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The map label for this gene is tyrS [H]

Identifier: 220903632

GI number: 220903632

Start: 430796

End: 431992

Strand: Reverse

Name: tyrS [H]

Synonym: Ddes_0352

Alternate gene names: 220903632

Gene position: 431992-430796 (Counterclockwise)

Preceding gene: 220903633

Following gene: 220903630

Centisome position: 15.03

GC content: 57.98

Gene sequence:

>1197_bases
ATGACCGACATTGACCGCCAAATGGCTACCATCAAGCGCGGCGTGGCCGAACTGATCGACGAAAACGAACTGCGCAAGAA
GATCCTGCGCGGCGCTCCCCTGCGCATCAAAGTGGGCTTTGACCCCACCGCCCCCGACCTGCATCTGGGACATACGGTAG
TCATGCACAAGATGCGTCACTTTCAGGAACTGGGCCACCATGTAGTCTTTCTTATCGGCGATTTCACCGGGCGCATCGGT
GACCCTTCGGGCCGTTCTGAAACCCGCCCCCCGCTCACTGAAGAGCAGGTGCTCGCCAATGCCGAGACCTACAAAAAACA
GGTCTTCAAAATCCTTGACCCCGCCAAGACCGAAGTGGCCTTCAACTCCGCATGGCTCGGCAAGCTCGACGCCACCGGCT
TTATCAAGCTTGCATCCAGCTATACCGTCGCCCGTATGATGGAGCGCGACGATTTCGAAAAGCGCTTCCGCGAGCAACGG
CCCATTTCGATCCACGAATTTCTCTATCCCCTCTGCCAGGGGTATGACTCCGTATGCCTCAAGACCGATGTGGAGATGGG
CGGCACCGACCAGAAGTTCAACCTGCTGGTCGGCCGCAATCTTCAGGCCCACTACGGCATCGAAAGCCAGTGCATTCTCA
CCATGCCCCTGCTTGAAGGCACTGACGGCGTACGCAAGATGTCAAAATCTTACGGCAACTATATCGGCATTGACGAAGCG
CCTTCCGAGATTTTCGGCAAGATCATGGCCATTTCCGACGACCTCATGTGGCGCTACTACGAACTGCTTTCAACCAAAAG
CCTGGAAGATATCGCCACCCTCAAGACGGATGTGGCCACCGGCACGGTGCACCCCAAAGCCGCCAAGGAAACGCTGGCCC
ACGAAATGGTCAGCCGTTACCACAGCCGGAAAGACGCCGACGAAGCCCAGCAGGGCTTTAATGCGGTCTTTGCGGATGGC
GGCGTACCTGACGAAATGCCCGAGCACCAGTGCGCGCACGGCGAAGACAGCACCCCTCCGGTCTTTCTTGAAGCCGCGGG
CCTGGTCAAAAGCCGCGGTGAGGCCAAACGCCTCATGAAGGAAGGAGCACTCTCCATCGACGGGCAACGCTGCGACGACG
CCATAACGCCCCTGTCCGCCGGGGAATATGTGATCAAACTGGGCAAAAAGCGCTTTTTGAAGCTGCTGGTCAGCTAA

Upstream 100 bases:

>100_bases
TACTGTCACCTGTCCAAGTCAGGCACAGAACATCGGTTCTGTGCCGAATTTTTTACCGCAACAGATTTATTACCGCAGCC
GGTACATGAGGATAGGGCAA

Downstream 100 bases:

>100_bases
CACAGTTTACACATGCCTGAGCATATGCCGCCCTGCATTTGTAAACTCACTCCGGCGTCCGGCAGCGCGTAAGCGGCATT
GAACACCCCTGTGGCGGATG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTDIDRQMATIKRGVAELIDENELRKKILRGAPLRIKVGFDPTAPDLHLGHTVVMHKMRHFQELGHHVVFLIGDFTGRIG
DPSGRSETRPPLTEEQVLANAETYKKQVFKILDPAKTEVAFNSAWLGKLDATGFIKLASSYTVARMMERDDFEKRFREQR
PISIHEFLYPLCQGYDSVCLKTDVEMGGTDQKFNLLVGRNLQAHYGIESQCILTMPLLEGTDGVRKMSKSYGNYIGIDEA
PSEIFGKIMAISDDLMWRYYELLSTKSLEDIATLKTDVATGTVHPKAAKETLAHEMVSRYHSRKDADEAQQGFNAVFADG
GVPDEMPEHQCAHGEDSTPPVFLEAAGLVKSRGEAKRLMKEGALSIDGQRCDDAITPLSAGEYVIKLGKKRFLKLLVS

