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The map label for this gene is dacF [H]

Identifier: 220903565

GI number: 220903565

Start: 337388

End: 338524

Strand: Reverse

Name: dacF [H]

Synonym: Ddes_0284

Alternate gene names: 220903565

Gene position: 338524-337388 (Counterclockwise)

Preceding gene: 220903566

Following gene: 220903559

Centisome position: 11.78

GC content: 62.27

Gene sequence:

>1137_bases
ATGCCCAGCCGTTACCGCCACAAGAGGTTCAGCTTCCTCAATGTGTTTGCGTATCCTTTCCTGTTTTTGCTGCTGGCCTG
CCTGCTGCGTCCCGGCCCCGGACAGGCCGCCCCCCCCATTCCGGAACACGGTTTTCTGGAAACAAGTTCCGCCATTCTGT
ACGATCTGGACCACGATGCCATTCTTTTTGAGCAAAACGCCGACCAGCGCATTGCCCCGGCATCACTGACCAAGGTCCTT
TCAATGTTTCTGGCCCTGGACAGCGTGGATCAGGGCAAGCTGAGCCTGGACAATACCGTAACGGTCAGCCGTGCCGCCGC
AGGAACCGGCGGTTCACGCATGGGCCTGCGGGAAAATGACGTGGTGAGCCTTGAACAGCTGCTCATGGGCATGGCTGTGT
CTTCGGGCAATGATGCCAGCATGGCTGTGGCGGAATGTGTGGGCGGTTCGACTCCGGCTTTTGTAGCCATGATGAATGCC
AAAGCGCAGGCCCTGGGCATGCGCGACAGCCAGTTCCGCAATCCCCACGGGTTGCCCGCCAAAGGCCAGTACACCACGGC
CCGCGACATGCTCACCCTGGCACGGGCCTATCTGCAGGCCCATCCCCAGGCCCTGCGTTTTCACAACACCCACACCATCA
GCCATAAAGGGCGCGTCACCTGGAACAAGAACCCCCTGCTGGGCCAGTACCCCGGCGCGGACGGGCTTAAAACAGGCTGG
GTCAACGCCTCGGGCTACAATCTTATCTTCACGGCCAGCCAGGGGCCCAGACGCCTGCTGGCCGTCATTCTGGGCGCGCC
CGACTCGCGCACGCGCGGGGTTGAGGCGTTTCGCCTGCTGGATGCCGGATTTCAGGTATGCGGCAACAATGCCGCATCGG
TGGTGGCAGCCCTGGATTGTCTGCCCCCGGAACGCTATAACCCCGACATGCGCAAGACAGCCCGCGAGGCCCGGCGTATG
TATGCAGGCAGCGATGCCGCACCTCGCAAAAACGTGAGCAAGGCCAAGGCCGGCAAGGCCCCTCAGGCCAAGCAGACGGC
AAAAGCAAAGCCCAAAAAGAAAGAGGCCCACAAGGCCGCGCGACAAAAGCGCGATTCCGGCCAGGCAGCCAGATCCGGCT
CTGGACGGGCGGGCTAG

Upstream 100 bases:

>100_bases
AAATTCTAAACTTTTTCTTCACATGTCTGCCTGGCCCGCAATCCAGATCGGCCCGCGCGGTCTGCCCTGCCTTTCGCCAG
ACTTAGCCCAAGGATGCCCC

Downstream 100 bases:

>100_bases
GCGCCTTAAAGGACTGTGTATCTGCCAGGTCGCTGCACGGCTGGCGGCGGCAACTCCCCCGGGCTGGCAAAATATTGCTC
CCCCGCAGAGGCTGCCGCAC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPSRYRHKRFSFLNVFAYPFLFLLLACLLRPGPGQAAPPIPEHGFLETSSAILYDLDHDAILFEQNADQRIAPASLTKVL
SMFLALDSVDQGKLSLDNTVTVSRAAAGTGGSRMGLRENDVVSLEQLLMGMAVSSGNDASMAVAECVGGSTPAFVAMMNA
KAQALGMRDSQFRNPHGLPAKGQYTTARDMLTLARAYLQAHPQALRFHNTHTISHKGRVTWNKNPLLGQYPGADGLKTGW
VNASGYNLIFTASQGPRRLLAVILGAPDSRTRGVEAFRLLDAGFQVCGNNAASVVAALDCLPPERYNPDMRKTAREARRM
YAGSDAAPRKNVSKAKAGKAPQAKQTAKAKPKKKEAHKAARQKRDSGQAARSGSGRAG

