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The map label for this gene is proA

Identifier: 220903719

GI number: 220903719

Start: 532874

End: 534145

Strand: Reverse

Name: proA

Synonym: Ddes_0441

Alternate gene names: 220903719

Gene position: 534145-532874 (Counterclockwise)

Preceding gene: 220903723

Following gene: 220903718

Centisome position: 18.59

GC content: 62.66

Gene sequence:

>1272_bases
ATGACGCCGGCTGAAGAAATGGCGCGTCTTGGCGCGCAGGCAAAGGATGCGGCGCGGGCCATGACCAGGGCCACGCCCGA
AGCCAAGAACCAGGCCCTGCTGGGCCTGGCGCAACTGCTGCATCAGCGCGAACCGGAAATTCTGGCCGCCAACGCCCGCG
ATATTGAAGCTGCCCGGGCCGCCGGGCAGGATGCGGCACGGCTGGACCGCCTTACGCTGACTTCGGCCATTATGGAAGAA
ATGCGCGCCGCCTGCGCCCATGTGGCCAACCTGCCAGACCCTGTCGGCGCTACCGAAAGCCAGTGGCAACGGCCCAACGG
CCTGCTGGTAGGCAAGATGCGCATACCGCTCGGCGTGATCGCCATGGTTTACGAGGCCCGCCCCAATGTAACCATTGATG
CGGCCATCCTGTGCATCAAGGCGGGTAATGCCGTTATTCTGCGTGGCGGCAGCGAGGCCATACACTCCAACACAGCCCTG
GCCCAGACCCTGCAAGAAGCCATGGTCCAGGCCGGGCTGCCCGCTTCGGCGGCGCAGCTTGTGACGGTCCCGGGGCATGA
GGCTGTGAACGCCCTGTGCAAGCTTGACCAGTATATTGACGTGATCATCCCCCGCGGCGGCGAAAGGCTTGTACGCGCGG
TTACCGAAGCCGCCACCATGCCCGTGCTCAAGCACTTCAAGGGCGTGTGCCACGCCTATATCGAGCCGGACGCCGACCTT
GAGCGCGCGCTGGATATTGTTTTTAACGGCAAGGTGCAGCGGCCAGGCGTATGTAACGCCCTGGAATGCCTGCTTGTACA
CAAGGATGCAGCCTCGGCCTTTCTGCCCCTGGTGGCGGAAAAACTCGGCGCGGCGGGCGTGGAATTTCGGGCTGACACCA
CGGCCCTGCCCCTTATGAACAAGGCCCCGCAAGGCCGCGTTGTTCCCCAGCGGGCTGAAGACCTTGGTCAGGAATTTCAT
GATCTTGTTCTCGCCGTTCGCGTGGTGGACAGTATGGATGAAGCGCTGGACCACATTGCCCGCTATGGATCCAACCATAC
AGAAATCATCTGCACCAATGACTATGCCAAGGCCATGCGCTTTCTGCGCGAAGCCGATGCCTCCATGGTGGCGGTCAATG
CATCCAGCCGCTTCAACGACGGCGGGCAGCTCGGCCTGGGCGCGGAGATCGGCATATCCACCTCCAAGCTGCATGCTTAC
GGGGCCATGGGCGTTGAAGAACTGACCACCACCAAATTTGTGGTGTTGGGGCAGGGGCAGGTGCGGCAGTAG

Upstream 100 bases:

>100_bases
AGTCTTCTTCATAGGCCATAAAACGTCCATCTGTCAAAACAAATATCCCCCGGCCAGAGCCGCAAAGGCGCGCTCATGGC
CGCTGCCACAAGGAGCTTGC

Downstream 100 bases:

>100_bases
TGACCATACGGCGCACTCTGCGGCCCACAGCAGACTCTGCAAGCCCGCCCACGGGACGGGCCATCCTGGGGGGCAGTTTT
AATCCGCCCCACGTGGGACA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTPAEEMARLGAQAKDAARAMTRATPEAKNQALLGLAQLLHQREPEILAANARDIEAARAAGQDAARLDRLTLTSAIMEE
MRAACAHVANLPDPVGATESQWQRPNGLLVGKMRIPLGVIAMVYEARPNVTIDAAILCIKAGNAVILRGGSEAIHSNTAL
AQTLQEAMVQAGLPASAAQLVTVPGHEAVNALCKLDQYIDVIIPRGGERLVRAVTEAATMPVLKHFKGVCHAYIEPDADL
ERALDIVFNGKVQRPGVCNALECLLVHKDAASAFLPLVAEKLGAAGVEFRADTTALPLMNKAPQGRVVPQRAEDLGQEFH
DLVLAVRVVDSMDEALDHIARYGSNHTEIICTNDYAKAMRFLREADASMVAVNASSRFNDGGQLGLGAEIGISTSKLHAY
GAMGVEELTTTKFVVLGQGQVRQ

Sequences:

