDIVALSQALVINIGTLNSRTVESMLVAGR
RANSMGKPVVLDPVGAGASALRNSTLQRLLGEVRFAAIKGNASEIGFLAGKDAKARGVDAQDSCLVTEDRLESAAATARH
LSESTGAVVVVSGAIDIVAYSGGAWAVRNGHPLMARITGSGCMSAAVMGCCLGVAPYEMPQACLCAVSALGVAGEIAAET
MVGGGGEMGGLGGGTGSYRALLLDAMSLLDGLALGSRADVTRIQM
>Mature_284_residues
TVFADILDTVRRHEPVVHCITNYVTVNDCANMVLAAGGSPIMADDAAEVEDIVALSQALVINIGTLNSRTVESMLVAGRR
ANSMGKPVVLDPVGAGASALRNSTLQRLLGEVRFAAIKGNASEIGFLAGKDAKARGVDAQDSCLVTEDRLESAAATARHL
SESTGAVVVVSGAIDIVAYSGGAWAVRNGHPLMARITGSGCMSAAVMGCCLGVAPYEMPQACLCAVSALGVAGEIAAETM
VGGGGEMGGLGGGTGSYRALLLDAMSLLDGLALGSRADVTRIQM

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=208, Percent_Identity=39.9038461538462, Blast_Score=128, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325042, Length=292, Percent_Identity=27.7397260273973, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_DESDA (B8J4C1)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479057.1
- ProteinModelPortal:   B8J4C1
- GeneID:   7284132
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0467
- HOGENOM:   HBG351126
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28924; Mature: 28792

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00318 HMG_COA_REDUCTASE_2

Important sites: BINDING 43-43 BINDING 119-119 BINDING 172-172 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFADILDTVRRHEPVVHCITNYVTVNDCANMVLAAGGSPIMADDAAEVEDIVALSQAL
CCCHHHHHHHHHHCCCHHHHHHCCEEHHHHHHHHEECCCCCEECCCHHHHHHHHHHHHHH
VINIGTLNSRTVESMLVAGRRANSMGKPVVLDPVGAGASALRNSTLQRLLGEVRFAAIKG
EEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEC
NASEIGFLAGKDAKARGVDAQDSCLVTEDRLESAAATARHLSESTGAVVVVSGAIDIVAY
CCCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEE
SGGAWAVRNGHPLMARITGSGCMSAAVMGCCLGVAPYEMPQACLCAVSALGVAGEIAAET
CCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHH
MVGGGGEMGGLGGGTGSYRALLLDAMSLLDGLALGSRADVTRIQM
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
TVFADILDTVRRHEPVVHCITNYVTVNDCANMVLAAGGSPIMADDAAEVEDIVALSQAL
CCHHHHHHHHHHCCCHHHHHHCCEEHHHHHHHHEECCCCCEECCCHHHHHHHHHHHHHH
VINIGTLNSRTVESMLVAGRRANSMGKPVVLDPVGAGASALRNSTLQRLLGEVRFAAIKG
EEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEC
NASEIGFLAGKDAKARGVDAQDSCLVTEDRLESAAATARHLSESTGAVVVVSGAIDIVAY
CCCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEE
SGGAWAVRNGHPLMARITGSGCMSAAVMGCCLGVAPYEMPQACLCAVSALGVAGEIAAET
CCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHH
MVGGGGEMGGLGGGTGSYRALLLDAMSLLDGLALGSRADVTRIQM
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA