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The map label for this gene is zraS [H]

Identifier: 220903470

GI number: 220903470

Start: 234547

End: 236112

Strand: Direct

Name: zraS [H]

Synonym: Ddes_0186

Alternate gene names: 220903470

Gene position: 234547-236112 (Clockwise)

Preceding gene: 220903468

Following gene: 220903471

Centisome position: 8.16

GC content: 60.47

Gene sequence:

>1566_bases
ATGGCCTCCGCCCGGTGCATGATGACACTGCGCGCGCATGACGCTATAAGCACAGGCATGACCTCGGCACACAACAACCC
GGACGGCGAAAGCCGGAAAGAGCCGCCCCAGGGTTGGGGAGGACTTGAGCTTTACGCATCCATGAAGGGGATGCGGGGCG
TTTCACGTACGCCCCTCGGCTTGCTTATGGGCGGCGCGGCCCTTGTGCTGGCTCTCATGATCAGCCTGCTGGCCTATATA
TCCATCGAGCGCAGCGAGGCTGCCATGGCCCGATTGCTCGCGGAAAAAGGCTCTTCCCTCATTATTGCCTTTGAAAGCAT
TTTGCGCTCCGGCATGCGCAGCGAGGCGGGCGTGCGCCTGCAGGTGCTGCTGGAAGAAATGGCGGCAAGCCCAGGCATTG
TTTTTGTTTCCGTAACCATGCCTGACGGCATTATTGTGGCGCACAGCAACCGTGTGCGCCTTGGAGAAATACTGAAAGTT
GAAGACAGGGAGATGGACGAGACCCGTATGCGTGACCTGAACCCGGGCATGCTGGCCCAATGGGGCATCATGCAAATGGA
GGGGCAGCGCGTTTTTGTGGTGTACCGCCAGTTTTCCCCCGGCATGGCCGACATGCCCAGAGGCTATCCTGTGCCGATCA
TTTTTCTCGGGCTGGAGATTTCGCCTTTTGAAATCACGCGCAGCCAGAACCGGGACTATGTGGCCATGCTTTCGTCCGTG
ACCCTTCTGGTGGGTCTGGCCTGTCTGTTGGCGCTGTATTATGCCGAACGGGCGCGCGAATCGCGCCAGCAGCAACGCAA
GGCCGAAGTCGAGGTGCGCCGTCTTGAAGCCGAGGTGCGCCGCAAGGAAAAGCTGGCTGCTGTGGGCAATCTGGCCGCCG
GGGTGGCTCACGAGATCCGCAATCCCTTGAGTTCCATTAAGGGTTATGCCACCTATTTCGGCCAGCGCTTTCCTGAGGGC
AGCGAAGACCGCGCTGCGGCCAATGTGATGGTTAGCGAGGTCGACAGGCTCAACCGCGTCATTATGGATCTTATCGGCCT
TTCACGGCCGAGCGATGTCTGCCCCCGGCCCGGGCGTCTTGCCGATGTGGTGGAACACGTTATGCGCCTTATCCGGCAGG
ATGCAGAAAAGCGCGGTGTTGAGGTGGAATGCCGGGTGGCCCCGGGCGTACCGCTGGCTATGGTCGATCCGGAACGTATG
GGGCAGGCCCTGCTGAATCTTTGCCTCAACGCGCTGGACGCCATGCCGGAGGGCGGACGGCTCACCTTTGCCGTGACGTG
GCATAAAAAAGGCGTATGTCTTATGGTGCGGGATACGGGCGTGGGGATTGTTCCCCGGCAGTTGCCGCATGTCTTTGACC
CCTACTTCACGACCAAGGGGCAAGGAACCGGTCTTGGGCTGGCCATGGTCCACAAGATCGTGGAGGCGCATAACGGCGAG
ATCAGCGTTACTTCCCGTCAGGCGCAGTCCGGCGCGGCGGGCGAAACCATCTTTCGAATCTGGCTGCCGGAGGCGCCTCC
GGCAACACCGCCTTTTGTGGAACGCAGAAAGAGAAAGCGTCTGTGA

Upstream 100 bases:

>100_bases
CGTCAGACGCCGTATATTCAGGCCGGAAACGGCAGAAGCGTTGCCGTGACAACGCACGTTCTGCATCTTATGCATTTGCA
AAAATCATGCAACTTTTTTT

Downstream 100 bases:

>100_bases
TTCACCGGGCTGCGCGCGGCAAATGGCTGCGACCCGGCTGGTCAGGAGGATTATCAGGGTGAAGAACAGCATTCTTGTAG
TGGACGATGACGACGCGCAC

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MASARCMMTLRAHDAISTGMTSAHNNPDGESRKEPPQGWGGLELYASMKGMRGVSRTPLGLLMGGAALVLALMISLLAYI
SIERSEAAMARLLAEKGSSLIIAFESILRSGMRSEAGVRLQVLLEEMAASPGIVFVSVTMPDGIIVAHSNRVRLGEILKV
EDREMDETRMRDLNPGMLAQWGIMQMEGQRVFVVYRQFSPGMADMPRGYPVPIIFLGLEISPFEITRSQNRDYVAMLSSV
TLLVGLACLLALYYAERARESRQQQRKAEVEVRRLEAEVRRKEKLAAVGNLAAGVAHEIRNPLSSIKGYATYFGQRFPEG
SEDRAAANVMVSEVDRLNRVIMDLIGLSRPSDVCPRPGRLADVVEHVMRLIRQDAEKRGVEVECRVAPGVPLAMVDPERM
GQALLNLCLNALDAMPEGGRLTFAVTWHKKGVCLMVRDTGVGIVPRQLPHVFDPYFTTKGQGTGLGLAMVHKIVEAHNGE
ISVTSRQAQSGAAGETIFRIWLPEAPPATPPFVERRKRKRL

Sequences:

>Translated_521_residues
MASARCMMTLRAHDAISTGMTSAHNNPDGESRKEPPQGWGGLELYASMKGMRGVSRTPLGLLMGGAALVLALMISLLAYI
SIERSEAAMARLLAEKGSSLIIAFESILRSGMRSEAGVRLQVLLEEMAASPGIVFVSVTMPDGIIVAHSNRVRLGEILKV
EDREMDETRMRDLNPGMLAQWGIMQMEGQRVFVVYRQFSPGMADMPRGYPVPIIFLGLEISPFEITRSQNRDYVAMLSSV
TLLVGLACLLALYYAERARESRQQQRKAEVEVRRLEAEVRRKEKLAAVGNLAAGVAHEIRNPLSSIKGYATYFGQRFPEG
SEDRAAANVMVSEVDRLNRVIMDLIGLSRPSDVCPRPGRLADVVEHVMRLIRQDAEKRGVEVECRVAPGVPLAMVDPERM
GQALLNLCLNALDAMPEGGRLTFAVTWHKKGVCLMVRDTGVGIVPRQLPHVFDPYFTTKGQGTGLGLAMVHKIVEAHNGE
ISVTSRQAQSGAAGETIFRIWLPEAPPATPPFVERRKRKRL
>Mature_520_residues
ASARCMMTLRAHDAISTGMTSAHNNPDGESRKEPPQGWGGLELYASMKGMRGVSRTPLGLLMGGAALVLALMISLLAYIS
IERSEAAMARLLAEKGSSLIIAFESILRSGMRSEAGVRLQVLLEEMAASPGIVFVSVTMPDGIIVAHSNRVRLGEILKVE
DREMDETRMRDLNPGMLAQWGIMQMEGQRVFVVYRQFSPGMADMPRGYPVPIIFLGLEISPFEITRSQNRDYVAMLSSVT
LLVGLACLLALYYAERARESRQQQRKAEVEVRRLEAEVRRKEKLAAVGNLAAGVAHEIRNPLSSIKGYATYFGQRFPEGS
EDRAAANVMVSEVDRLNRVIMDLIGLSRPSDVCPRPGRLADVVEHVMRLIRQDAEKRGVEVECRVAPGVPLAMVDPERMG
QALLNLCLNALDAMPEGGRLTFAVTWHKKGVCLMVRDTGVGIVPRQLPHVFDPYFTTKGQGTGLGLAMVHKIVEAHNGEI
SVTSRQAQSGAAGETIFRIWLPEAPPATPPFVERRKRKRL

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=481, Percent_Identity=37.006237006237, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788549, Length=207, Percent_Identity=34.2995169082126, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790300, Length=242, Percent_Identity=30.9917355371901, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786783, Length=335, Percent_Identity=28.0597014925373, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=238, Percent_Identity=28.1512605042017, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786600, Length=210, Percent_Identity=25.2380952380952, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789149, Length=268, Percent_Identity=24.6268656716418, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=213, Percent_Identity=29.5774647887324, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790346, Length=222, Percent_Identity=26.5765765765766, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=26.7281105990783, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=202, Percent_Identity=27.7227722772277, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=25, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57309; Mature: 57178

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASARCMMTLRAHDAISTGMTSAHNNPDGESRKEPPQGWGGLELYASMKGMRGVSRTPLG
CCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
LLMGGAALVLALMISLLAYISIERSEAAMARLLAEKGSSLIIAFESILRSGMRSEAGVRL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHH
QVLLEEMAASPGIVFVSVTMPDGIIVAHSNRVRLGEILKVEDREMDETRMRDLNPGMLAQ
HHHHHHHCCCCCEEEEEEECCCCEEEECCCCEEHHHHEECCCCCCHHHHHHCCCCCHHHH
WGIMQMEGQRVFVVYRQFSPGMADMPRGYPVPIIFLGLEISPFEITRSQNRDYVAMLSSV
CCCEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEECEECCEEEECCCCCCHHHHHHHH
TLLVGLACLLALYYAERARESRQQQRKAEVEVRRLEAEVRRKEKLAAVGNLAAGVAHEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NPLSSIKGYATYFGQRFPEGSEDRAAANVMVSEVDRLNRVIMDLIGLSRPSDVCPRPGRL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ADVVEHVMRLIRQDAEKRGVEVECRVAPGVPLAMVDPERMGQALLNLCLNALDAMPEGGR
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCE
LTFAVTWHKKGVCLMVRDTGVGIVPRQLPHVFDPYFTTKGQGTGLGLAMVHKIVEAHNGE
EEEEEEEECCCEEEEEECCCCCCCCHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCE
ISVTSRQAQSGAAGETIFRIWLPEAPPATPPFVERRKRKRL
EEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ASARCMMTLRAHDAISTGMTSAHNNPDGESRKEPPQGWGGLELYASMKGMRGVSRTPLG
CCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
LLMGGAALVLALMISLLAYISIERSEAAMARLLAEKGSSLIIAFESILRSGMRSEAGVRL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHH
QVLLEEMAASPGIVFVSVTMPDGIIVAHSNRVRLGEILKVEDREMDETRMRDLNPGMLAQ
HHHHHHHCCCCCEEEEEEECCCCEEEECCCCEEHHHHEECCCCCCHHHHHHCCCCCHHHH
WGIMQMEGQRVFVVYRQFSPGMADMPRGYPVPIIFLGLEISPFEITRSQNRDYVAMLSSV
CCCEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEECEECCEEEECCCCCCHHHHHHHH
TLLVGLACLLALYYAERARESRQQQRKAEVEVRRLEAEVRRKEKLAAVGNLAAGVAHEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NPLSSIKGYATYFGQRFPEGSEDRAAANVMVSEVDRLNRVIMDLIGLSRPSDVCPRPGRL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ADVVEHVMRLIRQDAEKRGVEVECRVAPGVPLAMVDPERMGQALLNLCLNALDAMPEGGR
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCE
LTFAVTWHKKGVCLMVRDTGVGIVPRQLPHVFDPYFTTKGQGTGLGLAMVHKIVEAHNGE
EEEEEEEECCCEEEEEECCCCCCCCHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCE
ISVTSRQAQSGAAGETIFRIWLPEAPPATPPFVERRKRKRL
EEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]