Sequences:

>Translated_378_residues
MPSRYRHKRFSFLNVFAYPFLFLLLACLLRPGPGQAAPPIPEHGFLETSSAILYDLDHDAILFEQNADQRIAPASLTKVL
SMFLALDSVDQGKLSLDNTVTVSRAAAGTGGSRMGLRENDVVSLEQLLMGMAVSSGNDASMAVAECVGGSTPAFVAMMNA
KAQALGMRDSQFRNPHGLPAKGQYTTARDMLTLARAYLQAHPQALRFHNTHTISHKGRVTWNKNPLLGQYPGADGLKTGW
VNASGYNLIFTASQGPRRLLAVILGAPDSRTRGVEAFRLLDAGFQVCGNNAASVVAALDCLPPERYNPDMRKTAREARRM
YAGSDAAPRKNVSKAKAGKAPQAKQTAKAKPKKKEAHKAARQKRDSGQAARSGSGRAG
>Mature_377_residues
PSRYRHKRFSFLNVFAYPFLFLLLACLLRPGPGQAAPPIPEHGFLETSSAILYDLDHDAILFEQNADQRIAPASLTKVLS
MFLALDSVDQGKLSLDNTVTVSRAAAGTGGSRMGLRENDVVSLEQLLMGMAVSSGNDASMAVAECVGGSTPAFVAMMNAK
AQALGMRDSQFRNPHGLPAKGQYTTARDMLTLARAYLQAHPQALRFHNTHTISHKGRVTWNKNPLLGQYPGADGLKTGWV
NASGYNLIFTASQGPRRLLAVILGAPDSRTRGVEAFRLLDAGFQVCGNNAASVVAALDCLPPERYNPDMRKTAREARRMY
AGSDAAPRKNVSKAKAGKAPQAKQTAKAKPKKKEAHKAARQKRDSGQAARSGSGRAG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=279, Percent_Identity=34.4086021505376, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87082022, Length=256, Percent_Identity=36.71875, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786851, Length=255, Percent_Identity=34.9019607843137, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87082054, Length=253, Percent_Identity=26.4822134387352, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 40630; Mature: 40499

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRYRHKRFSFLNVFAYPFLFLLLACLLRPGPGQAAPPIPEHGFLETSSAILYDLDHDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCCE
ILFEQNADQRIAPASLTKVLSMFLALDSVDQGKLSLDNTVTVSRAAAGTGGSRMGLREND
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEHHCCCCCCCCCCCCCCC
VVSLEQLLMGMAVSSGNDASMAVAECVGGSTPAFVAMMNAKAQALGMRDSQFRNPHGLPA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHEEEHHHHHHHHCCCHHHCCCCCCCCC
KGQYTTARDMLTLARAYLQAHPQALRFHNTHTISHKGRVTWNKNPLLGQYPGADGLKTGW
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCE
VNASGYNLIFTASQGPRRLLAVILGAPDSRTRGVEAFRLLDAGFQVCGNNAASVVAALDC
ECCCCCEEEEECCCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LPPERYNPDMRKTAREARRMYAGSDAAPRKNVSKAKAGKAPQAKQTAKAKPKKKEAHKAA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHH
RQKRDSGQAARSGSGRAG
HHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
PSRYRHKRFSFLNVFAYPFLFLLLACLLRPGPGQAAPPIPEHGFLETSSAILYDLDHDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCCE
ILFEQNADQRIAPASLTKVLSMFLALDSVDQGKLSLDNTVTVSRAAAGTGGSRMGLREND
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEHHCCCCCCCCCCCCCCC
VVSLEQLLMGMAVSSGNDASMAVAECVGGSTPAFVAMMNAKAQALGMRDSQFRNPHGLPA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHEEEHHHHHHHHCCCHHHCCCCCCCCC
KGQYTTARDMLTLARAYLQAHPQALRFHNTHTISHKGRVTWNKNPLLGQYPGADGLKTGW
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCE
VNASGYNLIFTASQGPRRLLAVILGAPDSRTRGVEAFRLLDAGFQVCGNNAASVVAALDC
ECCCCCEEEEECCCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LPPERYNPDMRKTAREARRMYAGSDAAPRKNVSKAKAGKAPQAKQTAKAKPKKKEAHKAA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHH
RQKRDSGQAARSGSGRAG
HHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]