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The map label for this gene is rlmN [H]

Identifier: 220903723

GI number: 220903723

Start: 537852

End: 539012

Strand: Reverse

Name: rlmN [H]

Synonym: Ddes_0445

Alternate gene names: 220903723

Gene position: 539012-537852 (Counterclockwise)

Preceding gene: 220903724

Following gene: 220903719

Centisome position: 18.76

GC content: 57.88

Gene sequence:

>1161_bases
ATGTCCGGGCCGTTTGCGCAGGGCGCGGCCGCAACCGTAACACAGGATGAACAGTCCGGCAGGACCATGACAAATCTTCT
CAATCTCACACTTCCCGAACTGGAAGCCTGGACGCAGTCAGAACTGGGCGAACCCAAATTTCGCGCCATGCAACTGTGGC
AGTGGATATGGCAGCGCATGGCGCGCGATTTCGACACCATGACCAACATATCCCGCCCCTGCCGCGAGCTTCTGGCCGCC
AAGGCCTGTATCGTCTGGCCGGAAGTCAGTGCTGTGGAAGAAAGCCGGGACGGCACCACAAAATTTCTGCTGCGCCTTGA
AGACGGCGCGCAAATTGAGACCGTGCTTATCCCTTCTGATTCGCGCGAAGGCGTGCGCCGCTGGACGCAATGCCTCTCCT
GCCAGGTGGGGTGCACCATGGGCTGCACCTTCTGCTCCACAGGACAAATGGGCTTTGAGCGCAACATGACCATGGGAGAA
ATCCTCGGCCAGATTCTGGTGGCCCGGGAACATCTGGGCGATACCCGCCTGCACTGGCCCATACTGCGCAACCTTGTTTT
CATGGGTATGGGTGAACCCCTGCTGAACCTGAAAGAAGTCATGCGCTCACTCGAAAGCCTCAATAACGACAAGGGACTGG
GATTTTCGCCCCGCCGCATCACGGTTTCCACCTGCGGCATTGAAAAGGGCCTGAAAGAGCTGGGCGAATCCGGGCTAGCC
TTTCTGGCCGTATCGCTGCACGCCCCCAACCAAGCGGTGCGCGAGCGCATTATGCCCAAGGCCGCCCGCTGGCGACTGAA
TGATCTCATGGCAGCACTCAAGTCCTATCCACTCAAAACGCGCGAACACATCACTTTTGAATACTTGATGCTCGGCGGTG
TCAACGACAGTCTGGAACACGCCAGAGAACTGGCCCCCCTGGTCAGTGCGGTCAAGGGCAAGTTGAACATCATCGTGTAC
AACGCTGCAGAAGGCTCCCCTTACCAGGCGCCCAGTGAAGAACGCATACTGGCGTTTGAAAAATACCTCTGGAGCAAAGA
TATCACTGCCATTATCCGCAAAAGCAAAGGGCAGGACATCAAGGCGGCCTGCGGCCAGCTCAAGGCTGCCCGGCAGAAGG
ATACAGCCGCGGCGGACGCTGATCCGAAAAATTGCGAATAA

Upstream 100 bases:

>100_bases
CCATCGCAAACAGCCCTGCCCTTTATCCTCCACCTTCCACTCGTCGCAGGTTCTCCCTCAACAAATCCCCTTGTGCTCTC
CGGCGCCTCCCGGTAAACAG

Downstream 100 bases:

>100_bases
AAAGCGTGCCCGCCCCGGACTGCGCAACGGACACGCGCACAACTCTGCGCAGGCCTGGGGCGGTTTTTCCTGCTGACAAA
AAAACGGCCCGGCCTTATAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSGPFAQGAAATVTQDEQSGRTMTNLLNLTLPELEAWTQSELGEPKFRAMQLWQWIWQRMARDFDTMTNISRPCRELLAA
KACIVWPEVSAVEESRDGTTKFLLRLEDGAQIETVLIPSDSREGVRRWTQCLSCQVGCTMGCTFCSTGQMGFERNMTMGE
ILGQILVAREHLGDTRLHWPILRNLVFMGMGEPLLNLKEVMRSLESLNNDKGLGFSPRRITVSTCGIEKGLKELGESGLA
FLAVSLHAPNQAVRERIMPKAARWRLNDLMAALKSYPLKTREHITFEYLMLGGVNDSLEHARELAPLVSAVKGKLNIIVY
NAAEGSPYQAPSEERILAFEKYLWSKDITAIIRKSKGQDIKAACGQLKAARQKDTAAADADPKNCE

Sequences:

>Translated_386_residues
MSGPFAQGAAATVTQDEQSGRTMTNLLNLTLPELEAWTQSELGEPKFRAMQLWQWIWQRMARDFDTMTNISRPCRELLAA
KACIVWPEVSAVEESRDGTTKFLLRLEDGAQIETVLIPSDSREGVRRWTQCLSCQVGCTMGCTFCSTGQMGFERNMTMGE
ILGQILVAREHLGDTRLHWPILRNLVFMGMGEPLLNLKEVMRSLESLNNDKGLGFSPRRITVSTCGIEKGLKELGESGLA
FLAVSLHAPNQAVRERIMPKAARWRLNDLMAALKSYPLKTREHITFEYLMLGGVNDSLEHARELAPLVSAVKGKLNIIVY
NAAEGSPYQAPSEERILAFEKYLWSKDITAIIRKSKGQDIKAACGQLKAARQKDTAAADADPKNCE
>Mature_385_residues
SGPFAQGAAATVTQDEQSGRTMTNLLNLTLPELEAWTQSELGEPKFRAMQLWQWIWQRMARDFDTMTNISRPCRELLAAK
ACIVWPEVSAVEESRDGTTKFLLRLEDGAQIETVLIPSDSREGVRRWTQCLSCQVGCTMGCTFCSTGQMGFERNMTMGEI
LGQILVAREHLGDTRLHWPILRNLVFMGMGEPLLNLKEVMRSLESLNNDKGLGFSPRRITVSTCGIEKGLKELGESGLAF
LAVSLHAPNQAVRERIMPKAARWRLNDLMAALKSYPLKTREHITFEYLMLGGVNDSLEHARELAPLVSAVKGKLNIIVYN
AAEGSPYQAPSEERILAFEKYLWSKDITAIIRKSKGQDIKAACGQLKAARQKDTAAADADPKNCE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=348, Percent_Identity=41.6666666666667, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43023; Mature: 42892

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPFAQGAAATVTQDEQSGRTMTNLLNLTLPELEAWTQSELGEPKFRAMQLWQWIWQRM
CCCCCCCCCCEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ARDFDTMTNISRPCRELLAAKACIVWPEVSAVEESRDGTTKFLLRLEDGAQIETVLIPSD
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCCEEEEEEECCCCEEEEEEECCC
SREGVRRWTQCLSCQVGCTMGCTFCSTGQMGFERNMTMGEILGQILVAREHLGDTRLHWP
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
ILRNLVFMGMGEPLLNLKEVMRSLESLNNDKGLGFSPRRITVSTCGIEKGLKELGESGLA
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEE
FLAVSLHAPNQAVRERIMPKAARWRLNDLMAALKSYPLKTREHITFEYLMLGGVNDSLEH
EEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCEEEEEEECCCCHHHHH
ARELAPLVSAVKGKLNIIVYNAAEGSPYQAPSEERILAFEKYLWSKDITAIIRKSKGQDI
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KAACGQLKAARQKDTAAADADPKNCE
HHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SGPFAQGAAATVTQDEQSGRTMTNLLNLTLPELEAWTQSELGEPKFRAMQLWQWIWQRM
CCCCCCCCCEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ARDFDTMTNISRPCRELLAAKACIVWPEVSAVEESRDGTTKFLLRLEDGAQIETVLIPSD
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCCEEEEEEECCCCEEEEEEECCC
SREGVRRWTQCLSCQVGCTMGCTFCSTGQMGFERNMTMGEILGQILVAREHLGDTRLHWP
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
ILRNLVFMGMGEPLLNLKEVMRSLESLNNDKGLGFSPRRITVSTCGIEKGLKELGESGLA
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEE
FLAVSLHAPNQAVRERIMPKAARWRLNDLMAALKSYPLKTREHITFEYLMLGGVNDSLEH
EEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCEEEEEEECCCCHHHHH
ARELAPLVSAVKGKLNIIVYNAAEGSPYQAPSEERILAFEKYLWSKDITAIIRKSKGQDI
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KAACGQLKAARQKDTAAADADPKNCE
HHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA