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The map label for this gene is lpdA [H]

Identifier: 220903437

GI number: 220903437

Start: 189130

End: 190533

Strand: Reverse

Name: lpdA [H]

Synonym: Ddes_0151

Alternate gene names: 220903437

Gene position: 190533-189130 (Counterclockwise)

Preceding gene: 220903438

Following gene: 220903435

Centisome position: 6.63

GC content: 61.75

Gene sequence:

>1404_bases
ATGAAAAAACTGACCATCATCGGGAGCGGCCCCGGCGGCCACATGGCGGCCTTTGCGGCGGCCCGCGCCGGGCACAGCGT
CACCCTTGTGGAAAGCGCGGCCGTGGGCGGCACATGCCTGCACACGGGCTGCATCCCCACCAAGACCTTCAAAAGCTCTG
CCGATGCCCTTGAAAAAATACGCCATATGGGCAACTACGCCATCACCGGCAATGCTGATGCCTCCATATCCATGCCCGAC
CTGCTGGCGCGCAAAAAAAACGTCATCGGTCTTCTGACCACCGGCCTTGAAAAAACCTGCGCCAGCCTCAAGATCACCAT
CGTGCGCGGGCGCGGCAGGGTTGTCAGCGCCAGGGAAGTGGCCGTAAGCACGCAGGAAGGCACTGTGCTTGTAGAAAGCG
ACAACGTCATCATCGCTACCGGCTCACGCCCGCTCGACCTGCCCTCCCTGCCTGCCGATCACAAACACATCCTCAACAGC
GACGACGCTCTTGCCCTGGAGCATGTGCCTACCAGCATCGCCATTGTGGGCGGCGGCGTTATCGGTTGCGAAATGGCCTG
CATTTTCCGCTCCTTCGGCGCACAGGTCACTGTCATTGAAGGACAGGACCGCCTGCTGCCCATCCCTTCGGTGGATGCCG
AAATGAGCAAGCTGCTGCTGCGCGAGATGAAAAAGGCGGGCATACGTGTGGAGCTTGGCAAAACTGTCAGTGCCGCCACT
GTGGAACAGGGCGTGGCAAAACTTGCCGTAAGCCCCTCGCGGCCCAGGGGCGATGCCCCGGCTGAAGCGGCCATGCTGGA
TGTGGAGTCGGTTTTTGTCACCGTGGGCCGGGTGCCCAACACTGAAGGCCTCGGCCTTGCCGAAGCGGGCATTGCCGTGA
CGCCGCGCGGCTGGATTGAGGTGGACGACTGCCTGCAAACCTCTGTTCCCGGTGTGTTTGCCATTGGCGACGTTCTCGGC
CCGGAAAAAATCATGCTGGCCCACATGGCCTCTGCCGAAGCCGAGCATGTGGTGGCCCACCTTGATTCTCCCGCCCCCTG
CGACTACAGCGTTGTGCCTTCGGCCATTTTCACCACGCCTGAAATCGGCTGCGTGGGTCTTTCTGAAGAACAGGCCCTGG
CCGCCGGTCTGCCTGTCCGCTGCACGCTTTTGCAGGTAAGGGAACTCGGCAAGGCCCACGCAATGGGAGAAATCGCCGGG
GTATTCAAGCTTGTGGCCCATGCTGAAAACAATACCATTCTTGGCGCGCACCTCTGCGGGCCGCACGCCACGGACCTTGT
CGCCGAGGCCGGGCTGGCAATCCGCATGGGAGCCAAGGTTGACGATGTGGCGCACACCATTCACGCCCATCCCACCCTGG
CCGAAGGGTTTTACGAACTTTGCCACAAGGCCGCACGGCCATAA

Upstream 100 bases:

>100_bases
CTTATGGTATGCCACTGAAAACAACGCCCCATCGTGATGCACTCCCCAGGCAAAAAGCCCTGGATCATAAAACCTGCGCA
AGCCAGCACGAGGTAACGAT

Downstream 100 bases:

