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The map label for this gene is paaK [H]

Identifier: 220903600

GI number: 220903600

Start: 392001

End: 393266

Strand: Direct

Name: paaK [H]

Synonym: Ddes_0319

Alternate gene names: 220903600

Gene position: 392001-393266 (Clockwise)

Preceding gene: 220903599

Following gene: 220903601

Centisome position: 13.64

GC content: 56.79

Gene sequence:

>1266_bases
ATGACCCGTAAAGACCGCACAGAAGGCATATACAGCCGCCGCGAAGTTCTGGATGAAAGCGAGCGCCGCCAGTACTGCCT
TATCCAGCTCAAGGACTTGCTCTCCTATGCGTACCGCTATTCGGAAGACGTGAAGAAACGCTTCGACCGTGCGCAGTTCA
ATGTGGAGAAATTCAAGACCCTTGCTGACATCAAGCATATTCCCATTCTCAAAAAGAAAGAGCTTATCTTTCTTCAGTCC
ATGGGGCCGCGTCTGGGCGGACTGCTGACCAAGGATATCGGCGAACTCAAACGCATTTTTCTTTCCCCCGGCCCCATTTT
TGACCCCGAAGACCGTGGCGAAGATTACTGGGGCTATACTGAAGCCTTTTATTCTGTGGGCTTTCGCCCCGGCGACGCAG
TGCAGAATACCTTCAACTACCAGTTGACGCCTGCGGGCCTCATGTTTGAAGAGCCTTTGCGCAATCTGGGCTGTGCGGTC
ATTCCCGCAGGGCCGACCGATGCCGCCACTCAGCTGGACATCATGCAGAAGCTGCGCGTGTCCGGCTATGTGGGGACGCC
GAGCTTTTTGATGCACCTGGCGCAAAAGGCCGAAGAAAAAGGCCTCAATCTGCGCAAGGATCTCTTTCTGGAGGTGGCTT
TTGTCACCGGCGAGCGTCTTTCCGAAAAAATGCGCTCGCAGATGGAAAAGAAGTACGACCTTGTCATGCGGCAAGGCTAC
GGCACGGCTGACGTGGGCTGCATCGGCTATGAATGCTTCCACAAGACCGGACTGCATGTGGCCAACCGCTGCTTTGTGGA
AATATGCCACCCTGATACGGGCATTCCGCTCAAGGACGGCGAAGTGGGCGAAATTGTGGTCACGGCATTCAACAAGACCT
ATCCGCTCATCCGTCTGGCTACGGGCGACCTTTCGTACATCGATCGCAGCCCCTGCGCCTGCGGCCGCACCAGCCCGCGC
CTGGGCAGCATTGTAGGCCGCGTGGACACCACAGCCCGTATCATGGGCATGTTTGTCTACCCGCATCAGGTTGAGCAGGT
CATGAGCCGCTTTGAAGAAATCAAGCGTTGGCAGATCGAAGTCACCAACCCCGGCGGCATCGACGAAATGACCCTTTTTG
TGGAAACAAGCGCCTTCAAGCGCGAAGAGGAACTGCTGCACCAGTTCCGCGAAAAGATCAAACTGCGCCCCGAACTGCGC
GTGCTGGCTCCCGGCAGCCTGCCCCCGCAGATCCGGCCCATCGAAGACAAGCGCCACTGGGACTAG

Upstream 100 bases:

>100_bases
AATATTTTGATATTATTAAACTTAATAAATAGTGCTTGCACTTTGCTGTGGGAGTGGCTATTTTGACCCTTCAATGTAAA
TCAGGCCATGTGGAGTAGGT

Downstream 100 bases:

>100_bases
CACGCTTTGCCCTTGAGAATGCAGCCCTTCTCAAGGAGAGCACAAAGCTGTTCCGGCGTTTGACAGCAGTTTACGTTTCT
GGCAGCGGGCAATGGCACAG

Product: phenylacetate-coenzyme A ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTRKDRTEGIYSRREVLDESERRQYCLIQLKDLLSYAYRYSEDVKKRFDRAQFNVEKFKTLADIKHIPILKKKELIFLQS
MGPRLGGLLTKDIGELKRIFLSPGPIFDPEDRGEDYWGYTEAFYSVGFRPGDAVQNTFNYQLTPAGLMFEEPLRNLGCAV
IPAGPTDAATQLDIMQKLRVSGYVGTPSFLMHLAQKAEEKGLNLRKDLFLEVAFVTGERLSEKMRSQMEKKYDLVMRQGY
GTADVGCIGYECFHKTGLHVANRCFVEICHPDTGIPLKDGEVGEIVVTAFNKTYPLIRLATGDLSYIDRSPCACGRTSPR
LGSIVGRVDTTARIMGMFVYPHQVEQVMSRFEEIKRWQIEVTNPGGIDEMTLFVETSAFKREEELLHQFREKIKLRPELR
VLAPGSLPPQIRPIEDKRHWD

Sequences:

>Translated_421_residues
MTRKDRTEGIYSRREVLDESERRQYCLIQLKDLLSYAYRYSEDVKKRFDRAQFNVEKFKTLADIKHIPILKKKELIFLQS
MGPRLGGLLTKDIGELKRIFLSPGPIFDPEDRGEDYWGYTEAFYSVGFRPGDAVQNTFNYQLTPAGLMFEEPLRNLGCAV
IPAGPTDAATQLDIMQKLRVSGYVGTPSFLMHLAQKAEEKGLNLRKDLFLEVAFVTGERLSEKMRSQMEKKYDLVMRQGY
GTADVGCIGYECFHKTGLHVANRCFVEICHPDTGIPLKDGEVGEIVVTAFNKTYPLIRLATGDLSYIDRSPCACGRTSPR
LGSIVGRVDTTARIMGMFVYPHQVEQVMSRFEEIKRWQIEVTNPGGIDEMTLFVETSAFKREEELLHQFREKIKLRPELR
VLAPGSLPPQIRPIEDKRHWD
>Mature_420_residues
TRKDRTEGIYSRREVLDESERRQYCLIQLKDLLSYAYRYSEDVKKRFDRAQFNVEKFKTLADIKHIPILKKKELIFLQSM
GPRLGGLLTKDIGELKRIFLSPGPIFDPEDRGEDYWGYTEAFYSVGFRPGDAVQNTFNYQLTPAGLMFEEPLRNLGCAVI
PAGPTDAATQLDIMQKLRVSGYVGTPSFLMHLAQKAEEKGLNLRKDLFLEVAFVTGERLSEKMRSQMEKKYDLVMRQGYG
TADVGCIGYECFHKTGLHVANRCFVEICHPDTGIPLKDGEVGEIVVTAFNKTYPLIRLATGDLSYIDRSPCACGRTSPRL
GSIVGRVDTTARIMGMFVYPHQVEQVMSRFEEIKRWQIEVTNPGGIDEMTLFVETSAFKREEELLHQFREKIKLRPELRV
LAPGSLPPQIRPIEDKRHWD

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=403, Percent_Identity=26.0545905707196, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48239; Mature: 48108

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKDRTEGIYSRREVLDESERRQYCLIQLKDLLSYAYRYSEDVKKRFDRAQFNVEKFKT
CCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LADIKHIPILKKKELIFLQSMGPRLGGLLTKDIGELKRIFLSPGPIFDPEDRGEDYWGYT
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
EAFYSVGFRPGDAVQNTFNYQLTPAGLMFEEPLRNLGCAVIPAGPTDAATQLDIMQKLRV
HHHHHCCCCCCHHHHCCCCEEECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
SGYVGTPSFLMHLAQKAEEKGLNLRKDLFLEVAFVTGERLSEKMRSQMEKKYDLVMRQGY
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTADVGCIGYECFHKTGLHVANRCFVEICHPDTGIPLKDGEVGEIVVTAFNKTYPLIRLA
CCCCCHHHHHHHHHHHCHHHHCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
TGDLSYIDRSPCACGRTSPRLGSIVGRVDTTARIMGMFVYPHQVEQVMSRFEEIKRWQIE
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEE
VTNPGGIDEMTLFVETSAFKREEELLHQFREKIKLRPELRVLAPGSLPPQIRPIEDKRHW
ECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
D
C
>Mature Secondary Structure 
TRKDRTEGIYSRREVLDESERRQYCLIQLKDLLSYAYRYSEDVKKRFDRAQFNVEKFKT
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LADIKHIPILKKKELIFLQSMGPRLGGLLTKDIGELKRIFLSPGPIFDPEDRGEDYWGYT
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
EAFYSVGFRPGDAVQNTFNYQLTPAGLMFEEPLRNLGCAVIPAGPTDAATQLDIMQKLRV
HHHHHCCCCCCHHHHCCCCEEECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
SGYVGTPSFLMHLAQKAEEKGLNLRKDLFLEVAFVTGERLSEKMRSQMEKKYDLVMRQGY
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTADVGCIGYECFHKTGLHVANRCFVEICHPDTGIPLKDGEVGEIVVTAFNKTYPLIRLA
CCCCCHHHHHHHHHHHCHHHHCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
TGDLSYIDRSPCACGRTSPRLGSIVGRVDTTARIMGMFVYPHQVEQVMSRFEEIKRWQIE
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEE
VTNPGGIDEMTLFVETSAFKREEELLHQFREKIKLRPELRVLAPGSLPPQIRPIEDKRHW
ECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]