Sequences:

>Translated_398_residues
MTDIDRQMATIKRGVAELIDENELRKKILRGAPLRIKVGFDPTAPDLHLGHTVVMHKMRHFQELGHHVVFLIGDFTGRIG
DPSGRSETRPPLTEEQVLANAETYKKQVFKILDPAKTEVAFNSAWLGKLDATGFIKLASSYTVARMMERDDFEKRFREQR
PISIHEFLYPLCQGYDSVCLKTDVEMGGTDQKFNLLVGRNLQAHYGIESQCILTMPLLEGTDGVRKMSKSYGNYIGIDEA
PSEIFGKIMAISDDLMWRYYELLSTKSLEDIATLKTDVATGTVHPKAAKETLAHEMVSRYHSRKDADEAQQGFNAVFADG
GVPDEMPEHQCAHGEDSTPPVFLEAAGLVKSRGEAKRLMKEGALSIDGQRCDDAITPLSAGEYVIKLGKKRFLKLLVS
>Mature_397_residues
TDIDRQMATIKRGVAELIDENELRKKILRGAPLRIKVGFDPTAPDLHLGHTVVMHKMRHFQELGHHVVFLIGDFTGRIGD
PSGRSETRPPLTEEQVLANAETYKKQVFKILDPAKTEVAFNSAWLGKLDATGFIKLASSYTVARMMERDDFEKRFREQRP
ISIHEFLYPLCQGYDSVCLKTDVEMGGTDQKFNLLVGRNLQAHYGIESQCILTMPLLEGTDGVRKMSKSYGNYIGIDEAP
SEIFGKIMAISDDLMWRYYELLSTKSLEDIATLKTDVATGTVHPKAAKETLAHEMVSRYHSRKDADEAQQGFNAVFADGG
VPDEMPEHQCAHGEDSTPPVFLEAAGLVKSRGEAKRLMKEGALSIDGQRCDDAITPLSAGEYVIKLGKKRFLKLLVS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=26.0651629072682, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787925, Length=387, Percent_Identity=29.4573643410853, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25145802, Length=370, Percent_Identity=25.1351351351351, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=257, Percent_Identity=27.6264591439689, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130383, Length=390, Percent_Identity=25.1282051282051, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44449; Mature: 44318

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIDRQMATIKRGVAELIDENELRKKILRGAPLRIKVGFDPTAPDLHLGHTVVMHKMRH
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQELGHHVVFLIGDFTGRIGDPSGRSETRPPLTEEQVLANAETYKKQVFKILDPAKTEVA
HHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHE
FNSAWLGKLDATGFIKLASSYTVARMMERDDFEKRFREQRPISIHEFLYPLCQGYDSVCL
ECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
KTDVEMGGTDQKFNLLVGRNLQAHYGIESQCILTMPLLEGTDGVRKMSKSYGNYIGIDEA
EECCCCCCCCCEEEEEECCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
PSEIFGKIMAISDDLMWRYYELLSTKSLEDIATLKTDVATGTVHPKAAKETLAHEMVSRY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HSRKDADEAQQGFNAVFADGGVPDEMPEHQCAHGEDSTPPVFLEAAGLVKSRGEAKRLMK
HCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCHHHHHHHH
EGALSIDGQRCDDAITPLSAGEYVIKLGKKRFLKLLVS
HCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDIDRQMATIKRGVAELIDENELRKKILRGAPLRIKVGFDPTAPDLHLGHTVVMHKMRH
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQELGHHVVFLIGDFTGRIGDPSGRSETRPPLTEEQVLANAETYKKQVFKILDPAKTEVA
HHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHE
FNSAWLGKLDATGFIKLASSYTVARMMERDDFEKRFREQRPISIHEFLYPLCQGYDSVCL
ECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
KTDVEMGGTDQKFNLLVGRNLQAHYGIESQCILTMPLLEGTDGVRKMSKSYGNYIGIDEA
EECCCCCCCCCEEEEEECCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
PSEIFGKIMAISDDLMWRYYELLSTKSLEDIATLKTDVATGTVHPKAAKETLAHEMVSRY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HSRKDADEAQQGFNAVFADGGVPDEMPEHQCAHGEDSTPPVFLEAAGLVKSRGEAKRLMK
HCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCHHHHHHHH
EGALSIDGQRCDDAITPLSAGEYVIKLGKKRFLKLLVS
HCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA