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The map label for this gene is algI [H]

Identifier: 220903464

GI number: 220903464

Start: 226543

End: 228207

Strand: Reverse

Name: algI [H]

Synonym: Ddes_0180

Alternate gene names: 220903464

Gene position: 228207-226543 (Counterclockwise)

Preceding gene: 220903465

Following gene: 220903463

Centisome position: 7.94

GC content: 59.94

Gene sequence:

>1665_bases
ATGCTGTTCAATTCCTATTCCTTTCTTTTTCTGTTTTTGCCTCTTCTGCTGCTGTGCTGGCGGCTGGCGGCGGGTTTCGG
CCCCGCACGGCTGAGTCTGGTTCTGCTGCTCTTTTCCACCGTATTTTATGCCCTCTGGGGCCTTCCCTTTCTGCTGCTTC
TGGCGGGCATACTTATCATGAACTACGCCTTTGCCCTGGCACTGTCCGCGCCGGAAAACGCAGCCGCGGCTGCCACCGGC
ACTGACGGCGCAGCTCATGCAGCGCCCCCCTATGCCGAAGACGGGACGGACGGGCAAACAACCGGCAGCCCGCGCAACCG
CCGACAACTTTTCAGTAACGGCGGGCTTAAACGCCTGGGCTGCTGGGCATGCAGCCGCAAGGGACTGCTGACCCTGGCCC
TGATCCTGAACCTGCTGCCTCTGCTCTGGTTCAAGTATTCATGGTTTTTCGCCCAGAACCTGGCCCTGCTTATGGGCGCG
GAGTGGAATTTCACCCCGCCGGGTCTGCCGCTCGGCATTTCTTTTTACACCTTTATACAGATCGCCTGGCTCGTCAGCGT
CTACCGGCGGCAGGTGACCCCCCAGGGATTTTCACGACACGCGCTTTTTTCGGCCTGTTTTCCCTACGTGATTTCCGGTC
CCATCGTGCGTTATGAGCAGATGGGTCCGCAGCTTGACGAGCTTTCCGGCTCCACCTCCGAAGGGCTGGCCCAGGGATTC
ACCCTGTTCACCATAGGACTTGCCAAAAAAGTGCTGCTGGCCGACGGCCTGGCCGTGTACGCCAACGCCGTGTTCAATGC
GGCGGAAAAAGCCTTTCCCGTCAGCGGGGCCGAGGCCTGGCTGGGTTCTTTCTGCTATACTTTTCAGCTGTACTTCGATT
TTTCCGGCTATACCGATATGGCCATCGGCCTTGGCCTCATGCTGGGGCTGCGGCTGCCGGAAAACTTTGATTCGCCCTAC
AAGGCAACGGGCATTGTTGACTTCTGGCGGCGCTGGCACATCACCCTGAGTTCCTGGCTGCGGGACTTTCTGTACATCCC
CCTGGGCGGTAACCGAAAAGGGCGGCTCATGCAGTACCGCAACCTCTTTCTGACCATGCTCATAGGTGGGGCCTGGCACG
GCGCTGGCTGGACATTCATCATATGGGGCGCGCTGCACGGGGCCATGCTGAGTATCAATCACTTTTTCCGCGCCTGCATC
AAGGGCAGCCTGACGGAGCGGGTTCTGGCTGCCCTGCCCTTGCGCATCTTCTTCATTTTTTTCACGTTCATGTGCATCAA
CCTGTGTTGGGTGGTCTTTCGCACCGTAAGCCTTGACGGTGCGGTGACCATGTACGGGGCCATGTTTGCCGGGCCTTTCA
CGGCTGAAGCCGCCGGCCTTGCTGCGGACCACACCGGGCTTTCGGCAATGGGTGCACTGGCCGCCCGCTGGCTGCCCAAC
AACTATTTTCAGGGCTGGATGCCCTTTGCCCTGCTTGGCATAAGCCTTGTGCTTGTATGGGCCTTCCCCAACAGCCACGA
ACTGCTGCACGGCAAGCGCGACGGCACGCGCCCGCTGCTGAGCTGGCGGCCATCAGCGGCATGGGCAACGGGCCTGGCCT
GTCTGGCGTTCGTGACCCTTGTTCTGGTGTCGCGCAAGGCGACCTTCCTGTACTTTCAATTCTAG

Upstream 100 bases:

>100_bases
TTGTGCCCAGCGTCTGCCGTAGCGGGCTTGTGCTGCTGTGGAGCTCCGGCCTTTTTTCGCCGGGGGCTTCCGGAGTCCGC
GTTCCGGGGGGAGGCTGCGT

Downstream 100 bases:

>100_bases
AGGGGCGGACACATCATGAGCAAAATTCTCACCGAAGCCGCCTACCTCCACCGCTACTTCCGTGTGCTGCTGCTTCTTGC
CGTGGCGGCCTGCCTGCTGG

Product: membrane bound O-acyl transferase MBOAT family protein

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MLFNSYSFLFLFLPLLLLCWRLAAGFGPARLSLVLLLFSTVFYALWGLPFLLLLAGILIMNYAFALALSAPENAAAAATG
TDGAAHAAPPYAEDGTDGQTTGSPRNRRQLFSNGGLKRLGCWACSRKGLLTLALILNLLPLLWFKYSWFFAQNLALLMGA
EWNFTPPGLPLGISFYTFIQIAWLVSVYRRQVTPQGFSRHALFSACFPYVISGPIVRYEQMGPQLDELSGSTSEGLAQGF
TLFTIGLAKKVLLADGLAVYANAVFNAAEKAFPVSGAEAWLGSFCYTFQLYFDFSGYTDMAIGLGLMLGLRLPENFDSPY
KATGIVDFWRRWHITLSSWLRDFLYIPLGGNRKGRLMQYRNLFLTMLIGGAWHGAGWTFIIWGALHGAMLSINHFFRACI
KGSLTERVLAALPLRIFFIFFTFMCINLCWVVFRTVSLDGAVTMYGAMFAGPFTAEAAGLAADHTGLSAMGALAARWLPN
NYFQGWMPFALLGISLVLVWAFPNSHELLHGKRDGTRPLLSWRPSAAWATGLACLAFVTLVLVSRKATFLYFQF

Sequences:

>Translated_554_residues
MLFNSYSFLFLFLPLLLLCWRLAAGFGPARLSLVLLLFSTVFYALWGLPFLLLLAGILIMNYAFALALSAPENAAAAATG
TDGAAHAAPPYAEDGTDGQTTGSPRNRRQLFSNGGLKRLGCWACSRKGLLTLALILNLLPLLWFKYSWFFAQNLALLMGA
EWNFTPPGLPLGISFYTFIQIAWLVSVYRRQVTPQGFSRHALFSACFPYVISGPIVRYEQMGPQLDELSGSTSEGLAQGF
TLFTIGLAKKVLLADGLAVYANAVFNAAEKAFPVSGAEAWLGSFCYTFQLYFDFSGYTDMAIGLGLMLGLRLPENFDSPY
KATGIVDFWRRWHITLSSWLRDFLYIPLGGNRKGRLMQYRNLFLTMLIGGAWHGAGWTFIIWGALHGAMLSINHFFRACI
KGSLTERVLAALPLRIFFIFFTFMCINLCWVVFRTVSLDGAVTMYGAMFAGPFTAEAAGLAADHTGLSAMGALAARWLPN
NYFQGWMPFALLGISLVLVWAFPNSHELLHGKRDGTRPLLSWRPSAAWATGLACLAFVTLVLVSRKATFLYFQF
>Mature_554_residues
MLFNSYSFLFLFLPLLLLCWRLAAGFGPARLSLVLLLFSTVFYALWGLPFLLLLAGILIMNYAFALALSAPENAAAAATG
TDGAAHAAPPYAEDGTDGQTTGSPRNRRQLFSNGGLKRLGCWACSRKGLLTLALILNLLPLLWFKYSWFFAQNLALLMGA
EWNFTPPGLPLGISFYTFIQIAWLVSVYRRQVTPQGFSRHALFSACFPYVISGPIVRYEQMGPQLDELSGSTSEGLAQGF
TLFTIGLAKKVLLADGLAVYANAVFNAAEKAFPVSGAEAWLGSFCYTFQLYFDFSGYTDMAIGLGLMLGLRLPENFDSPY
KATGIVDFWRRWHITLSSWLRDFLYIPLGGNRKGRLMQYRNLFLTMLIGGAWHGAGWTFIIWGALHGAMLSINHFFRACI
KGSLTERVLAALPLRIFFIFFTFMCINLCWVVFRTVSLDGAVTMYGAMFAGPFTAEAAGLAADHTGLSAMGALAARWLPN
NYFQGWMPFALLGISLVLVWAFPNSHELLHGKRDGTRPLLSWRPSAAWATGLACLAFVTLVLVSRKATFLYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 61273; Mature: 61273

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCEEEEEEC
>Mature Secondary Structure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HHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]