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The map label for this gene is pntA [H]

Identifier: 220903459

GI number: 220903459

Start: 215970

End: 217142

Strand: Direct

Name: pntA [H]

Synonym: Ddes_0173

Alternate gene names: 220903459

Gene position: 215970-217142 (Clockwise)

Preceding gene: 220903458

Following gene: 220903460

Centisome position: 7.52

GC content: 60.36

Gene sequence:

>1173_bases
ATGAAGTTTCAGGGTATGACTATCGGTGTGCCGAAGGAAATCATGCACGGCGAACGCCGCGTGTCCGCGACTCCCGACAC
CGTCAAGAAGATGGTTGCCGACGGCGCCACGGTTCTTGTTGAAAAGGGTGCCGGCGACGGCGCGTTCTTTGCTGACAGCG
CCTATGTTGAAGCCGGGGGCAAGATCATCGAGGACGTTCAGGAAATCTTTGCCAAGGCTGACGTTATCCTGAAGGTGAAA
GAGCCTCTGTTCAACGACAAGGTGAACAAGCACGAAGCGGAAATGGTGCGTGACGGGCAGTATCTGATCACGTTCCTGCA
CCCCGCGGCTCCCGTGAACCACGAAATGATGAAAAAGCTGGCCGCCACGGGCGTGATCAGCATTACACTGGATGGTATTC
CGCGCATTTCGCGTGCTCAGGGCATGGACGCTTTGACCTCCATGAGCACGGTTGCCGGGTACAAGGGCGTGCTTATGGCG
GCCAACCGGCTTGCCAAGTTCATGCCTATGGTTGGTACGGCCGTGGGGGTCATCAAGCCTGCCAACGTGCTGGTGATCGG
CACGGGCGTGGCCGGGCTTCAGGCCGTGGCCACTGCCAAGCGCCTGGGCGCTGTTGTCACGGCTGTGGACATCCGGCCCG
ACGCGCGCGAGCAGTCCATGAGCCTTGGCGCCAAGCCCTTTGATGTGGGTGTGCCCGCCGAGGTGGCCATTGGCGAGGGC
GGCTACGCCCAGCGCCTGAGCGACGAATGGCTGAACAAGGAACGCGAGGCCCTGAAGCCCCTGGTGAAGGACGCCGACAT
CATCATCCTTTCGGCCCTGATCCCCGGCAAGCTGGCCCCGATCCTGATCACCAAGGAAATGGTGGCTTCTATGGCTCCCG
GTTCCAGCATCGTGGACATTTCCATTGACCAGGGCGGCAACTGCGAGCTGACCGAAGCCGGTGAAGTGGTGGTCAAGCAC
GGCGTGACCATCGACGGCACCAAGAACATCCCCGGCATGATGCCCACAAGCTCCACCTGGATGTTTGCCAACAACGTGTA
CCAGCTGCTTGCCTTCCTTGCTCAGGACGGCAAGATCGTTCTGGACAGGACCGACCCCATCATCGAATCCACGCTGAGCA
CCATCGACAAGCAGATTGTCCACCGTGGCGCTCGTGAAGCCATGGGCCTTTAG

Upstream 100 bases:

>100_bases
GTTCAGATGCTTTGAAATGTTTGGGGCGAGAGCGTACAGGACGCTGGATGCCCGTATATGATGCAACAGGTTTATACAAT
TACCTTCAAGGAGCCTTATA

Downstream 100 bases:

>100_bases
TCGTTAAAGAACGCCGGGCGCGGCCTGTTCCGCGTCCGGCGTTCCGGCATACAGGCCGGGAAAAATATTATCCGAGGAAG
ACATATGACCCCATTTACCC

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKFQGMTIGVPKEIMHGERRVSATPDTVKKMVADGATVLVEKGAGDGAFFADSAYVEAGGKIIEDVQEIFAKADVILKVK
EPLFNDKVNKHEAEMVRDGQYLITFLHPAAPVNHEMMKKLAATGVISITLDGIPRISRAQGMDALTSMSTVAGYKGVLMA
ANRLAKFMPMVGTAVGVIKPANVLVIGTGVAGLQAVATAKRLGAVVTAVDIRPDAREQSMSLGAKPFDVGVPAEVAIGEG
GYAQRLSDEWLNKEREALKPLVKDADIIILSALIPGKLAPILITKEMVASMAPGSSIVDISIDQGGNCELTEAGEVVVKH
GVTIDGTKNIPGMMPTSSTWMFANNVYQLLAFLAQDGKIVLDRTDPIIESTLSTIDKQIVHRGAREAMGL

Sequences:

>Translated_390_residues
MKFQGMTIGVPKEIMHGERRVSATPDTVKKMVADGATVLVEKGAGDGAFFADSAYVEAGGKIIEDVQEIFAKADVILKVK
EPLFNDKVNKHEAEMVRDGQYLITFLHPAAPVNHEMMKKLAATGVISITLDGIPRISRAQGMDALTSMSTVAGYKGVLMA
ANRLAKFMPMVGTAVGVIKPANVLVIGTGVAGLQAVATAKRLGAVVTAVDIRPDAREQSMSLGAKPFDVGVPAEVAIGEG
GYAQRLSDEWLNKEREALKPLVKDADIIILSALIPGKLAPILITKEMVASMAPGSSIVDISIDQGGNCELTEAGEVVVKH
GVTIDGTKNIPGMMPTSSTWMFANNVYQLLAFLAQDGKIVLDRTDPIIESTLSTIDKQIVHRGAREAMGL
>Mature_390_residues
MKFQGMTIGVPKEIMHGERRVSATPDTVKKMVADGATVLVEKGAGDGAFFADSAYVEAGGKIIEDVQEIFAKADVILKVK
EPLFNDKVNKHEAEMVRDGQYLITFLHPAAPVNHEMMKKLAATGVISITLDGIPRISRAQGMDALTSMSTVAGYKGVLMA
ANRLAKFMPMVGTAVGVIKPANVLVIGTGVAGLQAVATAKRLGAVVTAVDIRPDAREQSMSLGAKPFDVGVPAEVAIGEG
GYAQRLSDEWLNKEREALKPLVKDADIIILSALIPGKLAPILITKEMVASMAPGSSIVDISIDQGGNCELTEAGEVVVKH
GVTIDGTKNIPGMMPTSSTWMFANNVYQLLAFLAQDGKIVLDRTDPIIESTLSTIDKQIVHRGAREAMGL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=354, Percent_Identity=40.1129943502825, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI122939153, Length=354, Percent_Identity=40.1129943502825, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1787887, Length=367, Percent_Identity=42.7792915531335, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17550456, Length=379, Percent_Identity=40.3693931398417, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 41359; Mature: 41359

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQGMTIGVPKEIMHGERRVSATPDTVKKMVADGATVLVEKGAGDGAFFADSAYVEAGG
CCCCCCEECCCHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHCCC
KIIEDVQEIFAKADVILKVKEPLFNDKVNKHEAEMVRDGQYLITFLHPAAPVNHEMMKKL
HHHHHHHHHHHHCCEEEEECCCHHCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
AATGVISITLDGIPRISRAQGMDALTSMSTVAGYKGVLMAANRLAKFMPMVGTAVGVIKP
HHCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ANVLVIGTGVAGLQAVATAKRLGAVVTAVDIRPDAREQSMSLGAKPFDVGVPAEVAIGEG
CCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEECCC
GYAQRLSDEWLNKEREALKPLVKDADIIILSALIPGKLAPILITKEMVASMAPGSSIVDI
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEEHHHHHHCCCCCEEEEE
SIDQGGNCELTEAGEVVVKHGVTIDGTKNIPGMMPTSSTWMFANNVYQLLAFLAQDGKIV
EECCCCCEEEECCCCEEEECCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEE
LDRTDPIIESTLSTIDKQIVHRGAREAMGL
EECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFQGMTIGVPKEIMHGERRVSATPDTVKKMVADGATVLVEKGAGDGAFFADSAYVEAGG
CCCCCCEECCCHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHCCC
KIIEDVQEIFAKADVILKVKEPLFNDKVNKHEAEMVRDGQYLITFLHPAAPVNHEMMKKL
HHHHHHHHHHHHCCEEEEECCCHHCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
AATGVISITLDGIPRISRAQGMDALTSMSTVAGYKGVLMAANRLAKFMPMVGTAVGVIKP
HHCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ANVLVIGTGVAGLQAVATAKRLGAVVTAVDIRPDAREQSMSLGAKPFDVGVPAEVAIGEG
CCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEECCC
GYAQRLSDEWLNKEREALKPLVKDADIIILSALIPGKLAPILITKEMVASMAPGSSIVDI
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEEHHHHHHCCCCCEEEEE
SIDQGGNCELTEAGEVVVKHGVTIDGTKNIPGMMPTSSTWMFANNVYQLLAFLAQDGKIV
EECCCCCEEEECCCCEEEECCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEE
LDRTDPIIESTLSTIDKQIVHRGAREAMGL
EECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]