>100_bases
CCCTTCTGGAGCATTTTCACTGCCAACCCGCTCTGGCAGGCCTTGGAGCATTTCACACCTGAAATGCTCTACAATGCTCT
ACAAGGATTTGTTTCCAGAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKKLTIIGSGPGGHMAAFAAARAGHSVTLVESAAVGGTCLHTGCIPTKTFKSSADALEKIRHMGNYAITGNADASISMPD
LLARKKNVIGLLTTGLEKTCASLKITIVRGRGRVVSAREVAVSTQEGTVLVESDNVIIATGSRPLDLPSLPADHKHILNS
DDALALEHVPTSIAIVGGGVIGCEMACIFRSFGAQVTVIEGQDRLLPIPSVDAEMSKLLLREMKKAGIRVELGKTVSAAT
VEQGVAKLAVSPSRPRGDAPAEAAMLDVESVFVTVGRVPNTEGLGLAEAGIAVTPRGWIEVDDCLQTSVPGVFAIGDVLG
PEKIMLAHMASAEAEHVVAHLDSPAPCDYSVVPSAIFTTPEIGCVGLSEEQALAAGLPVRCTLLQVRELGKAHAMGEIAG
VFKLVAHAENNTILGAHLCGPHATDLVAEAGLAIRMGAKVDDVAHTIHAHPTLAEGFYELCHKAARP

Sequences:

>Translated_467_residues
MKKLTIIGSGPGGHMAAFAAARAGHSVTLVESAAVGGTCLHTGCIPTKTFKSSADALEKIRHMGNYAITGNADASISMPD
LLARKKNVIGLLTTGLEKTCASLKITIVRGRGRVVSAREVAVSTQEGTVLVESDNVIIATGSRPLDLPSLPADHKHILNS
DDALALEHVPTSIAIVGGGVIGCEMACIFRSFGAQVTVIEGQDRLLPIPSVDAEMSKLLLREMKKAGIRVELGKTVSAAT
VEQGVAKLAVSPSRPRGDAPAEAAMLDVESVFVTVGRVPNTEGLGLAEAGIAVTPRGWIEVDDCLQTSVPGVFAIGDVLG
PEKIMLAHMASAEAEHVVAHLDSPAPCDYSVVPSAIFTTPEIGCVGLSEEQALAAGLPVRCTLLQVRELGKAHAMGEIAG
VFKLVAHAENNTILGAHLCGPHATDLVAEAGLAIRMGAKVDDVAHTIHAHPTLAEGFYELCHKAARP
>Mature_467_residues
MKKLTIIGSGPGGHMAAFAAARAGHSVTLVESAAVGGTCLHTGCIPTKTFKSSADALEKIRHMGNYAITGNADASISMPD
LLARKKNVIGLLTTGLEKTCASLKITIVRGRGRVVSAREVAVSTQEGTVLVESDNVIIATGSRPLDLPSLPADHKHILNS
DDALALEHVPTSIAIVGGGVIGCEMACIFRSFGAQVTVIEGQDRLLPIPSVDAEMSKLLLREMKKAGIRVELGKTVSAAT
VEQGVAKLAVSPSRPRGDAPAEAAMLDVESVFVTVGRVPNTEGLGLAEAGIAVTPRGWIEVDDCLQTSVPGVFAIGDVLG
PEKIMLAHMASAEAEHVVAHLDSPAPCDYSVVPSAIFTTPEIGCVGLSEEQALAAGLPVRCTLLQVRELGKAHAMGEIAG
VFKLVAHAENNTILGAHLCGPHATDLVAEAGLAIRMGAKVDDVAHTIHAHPTLAEGFYELCHKAARP

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=35.0649350649351, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=27.9735682819383, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI291045266, Length=440, Percent_Identity=27.0454545454545, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI148277065, Length=448, Percent_Identity=26.3392857142857, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519430, Length=448, Percent_Identity=26.3392857142857, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519428, Length=448, Percent_Identity=26.3392857142857, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519426, Length=448, Percent_Identity=26.3392857142857, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277071, Length=448, Percent_Identity=26.3392857142857, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI22035672, Length=467, Percent_Identity=27.8372591006424, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI291045268, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786307, Length=456, Percent_Identity=34.4298245614035, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=29.5503211991435, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=26.6816143497758, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081717, Length=463, Percent_Identity=26.9978401727862, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=34.6072186836518, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17557007, Length=487, Percent_Identity=26.694045174538, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=444, Percent_Identity=23.6486486486486, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=454, Percent_Identity=26.6519823788546, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=454, Percent_Identity=26.6519823788546, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=33.2622601279318, Blast_Score=229, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=27.4509803921569, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=27.1398747390397, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=35.2813852813853, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=27.2727272727273, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=27.3469387755102, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640551, Length=484, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=27.9022403258656, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48408; Mature: 48408

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTIIGSGPGGHMAAFAAARAGHSVTLVESAAVGGTCLHTGCIPTKTFKSSADALEKI
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHH
RHMGNYAITGNADASISMPDLLARKKNVIGLLTTGLEKTCASLKITIVRGRGRVVSAREV
HHCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCHHHHHCEEEEEEEECCCCEEEEHEE
AVSTQEGTVLVESDNVIIATGSRPLDLPSLPADHKHILNSDDALALEHVPTSIAIVGGGV
EEEECCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEECCCCEEEEECCCH
IGCEMACIFRSFGAQVTVIEGQDRLLPIPSVDAEMSKLLLREMKKAGIRVELGKTVSAAT
HHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHH
VEQGVAKLAVSPSRPRGDAPAEAAMLDVESVFVTVGRVPNTEGLGLAEAGIAVTPRGWIE
HHHHHHHEECCCCCCCCCCCHHHHHEEHHHEEEEECCCCCCCCCCEECCCEEECCCCCEE
VDDCLQTSVPGVFAIGDVLGPEKIMLAHMASAEAEHVVAHLDSPAPCDYSVVPSAIFTTP
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHCCCC
EIGCVGLSEEQALAAGLPVRCTLLQVRELGKAHAMGEIAGVFKLVAHAENNTILGAHLCG
CCCEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
PHATDLVAEAGLAIRMGAKVDDVAHTIHAHPTLAEGFYELCHKAARP
CCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLTIIGSGPGGHMAAFAAARAGHSVTLVESAAVGGTCLHTGCIPTKTFKSSADALEKI
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHH
RHMGNYAITGNADASISMPDLLARKKNVIGLLTTGLEKTCASLKITIVRGRGRVVSAREV
HHCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCHHHHHCEEEEEEEECCCCEEEEHEE
AVSTQEGTVLVESDNVIIATGSRPLDLPSLPADHKHILNSDDALALEHVPTSIAIVGGGV
EEEECCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEECCCCEEEEECCCH
IGCEMACIFRSFGAQVTVIEGQDRLLPIPSVDAEMSKLLLREMKKAGIRVELGKTVSAAT
HHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHH
VEQGVAKLAVSPSRPRGDAPAEAAMLDVESVFVTVGRVPNTEGLGLAEAGIAVTPRGWIE
HHHHHHHEECCCCCCCCCCCHHHHHEEHHHEEEEECCCCCCCCCCEECCCEEECCCCCEE
VDDCLQTSVPGVFAIGDVLGPEKIMLAHMASAEAEHVVAHLDSPAPCDYSVVPSAIFTTP
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHCCCC
EIGCVGLSEEQALAAGLPVRCTLLQVRELGKAHAMGEIAGVFKLVAHAENNTILGAHLCG
CCCEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
PHATDLVAEAGLAIRMGAKVDDVAHTIHAHPTLAEGFYELCHKAARP
CCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]