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The map label for this gene is ngoFVIIM [H]

Identifier: 220903553

GI number: 220903553

Start: 315355

End: 316281

Strand: Direct

Name: ngoFVIIM [H]

Synonym: Ddes_0272

Alternate gene names: 220903553

Gene position: 315355-316281 (Clockwise)

Preceding gene: 220903552

Following gene: 220903554

Centisome position: 10.97

GC content: 45.95

Gene sequence:

>927_bases
ATGAAAGTAGTCTCTCTTTTTAGTGGAGCGGGAGGCCTTGATTTAGGTTTCAAAATGGCTGGTCACAGCATCATTTGGGC
AAATGATGTTTATGAAGATGCTGTGAAAACATACCGTATGAATATTGGAGATCATATCCTTTGCAAAGATATTTCTCAGG
TTACAACAGCGGAAGTTCCAGATAGTGATATCATCATAGGCGGGTTCCCTTGCCAAGGTTTTTCTGTCGCTAATATCAAA
CGCAATATTTCTGACGAGCGCAATACATTATATAAGCACTTATTAAGGGTCGTTTCCGCTAAACAGCCTAAATTTTTCCT
TGCGGAAAACGTCAAGGGGATACTTAGCTTAGGGAAGGGCCATGTCATCCAAATGATTTTGAATGATTTCGAAAATATTG
GGTACACCATGCAGCTCAAGCTGCTAAATGCCGCTGATTACGGAGTGCCGCAGACCCGCCAGCGTGTTTTTATTGCCGGT
GTGCGGAAAGACCTCGACGTTATTTTTACCTATCCTGATGCTACACATAGTTGCGAGGGAACTGGCGGGCTGAAAAAATG
GCGTAGTGTTGGCGAAGCACTTGCTGGCCTACCAGACCCAGACGAACCAAACGATATTCTCAATCATGACTATTCGAAAT
ATAAATTAAGGTTTAACGGCTATCTTGGGCATCGAGAAATTGATCCCGGCAAGCCCGCTCCGACTGTCACGGCGCGGGGG
GATGACAGGGGTGGGGTTGTGGTTCTTCATCACCCCAATAATCAACGCCGTATGTCTTGCCGCGAACTAGCCATTGTTCA
GGGCTTTCCTGTAGACTATGCTTTCTTTGGCAACCGTTCTTCTGTTTACCGGCAGGTAGGCAATGCCGTGCCTCCTCTTT
TAGCATACGCGATGGCGAAGCAATTTAACCAATATGCGTGCGAGTAA

Upstream 100 bases:

>100_bases
TTGTTGGCAAGCTGAATTCTTGTTATAGGCAAGTTGGAAATGCTGTTCCTGTAGAGTTTGCAAAATTTTTAGGAAAAGAG
TTAACGCGGTTGGAGCAGAG

Downstream 100 bases:

>100_bases
CGTTATGTTGCCTCAAAATTTTGTACCTCAAAAACCCTTTGCGGATTTCAAATGGAAATGGGCCAGCCTACAATGCACAG
AGAGCCTGAACGACCCAGTA

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoFVII; Cytosine-specific methyltransferase NgoFVII; M.NgoVII [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKVVSLFSGAGGLDLGFKMAGHSIIWANDVYEDAVKTYRMNIGDHILCKDISQVTTAEVPDSDIIIGGFPCQGFSVANIK
RNISDERNTLYKHLLRVVSAKQPKFFLAENVKGILSLGKGHVIQMILNDFENIGYTMQLKLLNAADYGVPQTRQRVFIAG
VRKDLDVIFTYPDATHSCEGTGGLKKWRSVGEALAGLPDPDEPNDILNHDYSKYKLRFNGYLGHREIDPGKPAPTVTARG
DDRGGVVVLHHPNNQRRMSCRELAIVQGFPVDYAFFGNRSSVYRQVGNAVPPLLAYAMAKQFNQYACE

Sequences:

>Translated_308_residues
MKVVSLFSGAGGLDLGFKMAGHSIIWANDVYEDAVKTYRMNIGDHILCKDISQVTTAEVPDSDIIIGGFPCQGFSVANIK
RNISDERNTLYKHLLRVVSAKQPKFFLAENVKGILSLGKGHVIQMILNDFENIGYTMQLKLLNAADYGVPQTRQRVFIAG
VRKDLDVIFTYPDATHSCEGTGGLKKWRSVGEALAGLPDPDEPNDILNHDYSKYKLRFNGYLGHREIDPGKPAPTVTARG
DDRGGVVVLHHPNNQRRMSCRELAIVQGFPVDYAFFGNRSSVYRQVGNAVPPLLAYAMAKQFNQYACE
>Mature_308_residues
MKVVSLFSGAGGLDLGFKMAGHSIIWANDVYEDAVKTYRMNIGDHILCKDISQVTTAEVPDSDIIIGGFPCQGFSVANIK
RNISDERNTLYKHLLRVVSAKQPKFFLAENVKGILSLGKGHVIQMILNDFENIGYTMQLKLLNAADYGVPQTRQRVFIAG
VRKDLDVIFTYPDATHSCEGTGGLKKWRSVGEALAGLPDPDEPNDILNHDYSKYKLRFNGYLGHREIDPGKPAPTVTARG
DDRGGVVVLHHPNNQRRMSCRELAIVQGFPVDYAFFGNRSSVYRQVGNAVPPLLAYAMAKQFNQYACE

Specific function: This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=177, Percent_Identity=32.2033898305085, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4503351, Length=177, Percent_Identity=32.2033898305085, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4758184, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788271, Length=361, Percent_Identity=25.7617728531856, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 34139; Mature: 34139

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVSLFSGAGGLDLGFKMAGHSIIWANDVYEDAVKTYRMNIGDHILCKDISQVTTAEVP
CCEEEEECCCCCCCCCEEECCCEEEEECHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
DSDIIIGGFPCQGFSVANIKRNISDERNTLYKHLLRVVSAKQPKFFLAENVKGILSLGKG
CCCEEEECCCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCC
HVIQMILNDFENIGYTMQLKLLNAADYGVPQTRQRVFIAGVRKDLDVIFTYPDATHSCEG
HHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHCEEEEEECCCCCEEEEECCCCCCCCCC
TGGLKKWRSVGEALAGLPDPDEPNDILNHDYSKYKLRFNGYLGHREIDPGKPAPTVTARG
CCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECC
DDRGGVVVLHHPNNQRRMSCRELAIVQGFPVDYAFFGNRSSVYRQVGNAVPPLLAYAMAK
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHH
QFNQYACE
HHHHHCCC
>Mature Secondary Structure
MKVVSLFSGAGGLDLGFKMAGHSIIWANDVYEDAVKTYRMNIGDHILCKDISQVTTAEVP
CCEEEEECCCCCCCCCEEECCCEEEEECHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
DSDIIIGGFPCQGFSVANIKRNISDERNTLYKHLLRVVSAKQPKFFLAENVKGILSLGKG
CCCEEEECCCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCC
HVIQMILNDFENIGYTMQLKLLNAADYGVPQTRQRVFIAGVRKDLDVIFTYPDATHSCEG
HHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHCEEEEEECCCCCEEEEECCCCCCCCCC
TGGLKKWRSVGEALAGLPDPDEPNDILNHDYSKYKLRFNGYLGHREIDPGKPAPTVTARG
CCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECC
DDRGGVVVLHHPNNQRRMSCRELAIVQGFPVDYAFFGNRSSVYRQVGNAVPPLLAYAMAK
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHH
QFNQYACE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]