>Translated_423_residues
MTPAEEMARLGAQAKDAARAMTRATPEAKNQALLGLAQLLHQREPEILAANARDIEAARAAGQDAARLDRLTLTSAIMEE
MRAACAHVANLPDPVGATESQWQRPNGLLVGKMRIPLGVIAMVYEARPNVTIDAAILCIKAGNAVILRGGSEAIHSNTAL
AQTLQEAMVQAGLPASAAQLVTVPGHEAVNALCKLDQYIDVIIPRGGERLVRAVTEAATMPVLKHFKGVCHAYIEPDADL
ERALDIVFNGKVQRPGVCNALECLLVHKDAASAFLPLVAEKLGAAGVEFRADTTALPLMNKAPQGRVVPQRAEDLGQEFH
DLVLAVRVVDSMDEALDHIARYGSNHTEIICTNDYAKAMRFLREADASMVAVNASSRFNDGGQLGLGAEIGISTSKLHAY
GAMGVEELTTTKFVVLGQGQVRQ
>Mature_422_residues
TPAEEMARLGAQAKDAARAMTRATPEAKNQALLGLAQLLHQREPEILAANARDIEAARAAGQDAARLDRLTLTSAIMEEM
RAACAHVANLPDPVGATESQWQRPNGLLVGKMRIPLGVIAMVYEARPNVTIDAAILCIKAGNAVILRGGSEAIHSNTALA
QTLQEAMVQAGLPASAAQLVTVPGHEAVNALCKLDQYIDVIIPRGGERLVRAVTEAATMPVLKHFKGVCHAYIEPDADLE
RALDIVFNGKVQRPGVCNALECLLVHKDAASAFLPLVAEKLGAAGVEFRADTTALPLMNKAPQGRVVPQRAEDLGQEFHD
LVLAVRVVDSMDEALDHIARYGSNHTEIICTNDYAKAMRFLREADASMVAVNASSRFNDGGQLGLGAEIGISTSKLHAYG
AMGVEELTTTKFVVLGQGQVRQ

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=414, Percent_Identity=37.6811594202899, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI62912457, Length=414, Percent_Identity=37.6811594202899, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=42.7884615384615, Blast_Score=335, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17569969, Length=422, Percent_Identity=36.7298578199052, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=422, Percent_Identity=36.7298578199052, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324899, Length=437, Percent_Identity=32.4942791762014, Blast_Score=230, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21357643, Length=419, Percent_Identity=38.9021479713604, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_DESDA (B8J495)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479031.1
- ProteinModelPortal:   B8J495
- GeneID:   7284106
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0441
- HOGENOM:   HBG318080
- ProtClustDB:   PRK00197
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45076; Mature: 44945

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAEEMARLGAQAKDAARAMTRATPEAKNQALLGLAQLLHQREPEILAANARDIEAARA
CCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
AGQDAARLDRLTLTSAIMEEMRAACAHVANLPDPVGATESQWQRPNGLLVGKMRIPLGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCHHHH
AMVYEARPNVTIDAAILCIKAGNAVILRGGSEAIHSNTALAQTLQEAMVQAGLPASAAQL
HHHHHCCCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VTVPGHEAVNALCKLDQYIDVIIPRGGERLVRAVTEAATMPVLKHFKGVCHAYIEPDADL
EECCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ERALDIVFNGKVQRPGVCNALECLLVHKDAASAFLPLVAEKLGAAGVEFRADTTALPLMN
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCC
KAPQGRVVPQRAEDLGQEFHDLVLAVRVVDSMDEALDHIARYGSNHTEIICTNDYAKAMR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHH
FLREADASMVAVNASSRFNDGGQLGLGAEIGISTSKLHAYGAMGVEELTTTKFVVLGQGQ
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCEEEEEECCCC
VRQ
CCC
>Mature Secondary Structure 
TPAEEMARLGAQAKDAARAMTRATPEAKNQALLGLAQLLHQREPEILAANARDIEAARA
CCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
AGQDAARLDRLTLTSAIMEEMRAACAHVANLPDPVGATESQWQRPNGLLVGKMRIPLGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCHHHH
AMVYEARPNVTIDAAILCIKAGNAVILRGGSEAIHSNTALAQTLQEAMVQAGLPASAAQL
HHHHHCCCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VTVPGHEAVNALCKLDQYIDVIIPRGGERLVRAVTEAATMPVLKHFKGVCHAYIEPDADL
EECCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ERALDIVFNGKVQRPGVCNALECLLVHKDAASAFLPLVAEKLGAAGVEFRADTTALPLMN
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCC
KAPQGRVVPQRAEDLGQEFHDLVLAVRVVDSMDEALDHIARYGSNHTEIICTNDYAKAMR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHH
FLREADASMVAVNASSRFNDGGQLGLGAEIGISTSKLHAYGAMGVEELTTTKFVVLGQGQ
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCEEEEEECCCC
VRQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA