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The map label for this gene is clcA [H]

Identifier: 220903599

GI number: 220903599

Start: 390349

End: 391884

Strand: Direct

Name: clcA [H]

Synonym: Ddes_0318

Alternate gene names: 220903599

Gene position: 390349-391884 (Clockwise)

Preceding gene: 220903596

Following gene: 220903600

Centisome position: 13.58

GC content: 60.16

Gene sequence:

>1536_bases
ATGCTTTTTTCCTTCCCCGCGGCGGATACGTGGTTTTCGGCTGGCTCCATCAATGTGCGGCGCGTTTGTCCGGCCGCAGA
TTCTGCAAAAAATGCCTCGAATAAGGGTATGTTAGTGAAAACAGGCAATCCTTTTCTCCAGATCAAGCACGACGTTACAG
AAATGGGTGTTTTCGGTCTGGGCAAGATTATTGTGCAGGCCCTGCTTGTGGGCAGCGCCACCGGGGCTGTGGTTGGCCTG
TTCCGGCACCTGAATGACGCCATCACCCTCTGGCTTGTGGGGGCTGTGGGGCAGCGCGGTCTTGCAGATCACCTCATGGT
GCTGGCCGTTTTTGGCGGTCTTTTGCTTTTGGCCGTCATTTCGGTGCTGCTTTTGCGCGCGGAGCCGCTCATCAGCGGCA
GCGGCATCCCCCAGGTGGAGTTGATGGTCAAGGGCAAGTTGCGCATGAACTGGCTGCGCGTACTGCTGTGCAAGTTCGCC
GGTGCGCTGGCCGCCCTTACGGGCGGCCTTTCTGTGGGACGCGAGGGGCCGTGCATCATGATGGGAGCCTCTCTGGGCGT
TGGAGTGGGGACCCTGTGGAAGGACAGCACCGCTGCCAATAACCCCCGTTACCTCGTGGGGGGCAGTGCCGCCGGTCTTG
CCGCCGCCTTTGGCGCGCCGCTGGCAGGCATGTGCTTTGCCTTTGAGGAAATGAAAACCCCCCTCAGGGCGCCCATGGTC
ATAACCTGCGCCATCACGGCCTTTGCGGCCATCTATGTGATGCAGGGCATTTTCGGCTTCGGGCTGGTCTTTCCCTTTGG
GGTGCACTCCATGCTGCCGTGGCGCGAATGGTGGCTCGTGCCCGTTGCCGGTGTGGTTATGGGCGGGCTTGGCGTACTGT
ATAATACCGTGCTCATACGCATGACCCTCTGGGCGGATAAGACAAGGTTGCTGCCCTTGCATTTACGGGTAGTCATACCC
TTTATGTGCGCCGGTCTGTTTTTGTATTTTTATCCCACAGTGCTGGCTGGCTTCGGCATAGGAGCGCTGGATCTGGCGCG
CCTGTCCATCCCGCTGACCGCGCTTTTGCTGCTGCTGGGCGTCAAGATGCTTTTTTCCTGTGCCAGTTTTGCCACGGGCG
TGGCAGGGGGGCTGCTCATGCCCATCCTGTTTATGGGCGGGATGGCCGGGGCCTGTCTTGCATCCACCATGCTGCTTTTT
GACATGGTAGCGCCGGACAGGGCCGCCACCATACTCATCCTGTGCATGGCAGGGCTGTTCGGTTCATCCGTGCGCGCCCC
CCTTACAGGCGCTTTTCTTATGCTTGAAATGACAGACTCTTACCATAACATGGTCTTTGTTATCCTTGCGGCGTACATTG
CGGCCTTTGTGGCCGACAAGCTCGGCAACGAGCCTGTGTATGACAGCCTGCGCCGCCGCTGTGTCATCCAGGGCAGGATC
AGGATGCCCCGGCGCAAGCCCCGCCGCCCGCAAGGCCCGCAAGGGGGGGCAGGGGGCGCAATGGCGAAGCGGGCTTTTTC
GCAACCTGGCACATAG

Upstream 100 bases:

>100_bases
ACCATGCGTGTTCGGCCTGCTGTTATTCTGCCGCCGGTCTGTTGTCGGCCTGTCATTGTTTGGCCGTCGGCGTGTCGCGA
GTCCGGTCCCGGCAGGGCGG

Downstream 100 bases:

>100_bases
CACAAAAGTGTGCTATAATATTTTGATATTATTAAACTTAATAAATAGTGCTTGCACTTTGCTGTGGGAGTGGCTATTTT
GACCCTTCAATGTAAATCAG

Product: Chloride channel core

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MLFSFPAADTWFSAGSINVRRVCPAADSAKNASNKGMLVKTGNPFLQIKHDVTEMGVFGLGKIIVQALLVGSATGAVVGL
FRHLNDAITLWLVGAVGQRGLADHLMVLAVFGGLLLLAVISVLLLRAEPLISGSGIPQVELMVKGKLRMNWLRVLLCKFA
GALAALTGGLSVGREGPCIMMGASLGVGVGTLWKDSTAANNPRYLVGGSAAGLAAAFGAPLAGMCFAFEEMKTPLRAPMV
ITCAITAFAAIYVMQGIFGFGLVFPFGVHSMLPWREWWLVPVAGVVMGGLGVLYNTVLIRMTLWADKTRLLPLHLRVVIP
FMCAGLFLYFYPTVLAGFGIGALDLARLSIPLTALLLLLGVKMLFSCASFATGVAGGLLMPILFMGGMAGACLASTMLLF
DMVAPDRAATILILCMAGLFGSSVRAPLTGAFLMLEMTDSYHNMVFVILAAYIAAFVADKLGNEPVYDSLRRRCVIQGRI
RMPRRKPRRPQGPQGGAGGAMAKRAFSQPGT

Sequences:

>Translated_511_residues
MLFSFPAADTWFSAGSINVRRVCPAADSAKNASNKGMLVKTGNPFLQIKHDVTEMGVFGLGKIIVQALLVGSATGAVVGL
FRHLNDAITLWLVGAVGQRGLADHLMVLAVFGGLLLLAVISVLLLRAEPLISGSGIPQVELMVKGKLRMNWLRVLLCKFA
GALAALTGGLSVGREGPCIMMGASLGVGVGTLWKDSTAANNPRYLVGGSAAGLAAAFGAPLAGMCFAFEEMKTPLRAPMV
ITCAITAFAAIYVMQGIFGFGLVFPFGVHSMLPWREWWLVPVAGVVMGGLGVLYNTVLIRMTLWADKTRLLPLHLRVVIP
FMCAGLFLYFYPTVLAGFGIGALDLARLSIPLTALLLLLGVKMLFSCASFATGVAGGLLMPILFMGGMAGACLASTMLLF
DMVAPDRAATILILCMAGLFGSSVRAPLTGAFLMLEMTDSYHNMVFVILAAYIAAFVADKLGNEPVYDSLRRRCVIQGRI
RMPRRKPRRPQGPQGGAGGAMAKRAFSQPGT
>Mature_511_residues
MLFSFPAADTWFSAGSINVRRVCPAADSAKNASNKGMLVKTGNPFLQIKHDVTEMGVFGLGKIIVQALLVGSATGAVVGL
FRHLNDAITLWLVGAVGQRGLADHLMVLAVFGGLLLLAVISVLLLRAEPLISGSGIPQVELMVKGKLRMNWLRVLLCKFA
GALAALTGGLSVGREGPCIMMGASLGVGVGTLWKDSTAANNPRYLVGGSAAGLAAAFGAPLAGMCFAFEEMKTPLRAPMV
ITCAITAFAAIYVMQGIFGFGLVFPFGVHSMLPWREWWLVPVAGVVMGGLGVLYNTVLIRMTLWADKTRLLPLHLRVVIP
FMCAGLFLYFYPTVLAGFGIGALDLARLSIPLTALLLLLGVKMLFSCASFATGVAGGLLMPILFMGGMAGACLASTMLLF
DMVAPDRAATILILCMAGLFGSSVRAPLTGAFLMLEMTDSYHNMVFVILAAYIAAFVADKLGNEPVYDSLRRRCVIQGRI
RMPRRKPRRPQGPQGGAGGAMAKRAFSQPGT

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI153252026, Length=420, Percent_Identity=24.7619047619048, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1786350, Length=437, Percent_Identity=29.0617848970252, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17532615, Length=403, Percent_Identity=25.0620347394541, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24665008, Length=422, Percent_Identity=23.2227488151659, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 54049; Mature: 54049

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSFPAADTWFSAGSINVRRVCPAADSAKNASNKGMLVKTGNPFLQIKHDVTEMGVFGL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHH
GKIIVQALLVGSATGAVVGLFRHLNDAITLWLVGAVGQRGLADHLMVLAVFGGLLLLAVI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SVLLLRAEPLISGSGIPQVELMVKGKLRMNWLRVLLCKFAGALAALTGGLSVGREGPCIM
HHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
MGASLGVGVGTLWKDSTAANNPRYLVGGSAAGLAAAFGAPLAGMCFAFEEMKTPLRAPMV
EECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
ITCAITAFAAIYVMQGIFGFGLVFPFGVHSMLPWREWWLVPVAGVVMGGLGVLYNTVLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLWADKTRLLPLHLRVVIPFMCAGLFLYFYPTVLAGFGIGALDLARLSIPLTALLLLLG
HHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VKMLFSCASFATGVAGGLLMPILFMGGMAGACLASTMLLFDMVAPDRAATILILCMAGLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GSSVRAPLTGAFLMLEMTDSYHNMVFVILAAYIAAFVADKLGNEPVYDSLRRRCVIQGRI
CCCCCCCHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEHHCCC
RMPRRKPRRPQGPQGGAGGAMAKRAFSQPGT
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLFSFPAADTWFSAGSINVRRVCPAADSAKNASNKGMLVKTGNPFLQIKHDVTEMGVFGL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHH
GKIIVQALLVGSATGAVVGLFRHLNDAITLWLVGAVGQRGLADHLMVLAVFGGLLLLAVI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SVLLLRAEPLISGSGIPQVELMVKGKLRMNWLRVLLCKFAGALAALTGGLSVGREGPCIM
HHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
MGASLGVGVGTLWKDSTAANNPRYLVGGSAAGLAAAFGAPLAGMCFAFEEMKTPLRAPMV
EECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
ITCAITAFAAIYVMQGIFGFGLVFPFGVHSMLPWREWWLVPVAGVVMGGLGVLYNTVLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLWADKTRLLPLHLRVVIPFMCAGLFLYFYPTVLAGFGIGALDLARLSIPLTALLLLLG
HHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VKMLFSCASFATGVAGGLLMPILFMGGMAGACLASTMLLFDMVAPDRAATILILCMAGLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GSSVRAPLTGAFLMLEMTDSYHNMVFVILAAYIAAFVADKLGNEPVYDSLRRRCVIQGRI
CCCCCCCHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEHHCCC
RMPRRKPRRPQGPQGGAGGAMAKRAFSQPGT
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA