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The map label for this gene is ngoFVIIM [H]

Identifier: 220903552

GI number: 220903552

Start: 314426

End: 315358

Strand: Direct

Name: ngoFVIIM [H]

Synonym: Ddes_0271

Alternate gene names: 220903552

Gene position: 314426-315358 (Clockwise)

Preceding gene: 220903551

Following gene: 220903553

Centisome position: 10.94

GC content: 44.48

Gene sequence:

>933_bases
GTGCGAATTGTTTCTTTATTCAGTGGAGCCGGAGGCCTGGACTTAGGCCTGATTCAAGCGGGGCATGAAATTGTATGGGC
AAACGATTTTGATAAGGATTGCGTAGCTACCTATAAGAAAAATATCGGAAATCATGCGGTACTGGGCGATATAAAAAAAA
TTAACGCAAGCCAGATACCTCGAGGGGAGGTTGTTGTCGGGGGATTTCCGTGCCAAGGGTTTTCTCAGGCCAACCTTTTG
AGGTCTGGTAATGATGAACGTAATAGTTTGTATATTGAGTTTCTCAGGATTGTTTCTAGCCTCCAACCTCGCTATTTTTT
GGCTGAAAATGTAAGAGGGATTTTGAGTCTTGAAGGTGGTTCTGCTATAAAAAAGATTCTTGGCGACTTTAACAATGCGG
GCTACCGGTTAAAATACAAGCTTTTCAATGTTGCTGATTTCGGGGTTCCGCAGTCTCGTTTTAGGGTAATAATAGCGGGA
ACAAGAAAAGATATCCCTAAACGCCTTGACTATGAGTATCCCGCAGCTACTCACGCACAAGCACCGTCCCAAGGGAAACT
TCCCTGGGTTACGATAGGTGAAGCCCTGAAAGAAATTCCTGAACCAGGAGACGGGGTGGCGTTGCTGAATCACGACTATT
CCAAATATAAAATTACAAATCGAAACTTTACAGGACACCGGAAGACAGACCCAGGTAAGCCTTCTCCGACAATCCTTGCC
CGCGGTAATGGGCAAGGAGGGGTATGTGCTATACAACATCCGAACAATCATCGCCGGATGTCTGTTCGGGAACAGGCGAT
AATACAGTCTTTCCCTCTTGATTTTGAATTTGTTGGCAAGCTGAATTCTTGTTATAGGCAAGTTGGAAATGCTGTTCCTG
TAGAGTTTGCAAAATTTTTAGGAAAAGAGTTAACGCGGTTGGAGCAGAGATGA

Upstream 100 bases:

>100_bases
AGATAATCGTTAAAAATTTCATGGAAAATTAATTTACCCTGACGATTGCCTTACTCTCTTGATCTATTTACTATGCCCTT
TGGATAATTTGGAGAAAAGT

Downstream 100 bases:

>100_bases
AAGTAGTCTCTCTTTTTAGTGGAGCGGGAGGCCTTGATTTAGGTTTCAAAATGGCTGGTCACAGCATCATTTGGGCAAAT
GATGTTTATGAAGATGCTGT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoFVII; Cytosine-specific methyltransferase NgoFVII; M.NgoVII [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRIVSLFSGAGGLDLGLIQAGHEIVWANDFDKDCVATYKKNIGNHAVLGDIKKINASQIPRGEVVVGGFPCQGFSQANLL
RSGNDERNSLYIEFLRIVSSLQPRYFLAENVRGILSLEGGSAIKKILGDFNNAGYRLKYKLFNVADFGVPQSRFRVIIAG
TRKDIPKRLDYEYPAATHAQAPSQGKLPWVTIGEALKEIPEPGDGVALLNHDYSKYKITNRNFTGHRKTDPGKPSPTILA
RGNGQGGVCAIQHPNNHRRMSVREQAIIQSFPLDFEFVGKLNSCYRQVGNAVPVEFAKFLGKELTRLEQR

Sequences:

>Translated_310_residues
MRIVSLFSGAGGLDLGLIQAGHEIVWANDFDKDCVATYKKNIGNHAVLGDIKKINASQIPRGEVVVGGFPCQGFSQANLL
RSGNDERNSLYIEFLRIVSSLQPRYFLAENVRGILSLEGGSAIKKILGDFNNAGYRLKYKLFNVADFGVPQSRFRVIIAG
TRKDIPKRLDYEYPAATHAQAPSQGKLPWVTIGEALKEIPEPGDGVALLNHDYSKYKITNRNFTGHRKTDPGKPSPTILA
RGNGQGGVCAIQHPNNHRRMSVREQAIIQSFPLDFEFVGKLNSCYRQVGNAVPVEFAKFLGKELTRLEQR
>Mature_310_residues
MRIVSLFSGAGGLDLGLIQAGHEIVWANDFDKDCVATYKKNIGNHAVLGDIKKINASQIPRGEVVVGGFPCQGFSQANLL
RSGNDERNSLYIEFLRIVSSLQPRYFLAENVRGILSLEGGSAIKKILGDFNNAGYRLKYKLFNVADFGVPQSRFRVIIAG
TRKDIPKRLDYEYPAATHAQAPSQGKLPWVTIGEALKEIPEPGDGVALLNHDYSKYKITNRNFTGHRKTDPGKPSPTILA
RGNGQGGVCAIQHPNNHRRMSVREQAIIQSFPLDFEFVGKLNSCYRQVGNAVPVEFAKFLGKELTRLEQR

Specific function: This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=256, Percent_Identity=26.171875, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI195927037, Length=256, Percent_Identity=26.171875, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=26.4423076923077, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 34298; Mature: 34298

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVSLFSGAGGLDLGLIQAGHEIVWANDFDKDCVATYKKNIGNHAVLGDIKKINASQIP
CEEEEEECCCCCCEEHEEECCCEEEEECCCCHHHHHHHHHCCCCEEEECHHHHCCCCCCC
RGEVVVGGFPCQGFSQANLLRSGNDERNSLYIEFLRIVSSLQPRYFLAENVRGILSLEGG
CCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCEEEEECCC
SAIKKILGDFNNAGYRLKYKLFNVADFGVPQSRFRVIIAGTRKDIPKRLDYEYPAATHAQ
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHEEEEEECCCCCCHHHCCCCCCCCCCCC
APSQGKLPWVTIGEALKEIPEPGDGVALLNHDYSKYKITNRNFTGHRKTDPGKPSPTILA
CCCCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEE
RGNGQGGVCAIQHPNNHRRMSVREQAIIQSFPLDFEFVGKLNSCYRQVGNAVPVEFAKFL
ECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
GKELTRLEQR
HHHHHHHHCC
>Mature Secondary Structure
MRIVSLFSGAGGLDLGLIQAGHEIVWANDFDKDCVATYKKNIGNHAVLGDIKKINASQIP
CEEEEEECCCCCCEEHEEECCCEEEEECCCCHHHHHHHHHCCCCEEEECHHHHCCCCCCC
RGEVVVGGFPCQGFSQANLLRSGNDERNSLYIEFLRIVSSLQPRYFLAENVRGILSLEGG
CCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCEEEEECCC
SAIKKILGDFNNAGYRLKYKLFNVADFGVPQSRFRVIIAGTRKDIPKRLDYEYPAATHAQ
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHEEEEEECCCCCCHHHCCCCCCCCCCCC
APSQGKLPWVTIGEALKEIPEPGDGVALLNHDYSKYKITNRNFTGHRKTDPGKPSPTILA
CCCCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEE
RGNGQGGVCAIQHPNNHRRMSVREQAIIQSFPLDFEFVGKLNSCYRQVGNAVPVEFAKFL
ECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
GKELTRLEQR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]